{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:24:45Z","timestamp":1789431885312,"version":"build-2803163510"},"reference-count":16,"publisher":"BMJ","issue":"6456","license":[{"start":{"date-parts":[[1984,11,24]],"date-time":"1984-11-24T00:00:00Z","timestamp":470102400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.bmj.com\/company\/legal-information\/terms-conditions\/legal-information\/tdm-licencepolicy"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Br Med J (Clin Res Ed)"],"published-print":{"date-parts":[[1984,11,24]]},"abstract":"<jats:p>The isolation rate of Haemophilus influenzae from patients with persistent production of purulent sputum has been increased by the routine use of a selective medium. Nevertheless, some purulent sputum still fails to yield a pathogen. The selective medium was supplemented with N-acetyl-D-glucosamine to encourage primary isolation of colony forming, spheroplastic H influenzae, which reverted to normal forms on subculture. On the basis of in vitro experiments it is postulated that these spheroplastic forms of H influenzae may be induced by inadequate antimicrobial chemotherapy and may be responsible for re-emergence of symptoms in these patients during or shortly after stopping chemotherapy.<\/jats:p>","DOI":"10.1136\/bmj.289.6456.1409","type":"journal-article","created":{"date-parts":[[2009,4,2]],"date-time":"2009-04-02T11:00:59Z","timestamp":1238670059000},"page":"1409-1412","source":"Crossref","is-referenced-by-count":28,"title":["Isolation of spheroplastic forms of Haemophilus influenzae from sputum in conventionally treated chronic bronchial sepsis using selective medium supplemented with N-acetyl-D-glucosamine: possible reservoir for re-emergence of infection."],"prefix":"10.1136","volume":"289","author":[{"given":"D","family":"Roberts","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Higgs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Rutman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"Cole","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[1984,11,24]]},"reference":[{"key":"ref_1","first-page":"1","article-title":"A new look at the pathogenesis and management of persistent bronchial sepsis: a \"vicious circle\" hypothesis and its logical therapeutic connotations","volume-title":"Strategies for the management of chronic bronchial sepsis","author":"Cole, P.J.","year":"1984","unstructured":"Cole PJ. A new look at the pathogenesis and management of persistent bronchial sepsis: a \"vicious circle\" hypothesis and its logical therapeutic connotations. In: Davies RJ, ed. Strategies for the management of chronic bronchial sepsis. Oxford: The Medicine Publishing Foundation, 1984:1-20."},{"key":"ref_2","first-page":"21","article-title":"Microbiological investigation of sputum","volume-title":"Strategies for the management of chronic bronchial sepsis","author":"Roberts, D.E.","year":"1984","unstructured":"Roberts DE. Microbiological investigation of sputum. In: Davies RJ, ed. Strategies for the management of chronic bronchial sepsis. Oxford: The Medicine Publishing Foundation, 1984:21-8."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/S0140-6736(83)92629-6","article-title":"High dose antibiotic is logical, effective and economical in treatment of severe bronchial sepsis","volume":"i:","author":"Cole, P.J.; Roberts, D.E.","year":"1983","unstructured":"Cole PJ, Roberts DE. High dose antibiotic is logical, effective and economical in treatment of severe bronchial sepsis. Lancet 1983;i:248-9.","journal-title":"Lancet"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1136\/bmj.286.6371.1147","article-title":"Ampicillin and alternatives","volume":"286","author":"Cole, P.J.; Roberts, D.E.","year":"1983","unstructured":"Cole PJ, Roberts DE. Ampicillin and alternatives. Br Med J 1983;286:1147.","journal-title":"Br Med J"},{"key":"ref_5","first-page":"30","article-title":"Chemotherapy of chronic bronchitis and allied disorders","author":"May, JR","year":"1972","unstructured":"May JR. Chemotherapy of chronic bronchitis and allied disorders. 