{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T20:56:24Z","timestamp":1791147384034,"version":"4.1.0"},"reference-count":23,"publisher":"BMJ","issue":"6943","license":[{"start":{"date-parts":[[1994,6,11]],"date-time":"1994-06-11T00:00:00Z","timestamp":771292800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.bmj.com\/company\/legal-information\/terms-conditions\/legal-information\/tdm-licencepolicy"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BMJ"],"accepted":{"date-parts":[[1994,3,28]]},"published-print":{"date-parts":[[1994,6,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:bold>Objective<\/jats:bold>\n                    : To investigate the association of enteroviruses with motor neurone disease, also known as amyotrophic lateral sclerosis.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Design<\/jats:bold>\n                    : Analysis by enterovirus polymerase chain reaction of wax embedded material from spinal cords taken at necropsy from subjects with motor neurone disease and from age and sex matched controls. Setting - Specimens were collected in the west of Scotland and in London between 1982 and 1992.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results<\/jats:bold>\n                    : Sequences specific for a non-poliovirus type enterovirus were detected in spinal cord tissue from subjects with motor neurone disease. Amplification of a 414 base RNA target sequence in the conserved enterovirus 5' untranslated region from wax embedded tissue sections was successful in tissue from eight of 11 cases of sporadic motor neurone disease, one of two cases of familial motor neurone disease, and the one case of poliomyelitis, but not in the six matched controls or one case of antecedent poliomyelitis. In addition, sequences were detected in spinal cords from one monkey infected with wild type poliovirus and one monkey infected with polio vaccine. Comparison of sequences from cases of motor neurone disease with sequences of corresponding regions of the 5' untranslated regions of known picornaviruses showed them to be tightly grouped within the enterovirus genus closely related to coxsackievirus type B but not to polioviruses. Sequences derived from different parts of the spinal cord of the same subjects were identical, but sequences differed between individual subjects.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusions<\/jats:bold>\n                    : Conserved enteroviral sequences closely related to coxsackie B virus sequences were detectable in spinal cords from subjects with sporadic motor neurone disease and from one subject with possible familial motor neurone disease.\n                  <\/jats:p>","DOI":"10.1136\/bmj.308.6943.1541","type":"journal-article","created":{"date-parts":[[2011,10,27]],"date-time":"2011-10-27T19:22:52Z","timestamp":1319743372000},"page":"1541-1543","source":"Crossref","is-referenced-by-count":67,"title":["Sequences specific for enterovirus detected in spinal cord from patients with motor neurone disease"],"prefix":"10.1136","volume":"308","author":[{"given":"C J","family":"Woodall","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M H","family":"Riding","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D I","family":"Graham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G B","family":"Clements","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[1994,6,11]]},"reference":[{"key":"2025122310405950000_308.6943.1541.1","first-page":"324","volume-title":"Topley and Wilson's principles of bacteriology, virology and immunity","volume":"4","author":"Minor","year":"1990"},{"key":"2025122310405950000_308.6943.1541.2","unstructured":"Heiman-Patterson TD Gudesblatt MJ Tahmoush AJ .In: Caroscio JT ed.Amyotrophic lateral sclerosis - a guide to patient care.New York: Thieme Medical Publishers,1986: 85\u2013102."},{"key":"2025122310405950000_308.6943.1541.3","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(90)92492-Z"},{"key":"2025122310405950000_308.6943.1541.4","first-page":"1028","article-title":"Discussion on motor neurone disease","volume":"55","year":"1962","journal-title":"Proceedings of Royal Society of Medicine"},{"key":"2025122310405950000_308.6943.1541.5","unstructured":"Poskanzer DC Cantor HM Kaplan GS .In: Norris FH Kurland LT eds.Motor neurone diseases: research on amyotrophic lateral sclerosis and related disorders. Norris FH Kurland LT , eds. Philadelphia: Grune and Stratton Inc,1969: 286\u20138."