{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T22:15:17Z","timestamp":1759011317596},"reference-count":20,"publisher":"BMJ","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BMJ Open"],"accepted":{"date-parts":[[2012,10,29]]},"published-print":{"date-parts":[[2012]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>The prevalence of active tuberculosis (TB) is low in Finland, but outbreaks do occur. Following exposure national guidelines recommend either tuberculosis skin test or interferon-\u03b3-release assay-testing of asymptomatic children. The aim of this study was to compare QuantiFERON-TB Gold In-Tube test (QFT) and interferon-\u03b3-inducible protein (IP-10) release assay for detection of<jats:italic>Mycobacterium tuberculosis<\/jats:italic>infection following exposure to TB in a primary school.<\/jats:p><\/jats:sec><jats:sec><jats:title>Design<\/jats:title><jats:p>A prospective cohort study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Setting<\/jats:title><jats:p>School children in Helsinki, Finland.<\/jats:p><\/jats:sec><jats:sec><jats:title>Participants<\/jats:title><jats:p>Two siblings of the index case and 58 classmates exposed to<jats:italic>M tuberculosis<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Intervention<\/jats:title><jats:p>All the children were screened using the QFT, which was used to guide preventive treatment. All those exposed were followed up through the national TB registry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Outcome measures<\/jats:title><jats:p>IP-10 was measured in plasma supernatants from the QFT test supernatants and in plasma dried and stored for 1\u2005year on filter paper. IP-10 test results were calculated using preset algorithms for positive and indeterminate tests. The negative predictive values of the tests were assessed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>At an initial screening 2\u2005months after the debut of symptoms in the index case, QFT was positive in two children; 56 tests were negative; one was indeterminate and one was borderline. IP-10 showed a perfect concordance between the dried plasma spot and plasma method; two children were IP-10 positive and two were IP-10 indeterminate. There were two (3%) discordant results between the QFT and IP-10 tests. Four children converted to positive QFT at a 1\u20133\u2005month follow-up. None of the QFT negative\/borderline children developed TB in the 4-year period since exposure.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We demonstrated that IP-10 and QFT perform comparably as screening tools for infection with<jats:italic>M tuberculosis<\/jats:italic>in a contact investigation. IP-10 determined in dried plasma spots was at par with IP-10 determined in plasma, which further supports the usefulness of this alternative approach.<\/jats:p><\/jats:sec>","DOI":"10.1136\/bmjopen-2012-001751","type":"journal-article","created":{"date-parts":[[2012,12,6]],"date-time":"2012-12-06T02:00:29Z","timestamp":1354759229000},"page":"e001751","source":"Crossref","is-referenced-by-count":8,"title":["Evaluation of the filter paper IP-10 tests in school children after exposure to tuberculosis: a prospective cohort study with a 4-year follow-up"],"prefix":"10.1136","volume":"2","author":[{"given":"Tamara","family":"Tuuminen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eeva","family":"Salo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hannele","family":"Kotilainen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Morten","family":"Ruhwald","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[2012,12,4]]},"reference":[{"key":"2024051504020186000_2.6.e001751.1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jis008"},{"key":"2024051504020186000_2.6.e001751.2","first-page":"392","article-title":"The natural history of childhood intra-thoracic tuberculosis: a critical review of literature from the pre-chemotherapy era State of the Art","volume":"8","author":"Marais","year":"2004","journal-title":"Int J Tuberc Lung Dis"},{"key":"2024051504020186000_2.6.e001751.3","doi-asserted-by":"publisher","DOI":"10.1586\/erm.11.97"},{"key":"2024051504020186000_2.6.e001751.4","doi-asserted-by":"crossref","first-page":"1089","DOI":"10.1097\/INF.0b013e3182611152","article-title":"Interferon-gamma-induced-protein-10 concentrations in children with previous tuberculosis infections and disease","volume":"31","author":"Bihari","year":"2012","journal-title":"Pediatr Infect Dis J"},{"key":"2024051504020186000_2.6.e001751.5","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0023733"},{"key":"2024051504020186000_2.6.e001751.6","unstructured":"Hulkko T Lyytikainen O Jaakola S . Infectious diseases in Finland 2010. National Institute for Health and Welfare (THL) www.thl.fi\/thl-client\/pdfs\/c22be7c1-5e9a-4b2d-a7f8-7be4c530ffe5 (accessed 7 Jun 2012)."},{"key":"2024051504020186000_2.6.e001751.7","doi-asserted-by":"publisher","DOI":"10.1186\/1756-0500-2-9"},{"key":"2024051504020186000_2.6.e001751.8","doi-asserted-by":"crossref","first-page":"381929","DOI":"10.1155\/2012\/381929","article-title":"Immunosuppression adversely affects TST but not IGRAs in patients with psoriasis or inflammatory musculoskeletal diseases","volume":"2012","author":"Tavast","year":"2012","journal-title":"Int J Rheumatol"},{"key":"2024051504020186000_2.6.e001751.9","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0039228"},{"key":"2024051504020186000_2.6.e001751.10","doi-asserted-by":"crossref","unstructured":"Aabye MG Latorre I Diaz j . Dried plasma spots in the diagnosis of TB: IP-10 release assay on filter paper. Eur Respir J 2012;in press.","DOI":"10.1183\/09031936.00129412"},{"key":"2024051504020186000_2.6.e001751.11","doi-asserted-by":"publisher","DOI":"10.1016\/j.tube.2011.01.001"},{"key":"2024051504020186000_2.6.e001751.12","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00055508"},{"key":"2024051504020186000_2.6.e001751.13","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1016\/j.tube.2011.02.006","article-title":"Discriminating between latent and active tuberculosis with multiple biomarker responses","volume":"91","author":"Frahm","year":"2011","journal-title":"Tuberculosis (Edinberg)"},{"key":"2024051504020186000_2.6.e001751.14","first-page":"731","article-title":"Chemokine IP-10: an adjunct marker for latent tuberculosis infection in children","volume":"13","author":"Lighter","year":"2009","journal-title":"Int J Tuberc Lung Dis"},{"key":"2024051504020186000_2.6.e001751.15","doi-asserted-by":"publisher","DOI":"10.3109\/00365548.2011.632644"},{"key":"2024051504020186000_2.6.e001751.16","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0008517"},{"key":"2024051504020186000_2.6.e001751.17","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00320-09"},{"key":"2024051504020186000_2.6.e001751.18","unstructured":"Cellestis website. 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