{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T20:06:48Z","timestamp":1764965208371,"version":"3.46.0"},"reference-count":0,"publisher":"BMJ","issue":"5814","license":[{"start":{"date-parts":[[1972,6,10]],"date-time":"1972-06-10T00:00:00Z","timestamp":76982400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.bmj.com\/company\/legal-information\/terms-conditions\/legal-information\/tdm-licencepolicy"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Br Med J"],"published-print":{"date-parts":[[1972,6,10]]},"abstract":"<jats:p>A new simple and sensitive in-vitro method for the diagnosis of type 1 (IgE-mediated) hypersensitivity in man is described. Sliced human skin is passively sensitized by reaginic serum from allergic patients and the presence of antigen-specific IgE on the sensitized slices is detected by assay of antigen-evoked histamine release. Serum from 12 out of 14 patients with clinical respiratory allergy and positive skin tests gave significant antigen-specific histamine release. This method, which is essentially an in-vitro model of the Prausnitz-K\u00fcstner reaction, should prove of value in the diagnosis of human reaginic hypersensitivity in man.<\/jats:p>","DOI":"10.1136\/bmj.2.5814.623","type":"journal-article","created":{"date-parts":[[2009,4,8]],"date-time":"2009-04-08T15:21:07Z","timestamp":1239204067000},"page":"623-624","source":"Crossref","is-referenced-by-count":3,"title":["New In-vitro Test for IgE-mediated Hypersensitivity in Man"],"prefix":"10.1136","volume":"2","author":[{"given":"Malcolm W.","family":"Greaves","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shoso","family":"Yamamoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valerie M.","family":"Fairley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1972,6,10]]},"container-title":["BMJ"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/data.bmj.org\/tdm\/10.1136\/bmj.2.5814.623","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/bmj.2.5814.623","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T20:01:31Z","timestamp":1764964891000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.bmj.com\/lookup\/doi\/10.1136\/bmj.2.5814.623"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1972,6,10]]},"references-count":0,"journal-issue":{"issue":"5814","published-online":{"date-parts":[[1972,6,10]]},"published-print":{"date-parts":[[1972,6,10]]}},"alternative-id":["10.1136\/bmj.2.5814.623"],"URL":"https:\/\/doi.org\/10.1136\/bmj.2.5814.623","relation":{},"ISSN":["0007-1447","1468-5833"],"issn-type":[{"type":"print","value":"0007-1447"},{"type":"electronic","value":"1468-5833"}],"subject":[],"published":{"date-parts":[[1972,6,10]]}}}