{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T01:21:51Z","timestamp":1792718511024,"version":"4.5.0"},"reference-count":39,"publisher":"BMJ","issue":"12","license":[{"start":{"date-parts":[[2022,12,13]],"date-time":"2022-12-13T00:00:00Z","timestamp":1670889600000},"content-version":"unspecified","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["BMJ Open"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>We aim to evaluate salivary matrix metalloproteinases (MMP-8) levels in oral submucous fibrosis (OSF) and oral squamous cell carcinoma (OSCC) for the purpose of diagnosis at the early stage via non-invasive method.<\/jats:p><\/jats:sec><jats:sec><jats:title>Setting<\/jats:title><jats:p>The study was multicentre, carried out at a tertiary care hospital in Karachi, Pakistan.<\/jats:p><\/jats:sec><jats:sec><jats:title>Participants<\/jats:title><jats:p>A total 60 participants of any age, sex and ethnicity were randomly selected for the purpose of this study. Patients demonstrating clinical evidence of OSF and biopsy-proven cases of OSCC were included. Patients with indeterminate histopathological report, immunodeficiency, autoimmune disorder, chronic medical and periodontal disease (periodontal depth greater than 5\u2009mm) and individuals with interincisal mouth opening greater than 35\u2009mm were excluded from the study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Interventions<\/jats:title><jats:p>Salivary MMP-8 levels were observed in OSF, healthy and OSCC groups by using ELISA. One way analysis of variance was applied to establish whether MMP-8 levels of disease-free individuals and patients suffering from OSF and OSCC differed from each other.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Statistically significant difference in salivary MMP-8 expression in diseased and control group was observed. MMP-8 levels in OSCC (0.64\u2009ng\/mL) and OSF (0.66\u2009ng\/mL) were underexpressed as compared with healthy participants (7.9\u2009ng\/mL).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>MMP-8 levels were underexpressed in OSCC and OSF patients as compared with controls, which imply that MMP-8 level has an inverse relation with OSCC and OSF.<\/jats:p><\/jats:sec>","DOI":"10.1136\/bmjopen-2021-060738","type":"journal-article","created":{"date-parts":[[2022,12,13]],"date-time":"2022-12-13T16:03:55Z","timestamp":1670947435000},"page":"e060738","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":8,"title":["Relation of salivary MMP-8 with oral submucous fibrosis and oral squamous cell carcinoma: a cross sectional analytical study"],"prefix":"10.1136","volume":"12","author":[{"given":"Anum","family":"Kazmi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zia","family":"Abbas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8148-6596","authenticated-orcid":false,"given":"Zohra","family":"Saleem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Safdar","family":"Haider","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8773-8029","authenticated-orcid":false,"given":"Waqas Ahmed","family":"Farooqui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3571-982X","authenticated-orcid":false,"given":"Shaheen","family":"Ahmed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[2022,12,13]]},"reference":[{"key":"2022123021453534000_12.12.e060738.1","doi-asserted-by":"crossref","DOI":"10.3390\/ijms20184506","article-title":"The role of MMP8 in cancer: a systematic review","volume":"20","author":"Juurikka","year":"2019","journal-title":"Int J Mol Sci"},{"key":"2022123021453534000_12.12.e060738.2","doi-asserted-by":"publisher","DOI":"10.1186\/s12903-020-01373-3"},{"key":"2022123021453534000_12.12.e060738.3","doi-asserted-by":"crossref","unstructured":"Farah CS , Shearston K , Nguyen AP . 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