{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T07:30:16Z","timestamp":1782804616284,"version":"3.54.5"},"reference-count":35,"publisher":"BMJ","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"published-print":{"date-parts":[[1979,7]]},"abstract":"<jats:p>Using immunohistochemical techniques we studied duodenal biopsies from 18 patients with coeliac disease and 24 patients with normal duodenal morphology. We had access to antisera against the following gastrointestinal peptides: cholecystokinin (CCK), gastric inhibitory peptide (GIP), gastrin-17, glucagon-enteroglucagon, motilin, neurotensin, pancreatic peptide (PP), secretin, somatostatin, substance P and vasoactive intestinal peptide (VIP). The somatostatin, GIP, CCK, and glucagon cells were increased in number in coeliac disease. The number of motilin cells was slightly increased, while secretin cells were reduced. Cells storing gastrin-17, substance P, or neurotensin were rare in all patients regardless of diagnosis. No PP immunoreactive cells were found and VIP was localised to neurons only. In biopsies from patients having a mucosa with ridging of villi the number of the various endocrine cell types did not differ from that in the control group.<\/jats:p>","DOI":"10.1136\/gut.20.7.547","type":"journal-article","created":{"date-parts":[[2007,9,18]],"date-time":"2007-09-18T17:41:31Z","timestamp":1190137291000},"page":"547-552","source":"Crossref","is-referenced-by-count":53,"title":["Duodenal endocrine cells in adult coeliac disease."],"prefix":"10.1136","volume":"20","author":[{"given":"K","family":"Sj\u00f6lund","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Alumets","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N O","family":"Berg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"H\u00e5kanson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F","family":"Sundler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[1979,7,1]]},"reference":[{"key":"ref_1","author":"O'Dorisio, T.; of Endocrin, Div","year":"1976","unstructured":"T. O'Dorisio, Div. of Endocrin. and O'Dorisio et al. (1976)","journal-title":"and O'Dorisio et al"},{"key":"ref_2","author":"Metabolism, Ohio State Univ; Holst, Colombus J.; of Clin, Dept","year":"1974","unstructured":"Metabolism, Ohio State Univ., Colombus J. Holst, Dept. of Clin. Chem. Bispebjerg Holst and Aasted (1974)","journal-title":"Chem. Bispebjerg Holst and Aasted"},{"key":"ref_3","author":"Hospital, Copenhagen; Yanaihara, Denmark N.; Dept","year":"1978","unstructured":"Hospital, Copenhagen, Denmark N. Yanaihara, Dept. of Bioorganic Yanaihara et al. (1978)","journal-title":"of Bioorganic Yanaihara et al"},{"key":"ref_4","author":"Chemistry, College of Pharmacy; Shizuoka, Japan R.Carraway; of Phys., Dept; Lab","year":"1976","unstructured":"Chemistry, College of Pharmacy, Shizuoka, Japan R. Carraway, Dept. of Phys. and Lab. Carraway and Leeman of Human Reproduction, Harvard Med. (1976)","journal-title":"Carraway and Leeman of Human Reproduction, Harvard Med"},{"key":"ref_5","author":"Lab, R.E.Chance Lilly Res","year":"1976","unstructured":"R. E. Chance Lilly Res. Lab. Eli Lilly, Larsson et al. (1976)","journal-title":"Eli Lilly, Larsson et al"},{"key":"ref_6","author":"Fahrenkrug, J.; B, 0.","year":"1976","unstructured":"J. Fahrenkrug and 0. B. Schaffalitzky Fahrenkrug et al. (1976)","journal-title":"Schaffalitzky Fahrenkrug et al"},{"key":"ref_7","article-title":"Station de Phys","author":"Hospital, Bispebjerg; Copenhagen, Denmark M.P.Dubois","year":"1975","unstructured":"Bispebjerg Hospital, Copenhagen, Denmark M. P. Dubois, Station de Phys. de la Dubois (1975)"},{"key":"ref_8","author":"Agronomique, Nouzilly; Nilsson, France G.; Dept","year":"1975","unstructured":"Agronomique, Nouzilly, France G. Nilsson, Dept. of Pharm., Karolinska Nilsson et al. (1975)","journal-title":"of Pharm., Karolinska Nilsson et al"},{"key":"ref_9","article-title":"Sweden Same source as secretin antiserum Fahrenkrug and Schaffalitzky","author":"Inst., Stockholm","year":"1977","unstructured":"Inst., Stockholm, Sweden Same source as secretin antiserum Fahrenkrug and Schaffalitzky de Muckadell (1977)"},{"key":"ref_10","article-title":"The