{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T03:49:26Z","timestamp":1782272966653,"version":"3.54.5"},"reference-count":58,"publisher":"BMJ","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:p>\n                    <jats:bold>Objective:<\/jats:bold>\n                    The transcriptional profile of gastric epithelial cell lines cocultured with\n                    <jats:italic>Helicobacter pylori<\/jats:italic>\n                    and the global gene expression of whole gastric mucosa has been described previously. We aimed to overcome limitations of previous studies by determining the effects of\n                    <jats:italic>H pylori<\/jats:italic>\n                    eradication on the transcriptome of purified human gastric epithelium using each patient as their own control.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Design:<\/jats:bold>\n                    Laser capture microdissection (LCM) was used to extract mRNA from paraffin-embedded antral epithelium from 10 patients with peptic ulcer disease, before and after\n                    <jats:italic>H pylori<\/jats:italic>\n                    eradication. mRNA was reverse transcribed and applied on to Affymetrix cDNA microarray chips customised for formalin-fixed tissue. Differentially expressed genes were identified and a subset validated by real-time polymerase chain reaction (PCR).\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results:<\/jats:bold>\n                    A total of 13 817 transcripts decreased and 9680 increased after\n                    <jats:italic>H pylori<\/jats:italic>\n                    eradication. Applying cut-off criteria (p&lt;0.02, fold-change threshold 2.5) reduced the sample to 98 differentially expressed genes. Genes detected included those previously implicated in\n                    <jats:italic>H pylori<\/jats:italic>\n                    pathophysiology such as interleukin 8, chemokine ligand 3, \u03b2 defensin and somatostatin, as well as novel genes such as GDDR (TFIZ1), chemokine receptors 7 and 8, and gastrokine.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusions:<\/jats:bold>\n                    LCM of archival specimens has enabled the identification of gastric epithelial genes whose expression is considerably altered after\n                    <jats:italic>H pylori<\/jats:italic>\n                    eradication. This study has confirmed the presence of genes previously implicated in the pathogenesis of\n                    <jats:italic>H pylori<\/jats:italic>\n                    , as well as highlighted novel candidates for further investigation.\n                  <\/jats:p>","DOI":"10.1136\/gut.2006.095646","type":"journal-article","created":{"date-parts":[[2006,4,26]],"date-time":"2006-04-26T20:29:08Z","timestamp":1146083348000},"page":"1717-1724","source":"Crossref","is-referenced-by-count":61,"title":["Global analysis of the human gastric epithelial transcriptome altered by\n                    <i>Helicobacter pylori<\/i>\n                    eradication in vivo"],"prefix":"10.1136","volume":"55","author":[{"given":"M B","family":"Resnick","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Sabo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P A","family":"Meitner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S S","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y","family":"Cho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H K","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Tavares","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S F","family":"Moss","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[2006,4,26]]},"reference":[{"key":"2025120519045203000_55.12.1717.1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra020542"},{"key":"2025120519045203000_55.12.1717.2","doi-asserted-by":"publisher","DOI":"10.1007\/s00281-005-0204-8"},{"key":"2025120519045203000_55.12.1717.3","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200401240"},{"key":"2025120519045203000_55.12.1717.4","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.20047"},{"key":"2025120519045203000_55.12.1717.5","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.182558799"},{"key":"2025120519045203000_55.12.1717.6","first-page":"145","volume":"16","year":"2002","journal-title":"Aliment Pharmacol Ther"},{"key":"2025120519045203000_55.12.1717.7","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.2.988-992.2002"},{"key":"2025120519045203000_55.12.1717.8","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.11.6970-6980.2001"},{"key":"2025120519045203000_55.12.1717.9","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2001.5006"},{"key":"2025120519045203000_55.12.1717.10","doi-asserted-by":"publisher","DOI":"10.1136\/gut.48.5.598"},{"key":"2025120519045203000_55.12.1717.11","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200111374"},{"key":"2025120519045203000_55.12.1717.12","doi-asserted-by":"publisher","DOI":"10.1080\/00365520410003588"},{"key":"2025120519045203000_55.12.1717.13","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.4.2595"},{"key":"2025120519045203000_55.12.1717.14","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.9.5216-5226.2004"},{"key":"2025120519045203000_55.12.1717.15","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2004.08.054"},{"key":"2025120519045203000_55.12.1717.16","doi-asserted-by":"publisher","DOI":"10.1136\/mp.56.1.36"},{"key":"2025120519045203000_55.12.1717.17","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/18.11.1540"},{"key":"2025120519045203000_55.12.1717.18","doi-asserted-by":"crossref","unstructured":"Storey JD, Tibshirani R. 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