{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T16:55:50Z","timestamp":1763744150153,"version":"3.45.0"},"reference-count":30,"publisher":"BMJ","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"published-print":{"date-parts":[[1979,4]]},"abstract":"<jats:p>\n                    In the peripheral blood of patients with Crohn's disease (CD) the numerical distribution of the three major B lymphocyte subsets was determined by the identification of surface immunoglobulins using F(ab)\n                    <jats:sub>2<\/jats:sub>\n                    -antibody fragments. T cell counts were also obtained and the number of null cells was calculated. Twenty-eight patients with Crohn's disease including 14 patients with previously untreated and very short-standing disease (group CD 1) and 14 patients with long-standing and\/or previous drug treated disease (group CD 2) were compared with 28 sex and age-matched normals as well as with 13 patients with acute inflammatory bowel disease (group D). Patients in group D and inactive patients of group CD 1 showed a significant absolute lymphocytosis due to an increase in both the three B cell subsets and the T cells, without changes in the null cells. While the proportion of T cells was normal, there was a significant relative B lymphocytosis and a relative null cytopenia in these patients. Active CD 1 patients, however, showed significantly lower absolute lymphocyte and T cell numbers. In group CD 2, there was a significant absolute lymphopenia caused by an equal decrease in B and T cells. Highly active CD 2 patients showed higher absolute null cell counts than inactive patients. With increasing disease duration there was a significant decrease of the relative and absolute B cell concentrations. The data obtained suggest that T and B cell populations in the peripheral blood are reduced in certain patients with Crohn's disease and that this occurs secondarily to activity of disease, chronicity of disease, and the effects of therapy.\n                  <\/jats:p>","DOI":"10.1136\/gut.20.4.261","type":"journal-article","created":{"date-parts":[[2007,9,18]],"date-time":"2007-09-18T17:41:31Z","timestamp":1190137291000},"page":"261-268","source":"Crossref","is-referenced-by-count":28,"title":["Immune status in Crohn's disease"],"prefix":"10.1136","volume":"20","author":[{"given":"I. O.","family":"Auer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"G\u00f6tz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Ziemer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Malchow","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Ehms","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1979,4,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1084","DOI":"10.4049\/jimmunol.119.3.1084","article-title":"F(ab')2 reagents are not required if goat, rather than rabbit, antibodies are used to detect human surface immunoglobuline","volume":"119","author":"Alexander, E.L.; Sander, S.K.","year":"1977","unstructured":"Alexander, E. L., and Sander, S. K. (1977). F(ab')2 reagents are not required if goat, rather than rabbit, antibodies are used to detect human surface immunoglobuline. Journal of Immunology, 119, 1084-1088.","journal-title":"Journal of Immunology"},{"key":"ref_2","article-title":"Immune status in Crohn's disease","author":"Auer, I.O.; Buschmann, C.; Ziemer, E.","year":"1978","unstructured":"Auer, I. O., Buschmann, C., and Ziemer, E. (1978a). Immune status in Crohn's disease."},{"key":"ref_3","first-page":"\"618","article-title":"Originally unimpaired primary cell mediated immunity in vitro","volume":"19","author":"Auer, I.O.; Wechsler, W.; Ziemer, E.; Malchow, H.; Sommer, H.","year":"1978","unstructured":"Originally unimpaired primary cell mediated immunity in vitro. Gut, 19, 618-626. Auer, I. O., Wechsler, W., Ziemer, E., Malchow, H., and Sommer, H. (1978b). Immune status in Crohn's disease. I. Leukocyte and lymphocyte subpopulations in peripheral blood. Scandinavian Journal of Gastroenterology, 13, 561-571.","journal-title":"Gut; Immune status in Crohn's disease"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1080\/00365521.1976.12097132","article-title":"Immunohistochemical characterization of local immunoglobulin formation in Crohn's disease of the ileum","volume":"11","author":"Baklien, K.; Brandtzaeg, P.","year":"1976","unstructured":"Baklien, K., and Brandtzaeg, P. (1976). Immunohistochemical characterization of local immunoglobulin formation in