{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T17:35:17Z","timestamp":1782236117749,"version":"3.54.5"},"reference-count":24,"publisher":"BMJ","issue":"1","license":[{"start":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T00:00:00Z","timestamp":1765756800000},"content-version":"unspecified","delay-in-days":14,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Berta-Ottenstein-Programm for Advanced Clinical Scientists, from the Faculty of Medicine, University of Freiburg"}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["BMJ Open Resp Res"],"accepted":{"date-parts":[[2025,11,11]]},"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Non-necrotising granulomas are the histological hallmark of sarcoidosis and chronic beryllium disease (CBD). Endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA), transbronchial lung forceps biopsy (TBLB) and endobronchial biopsy (EBB) are often used as standard methods for obtaining suitable tissue. Transbronchial lung cryobiopsy (TBLC) is a novel biopsy technique well studied in different fibrosing lung diseases; however, data on its use in sarcoidosis are scarce. This study aimed to assess whether TBLC provides additional diagnostic value compared with standard diagnostic methods in detecting non-necrotising granulomas.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We retrospectively analysed 321 patients diagnosed with sarcoidosis or CBD between 2010 and 2020 in a single tertiary care centre. Patients who underwent intrathoracic biopsy were analysed to assess the diagnostic yield, factors influencing diagnostic success, as well as complications related to each technique.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Intrathoracic biopsy procedures were performed on 264 patients (EBUS-TBNA n=215, EBB n=61, TBLB n=120\u2009and TBLC n=66). The diagnostic yields for single methods ranged from 56.3% (EBB) to 63.6% (TBLC) (p=0.7643). Combination of EBUS-TBNA with TBLC increased the diagnostic yield to 91.7%. Notably, TBLC provided a superior diagnostic yield compared with TBB in cases without radiologically detected parenchymal involvement. Complication rates were numerically higher following TBLC compared with TBLB (16.7% vs 9.2%, p=0.1561).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The addition of TBLC significantly enhances the diagnostic yield in the workup of sarcoidosis and CBD, particularly in cases without radiologically detected parenchymal involvement. This underscores the added value of TBLC in improving diagnostic accuracy in challenging clinical scenarios.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/bmjresp-2025-003206","type":"journal-article","created":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T10:30:20Z","timestamp":1765967420000},"page":"e003206","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":1,"title":["Additional diagnostic potential of transbronchial lung cryobiopsy in bronchoscopic assessment of sarcoidosis and chronic beryllium disease: a retrospective analysis of 321 patients"],"prefix":"10.1136","volume":"12","author":[{"given":"Charlott","family":"Terschluse","sequence":"first","affiliation":[{"name":"Department for Pneumology, University of Freiburg, Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Feineis","sequence":"additional","affiliation":[{"name":"Department for Pneumology, University of Freiburg, Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louis","family":"Jouanjan","sequence":"additional","affiliation":[{"name":"Department for Pneumology, University of Freiburg, Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Soriano","sequence":"additional","affiliation":[{"name":"Department for Pneumology, University of Freiburg, Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfram","family":"Meschede","sequence":"additional","affiliation":[{"name":"Department for Pneumology, University of Freiburg, Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"F\u00e4hndrich","sequence":"additional","affiliation":[{"name":"Department for Pneumology, University of Freiburg, Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joachim","family":"M\u00fcller-Quernheim","sequence":"additional","affiliation":[{"name":"Department for Pneumology, University of Freiburg, Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gernot","family":"Zissel","sequence":"additional","affiliation":[{"name":"Department for Pneumology, University of Freiburg, Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daiana","family":"Stolz","sequence":"additional","affiliation":[{"name":"Department for Pneumology, University of Freiburg, Freiburg, Germany"},{"name":"University Hospital Basel, Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prerana","family":"Agarwal","sequence":"additional","affiliation":[{"name":"Department for Radiology, University of Freiburg, Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3520-1868","authenticated-orcid":false,"given":"Bj\u00f6rn Christian","family":"Frye","sequence":"additional","affiliation":[{"name":"Department for Pneumology, University of Freiburg, Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[2025,12,17]]},"reference":[{"key":"2026010701524189000_12.1.e003206.1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.202002-0251ST"},{"key":"2026010701524189000_12.1.e003206.2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201409-1722ST"},{"key":"2026010701524189000_12.1.e003206.3","doi-asserted-by":"publisher","DOI":"10.1016\/j.rmed.2012.02.014"},{"key":"2026010701524189000_12.1.e003206.4","doi-asserted-by":"publisher","DOI":"10.1097\/LBR.0000000000000081"},{"key":"2026010701524189000_12.1.e003206.5","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1111\/crj.12304","article-title":"The role of EBUS-TBNA and standard bronchoscopic modalities in the diagnosis of sarcoidosis","volume":"11","author":"Dziedzic","year":"2017","journal-title":"Clin Respir 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with Sarcoidosis in a Prospective, Randomized, Multicentre Cross-Over Trial","volume":"10","author":"H\u00e4ntschel","year":"2021","journal-title":"J Clin Med"},{"key":"2026010701524189000_12.1.e003206.16","doi-asserted-by":"publisher","DOI":"10.1159\/000203987"},{"key":"2026010701524189000_12.1.e003206.17","doi-asserted-by":"crossref","DOI":"10.1186\/s12931-019-1091-1","article-title":"Bleeding risk of transbronchial cryobiopsy compared to transbronchial forceps biopsy in interstitial lung disease - a prospective, randomized, multicentre cross-over trial","volume":"20","author":"Hetzel","year":"2019","journal-title":"Respir Res"},{"key":"2026010701524189000_12.1.e003206.18","doi-asserted-by":"crossref","DOI":"10.1183\/13993003.01520-2019","article-title":"Transbronchial cryobiopsy increases diagnostic confidence in interstitial lung disease: a prospective multicentre trial","volume":"56","author":"Hetzel","year":"2020","journal-title":"Eur Respir 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