{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T05:20:41Z","timestamp":1790486441972,"version":"4.1.0"},"reference-count":58,"publisher":"BMJ","issue":"11","funder":[{"name":"Ph.D Grant (CIFRE) Laboratoires ANIOS"}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["BMJ Open"],"accepted":{"date-parts":[[2017,9,28]]},"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Monitoring fungal aerocontamination is an essential measure to prevent severe invasive aspergillosis (IA) infections in hospitals. One central block among 32 blocks of Edouard Herriot Hospital (EHH) was entirely demolished in 2015, while care activities continued in surrounding blocks. The main objective was to undertake broad environmental monitoring and clinical surveillance of IA cases to document fungal dispersion during major deconstruction work and to assess clinical risk.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and analysis<\/jats:title>\n                    <jats:p>\n                      A daily environmental survey of fungal loads was conducted in eight wards located near the demolition site. Air was collected inside and outside selected wards by agar impact samplers. Daily spore concentrations were monitored continuously by volumetric samplers at a flow rate of 10\u2009L.min\n                      <jats:sup>-1<\/jats:sup>\n                      . Daily temperature, wind direction and speed as well as relative humidity were recorded by the French meteorological station Meteociel.\n                      <jats:italic>Aspergillus fumigatus<\/jats:italic>\n                      strains stored will be genotyped by multiple-locus, variable-number, tandem-repeat analysis. Antifungal susceptibility will be assessed by E-test strips on Roswell Park Memorial Institute medium supplemented with agar. Ascertaining the adequacy of current environmental monitoring techniques in hospital is of growing importance, considering the rising impact of fungal infections and of curative antifungal costs. The present study could improve the daily management of IA risk during major deconstruction work and generate new data to ameliorate and redefine current guidelines.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Ethics and dissemination<\/jats:title>\n                    <jats:p>This study was approved by the clinical research and ethics committees of EHH.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/bmjopen-2017-018109","type":"journal-article","created":{"date-parts":[[2017,11,25]],"date-time":"2017-11-25T04:40:26Z","timestamp":1511584826000},"page":"e018109","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":13,"title":["Monitoring of clinical strains and environmental fungal aerocontamination to prevent invasive aspergillosis infections in hospital during large deconstruction work: a protocol study"],"prefix":"10.1136","volume":"7","author":[{"given":"Sophie Tiphaine","family":"Loeffert","sequence":"first","affiliation":[{"name":"Laboratoire des Pathog\u00e8nes Emergents-Fondation M\u00e9rieux, Centre International de Recherche en Infectiologie (CIRI), Universit\u00e9 de Lyon, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elise","family":"Melloul","sequence":"additional","affiliation":[{"name":"EA 7380 Dynamyc, EnvA, UPEC, Universit\u00e9 Paris Est, Cr\u00e9teil, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9dric","family":"Dananch\u00e9","sequence":"additional","affiliation":[{"name":"Laboratoire des Pathog\u00e8nes Emergents-Fondation M\u00e9rieux, Centre International de Recherche en Infectiologie (CIRI), Universit\u00e9 de Lyon, Lyon, France"},{"name":"Unit\u00e9 d\u2019hygi\u00e8ne, \u00e9pid\u00e9miologie et pr\u00e9vention, Groupement Hospitalier Centre, Hospices Civils de Lyon, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laetitia","family":"H\u00e9naff","sequence":"additional","affiliation":[{"name":"Laboratoire des Pathog\u00e8nes Emergents-Fondation M\u00e9rieux, Centre International de Recherche en Infectiologie (CIRI), Universit\u00e9 de Lyon, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"B\u00e9net","sequence":"additional","affiliation":[{"name":"Laboratoire