{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T10:16:55Z","timestamp":1771064215487,"version":"3.50.1"},"reference-count":21,"publisher":"BMJ","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"published-print":{"date-parts":[[2004,9]]},"abstract":"<jats:p>\n                    <jats:bold>Background:<\/jats:bold>\n                    In coeliac disease (CD) patients, the dominant DQ2-\u0391-I-gliadin peptide recognised by CD4 T cells is contained within peptide sequence 57\u201373 (p57-73) of \u0391-gliadin. This peptide sequence is also located within a 33-mer protease resistant gliadin fragment and therefore is likely to play an important role in the pathogenesis of CD.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Aims:<\/jats:bold>\n                    Our aim was to determine whether a B cell epitope was present within the immunodominant T cell epitope of \u0391-gliadin and, if so, to elucidate its sequence and determine the importance of deamidation and\/or modification of the amino acid at position 65 for IgA binding.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Patients and methods:<\/jats:bold>\n                    A cohort of CD patients, disease controls, and healthy individuals were examined. Serum IgA antibodies to the native and modified p57-73 fragment of \u0391-gliadin were analysed using enzyme linked immunosorbent assays. Peptide scanning experiments were further used to elucidate the B cell epitope.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results and conclusion:<\/jats:bold>\n                    IgA antibodies to p57-73 were found in 29\/72 (40.2%) endomysial antibody positive patients, all of whom had CD. The peptide antibody appeared to be present when patients were on a diet containing gluten and declined on a gluten free diet. The p57-73 antibody was very specific for CD (98%) and had a sensitivity of 56%. The amino acid at position 65 was not important for IgA binding but was crucial for T cell recognition of p57-73. Pentapeptide PXPQP emerges as a potentially strong candidate for the IgA binding motif in this region of \u0391-gliadin. This study shows that a significant proportion of newly diagnosed CD patients have an antibody response to the immunodominant T cell epitope.\n                  <\/jats:p>","DOI":"10.1136\/gut.2003.032755","type":"journal-article","created":{"date-parts":[[2004,8,11]],"date-time":"2004-08-11T19:02:47Z","timestamp":1092250967000},"page":"1274-1278","source":"Crossref","is-referenced-by-count":16,"title":["IgA antibodies of coeliac disease patients recognise a dominant T cell epitope of \u0391-gliadin"],"prefix":"10.1136","volume":"53","author":[{"given":"E A L","family":"Bateman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B L","family":"Ferry","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Hall","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S A","family":"Misbah","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Anderson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P","family":"Kelleher","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[2004,8,11]]},"reference":[{"key":"2025121313002638000_53.9.1274.1","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2001.22123"},{"key":"2025121313002638000_53.9.1274.2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.2000.01312.x"},{"key":"2025121313002638000_53.9.1274.3","doi-asserted-by":"publisher","DOI":"10.1038\/73200"},{"key":"2025121313002638000_53.9.1274.4","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3083.1998.00397.x"},{"key":"2025121313002638000_53.9.1274.5","doi-asserted-by":"publisher","DOI":"10.1084\/jem.191.4.603"},{"key":"2025121313002638000_53.9.1274.6","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.17.10050"},{"key":"2025121313002638000_53.9.1274.7","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-4141(199910)29:10<3133::AID-IMMU3133>3.0.CO;2-G"},{"key":"2025121313002638000_53.9.1274.8","doi-asserted-by":"publisher","DOI":"10.1126\/science.1074129"},{"key":"2025121313002638000_53.9.1274.9","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1093\/ajcn\/69.3.354","volume":"69","year":"1999","journal-title":"Am J Clin Nutr"},{"key":"2025121313002638000_53.9.1274.10","first-page":"475","volume":"72","year":"1995","journal-title":"Cereal Chem"},{"key":"2025121313002638000_53.9.1274.11","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.1998.00722.x"},{"key":"2025121313002638000_53.9.1274.12","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1136\/gut.44.2.168","volume":"44","year":"1999","journal-title":"Gut"},{"key":"2025121313002638000_53.9.1274.13","doi-asserted-by":"crossref","first-page":"2023","DOI":"10.1093\/clinchem\/47.11.2023","volume":"47","year":"2001","journal-title":"Clin Chem"},{"key":"2025121313002638000_53.9.1274.14","doi-asserted-by":"crossref","unstructured":"Altman DG. Practical statistics for medical research. London, UK: Chapman and Hall, 1991.","DOI":"10.1201\/9780429258589"},{"key":"2025121313002638000_53.9.1274.15","doi-asserted-by":"publisher","DOI":"10.1126\/science.1077572"},{"key":"2025121313002638000_53.9.1274.16","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)02499-5"},{"key":"2025121313002638000_53.9.1274.17","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3083.2001.00869.x"},{"key":"2025121313002638000_53.9.1274.18","doi-asserted-by":"publisher","DOI":"10.1038\/nm840"},{"key":"2025121313002638000_53.9.1274.19","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(03)00387-1"},{"key":"2025121313002638000_53.9.1274.20","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119622"},{"key":"2025121313002638000_53.9.1274.21","doi-asserted-by":"crossref","unstructured":"Kasarda DD, Okita TW, Bernardin JE, et al. Nucleic acid (cDNA) and amino acid sequences of alpha-type gliadins from wheat (Triticum aestivum). Proc Natl Acal Sci USA 81:4712\u201316.","DOI":"10.1073\/pnas.81.15.4712"}],"container-title":["Gut"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/gut.2003.032755","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T21:00:32Z","timestamp":1765659632000},"score":1,"resource":{"primary":{"URL":"https:\/\/gut.bmj.com\/lookup\/doi\/10.1136\/gut.2003.032755"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,8,11]]},"references-count":21,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2004,8,11]]},"published-print":{"date-parts":[[2004,9]]}},"alternative-id":["10.1136\/gut.2003.032755"],"URL":"https:\/\/doi.org\/10.1136\/gut.2003.032755","relation":{},"ISSN":["0017-5749","1468-3288"],"issn-type":[{"value":"0017-5749","type":"print"},{"value":"1468-3288","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,8,11]]}}}