{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T19:50:08Z","timestamp":1757620208543,"version":"3.44.0"},"reference-count":26,"publisher":"BMJ","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"published-print":{"date-parts":[[1974,3]]},"abstract":"<jats:p>In rats both rifamycin-SV and flavaspidic acid impaired the disappearance of bromsulphthalein (BSP) from plasma. The addition of rifamycin-SV to rat liver supernatant containing BSP did not displace BSP from ligandin or Z protein unless it was added in very high concentration. In similar studies <jats:italic>in vitro<\/jats:italic> with flavaspidic acid, BSP was displaced from Z protein even at low concentrations of flavaspidic acid, whereas there was only a minor effect on the binding to ligandin.<\/jats:p>\n               <jats:p>The intravenous administration of rifamycin to Gunn rats raised the plasma bilirubin concentration slightly after 30 minutes whereas flavaspidic acid was without significant effect. At 30 minutes the amount of bilirubin in the liver was markedly reduced by rifamycin and moderately depressed by flavaspidic acid. Although both the rifamycin and flavaspidic acid lowered the bilirubin in cell sap, the main effect of flavaspidic acid appeared to be on binding to Z protein. Rifamycin reduced the amount bound to both ligandin and Z protein. It is suggested that flavaspidic acid acted on the hepatic uptake of bilirubin and BSP predominantly by competing for binding to Z protein. Rifamycin-SV acted at a different site, probably blocking uptake at the plasma membrane.<\/jats:p>","DOI":"10.1136\/gut.15.3.220","type":"journal-article","created":{"date-parts":[[2007,9,18]],"date-time":"2007-09-18T17:41:31Z","timestamp":1190137291000},"page":"220-226","source":"Crossref","is-referenced-by-count":19,"title":["Sites of competition in the selective hepatic uptake of rifamycin-SV, flavaspidic acid, bilirubin, and bromsulphthalein"],"prefix":"10.1136","volume":"15","author":[{"given":"S.","family":"Kenwright","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. J.","family":"Levi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1974,3,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1016\/S0016-5085(19)34489-0","article-title":"The effect of rifamycin SV on bile pigment excretion in rats","volume":"49","author":"Acocella, G.; Billing, B.H.","year":"1965","unstructured":"Acocella, G., and Billing, B. H. (1965). The effect of rifamycin SV on bile pigment excretion in rats. Gastroenterology, 49, 526-530.","journal-title":"Gastroenterology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1016\/S0016-5085(19)34488-9","article-title":"The effect of an intravenous infusion of rifamycin SV on the excretion of bilirubin. bromsulphalein, and indocyanine green in man","volume":"49","author":"Acocella, G.; Nicolis, F.B.; Tenconi, L.T.","year":"1965","unstructured":"Acocella, G., Nicolis, F. B., and Tenconi, L. T. (1965). The effect of an intravenous infusion of rifamycin SV on the excretion of bilirubin. bromsulphalein, and indocyanine green in man. Gastroenterology, 49, 521-525.","journal-title":"Gastroenterology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1016\/S0016-5085(19)33296-2","article-title":"Defective bromosulfophthalein clearance in patients with constitutional hepatic dysfunction (Gilbert's syndrome)","volume":"63","author":"Berk, P.D.; Blaschke, T.F.; Waggoner, J.G.","year":"1972","unstructured":"Berk, P. D., Blaschke, T. F., and Waggoner, J. G. (1972). Defective bromosulfophthalein clearance in patients with constitutional hepatic dysfunction (Gilbert's syndrome). Gastroenterology, 63, 472-481.","journal-title":"Gastroenterology"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1042\/cs0450505","article-title":"Influence of albumin on the hepatic uptake of unconjugated