{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,16]],"date-time":"2025-11-16T20:32:18Z","timestamp":1763325138103,"version":"3.45.0"},"reference-count":0,"publisher":"BMJ","issue":"5741","license":[{"start":{"date-parts":[[1971,1,16]],"date-time":"1971-01-16T00:00:00Z","timestamp":32832000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.bmj.com\/company\/legal-information\/terms-conditions\/legal-information\/tdm-licencepolicy"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Br Med J"],"published-print":{"date-parts":[[1971,1,16]]},"abstract":"<jats:p>Application of a fluorescence technique for the detection of the human Y chromosome in interphase nuclei indicates that the best results may be obtained from the study of lymphocytes in blood smears. The most satisfactory Y-fluorescence is obtained with the use of buffered quinacrine mustard dihydrochloride. The method can be complemented by other standard techniques to obtain a clear idea of the sex-chromosome complement of the individual. The application of this technique to clinical and chromosomal diagnosis and to the prenatal detection of the Y chromosome in amniotic cells seems at least as promising as has proved the earlier identification of the Barr body.<\/jats:p>","DOI":"10.1136\/bmj.1.5741.138","type":"journal-article","created":{"date-parts":[[2009,4,8]],"date-time":"2009-04-08T15:21:07Z","timestamp":1239204067000},"page":"138-142","source":"Crossref","is-referenced-by-count":77,"title":["Y-Fluorescence of Interphase Nuclei, Especially Circulating Lymphocytes"],"prefix":"10.1136","volume":"1","author":[{"given":"P. E.","family":"Polani","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. E.","family":"Mutton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1971,1,16]]},"container-title":["BMJ"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/data.bmj.org\/tdm\/10.1136\/bmj.1.5741.138","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/bmj.1.5741.138","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,16]],"date-time":"2025-11-16T20:30:36Z","timestamp":1763325036000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.bmj.com\/lookup\/doi\/10.1136\/bmj.1.5741.138"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1971,1,16]]},"references-count":0,"journal-issue":{"issue":"5741","published-online":{"date-parts":[[1971,1,16]]},"published-print":{"date-parts":[[1971,1,16]]}},"alternative-id":["10.1136\/bmj.1.5741.138"],"URL":"https:\/\/doi.org\/10.1136\/bmj.1.5741.138","relation":{},"ISSN":["0007-1447","1468-5833"],"issn-type":[{"type":"print","value":"0007-1447"},{"type":"electronic","value":"1468-5833"}],"subject":[],"published":{"date-parts":[[1971,1,16]]}}}