{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T12:23:13Z","timestamp":1781871793382,"version":"3.54.5"},"reference-count":23,"publisher":"BMJ","issue":"8","content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["BMJ Open"],"accepted":{"date-parts":[[2016,7,4]]},"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      To determine the antimicrobial susceptibility and genotype distribution of\n                      <jats:italic>Neisseria gonorrhoeae<\/jats:italic>\n                      strains isolated from a cohort of patients in Nuuk, Greenland in order to assess the risk of rapid spread in the event of introduction of new strains.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Gonococcal isolates (n=102) obtained from a prospective cohort study of ciprofloxacin resistance were collected between March 2012 and February 2013. Etest minimal inhibitory concentrations (MICs) were determined for ciprofloxacin, azithromycin, ceftriaxone, penicillin, tetracycline, spectinomycin and gentamicin. All isolates were subjected to molecular typing using\n                      <jats:italic>N. gonorrhoeae<\/jats:italic>\n                      multiantigen sequence typing (NG-MAST). After the introduction of a ciprofloxacin-resistant strain in early 2014, an additional 18 isolates were characterised.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>During the study period, all 102 isolates were fully susceptible to ciprofloxacin (\u22640.03\u2005mg\/L), azithromycin, spectinomycin, gentamicin and ceftriaxone. 10 different NG-MAST types circulated in Nuuk but 7 were found as single isolates, and 3 of the 7 belonged to 1 of the 3 major genogroups (G210, G9816 and G9817) together comprising 96% of the 102 isolates. ST210 accounted for 55% of the 102 strains. The newly introduced ciprofloxacin resistant strain belonged to ST2400 and dominated the population with 59% resistant strains within 6\u2005months after its introduction. All G2400 strains had MICs\u22652\u2005mg\/L.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Introduction of a ciprofloxacin-resistant strain into a very homogeneous\n                      <jats:italic>N. gonorrhoeae<\/jats:italic>\n                      population led to an explosive spread of the resistant clone, probably as a result of large sexual networks suggested by the strain homogeneity. Careful surveillance of antimicrobial susceptibility is essential to avoid widespread treatment failure in closed populations.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/bmjopen-2016-011998","type":"journal-article","created":{"date-parts":[[2016,8,30]],"date-time":"2016-08-30T21:54:18Z","timestamp":1472594058000},"page":"e011998","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":6,"title":["Rapid spread of\n                    <i>Neisseria gonorrhoeae<\/i>\n                    ciprofloxacin resistance due to a newly introduced resistant strain in Nuuk, Greenland, 2012\u20132015: a community-based prospective cohort study"],"prefix":"10.1136","volume":"6","author":[{"given":"Michael Lynge","family":"Pedersen","sequence":"first","affiliation":[{"name":"Greenland Center for Health Research, Institute of Nursing and Health Science, University of Greenland, Nuuk, Greenland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Poulsen","sequence":"additional","affiliation":[{"name":"Queen Ingrid Hospital, Nuuk, Greenland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lene","family":"Berthelsen","sequence":"additional","affiliation":[{"name":"Microbiology and Infection Control, Statens Serum Institut, Copenhagen S, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"N\u00f8rgaard","sequence":"additional","affiliation":[{"name":"Microbiology and Infection Control, Statens Serum Institut, Copenhagen S, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steen","family":"Hoffmann","sequence":"additional","affiliation":[{"name":"Microbiology and Infection Control, Statens Serum Institut, Copenhagen S, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f8rgen Skov","family":"Jensen","sequence":"additional","affiliation":[{"name":"Microbiology and Infection Control, Statens Serum Institut, Copenhagen S, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[2016,8,30]]},"reference":[{"key":"2025121603215304000_6.8.e011998.1","first-page":"65","article-title":"Some aspects of venereal diseases in Greenland","volume":"56","author":"From","year":"1980","journal-title":"Br J Vener Dis"},{"key":"2025121603215304000_6.8.e011998.2","first-page":"327","article-title":"Prevalence of Chlamydia trachomatis and Neisseria gonorrhoeae infections in Greenland. 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