{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T10:12:29Z","timestamp":1765620749325,"version":"3.48.0"},"reference-count":18,"publisher":"BMJ","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"published-print":{"date-parts":[[1971,3]]},"abstract":"<jats:p>\n                    Total protein and deoxyribonucleic acid (\n                    <jats:sc>dna<\/jats:sc>\n                    ) were measured in small-intestinal washings from rats with normal and high rates of\n                    <jats:sc>dna<\/jats:sc>\n                    (or cell) loss. There was a significant relationship between the loss of these two substances. Preparations of isolated viable epithelial cells contained much less protein in relation to\n                    <jats:sc>dna<\/jats:sc>\n                    than did the intestinal washings. It was calculated that only 8 to 15% of the protein lost by washing the rat small intestine arose from the intracellular protein of exfoliated epithelial cells. The rest was derived from extracellular sources.\n                  <\/jats:p>\n                  <jats:p>\n                    Protein and\n                    <jats:sc>dna<\/jats:sc>\n                    loss from small-bowel mucosa was measured in six patients. The ratio of protein to\n                    <jats:sc>dna<\/jats:sc>\n                    was similar to that found in the rats. The mean protein loss from 5 cm of human upper small intestine was 956 \u03bcg per min or 1\u00b74 g per 24 hours. By calculation, total protein loss from the whole small intestine of man was about 84 g per day, about 10 g coming from within exfoliated cells and the rest arising from extracellular sources. The normal intestine must reabsorb most of this material.\n                  <\/jats:p>","DOI":"10.1136\/gut.12.3.179","type":"journal-article","created":{"date-parts":[[2007,9,18]],"date-time":"2007-09-18T17:41:31Z","timestamp":1190137291000},"page":"179-183","source":"Crossref","is-referenced-by-count":25,"title":["Protein loss and cell loss from the small-intestinal mucosa"],"prefix":"10.1136","volume":"12","author":[{"given":"L. R.","family":"Da Costa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. N.","family":"Croft","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Creamer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1971,3,1]]},"reference":[{"key":"ref_1","article-title":"Protein and the Small intestine","author":"Costa, L.R.","year":"1970","unstructured":"Costa, L. R. da (1970). Protein and the Small intestine. MD Thesis, London."},{"key":"ref_2","first-page":"491","article-title":"Isolated gastric parietal cells: oxygen consumption, electrolyte content and intracellular pH","volume":"37","author":"Croft, D.N.; Ingelfinger, F.J.","year":"1969","unstructured":"Croft, D. N., and Ingelfinger, F. J. (1969). Isolated gastric parietal cells: oxygen consumption, electrolyte content and intracellular pH. Clin. Sci., 37, 491-501.","journal-title":"Clin. Sci"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/S0140-6736(68)90354-1","article-title":"Small-bowel cell-loss and weight-loss in the coeliac syndrome","volume":"2","author":"Croft, D.N.; Loehry, C.A.; Creamer, B.","year":"1968","unstructured":"Croft, D. N., Loehry, C. A., and Creamer, B. (1968a). Small-bowel cell-loss and weight-loss in the coeliac syndrome. Lancet, 2, 68-70.","journal-title":"Lancet"},{"key":"ref_4","first-page":"70","article-title":"DNA and cell loss from normal small-intestinal mucosa","volume":"2","author":"Croft, D.N.; Loehry, C.A.; Taylor, J.F.N.; Cole, J.","year":"1968","unstructured":"Croft, D. N., Loehry, C. A., Taylor, J. F. N., and Cole, J. (1968b). DNA and cell loss from normal small-intestinal mucosa. Lancet, 2, 70-73.","journal-title":"Lancet"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1042\/bj0950612","article-title":"The estimation of deoxyribonucleic acid in the presence of sialic acid; application to analysis of human gastric washings","volume":"95","author":"Croft, D.N.; Lubran, M.","year":"1965","unstructured":"Croft, D. N., and Lubran, M. (1965). The estimation of deoxyribonucleic acid in the presence of sialic acid; application to analysis of human gastric washings. Biochem. J., 95, 612-620.","journal-title":"Biochem. J"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1038\/203319a0","article-title":"Removal and dissociation of epithelial cells from the rodent gastrointestinal tract","volume":"203","author":"Harrier, D.S.; Stern, B.K.; Reilly, R.W.","year":"1964","unstructured":"Harrier, D. S., Stern, B. K., and Reilly, R. W. (1964). Removal and dissociation of epithelial cells from the rodent gastrointestinal tract. Nature (Lond.), 203, 319-320.","journal-title":"Nature (Lond.)"