{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T04:39:30Z","timestamp":1791175170804,"version":"4.1.0"},"reference-count":39,"publisher":"BMJ","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"published-print":{"date-parts":[[2009,5]]},"abstract":"\n Background and aims:<\/jats:title>\n \n Helicobacter pullorum<\/jats:italic>\n is an enterohepatic\n Helicobacter<\/jats:italic>\n species of avian origin detected in patients with acute diarrhoea and inflammatory bowel disease. The aim of the present study was to determine whether\n H pullorum<\/jats:italic>\n exerts a direct effect on human intestinal epithelial cells in vitro and to characterise the bacterial mechanisms and the signalling pathways involved.\n <\/jats:p>\n <\/jats:sec>\n \n Materials and methods:<\/jats:title>\n \n The proinflammatory properties of\n H pullorum<\/jats:italic>\n from human and avian origins were measured on human gastric (AGS) and intestinal (CaCo-2 and HT-29) epithelial cell lines after co-culture with different\n H pullorum<\/jats:italic>\n strains, and the extent of nuclear factor-\u03baB (NF-\u03baB) involvement was determined.\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n All of the\n H pullorum<\/jats:italic>\n strains tested stimulated interleukin 8 (IL8) secretion by the three cell lines. Similar results were obtained with heat-killed\n H pullorum<\/jats:italic>\n . Incubation of cells with filtered\n H pullorum<\/jats:italic>\n culture supernatants did not stimulate IL8 secretion. The same observation was made when bacterial adherence was inhibited by Transwell inserts.\n H pullorum<\/jats:italic>\n induced NF-\u03baB activation and rapid nuclear translocation as demonstrated by immunofluorescent staining and cellular fractionation. NF-\u03baB involvement was confirmed by using the specific inhibitor SN50 and small interfering RNA (siRNA) which abolished\n H pullorum<\/jats:italic>\n -induced IL8 production.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions:<\/jats:title>\n \n H pullorum<\/jats:italic>\n strains stimulate IL8 secretion by human gastric and intestinal epithelial cell lines. This effect requires bacterial adherence and probably lipopolysaccharides, and is mediated by NF-\u03baB signalling. The present study strengthens the argument that\n H pullorum<\/jats:italic>\n is a potent human pathogen and highlights its putative role in acute and chronic digestive diseases such as inflammatory bowel disease.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1136\/gut.2007.144501","type":"journal-article","created":{"date-parts":[[2008,6,25]],"date-time":"2008-06-25T21:06:44Z","timestamp":1214428004000},"page":"629-635","source":"Crossref","is-referenced-by-count":37,"title":["Study of\n Helicobacter pullorum<\/i>\n proinflammatory properties on human epithelial cells in 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