{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T03:45:39Z","timestamp":1773287139126,"version":"3.50.1"},"reference-count":13,"publisher":"BMJ","issue":"6253","license":[{"start":{"date-parts":[[1980,11,29]],"date-time":"1980-11-29T00:00:00Z","timestamp":344304000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.bmj.com\/company\/legal-information\/terms-conditions\/legal-information\/tdm-licencepolicy"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Br Med J"],"published-print":{"date-parts":[[1980,11,29]]},"abstract":"<jats:p>Rapidly adhering cells (RA cells) from the amniotic fluid of a pregnancy with fetal anencephaly were investigated by immunofluorescence assay with an antiserum against glial cells. After 24 hours' cultivation a high proportion of the cells showed positive glial-specific fluorescence, whereas no staining was seen in cells from samples of normal amniotic fluid. At the 24th week the mother was delivered of a stillborn infant with anencephaly. Immunofluorescence staining of RA cells with glial-specific antiserum may be used for the differential diagnosis of fetal abnormalities associated with a high alpha-fetoprotein concentration in amniotic fluid.<\/jats:p>","DOI":"10.1136\/bmj.281.6253.1456","type":"journal-article","created":{"date-parts":[[2009,4,2]],"date-time":"2009-04-02T18:32:30Z","timestamp":1238697150000},"page":"1456-1457","source":"Crossref","is-referenced-by-count":29,"title":["Glial origin of rapidly adhering amniotic fluid cells."],"prefix":"10.1136","volume":"281","author":[{"given":"P","family":"Aula","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H","family":"von Koskull","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Teramo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O","family":"Karjalainen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I","family":"Virtanen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V P","family":"Lehto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D","family":"Dahl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1980,11,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1098","DOI":"10.1016\/S0140-6736(73)92720-7","article-title":"Amniotic fluid macrophages and anencephaly","volume":"ii:","author":"Sutherland, G.R.; Brock, D.J.H.; Scrimgeour, J.B.","year":"1973","unstructured":"Sutherland GR, Brock DJH, Scrimgeour JB. Amniotic fluid macrophages and anencephaly. Lancet 1973;ii:1098-9.","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1016\/S0140-6736(77)92221-8","article-title":"Morphology of rapidly adhering amniotic-fluid cells as an aid to the diagnosis of neural-tube defects","volume":"i:","author":"Gosden, C.M.; Brock, D.J.H.","year":"1977","unstructured":"Gosden CM, Brock DJH. Morphology of rapidly adhering amniotic-fluid cells as an aid to the diagnosis of neural-tube defects. Lancet 1977;i: 919-22.","journal-title":"Lancet"},{"key":"ref_3","first-page":"1186","article-title":"Amniotic fluid cell morphology in early antenatal prediction of abortion and low birth weight. Br MedJ'","volume":"ii:","author":"Gosden, C.M.; Brock, D.J.H.","year":"1978","unstructured":"Gosden CM, Brock DJH. Amniotic fluid cell morphology in early antenatal prediction of abortion and low birth weight. Br MedJ' 1978 ii:1186-9."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1111\/j.1399-0004.1977.tb00926.x","article-title":"The origin of the rapidly adhering cells found in amniotic fluids from foetuses with neural tube defects","volume":"12","author":"Gosden, C.M.; Brock, D.J.H.; Eason, P.","year":"1977","unstructured":"Gosden CM, Brock DJH, Eason P. The origin of the rapidly adhering cells found in amniotic fluids from foetuses with neural tube defects. Clin Genet 1977;12:193-201.","journal-title":"Clin Genet"},{"key":"ref_5","first-page":"374","article-title":"Studies of carcino-fetal proteins: physical and chemical properties of human alpha-fetoprotein","volume":"8","author":"Ruoslahti, E.; Seppala, M.","year":"1971","unstructured":"Ruoslahti E, Seppala M. Studies of carcino-fetal proteins: physical and chemical properties of human