bilirubin","volume":"45","author":"Bloomer, J.R.; Berk, P.D.; Vergalla, J.; Berlin, N.I.","year":"1973","unstructured":"Bloomer, J. R., Berk, P. D., Vergalla, J., and Berlin, N. I. (1973). Influence of albumin on the hepatic uptake of unconjugated bilirubin. Clin. Sci., 45, 505-516.","journal-title":"Clin. Sci"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1136\/gut.13.5.366","article-title":"Effect of rifampicin on liver function in man","volume":"13","author":"Capelle, P.; Dhumeaux, D.; Mora, M.; Feldmann, G.; Berthelot, P.","year":"1972","unstructured":"Capelle, P., Dhumeaux, D., Mora, M., Feldmann, G., and Berthelot, P. (1972). Effect of rifampicin on liver function in man. Gut, 13, 366-371.","journal-title":"Gut"},{"key":"ref_6","first-page":"118","article-title":"Clinical studies with a new rifamycin derivative","volume":"9","author":"Cohn, H.D.","year":"1969","unstructured":"Cohn, H. D. (1969). Clinical studies with a new rifamycin derivative. J. clin. Pharmacol., 9, 118-125.","journal-title":"J. clin. Pharmacol"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"665","DOI":"10.3181\/00379727-124-31819","article-title":"Binding of sulfobromophthalein sodium (BSP) and other organic anions by isolated hepatic cell plasma membranes in vitro","volume":"124","author":"Cornelius, C.E.; Ben-Ezzer, J.; Arias, I.M.","year":"1967","unstructured":"Cornelius, C. E., Ben-Ezzer, J., and Arias, I. M. (1967). Binding of sulfobromophthalein sodium (BSP) and other organic anions by isolated hepatic cell plasma membranes in vitro. Proc. Soc. exp. Biol. (N. Y.), 124, 665-667.","journal-title":"Proc. Soc. exp. Biol. (N. Y.)"},{"key":"ref_8","first-page":"291","article-title":"Congenital photosensitivity and hyperbilirubinemia in Southdown sheep in the United States","volume":"29","author":"Cornelius, C.E.; Gronwall, R.R.","year":"1968","unstructured":"Cornelius, C. E., and Gronwall, R. R. (1968). Congenital photosensitivity and hyperbilirubinemia in Southdown sheep in the United States. Amer. J. vet. Res., 29, 291-295.","journal-title":"Amer. J. vet. Res"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1016\/S0002-9343(70)80126-7","article-title":"Recent advances in bilirubin formation, transport, metabolism and excretion","volume":"49","author":"Fleischner, G.; Arias, I.M.","year":"1970","unstructured":"Fleischner, G., and Arias, I. M. (1970). Recent advances in bilirubin formation, transport, metabolism and excretion. Amer. J. Med., 49, 576-589.","journal-title":"Amer. J. Med"},{"key":"ref_10","first-page":"851","article-title":"The hepatic uptake and excretion of sulfobromophthalein and bilirubin","volume":"92","author":"Goresky, C.A.","year":"1965","unstructured":"Goresky, C. A. (1965). The hepatic uptake and excretion of sulfobromophthalein and bilirubin. Canad. med. Ass. J., 92, 851-857.","journal-title":"Canad. med. Ass. J"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/S0016-5085(19)34259-3","article-title":"Interference with bile pigment uptake in the liver by flavaspidic acid","volume":"53","author":"Hammaker, L.; Schmid, R.","year":"1967","unstructured":"Hammaker, L., and Schmid, R. (1967). Interference with bile pigment uptake in the liver by flavaspidic acid. Gastroenterology, 53, 31-37.","journal-title":"Gastroenterology"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1042\/bj1030316","article-title":"The isolation of carcinogen binding protein from livers of rats given 4 dimethylaminoazobenzene","volume":"103","author":"Ketterer, B.; Ross-Mansell, P.; Whitehead, J.K.","year":"1967","unstructured":"Ketterer, B., Ross-Mansell, P., and Whitehead, J. K. (1967). The isolation of carcinogen binding protein from livers of rats given 4 dimethylaminoazobenzene. Biochem. J., 103, 316-324.","journal-title":"Biochem. J"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1016\/0041-3879(71)90041-9","article-title":"Toxicity from rifampicin