Crohn's disease of the ileum. Scandinavian Journal of Gastroenterology, 11, 447-457.","journal-title":"Scandinavian Journal of Gastroenterology"},{"key":"ref_5","first-page":"439","article-title":"Development of a Crohn's disease activity index","volume":"70","author":"Best, W.R.; Becktel, J.M.; Singleton, J.W.; Kern, F.","year":"1976","unstructured":"Best, W. R., Becktel, J. M., Singleton, J. W., and Kern, F. (1976). Development of a Crohn's disease activity index. National Co-operative Crohn's Disease Study. Gastroenterology, 70, 439-444.","journal-title":"National Co-operative Crohn's Disease Study. Gastroenterology"},{"key":"ref_6","first-page":"467","article-title":"The effect of acute and prolonged administration of prednisolone and ACTH on lymphocyte subpopulations","volume":"28","author":"Cooper, D.A.; Petts, V.; Luckhurst, E.; Penny, R.","year":"1977","unstructured":"Cooper, D. A., Petts, V., Luckhurst, E., and Penny, R. (1977). The effect of acute and prolonged administration of prednisolone and ACTH on lymphocyte subpopulations. Clinical and Experimental Immunology, 28, 467-473.","journal-title":"Clinical and Experimental Immunology"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1084\/jem.134.1.188","article-title":"Peyer's patches: an enriched source of precursors for IgA-producing immunocytes in the rabbit","volume":"134","author":"Craig, S.W.; Cebra, J.J.","year":"1971","unstructured":"Craig, S. W., and Cebra, J. J. (1971). Peyer's patches: an enriched source of precursors for IgA-producing immunocytes in the rabbit. Journal of Experimental Medicine, 134, 188-200.","journal-title":"Journal of Experimental Medicine"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1159\/000197797","article-title":"The distribution and enteric loss of 51Cr-labelled lymphocytes in normal subjects and in patients with coeliac disease and other disorders of the small intestine","volume":"14","author":"Douglas, A.P.; Weetman, A.P.; Haggith, J.W.","year":"1976","unstructured":"Douglas, A. P., Weetman, A. P., and Haggith, J. W. (1976). The distribution and enteric loss of 51Cr-labelled lymphocytes in normal subjects and in patients with coeliac disease and other disorders of the small intestine. Digestion, 14, 29-43.","journal-title":"Digestion"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/S0016-5085(76)80394-0","article-title":"Circulating antibodies to bovine albumin in ulcerative colitis and Crohn's disease. Characterization of the antibody response","volume":"70","author":"Falchuck, K.R.; Isselbacher, K.J.","year":"1976","unstructured":"Falchuck, K. R., and Isselbacher, K. J. (1976). Circulating antibodies to bovine albumin in ulcerative colitis and Crohn's disease. Characterization of the antibody response. Gastroenterology, 70, 5-8.","journal-title":"Gastroenterology"},{"key":"ref_10","first-page":"187","article-title":"Immune complexes in ulcerative colitis and Crohn's disease","volume":"29","author":"Hodgson, H.J.F.; Potter, B.J.; Jewell, D.P.","year":"1977","unstructured":"Hodgson, H. J. F., Potter, B. J., and Jewell, D. P. (1977). Immune complexes in ulcerative colitis and Crohn's disease. Clinical and Experimental Immunology, 29, 187-196.","journal-title":"Clinical and Experimental Immunology"},{"issue":"38","key":"ref_11","first-page":"91","article-title":"Lymphocyte subpopulations in chronic inflammatory bowel disease (Abstract)","volume":"11","author":"H0j, L.; Sorensen, S.F.","year":"1976","unstructured":"H0j, L., and Sorensen, S. F. (1976). Lymphocyte subpopulations in chronic inflammatory bowel disease (Abstract). Scandinavian Journal of Gastroenterology, 11, Suppl. 38, 91.","journal-title":"Scandinavian Journal of Gastroenterology"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1136\/gut.11.3.223","article-title":"IgM turnover in Crohn's disease","volume":"11","author":"Jensen, K.B.; Goltermann, N.; Jarnum, S.; Weeke, B.; Westergaard, H.","year":"1970","unstructured":"Jensen, K. B., Goltermann, N., Jarnum, S., Weeke, B., and Westergaard, H. (1970). IgM turnover in Crohn's disease. Gut, 11, 223-228.","journal-title":"Gut"},{"key":"ref_13","first-page":"785","article-title":"Lymphocytotoxic antibodies","volume":"1","author":"Lancet","year":"1976","unstructured":"Lancet (1976). Lymphocytotoxic antibodies (Editorial). 1, 785-786."