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Secondarily, to investigate vitamin B\n                      <jats:sub>12<\/jats:sub>\n                      (B\n                      <jats:sub>12<\/jats:sub>\n                      ) deficiency as a cause for DPN in adolescents with T1D.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Research design and methods<\/jats:title>\n                    <jats:p>\n                      Cross-sectional study. Sixty Danish adolescents with T1D (age 15\u201318 years, diabetes duration &gt;5 years) and 23 age-matched control subjects were included. Based on nerve conduction studies (NCS), intraepidermal nerve fibre density (IENFD) and neurological examination, patients were divided into three groups: (1) T1D without DPN, (2) T1D with subclinical DPN and (3) T1D with confirmed DPN. Blood levels of NfL, B\n                      <jats:sub>12<\/jats:sub>\n                      , B\n                      <jats:sub>12<\/jats:sub>\n                      -binding protein holotranscobalamin (HoloTC) and methylmalonic acid (MMA) were determined.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Twenty-four of the adolescents were without DPN, twenty-one had subclinical DPN and eight had confirmed DPN. NCS was not conducted in three participants and four patients did not have blood samples taken. There were no significant differences in NfL levels or any of the B\n                      <jats:sub>12<\/jats:sub>\n                      parameters between any of the groups.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      NfL used in a cross-sectional manner was not found useful to distinguish between the adolescents with DPN and those without. Vitamin B\n                      <jats:sub>12<\/jats:sub>\n                      deficiency did not contribute to neuropathy in Danish adolescents with T1D.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/bmjopen-2024-085749","type":"journal-article","created":{"date-parts":[[2025,3,3]],"date-time":"2025-03-03T16:55:15Z","timestamp":1741020915000},"page":"e085749","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":3,"title":["Biochemical use of neurofilament light polypeptide and vitamin B\n                    <sub>12<\/sub>\n                    in relation to diabetic polyneuropathy in Danish adolescents with type 1 diabetes: a cross-sectional study"],"prefix":"10.1136","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3557-6566","authenticated-orcid":false,"given":"Mathilde","family":"Thrys\u00f8e","sequence":"first","affiliation":[{"name":"Department of Neurology, Aarhus University Hospital, Aarhus, Denmark"},{"name":"Medical Diagnostic Center, Viborg Regional Hospital, Viborg, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tina","family":"Parkner","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry, Aarhus University Hospital, Aarhus, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hatice","family":"Tankisi","sequence":"additional","affiliation":[{"name":"Department of Neurophysiology, Aarhus University Hospital, Aarhus, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jens Randel","family":"Nyengaard","sequence":"additional","affiliation":[{"name":"Core Centre for Molecular Morphology, Section for Stereology and Microscopy, Department of Clinical Medicine, Aarhus University, Aarhus, Denmark"},{"name":"Department of Pathology, Aarhus University Hospital, Aarhus, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esben Thyssen","family":"Vestergaard","sequence":"additional","affiliation":[{"name":"Department of Pediatric and Adolescents Medicine, Aarhus University Hospital, Aarhus, Denmark"},{"name":"Steno Diabetes Center Aarhus, Aarhus University Hospital, Aarhus, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kurt","family":"Kristensen","sequence":"additional","affiliation":[{"name":"Steno Diabetes Center Aarhus, Aarhus University Hospital, Aarhus, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Astrid Juhl","family":"Terkelsen","sequence":"additional","affiliation":[{"name":"Department of Neurology, Aarhus University Hospital, Aarhus, Denmark"},{"name":"Danish Pain Research Center, Aarhus University, Aarhus, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1356-2833","authenticated-orcid":false,"given":"Vinni Faber","family":"Rasmussen","sequence":"additional","affiliation":[{"name":"Department of Pediatrics and Adolescents, Randers Regional Hospital, Randers, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[2025,3,3]]},"reference":[{"key":"2025121509363646000_15.3.e085749.1","unstructured":"Graham D Ogle FW, G. 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