plus isoniazid and rifampicin plus ethambutol therapy","volume":"52","author":"Lees, A.W.; Allan, G.W.; Smith, J.; Tyrrell, W.F.; Fallon, R.J.","year":"1971","unstructured":"Lees, A. W., Allan, G. W., Smith, J., Tyrrell, W. F., and Fallon, R. J. (1971). Toxicity from rifampicin plus isoniazid and rifampicin plus ethambutol therapy. Tubercle, 52, 182-190.","journal-title":"Tubercle"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2156","DOI":"10.1172\/JCI106182","article-title":"Two hepatic cytoplasmic protein fractions, Y and Z, and their possible role in the hepatic uptake of bilirubin, sulfobromophthalein, and other anions. J. clin","volume":"48","author":"Levi, A.J.; Gatmaitan, Z.; Arias, I.M.","year":"1969","unstructured":"Levi, A. J., Gatmaitan, Z., and Arias, I. M. (1969). Two hepatic cytoplasmic protein fractions, Y and Z, and their possible role in the hepatic uptake of bilirubin, sulfobromophthalein, and other anions. J. clin. Invest., 48, 2156-2167.","journal-title":"Invest"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1136","DOI":"10.1056\/NEJM197011192832104","article-title":"Deficiency of hepatic organic anion-binding protein, impaired organic anion uptake by liver (and 'physiologic' jaundice in newborn monkeys","volume":"283","author":"Levi, A.J.; Gatmaitan, Z.; Arias, I.M.","year":"1970","unstructured":"Levi, A. J., Gatmaitan, Z., and Arias, I. M. (1970). Deficiency of hepatic organic anion-binding protein, impaired organic anion uptake by liver (and 'physiologic' jaundice in newborn monkeys. New Engl. J. Med., 283, 1136-1139.","journal-title":"New Engl. J. Med"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"652","DOI":"10.1016\/0003-2697(72)90080-2","article-title":"Determination of bilirubin in liver homogenate","volume":"50","author":"Mertens, B.B.E.; Van de Vijver, N.; Heirwegh, K.","year":"1972","unstructured":"Mertens, B. B. E., Van de Vijver, N., and Heirwegh, K. (1972). Determination of bilirubin in liver homogenate. Analyt. Biochem., 50, 652-655.","journal-title":"Analyt. Biochem"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"955","DOI":"10.3181\/00379727-133-34603","article-title":"Bilirubin\"IC tarnover studies in normal and mutant Southdown sheep with congenital hyperbilirubinemia","volume":"133","author":"Mia, A.S.; Gronwall, R.R.; Cornelius, C.E.","year":"1970","unstructured":"Mia, A. S., Gronwall, R. R., and Cornelius, C. E. (1970). Bilirubin\"IC tarnover studies in normal and mutant Southdown sheep with congenital hyperbilirubinemia. Proc. Soc. Exp. Biol. (N. Y.), 133, 955-959.","journal-title":"Proc. Soc. Exp. Biol. (N. Y.)"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1542\/peds.35.6.925","article-title":"Plasma bilirubin determination in the newborn infant","volume":"35","author":"Michaelsson, M.; Nosslin, B.; Sjolin, S.","year":"1965","unstructured":"Michaelsson, M., Nosslin, B., and Sjolin, S. (1965). Plasma bilirubin determination in the newborn infant. Pediatrics, 35, 925-931.","journal-title":"Pediatrics"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1016\/0006-291X(72)90931-X","article-title":"The binding of fatty acids to cytoplasmic proteins: binding to Z protein in liver and other tissues of the rat","volume":"47","author":"Mishkin, S.; Stein, L.; Gatmaitan, Z.; Arias, I.M.","year":"1972","unstructured":"Mishkin, S., Stein, L., Gatmaitan, Z., and Arias, I. M. (1972). The binding of fatty acids to cytoplasmic proteins: binding to Z protein in liver and other tissues of the rat. Biochem. biophys. Res. Commun., 47, 997-1003.","journal-title":"Biochem. biophys. Res. Commun"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"4813","DOI":"10.1021\/bi00840a024","article-title":"Isolation and properties of cortisol metabolite binding proteins of rat liver cytosol","volume":"8","author":"Morey, K.S.; Litwack, G.","year":"1969","unstructured":"Morey, K. S., and