},{"key":"ref_14","first-page":"939","article-title":"An appraisal of Fc receptors on human peripheral blood B and L lymphocytes","volume":"117","author":"Lobo, P.J.; Horwitz, D.A.","year":"1976","unstructured":"Lobo, P. J., and Horwitz, D. A. (1976). An appraisal of Fc receptors on human peripheral blood B and L lymphocytes. Journal ofImmunology, 117, 939-943.","journal-title":"Journal ofImmunology"},{"issue":"3","key":"ref_15","first-page":"105","article-title":"A model for local immunity. Ricerca in Clinica e in","volume":"6","author":"Robertson, S.M.; Cebra, J.J.","year":"1976","unstructured":"Robertson, S. M., and Cebra, J. J. (1976). A model for local immunity. Ricerca in Clinica e in Laboratorio, 6 (suppl. 3), 105-119.","journal-title":"Laboratorio"},{"key":"ref_16","article-title":"T and B lymphocytes and cutaneous anergy in inflammatory bowel disease","author":"Sachar, D.B.; Taub, R.N.; Rachamandar, K.; Meyers, S.; Forman, S.P.; Douglas, S.D.; Janowitz, H.D.","year":"1976","unstructured":"Sachar, D. B., Taub, R. N., Rachamandar, K., Meyers, S., Forman, S. P., Douglas, S. D., and Janowitz, H. D. (1976). T and B lymphocytes and cutaneous anergy in inflammatory bowel disease. Annals of the New York Academy","journal-title":"Annals of the New York Academy"},{"key":"ref_17","first-page":"65","article-title":"Immunologic functions of a subset of human Null cells","volume-title":"Clinical Evaluation of Immune Function in Man","author":"Schlossmann, S.F.; Chess, L.","year":"1976","unstructured":"Schlossmann, S. F., and Chess, L. (1976). Immunologic functions of a subset of human Null cells. In Clinical Evaluation of Immune Function in Man, pp. 65-95. Edited by S. D. Litwin, C. L. Christian, and G. W. Siskind."},{"key":"ref_18","first-page":"1055","article-title":"Grune and","volume":"54","author":"York. Schilssler, P.; Kruis, W.; Marget, W.","year":"1976","unstructured":"Grune and Stratton: New York. Schilssler, P., Kruis, W., and Marget, W. (1976). Lipoid-A-Antikorpertiter bei Morbus Crohn. Klinische Wochenschrift, 54, 1055-1056.","journal-title":"Klinische Wochenschrift; Lipoid-A-Antikorpertiter bei Morbus Crohn"},{"key":"ref_19","first-page":"38","article-title":"Critical evaluation of the value of immunofluorescence methods for the demonstration of immunoglobulins on the lymphocyte surface","volume":"59","author":"Sedlacek, H.H.; Dersjant, H.; Baudner, S.; Seiler, F.R.","year":"1976","unstructured":"Sedlacek, H. H., Dersjant, H., Baudner, S., and Seiler, F. R. (1976). Critical evaluation of the value of immunofluorescence methods for the demonstration of immunoglobulins on the lymphocyte surface. Behring Institute Mitteilungen, 59, 38-63.","journal-title":"Behring Institute Mitteilungen"},{"key":"ref_20","first-page":"673","article-title":"Inflammatory bowel disease. Cytophilic antibody and the cytotoxicity of lymphocytes for colonic cells in vitro","volume":"16","author":"Shorter, R.G.; Huizenga, K.A.; Spencer, R.J.; Aas, J.; Guy, S.K.","year":"1971","unstructured":"Shorter, R. G., Huizenga, K. A., Spencer, R. J., Aas, J., and Guy, S. K. (1971). Inflammatory bowel disease. Cytophilic antibody and the cytotoxicity of lymphocytes for colonic cells in vitro. American Journal of Digestive Diseases, 16, 673-679.","journal-title":"American Journal of Digestive Diseases"},{"key":"ref_21","first-page":"171","article-title":"In vitro studies of inflammatory bowel diseases. Surface receptors of the mononuclear cell required to lyse allogeneic colonic epithelial cells","volume":"70","author":"Stobo, J.D.; Tomasi, T.B.; Huizenga, K.A.; Spencer, R.J.; Shorter, R.","year":"1976","unstructured":"Stobo, J. D., Tomasi, T. B., Huizenga, K. A., Spencer, R. J., and Shorter, R. (1976). In vitro studies of inflammatory bowel diseases. Surface receptors of the mononuclear cell required to lyse allogeneic colonic epithelial cells. Gastroenterology, 70, 171-176.","journal-title":"Gastroenterology"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1136\/gut.16.11.847","article-title":"Peripheral blood and intestinal lymphocyte sub-populations in Crohn's disease","volume":"16","author":"Strickland, R.G.; Husby, G.; Black, W.C.; Williams, Jr, R.C.","year":"1975","unstructured":"Strickland, R. G., Husby, G., Black, W. C., and Williams, R. C., Jr (1975). Peripheral blood and intestinal lymphocyte sub-populations in Crohn's disease. Gut, 16, 847-853.","journal-title":"Gut"},{"key":"ref_23","article-title":"Peripheral blood T-and B-cells in chronic inflammatory bowel disease","author":"Strickland, R.G.; Korsmeyer, S.; Soltis, R.D.; Wilson, I.D.; Williams, Jr, R.C.","year":"1974","unstructured":"Strickland, R. G., Korsmeyer, S., Soltis, R. D., Wilson, I. D., and Williams, R. C., Jr (1974). Peripheral blood T-and B-cells in chronic inflammatory bowel disease."