},{"key":"2025122310405950000_308.6943.1541.6","doi-asserted-by":"publisher","DOI":"10.1001\/archneur.1980.00500540090016"},{"key":"2025122310405950000_308.6943.1541.7","first-page":"1319","article-title":"Motorneuron disease and past poliomyelitis in England and Wales","volume":"i","author":"Martyn","year":"1989","journal-title":"Lancet"},{"key":"2025122310405950000_308.6943.1541.8","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1002\/ana.410050419","article-title":"Failure to detect poliovirus genetic information in amyotrophic lateral sclerosis","volume":"5","author":"Viola","year":"1980","journal-title":"Ann Neurol"},{"key":"2025122310405950000_308.6943.1541.9","doi-asserted-by":"crossref","first-page":"1319","DOI":"10.1212\/WNL.30.12.1319","article-title":"Attempts to demonstrate virus in amyotrophic lateral sclerosis","volume":"30","author":"Weiner","year":"1980","journal-title":"Neurology"},{"key":"2025122310405950000_308.6943.1541.10","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410180311"},{"key":"2025122310405950000_308.6943.1541.11","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1093\/infdis\/159.3.384","article-title":"Viruses can cause disease in the absence of morphological evidence of cell injury: implication for uncovering new diseases in the future","volume":"139","author":"Oldstone","year":"1989","journal-title":"J Infect Dis"},{"key":"2025122310405950000_308.6943.1541.12","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.46.3.276"},{"key":"2025122310405950000_308.6943.1541.13","doi-asserted-by":"crossref","first-page":"692","DOI":"10.1136\/bmj.302.6778.692","article-title":"Enteroviral RNA sequences detected by polymerase chain reaction in muscle of patients with post viral fatigue syndrome","volume":"302","author":"Gow","year":"1991","journal-title":"BMJ"},{"key":"2025122310405950000_308.6943.1541.14","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.15.5698"},{"key":"2025122310405950000_308.6943.1541.15","doi-asserted-by":"crossref","unstructured":"Wright DK Manos MM .PCR protocols: a guide to methods and applications.London: Academic Press,1990: 153\u20138.","DOI":"10.1016\/B978-0-12-372180-8.50023-8"},{"key":"2025122310405950000_308.6943.1541.16","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ge.22.120188.002513"},{"key":"2025122310405950000_308.6943.1541.17","unstructured":"Genetics Computer Group .Program manual for the GCG package, version 7.Madison, WI: GCG,1991."},{"key":"2025122310405950000_308.6943.1541.18","unstructured":"Rueckert RR .In: Fields BN Knipe DM Chanock RM Hirsch MS Melnick JL Monath TP eds.Fields virology.New York: Raven Press,1990: 507\u201348."},{"key":"2025122310405950000_308.6943.1541.19","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-71-11-2483"},{"key":"2025122310405950000_308.6943.1541.20","unstructured":"Palmenberg AC .In: Semler B Ehrenfeld E eds.Molecular aspects of picornavirus infection and detection.Washington DC: ASM Publications,1988: 211\u201341."},{"key":"2025122310405950000_308.6943.1541.21","doi-asserted-by":"publisher","DOI":"10.1038\/362059a0"},{"key":"2025122310405950000_308.6943.1541.22","doi-asserted-by":"publisher","DOI":"10.1126\/science.8351519"},{"key":"2025122310405950000_308.6943.1541.23","unstructured":"Norby E .Oxman MN. In: Fields BN Knipe DM Chanock RM Hirsch MS Melnick JL Monath TP eds.Virology.New York: Raven Press,1990: 1028\u201332."}],"container-title":["BMJ"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/data.bmj.org\/tdm\/10.1136\/bmj.308.6943.1541","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/bmj.308.6943.1541","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T18:41:12Z","timestamp":1766515272000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.bmj.com\/lookup\/doi\/10.1136\/bmj.308.6943.1541"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,6,11]]},"references-count":23,"journal-issue":{"issue":"6943","published-online":{"date-parts":[[1994,6,11]]},"published-print":{"date-parts":[[1994,6,11]]}},"alternative-id":["10.1136\/bmj.308.6943.1541"],"URL":"https:\/\/doi.org\/10.1136\/bmj.308.6943.1541","relation":{},"ISSN":["0959-8138","1468-5833"],"issn-type":[{"value":"0959-8138","type":"print"},{"value":"1468-5833","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,6,11]]}}}