{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:21:11Z","timestamp":1791159671728,"version":"4.1.0"},"reference-count":33,"publisher":"BMJ","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"published-print":{"date-parts":[[2005,7]]},"abstract":"\n Background and aims:<\/jats:bold>\n Conflicting results exist about the presence of\n Mycobacterium avium<\/jats:italic>\n subspecies\n paratuberculosis<\/jats:italic>\n (MAP) specific IS\n 900<\/jats:italic>\n DNA in Crohn\u2019s disease (CD) tissues. Therefore, we examined IS\n 900<\/jats:italic>\n in a large number of gut samples from patients with CD (n\u200a=\u200a100) and ulcerative colitis (UC, n\u200a=\u200a100), and in non-inflamed control tissues (nIBD, n\u200a=\u200a100). We hypothesised that IS\n 900<\/jats:italic>\n DNA detection might be associated with distinct clinical phenotypic characteristics in CD.\n <\/jats:p>\n \n Methods:<\/jats:bold>\n The prevalence of MAP DNA in surgically resected tissues was examined using a mechanical-enzymatic disruption technique and nested IS\n 900<\/jats:italic>\n specific polymerase chain reaction (PCR). CD patients were stratified according to the criteria of the Vienna classification and other clinical characteristics.\n <\/jats:p>\n \n Results:<\/jats:bold>\n IS\n 900<\/jats:italic>\n PCR detection rate was significantly higher in CD tissue samples (52%) than in UC (2%) or nIBD (5%) specimens (p<0.0001). In CD patients, IS\n 900<\/jats:italic>\n DNA was detected in samples from both diseased small bowel (47%) as well as from the colon (61%). No firm association between MAP specific IS\n 900<\/jats:italic>\n detection rates and clinical phenotypic characteristics in CD could be established. However, corticosteroid medication constituted a factor which tended to have a negative influence on IS\n 900<\/jats:italic>\n DNA detection rates in CD (p<0.01).\n <\/jats:p>\n \n Conclusions:<\/jats:bold>\n The presence of MAP specific IS\n 900<\/jats:italic>\n DNA is a predominant feature of CD. Therapeutic intervention against MAP might represent a potential target for disease mitigation in Crohn\u2019s disease.\n <\/jats:p>","DOI":"10.1136\/gut.2004.045526","type":"journal-article","created":{"date-parts":[[2005,6,11]],"date-time":"2005-06-11T05:59:25Z","timestamp":1118469565000},"page":"944-949","source":"Crossref","is-referenced-by-count":147,"title":["High prevalence of\n Mycobacterium avium<\/i>\n subspecies\n paratuberculosis<\/i>\n IS\n 900<\/i>\n DNA in gut tissues from individuals with Crohn\u2019s 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