{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T16:14:07Z","timestamp":1759335247008,"version":"3.41.2"},"reference-count":63,"publisher":"BMJ","issue":"1","funder":[{"name":"JSPS Grant-in-Aid for Scientific Research B"},{"name":"JSPS Grant-in-Aid for Young Scientists"},{"name":"MEXT-Supported Program for the Strategic Research Foundation at Private Universities"},{"name":"Princess Takamatsu Cancer Research grants"},{"DOI":"10.13039\/100007449","name":"Takeda Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007449","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the Medical School Faculty and Alumni Grant from Keio University Medical Science Fund"}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["BMJ Open Gastroenterol"],"accepted":{"date-parts":[[2017,7,7]]},"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:sec>\n                  <jats:title>Objective<\/jats:title>\n                  <jats:p>Circulating microRNAs (miRNAs) are promising biomarkers for the early detection of cancers. This study aimed to address potential circulating miRNAs to monitor the progression from Barrett\u2019s oesophagus (BO) to oesophageal adenocarcinoma (OAC).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Design<\/jats:title>\n                  <jats:p>We comprehensively analysed tissue and serum miRNA expression profiles of BO mice model (L2-interleukin-1\u03b2 (IL-1\u03b2) mice) using microarray analysis. To validate the data from mice, a published dataset of human plasma miRNAs, consisting of eight patients with OAC, eight with BO and six healthy controls, was used (GSE51410).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>We identified 20 upregulated miRNAs and 44 downregulated miRNAs both in tissues and in sera of 46-week-old mice compared with 28-week-old mice. Two of the 20 miRNAs (miR-128-3\u2009p and miR-328-3\u2009p) were upregulated, and five of the 44 miRNAs (miR-143-3\u2009p, miR-144-3\u2009p, miR-15a-5p, miR-1-3\u2009p and miR-133b) were downregulated in plasma of patients with OAC compared with plasma of patients with BO. Receiver operating characteristic curve analysis revealed that a prediction index calculated by the above-mentioned seven miRNAs could discriminate between patients with OAC and those without OAC with the area under the curve of 0.91, sensitivity of 1 and specificity of 0.75.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Levels of the seven circulating miRNAs may represent the tissue miRNA levels and could be promising non-invasive biomarkers to evaluate the carcinogenic process of BO.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1136\/bmjgast-2017-000160","type":"journal-article","created":{"date-parts":[[2017,9,9]],"date-time":"2017-09-09T20:10:16Z","timestamp":1504987816000},"page":"e000160","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":13,"title":["Circulating microRNAs as potential biomarkers to detect 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