{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T17:15:18Z","timestamp":1765646118826,"version":"3.48.0"},"reference-count":17,"publisher":"BMJ","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"published-print":{"date-parts":[[1980,5]]},"abstract":"<jats:p>Trypsin in duodenal aspirate and pure pancreatic juice samples has been measured by both radioimmunoassay and enzymatic mathods. The radioimmunossay has been shown to be specific and to detect trypsin in the presence of aprotinin (Trasylol). In duodenal juice samples from control subjects and from patients with primary biliary cirrhosis a good correlation was obtained between both immunoreactive trypsin concentration and trypsin activity. The immunoreactive trypsinogen concentration in pure pancreatic juice also correlated well with activated trypsin activity. The mean immunoreactive trypsin concentration and the concentration of enzymatically inert immunoreactive trypsin in duodenal juice samples from patients with primary biliary cirrhosis were significantly lower than controls, suggesting pancreatic hyposecretion in this disease. After five duodenal juice samples had been stored for three months at -70 degrees C, immunoreactive trypsin concentration in samples stored without Trasylol were reduced by 12 . 0 +\/- 4 . 2 (mean +\/- SD) times the concentration of samples stored in Trasylol. Trypsin autodegradation continues even at -70 degrees C; Trasylol protects against this. Radioimmunoassay is a reliable method of trypsin estimation in duodenal juice and has advantages over conventional enzymatic analysis.<\/jats:p>","DOI":"10.1136\/gut.21.5.402","type":"journal-article","created":{"date-parts":[[2007,9,18]],"date-time":"2007-09-18T17:41:31Z","timestamp":1190137291000},"page":"402-407","source":"Crossref","is-referenced-by-count":16,"title":["Measurement of trypsin in duodenal juice by radioimmunoassay."],"prefix":"10.1136","volume":"21","author":[{"given":"G","family":"Lake-Bakaar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"McKavanagh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C E","family":"Rubio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O","family":"Epstein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J A","family":"Summerfield","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1980,5,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/S0140-6736(77)90066-6","article-title":"Diagnostic importance of changes in circulating concentrations of immunoreactive trypsin","volume":"2","author":"Elias, E.; Redshaw, M.; Wood, T.","year":"1977","unstructured":"Elias E, Redshaw M, Wood T. Diagnostic importance of changes in circulating concentrations of immunoreactive trypsin. Lancet 1977; 2: 66-8.","journal-title":"Lancet"},{"key":"ref_2","first-page":"234","article-title":"Die Bedeutung der Aktivierung und Inaktivierung von proteolytischen Enzymen fur die Funktionsdiagnostik des Pankreas","volume":"5","author":"Miller-Wieland, K.; Berndt, W.","year":"1967","unstructured":"-2Miller-Wieland K, Berndt W. Die Bedeutung der Aktivierung und Inaktivierung von proteolytischen Enzymen fur die Funktionsdiagnostik des Pankreas. Z Gastroenterol 1967; 5: 234-8.","journal-title":"Z Gastroenterol"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1016\/S0016-5085(74)80043-0","article-title":"Role of bile acids in the activation of trypsinogen by enterokinase: disturbance of trypsinogen activation in patients with intrahepatic biliary atresia","volume":"66","author":"Hadorn, B.; Hess, J.; Troesch, V.; Verhaage, W.; Gotze, H.; Bender, S.W.","year":"1974","unstructured":"Hadorn B, Hess J, Troesch V, Verhaage W, Gotze H, Bender SW. Role of bile acids in the activation of trypsinogen by enterokinase: disturbance of trypsinogen activation in patients with intrahepatic biliary atresia. Gastroenterology 1974; 66: 548-55.","journal-title":"Gastroenterology"},{"key":"ref_4","first-page":"200","article-title":"Effect of bile acids and ionic strength on trypsinogen activation by human enteropeptidase","volume":"525","author":"Rinderknecht, H.; Friedman, R.","year":"1978","unstructured":"Rinderknecht H, Friedman R. Effect of bile acids and ionic strength on trypsinogen activation by human enteropeptidase. Biochem Biophys Acta 1978; 525: 200-8.","journal-title":"Biochem Biophys Acta"},{"key":"ref_5","volume-title":"Methods of enzymatic analysis","volume":"2","author":"HU, Rick W.In Bergmeyer","unstructured":"Rick W. In Bergmeyer HU, ed. Methods of enzymatic analysis, vol. 2, 2nd English edition, Weinheim\/New York and London: Verlag Chemie\/Academic Press, 1974: 1013."