alpha-fetoprotein. Int J Cancer 1971;8: 374-83.","journal-title":"Int J Cancer"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1177\/25.6.69656","article-title":"Specificity of the glial fibrillary acidic protein for astroglia","volume":"25","author":"Bignami, A.; Dahl, D.","year":"1977","unstructured":"Bignami A, Dahl D. Specificity of the glial fibrillary acidic protein for astroglia. J Histochem Cytochem 1977;25:466-9.","journal-title":"J Histochem Cytochem"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1007\/BF00698276","article-title":"Glial fibrillary acidic protein and intermediate filaments in human glioma cells","volume":"47","author":"Paetau, A.; Virtanen, I.; Stenman, S.","year":"1979","unstructured":"Paetau A, Virtanen I, Stenman S, et al. Glial fibrillary acidic protein and intermediate filaments in human glioma cells. Acta Neuropathol 1979;47:71-4.","journal-title":"Acta Neuropathol"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1016\/0092-8674(78)90233-7","article-title":"Immunofluorescent staining of keratin fibers in cultured cells","volume":"14","author":"Sun, T.-T.; Green, H.","year":"1978","unstructured":"Sun T-T, Green H. Immunofluorescent staining of keratin fibers in cultured cells. Cell 1978;14:469-76.","journal-title":"Cell"},{"key":"ref_9","first-page":"67","article-title":"Neural origin of cells in amniotic fluid","volume":"136","author":"Sarkar, S.; Chang, H.C.; Porreco, R.P.; Jones, O.W.","year":"1978","unstructured":"Sarkar S, Chang HC, Porreco RP, Jones OW. Neural origin of cells in amniotic fluid. Am J Obstet Gynecol 1978;136:67-72.","journal-title":"Am J Obstet Gynecol"},{"key":"ref_10","first-page":"768","article-title":"Determination of subfractions of amniotic fluid alpha-fetoprotein in diagnosing spina bifida and congenital nephrosis Br MedJ","volume":"ii:","author":"Ruoslahti, E.; Pekkala, A.; Comings, E.; Seppala, M.","year":"1979","unstructured":"Ruoslahti E, Pekkala A, Comings E, Seppala M. Determination of subfractions of amniotic fluid alpha-fetoprotein in diagnosing spina bifida and congenital nephrosis Br MedJ 1979;ii:768-9."},{"key":"ref_11","first-page":"685","article-title":"Amniotic fluid acetylcholinesterase as a possible diagnostic test for neural-tube defects in early pregnancy","author":"Smith, A.D.; Wald, N.J.; Cuckle, H.S.; Stirrat, G.M.; Bobrow, M.; Lagercrantz, H.","year":"1979","unstructured":"Smith AD, Wald NJ, Cuckle HS, Stirrat GM, Bobrow M, Lagercrantz H. Amniotic fluid acetylcholinesterase as a possible diagnostic test for neural-tube defects in early pregnancy. Lancet 1979;i-685-8.","journal-title":"Lancet"},{"key":"ref_12","first-page":"746","article-title":"Cultivated cells from diagnostic amniocentesis in second trimester pregnancies. Clonal morphology and growth potential","volume":"8","author":"Hoehn, H.; Bryant, E.M.; Karp, L.E.; Martin, M.M.","year":"1974","unstructured":"Hoehn H, Bryant EM, Karp LE, Martin MM. Cultivated cells from diagnostic amniocentesis in second trimester pregnancies. Clonal morphology and growth potential. Pediat Res 1974;8:746-54.","journal-title":"Pediat Res"},{"key":"ref_13","article-title":"Accepted 15","year":"1980","unstructured":"(Accepted 15 October 1980)"}],"container-title":["BMJ"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/data.bmj.org\/tdm\/10.1136\/bmj.281.6253.1456","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/bmj.281.6253.1456","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T13:26:27Z","timestamp":1765459587000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.bmj.com\/lookup\/doi\/10.1136\/bmj.281.6253.1456"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1980,11,29]]},"references-count":13,"journal-issue":{"issue":"6253","published-online":{"date-parts":[[1980,11,29]]},"published-print":{"date-parts":[[1980,11,29]]}},"alternative-id":["10.1136\/bmj.281.6253.1456"],"URL":"https:\/\/doi.org\/10.1136\/bmj.281.6253.1456","relation":{},"ISSN":["0007-1447","1468-5833"],"issn-type":[{"value":"0007-1447","type":"print"},{"value":"1468-5833","type":"electronic"}],"subject":[],"published":{"date-parts":[[1980,11,29]]}}}