des Pathog\u00e8nes Emergents-Fondation M\u00e9rieux, Centre International de Recherche en Infectiologie (CIRI), Universit\u00e9 de Lyon, Lyon, France"},{"name":"Unit\u00e9 d\u2019hygi\u00e8ne, \u00e9pid\u00e9miologie et pr\u00e9vention, Groupement Hospitalier Centre, Hospices Civils de Lyon, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Cassier","sequence":"additional","affiliation":[{"name":"Laboratoire de Biologie S\u00e9curit\u00e9 Environnement, Groupement Hospitalier Centre, Hospices Civils de Lyon, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Damien","family":"Dupont","sequence":"additional","affiliation":[{"name":"Institut de Parasitologie et de Mycologie M\u00e9dicale, H\u00f4pital de la Croix Rousse, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques","family":"Guillot","sequence":"additional","affiliation":[{"name":"EA 7380 Dynamyc, EnvA, UPEC, Universit\u00e9 Paris Est, Cr\u00e9teil, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7oise","family":"Botterel","sequence":"additional","affiliation":[{"name":"EA 7380 Dynamyc, EnvA, UPEC, Universit\u00e9 Paris Est, Cr\u00e9teil, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martine","family":"Wallon","sequence":"additional","affiliation":[{"name":"Institut de Parasitologie et de Mycologie M\u00e9dicale, H\u00f4pital de la Croix Rousse, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Paule","family":"Gustin","sequence":"additional","affiliation":[{"name":"Laboratoire des Pathog\u00e8nes Emergents-Fondation M\u00e9rieux, Centre International de Recherche en Infectiologie (CIRI), Universit\u00e9 de Lyon, Lyon, France"},{"name":"D\u00e9partement de sant\u00e9 Publique, Institut des Sciences Pharmaceutiques et Biologiques (ISPB)-Facult\u00e9 de Pharmacie, Universit\u00e9 de Lyon, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Vanhems","sequence":"additional","affiliation":[{"name":"Laboratoire des Pathog\u00e8nes Emergents-Fondation M\u00e9rieux, Centre International de Recherche en Infectiologie (CIRI), Universit\u00e9 de Lyon, Lyon, France"},{"name":"Unit\u00e9 d\u2019hygi\u00e8ne, \u00e9pid\u00e9miologie et pr\u00e9vention, Groupement Hospitalier Centre, Hospices Civils de Lyon, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[2017,11,25]]},"reference":[{"key":"2025121601050692000_7.11.e018109.1","doi-asserted-by":"publisher","DOI":"10.1086\/513943"},{"key":"2025121601050692000_7.11.e018109.2","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5520(02)00035-1"},{"key":"2025121601050692000_7.11.e018109.3","doi-asserted-by":"publisher","DOI":"10.1086\/591969"},{"key":"2025121601050692000_7.11.e018109.4","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2011.044636"},{"key":"2025121601050692000_7.11.e018109.5","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.2009.01710.x"},{"key":"2025121601050692000_7.11.e018109.6","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.13735"},{"key":"2025121601050692000_7.11.e018109.7","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2011.03548.x"},{"key":"2025121601050692000_7.11.e018109.8","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkv177"},{"key":"2025121601050692000_7.11.e018109.9","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003633"},{"key":"2025121601050692000_7.11.e018109.10","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0000000000000005"},{"key":"2025121601050692000_7.11.e018109.11","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00127-15"},{"key":"2025121601050692000_7.11.e018109.12","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myv029"},{"key":"2025121601050692000_7.11.e018109.13","doi-asserted-by":"publisher","DOI":"10.1016\/j.fbr.2011.07.002"},{"key":"2025121601050692000_7.11.e018109.14","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.mycmed.2012.01.003","article-title":"[Quantitative assessment of fungal risk in the case of construction works in healthcare establishments: Proposed indicators for the determination of the impact of management precautions on the risk of fungal infection]","volume":"22","author":"Gangneux","year":"2012","journal-title":"J