{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,12]],"date-time":"2026-11-12T14:28:31Z","timestamp":1794493711873,"version":"4.9.1"},"reference-count":44,"publisher":"BMJ","issue":"5","license":[{"start":{"date-parts":[[2022,5,30]],"date-time":"2022-05-30T00:00:00Z","timestamp":1653868800000},"content-version":"unspecified","delay-in-days":29,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009087","name":"Universidad Industrial de Santander","doi-asserted-by":"publisher","award":["\"Convocatoria Santander Cientifico\" Project code 2"],"award-info":[{"award-number":["\"Convocatoria Santander Cientifico\" Project code 2"]}],"id":[{"id":"10.13039\/501100009087","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["BMJ Open"],"accepted":{"date-parts":[[2022,5,13]]},"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>Preterm birth (PTB) is a public health issue. Interventions to prolong the length of gestation have not achieved the expected results, as the selection of population at risk of PTB is still a challenge. Cervical length (CL) is the most accepted biomarker, however in the best scenario the CL identifies half of the patients. It is unlikely that a single measure identifies all pregnant women who will deliver before 37 weeks of gestation, considering the multiple pathways theory. We planned this cohort to study the link between the vaginal microbiome, the proteome, metabolome candidates, characteristics of the cervix and the PTB.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Participants<\/jats:title>\n                    <jats:p>Pregnant women in the first trimester of a singleton pregnancy are invited to participate in the study. We are collecting biological samples, including vaginal fluid and blood from every patient, also performing ultrasound measurement that includes Consistency Cervical Index (CCI) and CL. The main outcome is the delivery of a neonate before 37 weeks of gestation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Findings to date<\/jats:title>\n                    <jats:p>We have recruited 244 pregnant women. They all have measurements of the CL and CCI. A vaginal sample for microbiome analysis has been collected in the 244 patients. Most of them agreed to blood collection, 216 (89%). By August 2021, 100 participants had already delivered. Eleven participants (11 %) had a spontaneous PTB.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Future plans<\/jats:title>\n                    <jats:p>A reference value chart for the first trimester CCI will be created. We will gather information regarding the feasibility, reproducibility and limitations of CCI. Proteomic and metabolomic analyses will be done to identify the best candidates, and we will validate their use as predictors. Finally, we plan to integrate clinical data, ultrasound measurements and biological profiles into an algorithm to obtain a multidimensional biomarker to identify the individual risk for PTB.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/bmjopen-2021-060556","type":"journal-article","created":{"date-parts":[[2022,5,30]],"date-time":"2022-05-30T11:51:41Z","timestamp":1653911501000},"page":"e060556","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":9,"title":["Cohort profile: Colombian Cohort for the Early Prediction of Preterm Birth (COLPRET): early prediction of preterm birth based on personal medical history, clinical characteristics, vaginal microbiome, biophysical characteristics of the cervix and maternal serum biochemical markers"],"prefix":"10.1136","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3188-530X","authenticated-orcid":false,"given":"Carlos Hernan","family":"Becerra-Mojica","sequence":"first","affiliation":[{"name":"Obstetrics and Gynecology Department, Health Faculty, Universidad Industrial de Santander, Bucaramanga, Colombia"},{"name":"Maternal-fetal Medicine Unit, Hospital Universitario de Santander, Bucaramanga, Colombia"},{"name":"Centro de Atencion Materno-fetal INUTERO, Bucaramanga, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2287-000X","authenticated-orcid":false,"given":"Miguel Antonio","family":"Parra-Saavedra","sequence":"additional","affiliation":[{"name":"Obstetrics and Gynecology Department, Universidad Libre, Barranquilla, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4498-6639","authenticated-orcid":false,"given":"Luis Alfonso","family":"Diaz-Martinez","sequence":"additional","affiliation":[{"name":"School of Medicine . Health Faculty, Universidad Industrial de Santander, Bucaramanga, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4711-3857","authenticated-orcid":false,"given":"Raigam Jafet","family":"Martinez-Portilla","sequence":"additional","affiliation":[{"name":"Clinical Research Division, National Institute of Perinatology, Mexico City, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5323-0422","authenticated-orcid":false,"given":"Bladimiro","family":"Rincon Orozco","sequence":"additional","affiliation":[{"name":"Basics Sciences Department, Health Faculty, Universidad Industrial de Santander, Bucaramanga, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[2022,5,30]]},"reference":[{"key":"2025121601423173000_12.5.e060556.1","unstructured":"March