{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T22:21:33Z","timestamp":1765405293087,"version":"3.46.0"},"reference-count":20,"publisher":"BMJ","issue":"6443","license":[{"start":{"date-parts":[[1984,8,25]],"date-time":"1984-08-25T00:00:00Z","timestamp":462240000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.bmj.com\/company\/legal-information\/terms-conditions\/legal-information\/tdm-licencepolicy"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Br Med J (Clin Res Ed)"],"published-print":{"date-parts":[[1984,8,25]]},"abstract":"<jats:p>A highly specific and sensitive radioimmunoassay was developed for measuring circulating growth hormone releasing factor (GRF) in human plasma. Before measuring immunoreactive GRF plasma samples were extracted on to Vycor glass. Immunoreactive GRF concentrations in plasma samples from 37 fasting normal subjects ranged from less than 10 to 60 ng\/l (mean 21 ng\/l). Fasting concentrations in 76 out of 80 acromegalic subjects were within the normal range, but the remaining four patients had values of 92 to 25 000 ng\/l. Of these, only the patient with the highest concentration had evidence of ectopic GRF secretion from a disseminated carcinoid tumour. Two of the others had longstanding pituitary tumours, and the fourth patient had a pituitary growth hormone (GH) secreting tumour proved by its removal and subsequent remission of acromegaly. There was no correlation between serum GH and plasma immunoreactive GRF concentrations, irrespective of whether the patients were untreated or had been given radiotherapy or dopamine agonists. The assay should help elucidate the physiological role(s) of GRF and may also prove useful in differentiating between pituitary and hypothalamic defects in patients with acromegaly.<\/jats:p>","DOI":"10.1136\/bmj.289.6443.453","type":"journal-article","created":{"date-parts":[[2009,4,2]],"date-time":"2009-04-02T11:00:59Z","timestamp":1238670059000},"page":"453-455","source":"Crossref","is-referenced-by-count":48,"title":["Circulating growth hormone releasing factor concentrations in normal subjects and patients with acromegaly."],"prefix":"10.1136","volume":"289","author":[{"given":"E S","family":"Penny","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"Penman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Price","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L H","family":"Rees","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A M","family":"Sopwith","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J A","family":"Wass","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N","family":"Lytras","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G M","family":"Besser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1984,8,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1126\/science.179.4068.77","article-title":"Hypothalamic polypeptide that inhibits the secretion of immunoreactive pituitary growth hormone","volume":"178","author":"Brazeau, P.; Vale, W.; Burgus, R.","year":"1973","unstructured":"Brazeau P, Vale W, Burgus R, et al. Hypothalamic polypeptide that inhibits the secretion of immunoreactive pituitary growth hormone. Science 1973;178:77-9.","journal-title":"Science"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1038\/300276a0","article-title":"Characterisation of a growth hormonereleasing factor from a human pancreatic islet tumour","volume":"300","author":"Rivier, J.; Spiess, J.; Thorner, M.; Vale, W.","year":"1982","unstructured":"Rivier J, Spiess J, Thorner M, Vale W. Characterisation of a growth hormonereleasing factor from a human pancreatic islet tumour. Nature 1982;300:276-8.","journal-title":"Nature"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1126\/science.6812220","article-title":"Growth hormone-releasing factor from a human pancreatic tumor that caused acromegaly","volume":"218","author":"Guillemin, R.; Brazeau, P.; Bohlen, P.; Esch, F.; Ling, N.","year":"1982","unstructured":"Guillemin R, Brazeau P, Bohlen P, Esch F, Ling N. 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Cloning and sequence analysis of cDNA for the precursor of human growth hormone-releasing factor, somatocrinin. Proc Natl Acad Sci USA 1983;80:4311-4."},{"key":"ref_6","first-page":"14","article-title":"Human hypothalamic growth hormone releasing factor (GRF): evidence for two forms identical to tumor derived GRF-44NH2 and GRF-40","volume":"1","author":"Bohlen, P.; Brazeau, P.; Bloch, B.; Ling, N.; Gaillard, R.; Guillemin, R.","year":"1983","unstructured":"Bohlen P, Brazeau P, Bloch B, Ling N, Gaillard R, Guillemin R. Human hypothalamic growth hormone releasing factor (GRF): evidence for two forms identical to tumor derived GRF-44NH2 and GRF-40. 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