glucagon-enteroglucagon cells characteristically displayed a variable intensity of immunostaining. Their number was slightly increased in coeliac disease (Table 4). In some other mammalian species these cells seem to be identical with the cells storing GIP immunoreactivity (Alumets","author":"specimens","year":"1978","unstructured":"specimens. The glucagon-enteroglucagon cells characteristically displayed a variable intensity of immunostaining. Their number was slightly increased in coeliac disease (Table 4). In some other mammalian species these cells seem to be identical with the cells storing GIP immunoreactivity (Alumets et al., 1978). In man, however, only a proportion of the GIP immunoreactive cells displays glucagonenteroglucagon immunoreactivity.","journal-title":"et al; In man, however, only a proportion of the GIP immunoreactive cells displays glucagonenteroglucagon immunoreactivity"},{"key":"ref_11","first-page":"236","article-title":"The small intestine. In Dermatitis Herpetiformis (Major Problems in","volume":"4","author":"Alexander, J. 0","year":"1975","unstructured":"Alexander, J. 0. (1975). The small intestine. In Dermatitis Herpetiformis (Major Problems in Dermatology, vol. 4), p. 236 ff. Saunders: London.","journal-title":"Dermatology"},{"key":"ref_12","article-title":"Is GIP a glucagon cell constituent?","author":"Alumets, J.; HAkanson, R.; O'Dorisio, T.; Sjolund, K.; Sundler, F.","year":"1978","unstructured":"Alumets, J., HAkanson, R., O'Dorisio, T., Sjolund, K., and Sundler, F. (1978). Is GIP a glucagon cell constituent?"},{"key":"ref_13","first-page":"785","article-title":"Gut hormone profile in coeliac disease","volume":"1","author":"Besterman, H.S.; Bloom, S.R.; Sarson, D.L.; Blackburn, A.M.; Johnston, D.J.; Patel, H.R.; Stewart, J.S.; Modigliani, R.; Guerin, S.; Mallison, C.N.","year":"1978","unstructured":"Besterman, H. S., Bloom, S. R., Sarson, D. L., Blackburn, A. M., Johnston, D. J., Patel, H. R., Stewart, J. S., Modigliani, R., Guerin, S., and Mallison, C. N. (1978). Gut hormone profile in coeliac disease. Lancet, 1, 785-788.","journal-title":"Lancet"},{"key":"ref_14","first-page":"318","volume-title":"Fluorescence microscopic and microspectrofluorometric techniques for the cellular localization and characterization of biogenic amines: In Methods in Investigative and Diagnostic","author":"Vol, Endocrinologyby S.A.Berson; Bjorklund, A.; Faick, B.; Owman, C.","year":"1972","unstructured":"Bjorklund, A., Faick, B., and Owman, C. (1972). Fluorescence microscopic and microspectrofluorometric techniques for the cellular localization and characterization of biogenic amines: In Methods in Investigative and Diagnostic Endocrinology. Edited by S. A. Berson. Vol. I: The Thyroid and Biogenic Amines. Edited by J. E. Rall and I. J. Kopin, pp. 318-368. North-Holland: Amsterdam."},{"key":"ref_15","author":"Bloom, S.R.; Patel, H.R.; Johnston, D.I.","year":"1976","unstructured":"Bloom, S. R., Patel, H. R., and Johnston, D. I. (1976)."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"7035","DOI":"10.1016\/S0021-9258(17)32937-X","article-title":"Radioimmunoassay of neurotensin, a hypothalamic peptide","volume":"251","author":"Carraway, R.; Leeman, S.E.","year":"1976","unstructured":"Carraway, R., and Leeman, S. E. (1976). Radioimmunoassay of neurotensin, a hypothalamic peptide. Journal of Biological Chemistry, 251, 7035-7044.","journal-title":"Journal of Biological Chemistry"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1136\/gut.18.11.882","article-title":"Increased tissue concentrations of 5-hydroxytryptamine in the duodenal mucosa of patients with coeliac disease","volume":"18","author":"Challacombe, D.N.; Dawkins, P.D.; Baker, P.","year":"1977","unstructured":"Challacombe, D. N., Dawkins, P. D., and Baker, P. (1977). Increased tissue concentrations of 5-hydroxytryptamine in the duodenal mucosa of patients with coeliac disease. Gut, 18, 882-886.","journal-title":"Gut"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1136\/gut.18.5.373","article-title":"Enterochromaffin cells in the duodenal mucosa of children with coeliac