{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T19:36:34Z","timestamp":1788982594067,"version":"build-2803163510"},"reference-count":65,"publisher":"BMJ","issue":"1","license":[{"start":{"date-parts":[[2022,9,14]],"date-time":"2022-09-14T00:00:00Z","timestamp":1663113600000},"content-version":"unspecified","delay-in-days":13,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["BMJ Open Ophth"],"accepted":{"date-parts":[[2022,8,30]]},"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:sec>\n                  <jats:title>Objective<\/jats:title>\n                  <jats:p>One of the most important risk factors for developing a glaucomatous optic neuropathy is elevated intraocular pressure. Moreover, mechanisms such as altered perfusion have been postulated to injure the optical path. In a mouse model, we compare first negative effects of cerebral perfusion\/reperfusion on the optic nerve structure versus alterations by elevated intraocular pressure. Second, we compare the alterations by isolated hypoperfusion-reperfusion and isolated intraocular pressure to the combination of both.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods and analysis<\/jats:title>\n                  <jats:p>Mice were divided in four groups: (1) controls; (2) perfusion altered mice that underwent transient bi-common carotid artery occlusion (BCCAO) for 40\u2009min; (3) glaucoma group (DBA\/2J mice); (4) combined glaucoma and altered perfusion (DBA\/2J mice with transient BCCAO). Optic nerve sections were stereologically examined 10\u201312 weeks after intervention.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>All experimental groups showed a decreased total axon number per optic nerve compared with controls. In DBA\/2J and combined DBA\/2J &amp; BCCAO mice the significant decrease was roughly 50%, while BCCAO leaded to a 23% reduction of axon number, however reaching significance only in the direct t-test. The difference in axon number between BCCAO and both DBA\/2J mice was almost 30%, lacking statistical significance due to a remarkably high variation in both DBA\/2J groups.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>Elevated intraocular pressure in the DBA\/2J mouse model of glaucoma leads to a much more pronounced optic nerve atrophy compared with transient forebrain hypoperfusion and reperfusion by BCCAO. A supposed worsening effect of an altered perfusion added to the pressure-related damage could not be detected.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1136\/bmjophth-2022-001078","type":"journal-article","created":{"date-parts":[[2022,9,14]],"date-time":"2022-09-14T12:00:26Z","timestamp":1663156826000},"page":"e001078","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":3,"title":["Impact of cerebral hypoperfusion\u2013reperfusion on optic nerve integrity and visual function in the DBA\/2J mouse model of glaucoma"],"prefix":"10.1136","volume":"7","author":[{"given":"Stephan Alexander","family":"Fraenkl","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Inselspital, University of Bern, Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quentin","family":"Simon","sequence":"additional","affiliation":[{"name":"Institute of Anatomy, University of Bern, Bern, 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