{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T19:05:04Z","timestamp":1783019104769,"version":"3.54.6"},"reference-count":13,"publisher":"BMJ","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"published-print":{"date-parts":[[1974,5]]},"abstract":"<jats:p>Liver iron concentration has been determined chemically in 154 liver biopsies and the findings compared with the routine histological assessment of stainable parenchymal iron, performed by an independent observer. There was a significant correlation between liver iron concentration and histochemical grading but the relationship did not have a normal linear form. Absence of stainable iron corresponded to liver iron concentrations below the mean value for control male subjects (77 \u03bcg\/100 mg dry liver). In general grade 1 siderosis corresponded to liver iron concentrations in the upper part of the control range and grade 2 siderosis to marginally elevated values. The transition from grade 2 to grade 3 (submaximal) siderosis represented a sharp increase in liver iron concentration and as grade 3 siderosis corresponded to a wide range of chemical values it is also the most difficult histochemical grade to interpret in quantitative terms. Grade 4 siderosis invariably indicated heavy iron excess.<\/jats:p>\n               <jats:p>There was a close correlation between liver iron concentration and measurements of total body storage iron obtained by quantitative phlebotomy in patients with idiopathic haemochromatosis and by determination of DTPA-chelatable body iron in a variety of iron-loading disorders.<\/jats:p>","DOI":"10.1136\/gut.15.5.411","type":"journal-article","created":{"date-parts":[[2007,9,18]],"date-time":"2007-09-18T17:41:31Z","timestamp":1190137291000},"page":"411-415","source":"Crossref","is-referenced-by-count":75,"title":["Liver iron concentration, stainable iron, and total body storage iron"],"prefix":"10.1136","volume":"15","author":[{"given":"Michael","family":"Barry","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[1974,5,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1136\/gut.10.9.697","article-title":"Differential ferrioxamine test in haemochromatosis and liver disease","volume":"10","author":"Barry, M.; Cartei, G.C.; Sherlock, S.","year":"1969","unstructured":"Barry, M., Cartei, G. C., and Sherlock, S. (1969). Differential ferrioxamine test in haemochromatosis and liver disease. Gut, 10, 697-704.","journal-title":"Gut"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1136\/gut.11.11.891","article-title":"Quantitative measurement of iron stores with diethylenetriamine penta-acetic acid","volume":"11","author":"Barry, M.; Cartei, G.C.; Sherlock, S.","year":"1970","unstructured":"Barry, M., Cartei, G. C., and Sherlock, S. (1970). Quantitative measurement of iron stores with diethylenetriamine penta-acetic acid. Gut, 11, 891-898.","journal-title":"Gut"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/S0140-6736(71)90838-5","article-title":"Measurement of liver iron concentration in needle-biopsy specimens","volume":"1","author":"Barry, M.; Sherlock, S.","year":"1971","unstructured":"Barry, M., and Sherlock, S. (1971). Measurement of liver iron concentration in needle-biopsy specimens. Lancet, 1, 100-103.","journal-title":"Lancet"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1093\/ajcn\/23.3.358","article-title":"Hepatic storage iron concentrations in different population groups","volume":"23","author":"Charlton, R.W.; Hawkins, D.M.; Mavor, W.O.; Bothwell, T.H.","year":"1970","unstructured":"Charlton, R. W., Hawkins, D. M., Mavor, W. O., and Bothwell, T. H. (1970). Hepatic storage iron concentrations in different population groups. Amer. J. clin. Nutr., 23, 358-370.","journal-title":"Amer. J. clin. Nutr"},{"key":"ref_5","first-page":"348","article-title":"Liver and tissue iron","volume":"77","author":"MacDonald, R.A.; Pechet, G.S.","year":"1964","unstructured":"MacDonald, R. A., and Pechet, G. S. (1964). Liver and tissue iron. Arch. Path., 77, 348-353.","journal-title":"Arch. Path"},{"key":"ref_6","first-page":"543","article-title":"Tissue iron and hemochromatosis","volume":"84","author":"MacDonald, R.A.","year":"1967","unstructured":"MacDonald, R. A. (1967). Tissue iron and hemochromatosis. Arch. Path., 84, 543-551.","journal-title":"Arch. Path"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1136\/jcp.16.2.101","article-title":"Iron storage in human disease: fractionation of hepatic and splenic iron into ferritin and haemosiderin with histochemical correlations","volume":"16","author":"Morgan, E.H.; Walters, M.N.I.","year":"1963","unstructured":"Morgan, E. H., and Walters, M. N. I. (1963). Iron