{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T08:52:03Z","timestamp":1765615923635,"version":"3.48.0"},"reference-count":20,"publisher":"BMJ","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"published-print":{"date-parts":[[1978,4]]},"abstract":"<jats:p>\n                    Subfractions of fraction 9, obtained from a peptic-tryptic-pancreatinic digest of wheat gliadin, were subjected to\n                    <jats:italic>in vitro<\/jats:italic>\n                    mucosal digestion and the filtrates examined for residual peptides. Small-intestinal mucosa from four groups of individuals were studied\u2014eight patients with coeliac disease in remission; eight healthy controls; nine first degree relatives of patients with coeliac disease, and six children with recurrent diarrhoea investigated for possible coeliac disease, but in whom the diagnosis was excluded. The highest amounts of residual peptides (measured by scanning densitometer) were detected after digestion with mucosa from patients with coeliac disease and the lowest amounts with the control groups. The results obtained with the group of relatives fell between those of the coeliac disease and control groups, while the recurrent diarrhoea group overlapped the relatives and controls. The residual peptides were derived chiefly from the B-type subfractions of subfractions 1 and 2, obtained by ion-exchange chromatography of fraction 9. These subfractions are rich in glutamine\/glutamic acid and proline and have a molecular weight (apparent) of not greater than 1500 Daltons. The results lend further support to the hypothesis of an enzyme deficiency in coeliac disease. A partial enzyme deficiency may exist in some first-degree relatives and in some children with recurrent diarrhoea but with histology of the small intestine within normal limits. HLA-B8 antigen is not correlated with this deficiency, but, when the two factors are associated, they could be related to the manifestation and severity of coeliac disease.\n                  <\/jats:p>","DOI":"10.1136\/gut.19.4.253","type":"journal-article","created":{"date-parts":[[2007,9,18]],"date-time":"2007-09-18T17:41:31Z","timestamp":1190137291000},"page":"253-259","source":"Crossref","is-referenced-by-count":27,"title":["Further evidence of a primary mucosal defect in coeliac disease"],"prefix":"10.1136","volume":"19","author":[{"given":"H. J.","family":"Cornell","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. J.","family":"Rolles","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1978,4,1]]},"reference":[{"key":"ref_1","first-page":"118","article-title":"Skin test for coeliac disease using a subfraction of gluten","volume":"1","author":"Anand, B.S.; Truelove, S.C.; Offord, R.E.","year":"1977","unstructured":"Anand, B. S., Truelove, S. C., and Offord, R. E. (1977). Skin test for coeliac disease using a subfraction of gluten. Lancet, 1, 118-120.","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1042\/bj0910222","article-title":"Estimation of the molecular weights of proteins by Sephadex gel-filtration","volume":"91","author":"Andrews, P.","year":"1964","unstructured":"Andrews, P. (1964). 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Investigation of possible intestinal peptidase deficiency in coeliac disease."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/0009-8981(73)90289-1","article-title":"The effect of gliadin peptides on rat-liver lysosomes in relation to the pathogenesis of coeliac disease","volume":"49","author":"Cornell, H.J.; Townley, R.R.W.","year":"1973","unstructured":"Cornell, H. J., and Townley, R. R. W. (1973b). The effect of gliadin peptides on rat-liver lysosomes in relation to the pathogenesis of coeliac disease. Clinica Chimica Acta, 49, 181-188.","journal-title":"Clinica Chimica Acta"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"862","DOI":"10.1136\/gut.15.11.862","article-title":"The toxicity of certain cereal proteins in coeliac disease","volume":"15","author":"Cornell, H.J.; Townley, R.R.W.","year":"1974","unstructured":"Cornell, H. J., and Townley, R. R. W. (1974). The toxicity of certain cereal proteins in coeliac disease. Gut, 15, 862-869.","journal-title":"Gut"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1602","DOI":"10.1172\/JCI106958","article-title":"Predominance of histocompatibility antigen HL-A8 in patients with gluten-sensitive enteropathy","volume":"51","author":"Falchuk, Z.M.; Rogentine, G.N.; Strober, W.","year":"1972","unstructured":"Falchuk, Z. M., Rogentine, G. N., and Strober, W. (1972). Predominance of histocompatibility antigen HL-A8 in patients with gluten-sensitive enteropathy. Journal of Clinical Investigation, 51, 1602-1605.","journal-title":"Journal of Clinical Investigation"},{"key":"ref_10","first-page":"339","article-title":"Non-specific cytotoxicity of wheat gliadin components towards cultured human cells","volume":"1","author":"Hudson, D.A.; Purdham, D.R.; Cornell, H.J.; Rolles, C.J.","year":"1976","unstructured":"Hudson, D. A., Purdham, D. R., Cornell, H. J., and Rolles, C. J. (1976). 