{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T23:27:36Z","timestamp":1778110056275,"version":"3.51.4"},"reference-count":44,"publisher":"BMJ","issue":"1","content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["BMJ Open Diab Res Care"],"accepted":{"date-parts":[[2015,6,26]]},"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>\n                     <jats:italic>CD300LG<\/jats:italic> rs72836561 (c.313C&gt;T, p.Arg82Cys) has in genetic-epidemiological studies been associated with the lipoprotein abnormalities of the metabolic syndrome. <jats:italic>CD300LG<\/jats:italic> belongs to the CD300-family of membrane-bound molecules which have the ability to recognize and interact with extracellular lipids. We tested whether this specific polymorphism results in abnormal lipid accumulation in skeletal muscle and liver and other indices of metabolic dysfunction.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>40 healthy men with a mean age of 55\u2005years were characterized metabolically including assessment of insulin sensitivity by the hyperinsulinemic euglycemic clamp, intrahepatic lipid content (IHLC) and intramyocellular lipid content (IMCL) by MR spectroscopy, and \u03b2-cell function by an intravenous glucose tolerance test. Changes in insulin signaling and <jats:italic>CD300LG<\/jats:italic> mRNA expression were determined by western blotting and quantitative PCR in muscle and adipose tissue.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Compared with the 20 controls (CC carriers), the 20 CT carriers (polymorphism carriers) had higher IMCL (p=0.045), a reduced fasting forearm glucose uptake (p=0.011), a trend toward lower M-values during the clamp; 6.0\u2005mg\/kg\/min vs 7.1 (p=0.10), and higher IHLC (p=0.10). CT carriers had lower <jats:italic>CD300LG<\/jats:italic> mRNA expression and <jats:italic>CD300LG<\/jats:italic> expression in muscle correlated with IMCL (\u03b2=\u22120.35, p=0.046), forearm glucose uptake (\u03b2=0.37, p=0.03), and tended to correlate with the M-value (\u03b2=0.33, p=0.06), independently of <jats:italic>CD300LG<\/jats:italic> genotype. \u03b2-cell function was unaffected.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>The <jats:italic>CD300LG<\/jats:italic> polymorphism was associated with decreased <jats:italic>CD300LG<\/jats:italic> mRNA expression in muscle and adipose tissue, increased IMCL, and abnormalities of glucose metabolism. <jats:italic>CD300LG<\/jats:italic> mRNA levels correlated with IMCL and forearm glucose uptake. These findings link a specific <jats:italic>CD300LG<\/jats:italic> polymorphism with features of the metabolic syndrome suggesting a role for <jats:italic>CD300LG<\/jats:italic> in the regulation of common metabolic traits.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Trial registration number<\/jats:title>\n                  <jats:p>NCT01571609.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1136\/bmjdrc-2015-000095","type":"journal-article","created":{"date-parts":[[2016,1,22]],"date-time":"2016-01-22T15:44:47Z","timestamp":1453477487000},"page":"e000095","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":20,"title":["Reduced <i>CD300LG<\/i> mRNA tissue expression, increased intramyocellular lipid content and impaired glucose metabolism in healthy male carriers of Arg82Cys in <i>CD300LG<\/i>: a novel genometabolic cross-link between <i>CD300LG<\/i> and common metabolic phenotypes"],"prefix":"10.1136","volume":"3","clinical-trial-number":[{"clinical-trial-number":"nct01571609","registry":"10.18810\/clinical-trials-gov"}],"author":[{"given":"Julie","family":"St\u00f8y","sequence":"first","affiliation":[{"name":"Department of Internal Medicine and Endocrinology, Aarhus University Hospital, Aarhus, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulla","family":"Kampmann","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Endocrinology, Aarhus University Hospital, Aarhus, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annette","family":"Mengel","sequence":"additional","affiliation":[{"name":"Medical Research Laboratories, Institute for Clinical Medicine, Aarhus University, Aarhus, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nils E","family":"Magnusson","sequence":"additional","affiliation":[{"name":"Medical Research Laboratories, Institute for 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Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans","family":"St\u00f8dkilde-J\u00f8rgensen","sequence":"additional","affiliation":[{"name":"The MR Research Center, Aarhus University Hospital, Aarhus, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivan","family":"Brandslund","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry, Vejle Hospital, Vejle, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cramer","family":"Christensen","sequence":"additional","affiliation":[{"name":"Department of Medicine, Vejle Hospital, Vejle, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Torben","family":"Hansen","sequence":"additional","affiliation":[{"name":"Faculty of Health and Medical Sciences, The Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, Copenhagen, 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