Mycol Med"},{"key":"2025121601050692000_7.11.e018109.15","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/civ297"},{"key":"2025121601050692000_7.11.e018109.16","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268800067893"},{"key":"2025121601050692000_7.11.e018109.17","doi-asserted-by":"publisher","DOI":"10.1086\/521378"},{"key":"2025121601050692000_7.11.e018109.18","doi-asserted-by":"publisher","DOI":"10.3109\/1040841X.2013.777687"},{"key":"2025121601050692000_7.11.e018109.19","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2014.02.008"},{"key":"2025121601050692000_7.11.e018109.20","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajic.2009.07.011"},{"key":"2025121601050692000_7.11.e018109.21","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0177263","article-title":"Evaluation of hirst-type spore trap to monitor environmental fungal load in hospital","volume":"12","author":"Dananch\u00e9","year":"2017","journal-title":"PLoS One"},{"key":"2025121601050692000_7.11.e018109.22","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/j.mycmed.2006.10.002","article-title":"Control of fungal environmental risk in French Hospitals","volume":"16","author":"Gangneux","year":"2006","journal-title":"Journal de Mycologie M\u00e9dicale"},{"key":"2025121601050692000_7.11.e018109.23","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1080\/15459624.2013.800955","article-title":"Comparative evaluation of three impactor samplers for measuring airborne bacteria and fungi concentrations","volume":"10","author":"M\u00e9heust","year":"2013","journal-title":"J Occup Environ Hyg"},{"key":"2025121601050692000_7.11.e018109.24","doi-asserted-by":"crossref","first-page":"1016","DOI":"10.1016\/j.ajic.2012.01.029","article-title":"Technical note: the effect of different incubation temperatures on the recovery of Aspergillus species from hospital air","volume":"40","author":"Boff","year":"2012","journal-title":"Am J Infect Control"},{"key":"2025121601050692000_7.11.e018109.25","doi-asserted-by":"publisher","DOI":"10.1086\/588660"},{"key":"2025121601050692000_7.11.e018109.26","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(05)70238-3"},{"key":"2025121601050692000_7.11.e018109.27","doi-asserted-by":"crossref","first-page":"24","DOI":"10.3109\/13693786.2010.509335","article-title":"Nosocomial or community-acquired?","volume":"49","author":"Nicolle","year":"2011","journal-title":"Med Mycol"},{"key":"2025121601050692000_7.11.e018109.28","doi-asserted-by":"publisher","DOI":"10.3109\/13693786.2012.687462"},{"key":"2025121601050692000_7.11.e018109.29","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-10-315"},{"key":"2025121601050692000_7.11.e018109.30","doi-asserted-by":"crossref","DOI":"10.1186\/1471-2105-13-87","article-title":"PHYLOViZ: phylogenetic inference and data visualization for sequence based typing methods","volume":"13","author":"Francisco","year":"2012","journal-title":"BMC Bioinformatics"},{"key":"2025121601050692000_7.11.e018109.31","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01792-16"},{"key":"2025121601050692000_7.11.e018109.32","first-page":"1","article-title":"Identification du risque et mise en place de mesures de gestion","volume":"19","author":"Risque infectieux fongique et travaux en \u00e9tablissements de sant\u00e9","year":"2009","journal-title":"Hygi\u00e8ne"},{"key":"2025121601050692000_7.11.e018109.33","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.funeco.2013.11.004","article-title":"Outdoor airborne fungi captured by viable and non-viable methods","volume":"7","author":"Fern\u00e1ndez-Rodr\u00edguez","year":"2014","journal-title":"Fungal Ecol"},{"key":"2025121601050692000_7.11.e018109.34","doi-asserted-by":"publisher","DOI":"10.1007\/s10453-010-9177-8"},{"key":"2025121601050692000_7.11.e018109.35","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.funeco.2013.11.004","article-title":"Outdoor airborne fungi captured by viable and non-viable methods","volume":"7","author":"Fern\u00e1ndez-Rodr\u00edguez","year":"2014","journal-title":"Fungal Ecol"},{"key":"2025121601050692000_7.11.e018109.36","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1080\/00173134.2011.596218","article-title":"Two