disease","volume":"18","author":"Challacombe, D.N.; Robertson, K.","year":"1977","unstructured":"Challacombe, D. N., and Robertson, K. (1977). Enterochromaffin cells in the duodenal mucosa of children with coeliac disease. Gut, 18, 373-376.","journal-title":"Gut"},{"key":"ref_19","first-page":"1149","article-title":"Pancreozymin secretion is impaired in sprue (Abstract)","volume":"56","author":"Di Magno, E.P.; Go, V.L.W.; Summerskill, W.H.J.","year":"1969","unstructured":"Di Magno, E. P., Go, V. L. W., and Summerskill, W. H. J. (1969). Pancreozymin secretion is impaired in sprue (Abstract). Gastroenterology, 56, 1149.","journal-title":"Gastroenterology"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1016\/S0016-5085(76)80044-3","article-title":"Heptadecapeptide gastrin: Measurement in blood by specific radioimmunoassay","volume":"71","author":"Dockray, G.J.; Taylor, I.L.","year":"1976","unstructured":"Dockray, G. J., and Taylor, I. L. (1976). Heptadecapeptide gastrin: Measurement in blood by specific radioimmunoassay. Gastroenterology, 71, 971-977.","journal-title":"Gastroenterology"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1340","DOI":"10.1073\/pnas.72.4.1340","article-title":"Immunoreactive somatostatin is present in discrete cells of the endocrine pancreas","volume":"72","author":"Dubois, M.P.","year":"1975","unstructured":"Dubois, M. P. (1975). Immunoreactive somatostatin is present in discrete cells of the endocrine pancreas. Proceedings of the National Academy of Sciences, 72, 1340-1343.","journal-title":"Proceedings of the National Academy of Sciences"},{"key":"ref_22","author":"Fahrenkrug, J.; Schaffalitzky de Muckadell, 0","year":"1977","unstructured":"Fahrenkrug, J., and Schaffalitzky de Muckadell, 0. B. (1977)."},{"key":"ref_23","first-page":"1379","article-title":"Radioimmunoassay of vasoactive intestinal polypeptide (VIP) in plasma","volume":"89","unstructured":"Radioimmunoassay of vasoactive intestinal polypeptide (VIP) in plasma. Journal of Laboratory and Clinical Medicine, 89, 1379-1388.","journal-title":"Journal of Laboratory and Clinical Medicine"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"281","DOI":"10.3109\/00365517609055262","article-title":"Production and evaluation of antibodies for radio immunoassay of secretin","volume":"36","year":"1976","unstructured":"Fahrenkrug, J., Schaffalitzky de Muckadell, 0. B., and Rehfeld, J. F. (1976). Production and evaluation of antibodies for radio immunoassay of secretin. Scandinavian Journal of Clinical and Laboratory Investigation, 36, 281-287.","journal-title":"Scandinavian Journal of Clinical and Laboratory Investigation"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1530\/acta.0.0770715","article-title":"Production and evaluation of glucagon antibodies for radio immunoassay","volume":"77","author":"Hoist, J.J.; Aasted, B.","year":"1974","unstructured":"Hoist, J. J., and Aasted, B. (1974). Production and evaluation of glucagon antibodies for radio immunoassay. Acta Endocrinologica (Kbh), 77, 715-726.","journal-title":"Acta Endocrinologica (Kbh)"},{"key":"ref_26","author":"Larsson, L.I.; Sundler, F.; HAkanson, R.","year":"1976","unstructured":"Larsson, L. I., Sundler, F., and HAkanson, R. (1976)."},{"key":"ref_27","first-page":"991","article-title":"Gallbladder inertia and sluggish enterohepatic circulation of bile-salts in coeliac disease","volume":"1","author":"Low-Beer, T.S.; Heaton, K.W.; Heaton, S.T.; Read, A.E.","year":"1971","unstructured":"Low-Beer, T. S., Heaton, K. W., Heaton, S. T., and Read, A. E. (1971). Gallbladder inertia and sluggish enterohepatic circulation of bile-salts in coeliac disease. Lancet, 1, 991-994.","journal-title":"Lancet"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1111\/j.1748-1716.1975.tb05915.x","article-title":"Presence of substance P-like immunoreactivity in plasma from man and dog","volume":"94","author":"Nilsson, G.; Pernow, B.; Fischer, G.H.; Folkers, K.","year":"1975","unstructured":"Nilsson, G., Pernow, B., Fischer, G. H., and Folkers, K. (1975). Presence of substance P-like immunoreactivity in plasma from man and dog. Acta Physiologica Scandinavica, 94, 542-544.","journal-title":"Acta Physiologica