},{"key":"ref_24","article-title":"Escherichia coli 0: 14 and colon hemagglutinating antibodies in inflammatory bowel disease","author":"Thayer, Jr, W.R.; Brown, M.; Sangree, M.H.; Katz, J.; Hersh, T.","year":"1969","unstructured":"Thayer, W. R., Jr, Brown, M., Sangree, M. H., Katz, J., and Hersh, T. (1969). Escherichia coli 0: 14 and colon hemagglutinating antibodies in inflammatory bowel disease."},{"key":"ref_25","first-page":"942","article-title":"Salazopyrin and its effect on lymphocyte subpopulations and function (Abstract)","volume":"70","author":"Thayer, W.R.; Charland, C.; Field, C.","year":"1976","unstructured":"Thayer, W. R., Charland, C., and Field, C. (1976a). Salazopyrin and its effect on lymphocyte subpopulations and function (Abstract). Gastroenterology, 70, 942.","journal-title":"Gastroenterology"},{"year":"1976","author":"Thayer, Jr, W.R.; Charland, C.; Field, C.E.","key":"ref_26","unstructured":"Thayer, W. R., Jr, Charland, C., and Field, C. E. (1976b)."},{"key":"ref_27","first-page":"103","article-title":"Faecal flora of Crohn patients. Serological differentiation between Crohn's disease and ulcerative colitis","volume":"386","author":"Wensinck, F.","year":"1976","unstructured":"Wensinck, F. (1976). Faecal flora of Crohn patients. Serological differentiation between Crohn's disease and ulcerative colitis. In The Management of Crohn's Disease, edited by I. T. Weterman, A. S. Penia, and C. C. Booth, pp. 103-107. (Internat. Congress series, No. 386.) Amsterdam, Excerpta Medica.","journal-title":"The Management of Crohn's Disease; (Internat. Congress series"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1210","DOI":"10.4049\/jimmunol.114.4.1210","article-title":"IgG on lymphocyte surfaces; technical problems and the significance of a third cell population","volume":"114","author":"Winchester, R.J.; Fu, S.M.; Hoffmann, T.; Kunkel, H.G.","year":"1975","unstructured":"Winchester, R. J., Fu, S. M., Hoffmann, T., and Kunkel, H. G. (1975). IgG on lymphocyte surfaces; technical problems and the significance of a third cell population. Journal of Immunology, 114, 1210-1212.","journal-title":"Journal of Immunology"},{"key":"ref_29","article-title":"Lymphocyte surface markers: evidence for three subpopulations of Fc receptor bearing lymphocytes","volume-title":"Clinical Evaluation of Immune Function in","author":"Winchester, R.J.; Fu, S.M.; Wang, C.Y.; Hoffmann, T.; Kunkel, H.G.","year":"1976","unstructured":"Winchester, R. J., Fu, S. M., Wang, C. Y., Hoffmann, T., and Kunkel, H. G. (1976). Lymphocyte surface markers: evidence for three subpopulations of Fc receptor bearing lymphocytes. In Clinical Evaluation of Immune Function in"},{"key":"ref_30","first-page":"1","article-title":"Analyses of lymphocytes from patients with rheumatoid arthritis and systemic lupus erythematosus. Occurrence of interfering coldreactive antilymphocyte antibodies","author":"Man","year":"1974","unstructured":"Man, pp. 1-11. Edited by S. D. Litwin, C. L. Christian, and G. W. Siskind. Grune and Stratton: New York. Winchester, R. J., Winfield, J. B., Siegal, F., Wernet, P., Bentwich, Z., and Kunkel, H. (1974). Analyses of lymphocytes from patients with rheumatoid arthritis and systemic lupus erythematosus. Occurrence of interfering coldreactive antilymphocyte antibodies. Journal of Clinical","journal-title":"Journal of Clinical"}],"container-title":["Gut"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/gut.20.4.261","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T16:52:42Z","timestamp":1763743962000},"score":1,"resource":{"primary":{"URL":"https:\/\/gut.bmj.com\/lookup\/doi\/10.1136\/gut.20.4.261"}},"subtitle":["3. Peripheral blood B lymphocytes, enumerated by means of F(ab)\n                    <sub>2<\/sub>\n                    -antibody fragments, Null and T lymphocytes"],"short-title":[],"issued":{"date-parts":[[1979,4]]},"references-count":30,"journal-issue":{"issue":"4","published-online":{"date-parts":[[1979,4,1]]},"published-print":{"date-parts":[[1979,4]]}},"alternative-id":["10.1136\/gut.20.4.261"],"URL":"https:\/\/doi.org\/10.1136\/gut.20.4.261","relation":{},"ISSN":["0017-5749","1468-3288"],"issn-type":[{"type":"print","value":"0017-5749"},{"type":"electronic","value":"1468-3288"}],"subject":[],"published":{"date-parts":[[1979,4]]}}}