2nd ed. London: The English University Press Ltd, 1972:30.","edition":"2nd ed"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1093\/jac\/7.1.112","article-title":"Clavulanic acid and respiratory infection","volume":"7","author":"Roberts, D.E.; Cole, P.J.","year":"1981","unstructured":"Roberts DE, Cole PJ. Clavulanic acid and respiratory infection. J Antimicrob Chemother 1981;7:112.","journal-title":"J Antimicrob Chemother"},{"key":"ref_7","article-title":"Immunity deficiency and the lung","volume-title":"Current perspectives in the immunology of respiratory diseases","author":"Cole, P.J.","unstructured":"Cole PJ. Immunity deficiency and the lung. In: Kay AB, Goetzl EJ, eds. Current perspectives in the immunology of respiratory diseases. Edinburgh: Churchill Livingstone (in press)."},{"key":"ref_8","first-page":"109","article-title":"Antimicrob Chemother 1983;*1","unstructured":"Antimicrob Chemother 1983;*1:109-13."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1016\/S0140-6736(80)91295-7","article-title":"Use of selective media in bacteriological investigation of patients with chronic suppurative respiratory infection","volume":"i:","author":"Roberts, D.E.; Cole, P.J.","year":"1980","unstructured":"Roberts DE, Cole PJ. Use of selective media in bacteriological investigation of patients with chronic suppurative respiratory infection. Lancet 1980;i:796-7.","journal-title":"Lancet"},{"key":"ref_10","first-page":"90","article-title":"Slow growing Haemophilus influenzae in chronic respiratory disease","volume":"i:","author":"Ruhen, R.W.; Karthigasu, K.T.","year":"1981","unstructured":"Ruhen RW, Karthigasu KT. Slow growing Haemophilus influenzae in chronic respiratory disease. MedJ Aust 1981;i:90-1.","journal-title":"MedJ Aust"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1438","DOI":"10.1128\/JB.93.4.1438-1445.1967","article-title":"Induction of L forms of Haemophilus influenzae in culture and their demonstration in human bronchial secretions","volume":"93","author":"Lapinski, E.M.; Flakas, E.D.","year":"1967","unstructured":"Lapinski EM, Flakas ED. Induction of L forms of Haemophilus influenzae in culture and their demonstration in human bronchial secretions. J Bacteriol 1967;93:1438-45.","journal-title":"J Bacteriol"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1016\/S0140-6736(53)90275-X","article-title":"Liquefaction of sputum for bacteriological examination","volume":"ii:","author":"Rawlins, G.A.","year":"1953","unstructured":"Rawlins GA. Liquefaction of sputum for bacteriological examination. Lancet 1953;ii:538.","journal-title":"Lancet"},{"key":"ref_13","first-page":"560","article-title":"Osmotically stable L-forms of Haemophilus influenzae and their significance in testing of sensitivity to penicillins. J7 Clin Pathol","volume":"27","author":"Roberts, D.E.; Ingold, A.; Want, S.V.; May, JR","year":"1974","unstructured":"Roberts DE, Ingold A, Want SV, May JR. Osmotically stable L-forms of Haemophilus influenzae and their significance in testing of sensitivity to penicillins. J7 Clin Pathol 1974;27:560."},{"key":"ref_14","first-page":"703","article-title":"Products released from bacteria are toxic for human ciliary function in vitro","volume":"39","author":"Wilson, R.; Roberts, D.E.; Cole, P.J.","year":"1984","unstructured":"Wilson R, Roberts DE, Cole PJ. Products released from bacteria are toxic for human ciliary function in vitro. 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ScandJ Infect Dis 1978;14 (suppl): 262-6.","journal-title":"ScandJ Infect Dis"},{"key":"ref_16","article-title":"Accepted","year":"1984","unstructured":"(Accepted 7 September 1984)"}],"container-title":["BMJ"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/data.bmj.org\/tdm\/10.1136\/bmj.289.6456.1409","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/bmj.289.6456.1409","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T23:13:46Z","timestamp":1765408426000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.bmj.com\/lookup\/doi\/10.1136\/bmj.289.6456.1409"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1984,11,24]]},"references-count":16,"journal-issue":{"issue":"6456","published-online":{"date-parts":[[1984,11,24]]},"published-print":{"date-parts":[[1984,11,24]]}},"alternative-id":["10.1136\/bmj.289.6456.1409"],"URL":"https:\/\/doi.org\/10.1136\/bmj.289.6456.1409","relation":{},"ISSN":["0007-1447","1468-5833"],"issn-type":[{"value":"0007-1447","type":"print"},{"value":"1468-5833","type":"electronic"}],"subject":[],"published":{"date-parts":[[1984,11,24]]}}}