storage in human disease: fractionation of hepatic and splenic iron into ferritin and haemosiderin with histochemical correlations. J. clin. Path., 16, 101-107.","journal-title":"J. clin. Path"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1002\/path.1700840107","article-title":"Hepatic pathology in relatives of patients with haemochromatosis","volume":"84","author":"Scheuer, P.J.; Williams, R.; Muir, A.R.","year":"1962","unstructured":"Scheuer, P. J., Williams, R., and Muir, A. R. (1962). Hepatic pathology in relatives of patients with haemochromatosis. J. Path. Bact., 84, 53-64.","journal-title":"J. Path. Bact"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1016\/S0021-9258(18)66085-5","article-title":"The relationship between ferritin and hemosiderin in rabbits and man","volume":"204","author":"Shoden, A.; Gabrio, B.W.; Finch, C.A.","year":"1953","unstructured":"Shoden, A., Gabrio, B. W., and Finch, C. A. (1953). The relationship between ferritin and hemosiderin in rabbits and man. J. biol. Chem., 204, 823-830.","journal-title":"J. biol. Chem"},{"key":"ref_10","volume-title":"Cytoand histochemistry of the liver. In The Liver: Morphology, Biochemistry and Physiology","volume":"1","author":"Wachstein, M.","year":"1963","unstructured":"Wachstein, M. (1963). Cytoand histochemistry of the liver. In The Liver: Morphology, Biochemistry and Physiology, edited by C. Rouiller, vol. 1. Academic Press, New York."},{"key":"ref_11","first-page":"10111014","article-title":"Relationship of hepatic iron concentration to histochemical grading and to total chelatable body iron in conditions associated with iron overload","volume":"12","author":"Walker, R.J.; Miller, J.P.G.; Dymock, L.W.; Shilkin, K.B.; Williams, R.","year":"1971","unstructured":"Walker, R. J., Miller, J. P. G., Dymock, L. W., Shilkin, K. B., and Williams, R. (1971). Relationship of hepatic iron concentration to histochemical grading and to total chelatable body iron in conditions associated with iron overload. Gut, 12, 10111014.","journal-title":"Gut"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1136\/jcp.21.1.35","article-title":"Significance for the diagnosis of iron overload of histochemical and chemical iron in the liver of control subjects","volume":"21","year":"1968","unstructured":"Weinfeld, A., Lundin, P., and Lundvall, 0. (1968). Significance for the diagnosis of iron overload of histochemical and chemical iron in the liver of control subjects. J. clin. Path., 21, 35-40.","journal-title":"J. clin. Path"},{"key":"ref_13","first-page":"275","article-title":"have suggested that the storage of liver in several widely used fixative solutions is associated with significant loss of iron from the specimen. To check this important point three wedge biopsies and one necropsy specimen, all of which had been previously analysed in thefreshstate, werere-analysed aftervarying periods of preservation in 10% formalinphysiological saline solution. The results (table) do not indicate any significant iron loss over periods of storage ranging from five to 18 months in this particular medium which is routinely used in this hospital","volume":"50","author":"Eijk, Wiltink, Bos; chim, Goosens (Clin.","year":"1974","unstructured":"Eijk,Wiltink,Bos, and Goosens (Clin. chim. Acta, 50, 1974, pp. 275-281) have suggested that the storage of liver in several widely used fixative solutions is associated with significant loss of iron from the specimen. To check this important point three wedge biopsies and one necropsy specimen, all of which had been previously analysed in thefreshstate, werere-analysed aftervarying periods of preservation in 10% formalinphysiological saline solution. The results (table) do not indicate any significant iron loss over periods of storage ranging from five to 18 months in this particular medium which is routinely used in this hospital.","journal-title":"Acta"}],"container-title":["Gut"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/gut.15.5.411","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T05:30:13Z","timestamp":1757309413000},"score":1,"resource":{"primary":{"URL":"https:\/\/gut.bmj.com\/lookup\/doi\/10.1136\/gut.15.5.411"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1974,5]]},"references-count":13,"journal-issue":{"issue":"5","published-online":{"date-parts":[[1974,5,1]]},"published-print":{"date-parts":[[1974,5]]}},"alternative-id":["10.1136\/gut.15.5.411"],"URL":"https:\/\/doi.org\/10.1136\/gut.15.5.411","relation":{},"ISSN":["0017-5749","1468-3288"],"issn-type":[{"value":"0017-5749","type":"print"},{"value":"1468-3288","type":"electronic"}],"subject":[],"published":{"date-parts":[[1974,5]]}}}