of Dimes, PMNCH, Save the children, Who Howson CP , Kinney MV , Lawn JE , eds. Born too soon: the global action report on preterm birth. Geneva: World health organization, 2012. https:\/\/www.who.int\/en\/news-room\/fact sheets\/detail\/pretermbirth"},{"key":"2025121601423173000_12.5.e060556.2","doi-asserted-by":"publisher","DOI":"10.1016\/s0140-6736(16)31593-8"},{"key":"2025121601423173000_12.5.e060556.3","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(08)60136-1"},{"key":"2025121601423173000_12.5.e060556.4","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(18)32281-5"},{"key":"2025121601423173000_12.5.e060556.5","doi-asserted-by":"publisher","DOI":"10.1016\/s2214-109x(18)30451-0"},{"key":"2025121601423173000_12.5.e060556.6","unstructured":"Sivigila . Instituto Nacional de Salud. Colombia, 2019."},{"key":"2025121601423173000_12.5.e060556.7","unstructured":"WHO . Who recommendations on interventions to improve preterm birth outcomes, 2015: 98. ISBN: 9789241508988. https:\/\/www.who.int\/reproductivehealth\/publications\/maternal_perinatal_health\/preterm-birth-guideline\/en\/"},{"key":"2025121601423173000_12.5.e060556.8","article-title":"Magnesium maintenance therapy for preventing preterm birth after threatened preterm labour","volume":"5","author":"Han","year":"2013","journal-title":"Cochrane Database Syst Rev"},{"key":"2025121601423173000_12.5.e060556.9","first-page":"CD004071","article-title":"Maintenance therapy with calcium channel blockers for preventing preterm birth after threatened preterm labour","volume":"10","author":"Naik Gaunekar","year":"2013","journal-title":"Cochrane Database Syst Rev"},{"key":"2025121601423173000_12.5.e060556.10","article-title":"Maintenance therapy with oxytocin antagonists for inhibiting preterm birth after threatened preterm labour","volume":"10","author":"Papatsonis","year":"2013","journal-title":"Cochrane Database Syst Rev"},{"key":"2025121601423173000_12.5.e060556.11","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0109069","article-title":"Brazilian multicentre study on preterm birth (EMIP): prevalence and factors associated with spontaneous preterm birth","volume":"9","author":"Passini","year":"2014","journal-title":"PLoS One"},{"key":"2025121601423173000_12.5.e060556.12","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen-2016-015402"},{"key":"2025121601423173000_12.5.e060556.13","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa035140"},{"key":"2025121601423173000_12.5.e060556.14","doi-asserted-by":"publisher","DOI":"10.1067\/mob.2003.41"},{"key":"2025121601423173000_12.5.e060556.15","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199602293340904"},{"key":"2025121601423173000_12.5.e060556.16","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-0705.1998.12050312.x"},{"key":"2025121601423173000_12.5.e060556.17","doi-asserted-by":"publisher","DOI":"10.1002\/uog.5333"},{"key":"2025121601423173000_12.5.e060556.18","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2017.11.576"},{"key":"2025121601423173000_12.5.e060556.19","article-title":"Midtrimester cervical length in low-risk nulliparous women for the prediction of spontaneous preterm birth: should we consider a new definition of short cervix?","author":"Guerby","year":"2021","journal-title":"Am J Perinatol"},{"key":"2025121601423173000_12.5.e060556.20","doi-asserted-by":"crossref","first-page":"288.e1","DOI":"10.1016\/j.ajog.2020.09.002","article-title":"Personalized assessment of cervical length improves prediction of spontaneous preterm birth: a standard and a percentile calculator","volume":"224","author":"Gudicha","year":"2021","journal-title":"Am J Obstet Gynecol"},{"key":"2025121601423173000_12.5.e060556.21","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1002\/uog.9010","article-title":"Prediction of preterm birth using the cervical consistency index","volume":"38","author":"Parra-Saavedra","year":"2011","journal-title":"Ultrasound Obstet Gynecol"},{"key":"2025121601423173000_12.5.e060556.22","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.bpobgyn.2018.03.006","article-title":"The microbiome in preterm birth","volume":"52","author":"Chu","year":"2018","journal-title":"Best Pract Res Clin Obstet Gynaecol"},{"key":"2025121601423173000_12.5.e060556.23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2018\/7218187","article-title":"The microbiome and preterm birth: a change in paradigm with profound implications for pathophysiologic concepts and novel therapeutic strategies","volume":"2018","author":"Staude","year":"2018","journal-title":"Biomed Res Int"},{"key":"2025121601423173000_12.5.e060556.24","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1705899114"},{"key":"2025121601423173000_12.5.e060556.25","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.jprot.2018.02.002","article-title":"Proteomic analysis of first trimester maternal serum to identify candidate biomarkers potentially predictive of spontaneous preterm birth","volume":"178","author":"D'Silva","year":"2018","journal-title":"J