sampling methods for the Petri dish detection of airborne fungi","volume":"50","author":"Fern\u00e1ndez Rodr\u00edguez","year":"2011","journal-title":"Grana"},{"key":"2025121601050692000_7.11.e018109.37","doi-asserted-by":"publisher","DOI":"10.1007\/s11046-013-9712-6"},{"key":"2025121601050692000_7.11.e018109.38","doi-asserted-by":"publisher","DOI":"10.1053\/jhin.2000.0773"},{"key":"2025121601050692000_7.11.e018109.39","doi-asserted-by":"publisher","DOI":"10.1053\/jhin.1998.0590"},{"key":"2025121601050692000_7.11.e018109.40","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2004.10.009"},{"key":"2025121601050692000_7.11.e018109.41","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01159-11"},{"key":"2025121601050692000_7.11.e018109.42","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cir771"},{"key":"2025121601050692000_7.11.e018109.43","doi-asserted-by":"crossref","first-page":"157","DOI":"10.12688\/f1000research.10216.1","article-title":"Challenges in microbiological diagnosis of invasive Aspergillus infections","volume":"6","author":"Alanio","year":"2017","journal-title":"F1000Res"},{"key":"2025121601050692000_7.11.e018109.44","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2007.01.009"},{"key":"2025121601050692000_7.11.e018109.45","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2008.02656.x"},{"key":"2025121601050692000_7.11.e018109.46","doi-asserted-by":"publisher","DOI":"10.3201\/eid1710.110226"},{"key":"2025121601050692000_7.11.e018109.47","doi-asserted-by":"publisher","DOI":"10.1016\/j.medmal.2013.02.010"},{"key":"2025121601050692000_7.11.e018109.48","doi-asserted-by":"crossref","first-page":"pii: 20326","DOI":"10.2807\/ese.17.48.20326-en","article-title":"Azole-resistant Aspergillus fumigatus due to TR46\/Y121F\/T289A mutation emerging in Belgium, July 2012","volume":"17","author":"Vermeulen","year":"2012","journal-title":"Euro Surveill"},{"key":"2025121601050692000_7.11.e018109.49","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00231-09"},{"key":"2025121601050692000_7.11.e018109.50","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiac.2015.11.009"},{"key":"2025121601050692000_7.11.e018109.51","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cit320"},{"key":"2025121601050692000_7.11.e018109.52","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.1.494-503.2005"},{"key":"2025121601050692000_7.11.e018109.53","doi-asserted-by":"publisher","DOI":"10.1055\/s-0035-1562893"},{"key":"2025121601050692000_7.11.e018109.54","doi-asserted-by":"publisher","DOI":"10.1136\/thoraxjnl-2014-206291"},{"key":"2025121601050692000_7.11.e018109.55","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200401-093OC"},{"key":"2025121601050692000_7.11.e018109.56","unstructured":"American Academy of Pediatrics. Committee on Infectious Diseases. Red Book. 28th edition. Elk Grove Village: IL, 2009."},{"key":"2025121601050692000_7.11.e018109.57","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-294X.2011.05364.x"},{"key":"2025121601050692000_7.11.e018109.58","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.envint.2016.11.029","article-title":"From the exposome to mechanistic understanding of chemical-induced adverse effects","volume":"99","author":"Escher","year":"2017","journal-title":"Environ Int"}],"container-title":["BMJ Open"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/bmjopen-2017-018109","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T09:05:26Z","timestamp":1765875926000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmjopen.bmj.com\/lookup\/doi\/10.1136\/bmjopen-2017-018109"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,11]]},"references-count":58,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2017,11,25]]},"published-print":{"date-parts":[[2017,11]]}},"alternative-id":["10.1136\/bmjopen-2017-018109"],"URL":"https:\/\/doi.org\/10.1136\/bmjopen-2017-018109","relation":{},"ISSN":["2044-6055","2044-6055"],"issn-type":[{"value":"2044-6055","type":"print"},{"value":"2044-6055","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,11]]}}}