{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T04:54:52Z","timestamp":1776228892736,"version":"3.50.1"},"reference-count":26,"publisher":"BMJ","issue":"1","content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["BMJ Open"],"accepted":{"date-parts":[[2016,12,20]]},"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\u03b2-Thalassaemia is widely found in Southwestern China. Characterisation of \u03b2-thalassaemia can improve screening and prenatal diagnosis for at-risk populations.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Design<\/jats:title>\n                    <jats:p>A retrospective study.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      In this study, the levels of haemoglobin alpha 2 (HbA\n                      <jats:sub>2<\/jats:sub>\n                      ) and haemoglobin alpha (HbA) were analysed by gender for a total of 15\u2005067 subjects screened by capillary electrophoresis. The cut-off value with the highest accuracy was established to identify \u03b2-thalassaemia in 723 patients suspected to have this disease. Haematological and electrophoretic characterisation of eight common types of \u03b2-thalassaemia were analysed in 486 \u03b2-thalassaemia subjects.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      HbA levels were significantly higher in men than in women, but there was no significant difference on HbA\n                      <jats:sub>2<\/jats:sub>\n                      levels. A new cut-off value for the diagnosis of \u03b2-thalassaemia (HbA\n                      <jats:sub>2<\/jats:sub>\n                      \u22654.0%) with the highest accuracy was proposed for the studied populations. Haemoglobin (Hb) was significantly higher in men compared with women (p&lt;0.05), whereas no statistically significant differences were found for mean cell volume (MCV), mean cell haemoglobin (MCH), HbA and HbA\n                      <jats:sub>2<\/jats:sub>\n                      . The haemoglobin E (HbE) group showed comparatively higher values for haematological indices (Hb, MCV and MCH) than the other genotypes in heterozygous \u03b2-thalassaemia groups (p&lt;0.05), and \u221228 (A&gt;G) (HBB (\u03b2-globin):c.\u221278A&gt;C) had signi\ufb01cantly higher HbA\n                      <jats:sub>2<\/jats:sub>\n                      values compared with other \u03b2-thalassaemia.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Ethnic groups have diversified \u03b2-globin gene mutations and considerable haematological variations. Our study will lay the foundation for screening programmes and clinical management of thalassaemia in Southwestern China.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/bmjopen-2016-013367","type":"journal-article","created":{"date-parts":[[2017,1,31]],"date-time":"2017-01-31T20:10:38Z","timestamp":1485893438000},"page":"e013367","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":16,"title":["Haematological and electrophoretic characterisation of \u03b2-thalassaemia in Yunnan province of Southwestern China"],"prefix":"10.1136","volume":"7","author":[{"given":"Jie","family":"Zhang","sequence":"first","affiliation":[{"name":"Yunnan Provincial Key Laboratory For Birth Defects and Genetic Diseases, Genetic Diagnosis Center, the First People's Hospital of Yunnan Province, Kunming, China"},{"name":"Genetics Department, Kunming University of Science and 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