{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T15:28:21Z","timestamp":1787671701168,"version":"build-2736575974"},"reference-count":48,"publisher":"BMJ","issue":"8","license":[{"start":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:00:00Z","timestamp":1787616000000},"content-version":"unspecified","delay-in-days":24,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82374221"],"award-info":[{"award-number":["82374221"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National High Level Hospital Clinical Research Funding","award":["2023-NHLHCRF-YYPPLC-ZR-10"],"award-info":[{"award-number":["2023-NHLHCRF-YYPPLC-ZR-10"]}]},{"name":"National High Level Hospital Clinical Research Funding","award":["2025-NHLHCRF-JBGS-A-WZ-12"],"award-info":[{"award-number":["2025-NHLHCRF-JBGS-A-WZ-12"]}]}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["BMJ Open"],"accepted":{"date-parts":[[2026,8,2]]},"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>The passive mast cell activation test (pMAT) is a novel in vitro assay designed to complement existing tools like the skin test (ST) and basophil activation test (BAT) for allergen identification. This study aimed to validate the diagnostic accuracy of pMAT specifically for neuromuscular blocking agent (NMBA)-induced perioperative anaphylaxis (POA).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Design<\/jats:title>\n                    <jats:p>A diagnostic accuracy study.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Setting<\/jats:title>\n                    <jats:p>A tertiary hospital in Beijing, China.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Participants<\/jats:title>\n                    <jats:p>44 patients with suspected POA were enrolled. Participants were stratified into groups based on ST and\/or BAT. The NMBA-allergic group comprised 15 patients with confirmed NMBA allergy while the non-NMBA-allergic group consisted of 29 individuals without NMBA allergy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Interventions<\/jats:title>\n                    <jats:p>All participants underwent pMAT testing for relevant NMBA.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Primary and secondary outcome measures<\/jats:title>\n                    <jats:p>The diagnostic performance of pMAT for NMBA was assessed by measuring sensitivity, specificity, likelihood ratios and the Youden index. An exploratory analysis evaluated its performance in diagnosing antibiotic-induced allergies within the same population.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>For NMBA allergy, pMAT demonstrated high diagnostic accuracy (90.9%; 95%\u2009CI 77.4% to 97.1%) with excellent sensitivity (93.3%; 95%\u2009CI 66.0% to 99.7%)\u2009and specificity (89.7%; 95%\u2009CI 71.5% to 97.3%). This was reflected in a high positive likelihood ratio (9.05; 95%\u2009CI 3.06 to 26.57), a low negative likelihood ratio (0.07; 95%\u2009CI 0.01 to 0.50), a Youden index of 0.83 and a Cohen\u2019s kappa of 0.804 (indicating substantial agreement with the reference standard). In contrast, pMAT showed reduced performance for antibiotic allergy, with an accuracy of 79.5% (95% CI 64.3% to 89.7%), sensitivity of 66.7% (95% CI 30.9% to 91.0%), specificity of 82.9% (95% CI 65.7% to 92.8%) and a kappa of 0.378 (fair agreement).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>pMAT demonstrated high diagnostic accuracy for NMBA allergy, filling critical gaps in current POA diagnostic workflow. Its robust performance supports its clinical utility as a complementary tool alongside ST and BAT.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Trial registration number<\/jats:title>\n                    <jats:p>ChiCTR2400084268.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/bmjopen-2026-118254","type":"journal-article","created":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T14:46:23Z","timestamp":1787669183000},"page":"e118254","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":0,"title":["Diagnostic accuracy of the passive mast cell activation test in neuromuscular blocking agent-induced perioperative anaphylaxis in a tertiary hospital in China: a diagnostic accuracy 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