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"652","DOI":"10.1056\/NEJM196203292661307","article-title":"Plasma proteins and the gastrointestinal tract","volume":"266","author":"Jefferies, G.H.; Holman, H.R.; Sleisenger, M.H.","year":"1962","unstructured":"Jefferies, G. H., Holman, H. R., and Sleisenger, M. H. (1962). Plasma proteins and the gastrointestinal tract. New Engl. J. Med., 266, 652-660.","journal-title":"New Engl. J. Med"},{"key":"ref_8","first-page":"1301","article-title":"Sites of plasma albumin catabolism in the rat","author":"Katz, J.; Rosenfeld, S.; Sellers, A.L.","year":"1961","unstructured":"Katz, J., Rosenfeld, S., and Sellers, A. L. (1961). Sites of plasma albumin catabolism in the rat. Amer. J. Physiol., 200, 1301-1306.","journal-title":"Amer. J. Physiol"},{"year":"1969","author":"Loehry, C.A.; Croft, D.N.; Singh, A.K.; Creamer, B.","key":"ref_9","unstructured":"Loehry, C. A., Croft, D. N., Singh, A. K., and Creamer, B. (1969)."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1007\/BF02232581","article-title":"The role of the digestive tract in protein metabolism","volume":"9","author":"Nasset, E.S.","year":"1964","unstructured":"Nasset, E. S. (1964). The role of the digestive tract in protein metabolism. Amer. J. dig. Dis., 9, 175-190.","journal-title":"Amer. J. dig. Dis"},{"key":"ref_11","article-title":"Role of the digestive system in protein metabolism","author":"Nasset, E.S.","year":"1965","unstructured":"Nasset, E. S. (1965). Role of the digestive system in protein metabolism."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1093\/jn\/74.4.461","article-title":"Mixture of endogenous and exogenous protein in the alimentary tract","volume":"74","author":"Nasset, E.S.; Ju, J.S.","year":"1961","unstructured":"Nasset, E. S., and Ju, J. S. (1961). Mixture of endogenous and exogenous protein in the alimentary tract. J. Nutr., 74, 461-465.","journal-title":"J. Nutr"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1093\/clinchem\/5.6.569","article-title":"A procedure for the determination of cerebrospinal fluid total protein and gamma globulin in neurologic disorders","volume":"5","author":"Papadopoulos, N.M.; Hess, W.C.; O'Doherty, D.; McLane, J.E.","year":"1959","unstructured":"Papadopoulos, N. M., Hess, W. C., O'Doherty, D., and McLane, J. E. (1959). A procedure for the determination of cerebrospinal fluid total protein and gamma globulin in neurologic disorders. Clin. Chem., 5, 569-574.","journal-title":"Clin. Chem"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/0014-4827(57)90013-7","article-title":"Counting actively metabolising tissue cultured cells","volume":"13","author":"Phillips, H.J.; Terryberry, J.E.","year":"1957","unstructured":"Phillips, H. J., and Terryberry, J. E. (1957). Counting actively metabolising tissue cultured cells. Exp. Cell Res., 13, 341-347.","journal-title":"Exp. Cell Res"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1136\/gut.11.3.217","article-title":"Cell loss from small intestinal mucosa: a morphological study","volume":"11","author":"Pink, I.J.; Croft, D.N.; Creamer, B.","year":"1970","unstructured":"Pink, I. J., Croft, D. N., and Creamer, B. (1970). Cell loss from small intestinal mucosa: a morphological study. Gut, 11, 217-222.","journal-title":"Gut"},{"key":"ref_16","first-page":"\"pp. 309","article-title":"Pathology, absorption, transport and activity of digestive enzymes in rat jejunum parasitized by the nematode Nippostrongylus braziliensis","volume":"21","author":"Spencer, R.P.","year":"1960","unstructured":"Spencer, R. P. (1960). In The Intestinal Tract, pp. 309-330. Thomas, Springfield, Ill. Symons, L. E. A, and Fairbairn, D. (1962). 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