Litwack, G. (1969). Isolation and properties of cortisol metabolite binding proteins of rat liver cytosol. Biochemistry, 8, 4813-4821.","journal-title":"Biochemistry"},{"issue":"69","key":"ref_21","first-page":"206","article-title":"Bromsulphalein retention and jaundice due to unconjugated bilirubin following treatment with male fern extract","volume":"15","author":"Nosslin, B.","year":"1963","unstructured":"Nosslin, B. (1963). Bromsulphalein retention and jaundice due to unconjugated bilirubin following treatment with male fern extract. Scand. J. clin. Lab. Invest., 15, Suppl. 69, 206-212.","journal-title":"Scand. J. clin. Lab. Invest"},{"key":"ref_22","first-page":"891","article-title":"The effect of phloroglucinol derivatives from male fern on dye excretion by the liver in the rabbit and rat","volume":"65","author":"Nosslin, B.; Morgan, E.H.","year":"1965","unstructured":"Nosslin, B., and Morgan, E. H. (1965). The effect of phloroglucinol derivatives from male fern on dye excretion by the liver in the rabbit and rat. J. Lab. clin. Med., 65, 891-902.","journal-title":"J. Lab. clin. Med"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1111\/j.1749-6632.1970.tb37009.x","article-title":"Kinetics of indocyanine green removal from the blood","volume":"170","author":"Paumgartner, G.; Probst, P.; Kraines, R.; Leevy, C.M.","year":"1970","unstructured":"Paumgartner, G., Probst, P., Kraines, R., and Leevy, C. M. (1970). Kinetics of indocyanine green removal from the blood. Ann. N.Y. Acad. Sci., 170, 134-147.","journal-title":"Ann. N. Y. Acad. Sci"},{"key":"ref_24","first-page":"467","article-title":"Spectrophotometric and bacteriologic determination of rifampicine in biological products","volume":"18","author":"Pgunescu, E.; Stoinescu, M.","year":"1969","unstructured":"Pgunescu, E., and Stoinescu, M. (1969). Spectrophotometric and bacteriologic determination of rifampicine in biological products. (Rumanian). Ftiziologia, 18, 467-470.","journal-title":"(Rumanian). Ftiziologia"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1073\/pnas.64.1.168","article-title":"Organic anion-binding protein in rat liver: drug induction and its physiologic consequence","volume":"64","author":"Reyes, H.; Levi, A.J.; Gatmaitan, Z.; Arias, I.M.","year":"1969","unstructured":"Reyes, H., Levi, A. J., Gatmaitan, Z., and Arias, I. M. (1969). Organic anion-binding protein in rat liver: drug induction and its physiologic consequence. Proc. nat. Acad. Sci. (Wash.), 64, 168-170.","journal-title":"Proc. nat. Acad. Sci. (Wash.)"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/0009-8981(71)90367-6","article-title":"Determination of bilirubin in liver homogenates and serum with diazotized p-iodoaniline","volume":"31","author":"Van Roy, F.P.; Meuwissen, J.A.T.P.; De Meuter, F.; Heirwegh, K.P.M.","year":"1971","unstructured":"Van Roy, F. P., Meuwissen, J. A. T. P., De Meuter, F., and Heirwegh, K. P. M. (1971). Determination of bilirubin in liver homogenates and serum with diazotized p-iodoaniline. Clin. chim. Acta, 31, 109-118.","journal-title":"Clin. chim. Acta"}],"container-title":["Gut"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/gut.15.3.220","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T05:56:06Z","timestamp":1757310966000},"score":1,"resource":{"primary":{"URL":"https:\/\/gut.bmj.com\/lookup\/doi\/10.1136\/gut.15.3.220"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1974,3]]},"references-count":26,"journal-issue":{"issue":"3","published-online":{"date-parts":[[1974,3,1]]},"published-print":{"date-parts":[[1974,3]]}},"alternative-id":["10.1136\/gut.15.3.220"],"URL":"https:\/\/doi.org\/10.1136\/gut.15.3.220","relation":{},"ISSN":["0017-5749","1468-3288"],"issn-type":[{"type":"print","value":"0017-5749"},{"type":"electronic","value":"1468-3288"}],"subject":[],"published":{"date-parts":[[1974,3]]}}}