Scandinavica"},{"key":"ref_29","first-page":"761","article-title":"Gastric inhibitory polypeptide (GIP). Intestinal distribution and stimulation by amino acids and medium-chain-triglycerides","volume":"21","author":"O'Dorisio, T.M.; Cataland, S.; Stevenson, M.; Mazzaferri, E.L.","year":"1976","unstructured":"O'Dorisio, T. M., Cataland, S., Stevenson, M., and Mazzaferri, E. L. (1976). Gastric inhibitory polypeptide (GIP). Intestinal distribution and stimulation by amino acids and medium-chain-triglycerides. American Journal of Digestive Diseases, 21, 761-765.","journal-title":"American Journal of Digestive Diseases"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1007\/BF01004561","article-title":"Bifunctional reagents as vapourand liquid-phase fixatives for immunhistochemistry","volume":"7","author":"Pearse, A.G.E.; Polak, J.M.","year":"1975","unstructured":"Pearse, A. G. E., and Polak, J. M. (1975). Bifunctional reagents as vapourand liquid-phase fixatives for immunhistochemistry. Histochemical Journal, 7, 179-186.","journal-title":"Histochemical Journal"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1016\/0016-5085(78)93307-3","article-title":"Cholecystokinin abnormalities in coeliac disease (Abstract)","volume":"74","author":"Polak, J.M.; Bloom, S.R.; McCrossan, M.V.; Timson, C.M.; Johnston, D.R.; Hudson, D.; Szelke, M.; Pearse, A.G.E.","year":"1978","unstructured":"Polak, J. M., Bloom, S. R., McCrossan, M. V., Timson, C. M., Johnston, D. R., Hudson, D., Szelke, M., and Pearse, A. G. E. (1978). Cholecystokinin abnormalities in coeliac disease (Abstract). Gastroenterology, 74, 1079.","journal-title":"Gastroenterology"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1136\/gut.14.11.870","article-title":"Secretin cells in coeliac disease","volume":"14","author":"Polak, J.M.; Pearse, A.G.E.; van Noorden, S.; Bloom, S.R.; Rossiter, M.A.","year":"1973","unstructured":"Polak, J. M., Pearse, A. G. E., van Noorden, S., Bloom, S. R., and Rossiter, M. A. (1973). Secretin cells in coeliac disease. Gut, 14, 870-874.","journal-title":"Gut"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1016\/0009-8981(76)90145-5","article-title":"A reliable method for generating antibodies against pancreozymin, secretin and gastrin","volume":"73","author":"Schlegel, W.; aRd Raptis, S.","year":"1977","unstructured":"Schlegel, W., aRd Raptis, S. (1977). A reliable method for generating antibodies against pancreozymin, secretin and gastrin. Clinica Chimica Acta, 73, 439-444.","journal-title":"Clinica Chimica Acta"},{"key":"ref_34","article-title":"Immunocytochemistry","author":"Sternberger, L.A.","year":"1974","unstructured":"Sternberger, L. A. (1974). Immunocytochemistry. PrenticeHall: New Jersey."},{"issue":"49","key":"ref_35","first-page":"195","article-title":"Characterization of various antisera against synthetic porcine motilin (Abstract)","volume":"13","author":"Yanaihara, N.; Sato, H.; Yanaihara, C.; Shimizu, G.; Mihara, S.; Mochizuki, T.; Yamaguchi, I.; Abe, K.; Itoh, Z.; Shin, H.; Imagawa, K.","year":"1978","unstructured":"Yanaihara, N., Sato, H., Yanaihara, C., Shimizu, G., Mihara, S., Mochizuki, T., Yamaguchi, I., Abe, K., Itoh, Z., Shin, H., and Imagawa, K. (1978). Characterization of various antisera against synthetic porcine motilin (Abstract). Scandinavian Journal of Gastroenterology, 13, suppl. 49, 195.","journal-title":"Scandinavian Journal of Gastroenterology"}],"container-title":["Gut"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/gut.20.7.547","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T03:23:47Z","timestamp":1764991427000},"score":1,"resource":{"primary":{"URL":"https:\/\/gut.bmj.com\/lookup\/doi\/10.1136\/gut.20.7.547"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1979,7]]},"references-count":35,"journal-issue":{"issue":"7","published-online":{"date-parts":[[1979,7,1]]},"published-print":{"date-parts":[[1979,7]]}},"alternative-id":["10.1136\/gut.20.7.547"],"URL":"https:\/\/doi.org\/10.1136\/gut.20.7.547","relation":{},"ISSN":["0017-5749","1468-3288"],"issn-type":[{"value":"0017-5749","type":"print"},{"value":"1468-3288","type":"electronic"}],"subject":[],"published":{"date-parts":[[1979,7]]}}}