Proteomics"},{"key":"2025121601423173000_12.5.e060556.26","doi-asserted-by":"publisher","DOI":"10.1210\/en.2018-00073"},{"key":"2025121601423173000_12.5.e060556.27","doi-asserted-by":"crossref","first-page":"493.e1","DOI":"10.1016\/j.ajog.2019.11.1252","article-title":"Cervicovaginal fluid proteomic analysis to identify potential biomarkers for preterm birth","volume":"222","author":"Parry","year":"2020","journal-title":"Am J Obstet Gynecol"},{"key":"2025121601423173000_12.5.e060556.28","doi-asserted-by":"publisher","DOI":"10.1021\/pr0605421"},{"key":"2025121601423173000_12.5.e060556.29","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.05.002"},{"key":"2025121601423173000_12.5.e060556.30","doi-asserted-by":"publisher","DOI":"10.1007\/s11306-016-1094-6"},{"key":"2025121601423173000_12.5.e060556.31","doi-asserted-by":"crossref","first-page":"1213","DOI":"10.1093\/bioinformatics\/btz736","article-title":"Identification of metabolites from tandem mass spectra with a machine learning approach utilizing structural features","volume":"36","author":"Li","year":"2020","journal-title":"Bioinformatics"},{"key":"2025121601423173000_12.5.e060556.32","doi-asserted-by":"crossref","DOI":"10.3389\/fbioe.2018.00031","article-title":"A machine learning application based in random forest for integrating mass spectrometry-based metabolomic data: a simple screening method for patients with Zika virus","volume":"6","author":"Melo","year":"2018","journal-title":"Front Bioeng Biotechnol"},{"key":"2025121601423173000_12.5.e060556.33","doi-asserted-by":"publisher","DOI":"10.1126\/science.1251816"},{"key":"2025121601423173000_12.5.e060556.34","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1038\/s41390-020-0981-8","article-title":"Towards personalized medicine in maternal and child health: integrating biologic and social determinants","volume":"89","author":"Stevenson","year":"2021","journal-title":"Pediatr Res"},{"key":"2025121601423173000_12.5.e060556.35","doi-asserted-by":"publisher","DOI":"10.1002\/uog.12342"},{"key":"2025121601423173000_12.5.e060556.36","unstructured":"The fetal medicine foundation . The 11-13 weeks scan | Education | Welcome to the Fetal Medicine Foundation [Internet]; 2019. https:\/\/fetalmedicine.org\/education\/the-11-13-weeks-scan [Accessed cited 2019 Apr 23]."},{"key":"2025121601423173000_12.5.e060556.37","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-0705.1998.12050304.x"},{"key":"2025121601423173000_12.5.e060556.38","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.2147\/IJWH.S265130","article-title":"A novel strategy for cervical cancer prevention using Cervical-Vaginal self-collected samples shows high acceptability in women living in low-income conditions from Bucaramanga, Colombia","volume":"12","author":"Torrado-Garc\u00eda","year":"2020","journal-title":"Int J Womens Health"},{"key":"2025121601423173000_12.5.e060556.39","doi-asserted-by":"publisher","DOI":"10.1002\/uog.5333"},{"key":"2025121601423173000_12.5.e060556.40","doi-asserted-by":"crossref","DOI":"10.1371\/annotation\/50c43133-0df5-4b8b-8975-8cc37d4f2f26","article-title":"A method for studying protistan diversity using massively parallel sequencing of v9 hypervariable regions of small-subunit ribosomal RNA genes","volume":"4","author":"Amaral-Zettler","year":"2009","journal-title":"PLoS One"},{"key":"2025121601423173000_12.5.e060556.41","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1111\/j.1365-294X.2009.04480.x","article-title":"Multiple marker parallel tag environmental DNA sequencing reveals a highly complex eukaryotic community in marine anoxic water","volume":"19 Suppl 1","author":"Stoeck","year":"2010","journal-title":"Mol Ecol"},{"key":"2025121601423173000_12.5.e060556.42","doi-asserted-by":"crossref","unstructured":"Keegan KP , Glass EM , Meyer F . MG-RAST, a Metagenomics Service for Analysis of Microbial Community Structure and Function. In: Methods in molecular biology. Clifton, NJ, 2016: 207\u201333. http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/26791506","DOI":"10.1007\/978-1-4939-3369-3_13"},{"key":"2025121601423173000_12.5.e060556.43","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pcbi.1004957","article-title":"MEGAN community edition - interactive exploration and analysis of large-scale microbiome sequencing data","volume":"12","author":"Huson","year":"2016","journal-title":"PLoS Comput Biol"},{"key":"2025121601423173000_12.5.e060556.44","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu494"}],"container-title":["BMJ Open"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/bmjopen-2021-060556","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T09:42:44Z","timestamp":1765878164000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmjopen.bmj.com\/lookup\/doi\/10.1136\/bmjopen-2021-060556"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5]]},"references-count":44,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2022,5,30]]},"published-print":{"date-parts":[[2022,5]]}},"alternative-id":["10.1136\/bmjopen-2021-060556"],"URL":"https:\/\/doi.org\/10.1136\/bmjopen-2021-060556","relation":{},"ISSN":["2044-6055","2044-6055"],"issn-type":[{"value":"2044-6055","type":"print"},{"value":"2044-6055","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5]]}}}