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S., Rolles, C. J., and Arthur, L. J. H. (1976)."},{"key":"ref_14","series-title":"Coeliac Disease: Proceedings of the 2nd International Coeliac Symposium","first-page":"229","article-title":"Concordance for HL-A antigens in the parents of children with coeliac disease","author":"Rolles, C.J.; Mackintosh, P.","year":"1974","unstructured":"Rolles, C. J. and Mackintosh, P. (1974). Concordance for HL-A antigens in the parents of children with coeliac disease. In Coeliac Disease: Proceedings of the 2nd International Coeliac Symposium, pp. 229-231. Edited by W. T. J. M. Hekkens and A. S. Pefia. Stenfert Kroese: Leyden."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1309","DOI":"10.1136\/bmj.1.6021.1309","article-title":"Standardised approach to gluten challenge in diagnosing childhood coeliac disease","volume":"1","author":"Rolles, C.J.; McNeish, A.S.","year":"1976","unstructured":"Rolles, C. J., and McNeish, A. S. (1976). Standardised approach to gluten challenge in diagnosing childhood coeliac disease. British Medical Journal, 1, 1309-1311.","journal-title":"British Medical Journal"},{"key":"ref_16","first-page":"389","article-title":"Stimulation of lymphocytes from patients with coeliac disease by a subfraction of gluten","volume":"2","author":"Sikora, K.; Ahand, B.S.; Truelove, S.C.; Ciclitira, P.J.; Offord, R.E.","year":"1976","unstructured":"Sikora, K., Ahand, B. S., Truelove, S. C., Ciclitira, P. J., and Offord, R. E. (1976). Stimulation of lymphocytes from patients with coeliac disease by a subfraction of gluten. Lancet, 2, 389-391.","journal-title":"Lancet"},{"key":"ref_17","first-page":"204","article-title":"Chromatographic and Electrophoretic","volume":"1","author":"Smith, 1","year":"1969","unstructured":"Smith, 1. (1969), Chromatographic and Electrophoretic Techniques, Vol. 1, 3rd ed., pp. 204-205. Heinemann: London.","edition":"3rd ed","journal-title":"Techniques"},{"key":"ref_18","first-page":"162","article-title":"Histocompatibility antigens associated with adult coeliac disease","volume":"2","author":"Stokes, P.L.; Asquith, P.; Holmes, G.K.T.; Mackintosh, P.; Cooke, W.T.","year":"1972","unstructured":"Stokes, P. L., Asquith, P., Holmes, G. K. T., Mackintosh, P., and Cooke, W. T. (1972). Histocompatibility antigens associated with adult coeliac disease. Lancet, 2, 162-164.","journal-title":"Lancet"},{"key":"ref_19","first-page":"1363","article-title":"Toxicity of wheat gliadin fractions in coeliac disease","volume":"1","author":"Townley, R.R.W.; Bhathal, P.S.; Cornell, H.J.; Mitchell, J.D.","year":"1973","unstructured":"Townley, R. R. W., Bhathal, P. S., Cornell, H. J., and Mitchell, J. D. (1973). Toxicity of wheat gliadin fractions in coeliac disease. Lancet, 1, 1363-1364.","journal-title":"Lancet"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1016\/S0140-6736(76)90361-5","article-title":"An alternative mechanism for gluten toxicity in coeliac disease","volume":"1","author":"Weiser, M.M.; Douglas, A.P.","year":"1976","unstructured":"Weiser, M. M. and Douglas, A. P. (1976). An alternative mechanism for gluten toxicity in coeliac disease, Lancet, 1, 567-569.","journal-title":"Lancet"}],"container-title":["Gut"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/gut.19.4.253","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T08:48:56Z","timestamp":1765615736000},"score":1,"resource":{"primary":{"URL":"https:\/\/gut.bmj.com\/lookup\/doi\/10.1136\/gut.19.4.253"}},"subtitle":["<i>In vitro<\/i>\n                    mucosal digestion studies in coeliac patients in remission, their relatives, and control subjects"],"short-title":[],"issued":{"date-parts":[[1978,4]]},"references-count":20,"journal-issue":{"issue":"4","published-online":{"date-parts":[[1978,4,1]]},"published-print":{"date-parts":[[1978,4]]}},"alternative-id":["10.1136\/gut.19.4.253"],"URL":"https:\/\/doi.org\/10.1136\/gut.19.4.253","relation":{},"ISSN":["0017-5749","1468-3288"],"issn-type":[{"type":"print","value":"0017-5749"},{"type":"electronic","value":"1468-3288"}],"subject":[],"published":{"date-parts":[[1978,4]]}}}