{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T05:23:58Z","timestamp":1789622638673,"version":"build-2803163510"},"reference-count":25,"publisher":"BMJ","issue":"6678","license":[{"start":{"date-parts":[[1989,4,8]],"date-time":"1989-04-08T00:00:00Z","timestamp":607996800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.bmj.com\/company\/legal-information\/terms-conditions\/legal-information\/tdm-licencepolicy"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BMJ"],"published-print":{"date-parts":[[1989,4,8]]},"abstract":"<jats:p>To determine the survival of cultured allogeneic keratinocytes transplanted to a deep dermal bed 24 tattoos that had been removed by deep shave excision in 19 patients were grafted with sheets of cultured allogeneic keratinocytes from donors of the opposite sex. Cells carrying the Y chromosome were identified in biopsy specimens taken from the graft site by in situ DNA hybridisation with a biotinylated Y probe (pHY 2.1) and visualised with a technique using immunoperoxidase. The cultured allograft sites were biopsied one, two, and three weeks after transplantation. No male cells were identified in any biopsy specimen from female patients who were given transplants of male cultured keratinocytes, and all biopsy specimens from male patients, who received female cultured keratinocytes, showed percentages of male cells within the normal range for male skin. The beneficial effects of cultivated allogeneic keratinocytes result from effects on wound healing other than forming a successful graft that \"takes.\"<\/jats:p>","DOI":"10.1136\/bmj.298.6678.917","type":"journal-article","created":{"date-parts":[[2009,4,2]],"date-time":"2009-04-02T15:19:55Z","timestamp":1238685595000},"page":"917-919","source":"Crossref","is-referenced-by-count":130,"title":["Survival of cultured allogeneic keratinocytes transplanted to deep dermal bed assessed with probe specific for Y chromosome."],"prefix":"10.1136","volume":"298","author":[{"given":"A.","family":"Brain","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Purkis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Coates","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Hackett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Navsaria","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Leigh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[1989,4,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1097\/00007890-197608000-00009","article-title":"Effect of organ culture on the survival of thyroid allografts in mice","volume":"22","author":"Lafferty, K.J.; Bootes, A.; Dart, G.","year":"1976","unstructured":"Lafferty KJ, Bootes A, Dart G, et al. 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Serial cultivation of strains of keratinocytes: the formation of keratinising colonies from single cells. Cell 1975;6:331-43.","journal-title":"Cell"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1111\/1523-1747.ep12497875","article-title":"Cultured human epidermal cells do not synthesise HLA-DR","volume":"78","author":"Morhenn, V.B.; Benike, C.J.; Cox, A.J.; Charron, D.J.; Engelman, E.G.","year":"1982","unstructured":"Morhenn VB, Benike CJ, Cox AJ, Charron DJ, Engelman EG. Cultured human epidermal cells do not synthesise HLA-DR. J Invest Dermatol 1982;78:32-7.","journal-title":"J Invest Dermatol"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1111\/1523-1747.ep12261671","article-title":"Loss of HLA-DR expression by human epidermal cells after growth in culture","volume":"83","author":"Hefton, J.M.; Amberson, J.B.; Biozes, D.G.; Weksler, M.E.","year":"1984","unstructured":"Hefton JM, Amberson JB, Biozes DG, Weksler ME. 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Immunol Rev 1984;80:87-102.","journal-title":"Immunol Rev"},{"key":"ref_12","first-page":"49","article-title":"Characterisation of a human Y repeated sequence and related sequences in higher primates","volume":"87","author":"Cooke, H.J.; Schmidtke, J.; Gosden, JR","year":"1982","unstructured":"Cooke HJ, Schmidtke J, Gosden JR. Characterisation of a human Y repeated sequence and related sequences in higher primates. Chromosoma 1982;87: 49 1-502.","journal-title":"Chromosoma"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1136\/jcp.38.10.1085","article-title":"Sensitive system for visualising biotinylated DNA probes hybridised in situ: rapid sex determination of intact cells","volume":"38","author":"Bums, J.; Chan, V.T.W.; Jonasson, J.A.; Fleming, K.A.; Taylor, S.; JO'D, McGee","year":"1985","unstructured":"Bums J, Chan VTW, Jonasson JA, Fleming KA, Taylor S, McGee JO'D. 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Br J Dermatol 1987;117:591-7.","journal-title":"Br J Dermatol"},{"key":"ref_15","first-page":"1546","article-title":"Cultured human epidermis used as allografts-studies on their differentiation","volume":"87","author":"Mauduit, G.; Faure, M.; Demiderm, A.; Kanitakis, J.; Thivolet, J.","year":"1986","unstructured":"Mauduit G, Faure M, Demiderm A, Kanitakis J, Thivolet J. Cultured human epidermis used as allografts-studies on their differentiation. J7 Invest Dermatol 1986;87:1546."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1159\/000248820","article-title":"Progressive replacement of human cultured epithelial allografts by recipient cells as evidenced by HLA class I antigen expression","volume":"175","author":"Gielen, V.; Faure, M.; Mauduit, G.; Thivolet, J.","year":"1987","unstructured":"Gielen V, Faure M, Mauduit G, Thivolet J. Progressive replacement of human cultured epithelial allografts by recipient cells as evidenced by HLA class I antigen expression. Dermatologica 1987;175:166-70.","journal-title":"Dermatologica"},{"key":"ref_17","first-page":"143","article-title":"Prolonged survival of allogenic cultured keratinocyte sheets in the mouse without imunosuppression","volume":"87","author":"Hammond, E.J.; Ng, R.; Stanley, M.A.","year":"1986","unstructured":"Hammond EJ, Ng R, Stanley MA. Prolonged survival of allogenic cultured keratinocyte sheets in the mouse without imunosuppression. J Invest Dermatol 1986;87:143.","journal-title":"J Invest Dermatol"},{"key":"ref_18","first-page":"59","article-title":"Autologous versus allogenic cultivated epidermis for wound grafting","author":"Aubock, J.; Fritsch, P.","year":"1987","unstructured":"Aubock J, Fritsch P. Autologous versus allogenic cultivated epidermis for wound grafting. In: Teepe R, ed. 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