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1393","DOI":"10.1139\/y59-157","article-title":"A modified spectrophotometric determination of chymotrypsin, trypsin, and thrombin","volume":"37","author":"Hummel, B.C.W.","year":"1959","unstructured":"Hummel BCW. A modified spectrophotometric determination of chymotrypsin, trypsin, and thrombin. Canad J Biochem Physiol 1959; 37: 1393-9.","journal-title":"Canad J Biochem Physiol"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1002\/bjs.1800520615","article-title":"The effect of bile on the activation of trypsinogen and the activity of trypsin in pancreatic juice","volume":"52","author":"Elmslie, R.","year":"1965","unstructured":"Elmslie R. The effect of bile on the activation of trypsinogen and the activity of trypsin in pancreatic juice. Br J Surg 1965; 52: 465-70. 8Lowry OH; Rosebrough NJ, Farr AL, Randall RJ.","journal-title":"Br J Surg"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the Folin phenol reagent","volume":"193","year":"1951","unstructured":"Protein measurement with the Folin phenol reagent. J Biol Chem 1951; 193: 265-75.","journal-title":"J Biol Chem"},{"key":"ref_9","first-page":"78","article-title":"Radioimmunoassay of enzymes of the exocrine pancreas (trypsin, chymotrypsin, chymotrypsinogen)","volume":"236","author":"Temler, R.S.; Felber, J.P.","year":"1971","unstructured":"Temler RS, Felber JP. Radioimmunoassay of enzymes of the exocrine pancreas (trypsin, chymotrypsin, chymotrypsinogen). Biochem Biophys Acta 1971; 236: 78-83.","journal-title":"Biochem Biophys Acta"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2943","DOI":"10.1016\/S0021-9258(18)93363-6","article-title":"Chromatography of trypsin and its derivatives: characterization of a new active form of bovine trypsin","volume":"243","author":"Schroeder, D.D.; Shaw, E.","year":"1968","unstructured":"Schroeder DD, Shaw E. Chromatography of trypsin and its derivatives: characterization of a new active form of bovine trypsin. J Biol Chem 1968; 243: 2943-9.","journal-title":"J Biol Chem"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"5610","DOI":"10.1016\/S0021-9258(18)96388-X","article-title":"Trypsin inhibitor from bovine pancreatic juice","volume":"241","author":"Greene, L.J.; Rigbi, M.; Fackre, D.S.","year":"1966","unstructured":"Greene LJ, Rigbi M, Fackre DS. Trypsin inhibitor from bovine pancreatic juice. J Biol Chem 1966; 241: 5610-8.","journal-title":"J Biol Chem"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1016\/S0140-6736(70)90167-4","article-title":"Stimulation of pancreatic secretion by intraduodenal infusion of bile-salts","volume":"2","author":"Wormsley, K.G.","year":"1970","unstructured":"Wormsley KG. Stimulation of pancreatic secretion by intraduodenal infusion of bile-salts. Lancet 1970; 2: 586-8.","journal-title":"Lancet"},{"key":"ref_13","first-page":"180","article-title":"Exocrine pancreatic secretion and immunoreactive secretion (IRS) release after intraduodenal instillation of bile in man","volume-title":"Gut","volume":"19","year":"1978","unstructured":"Osnes M, Hanssen LE, Flaten 0, Myren J. Exocrine pancreatic secretion and immunoreactive secretion (IRS) release after intraduodenal instillation of bile in man. Gut 1978; 19: 180-4."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"261","DOI":"10.3109\/00365527109180705","article-title":"The influence of bile and pure bile salts on pancreatic secretion in man","volume":"6","author":"Forell, M.M.; Otte, M.; Kohl, H.J.; Lehnert, P.; Stahiheber, H.P.","year":"1971","unstructured":"Forell MM, Otte M, Kohl HJ, Lehnert P, Stahiheber HP. The influence of bile and pure bile salts on pancreatic secretion in man. Scand J Gastrol 1971; 6: 261-5.","journal-title":"Scand J Gastrol"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/S0022-3476(65)80184-6","article-title":"Trypsinogen deficiency disease","volume":"66","author":"Townes, P.L.","year":"1965","unstructured":"Townes PL. Trypsinogen deficiency disease. J Paedr 1965; 66: 275-85.","journal-title":"J Paedr"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1016\/S0022-3476(67)80076-3","article-title":"Further observations on trypsinogen deficiency disease: report of a second case","volume":"71","author":"Townes, P.L.; Bryson, M.F.; Miller, G.","year":"1967","unstructured":"Townes PL, Bryson MF, Miller G. Further observations on trypsinogen deficiency disease: report of a second case. J Paedr 1967; 71: 220-4.","journal-title":"J Paedr"},{"key":"ref_17","first-page":"203","article-title":"Trypsinogen deficiency disease","volume":"114","author":"Morris, M.D.; Fisher, D.A.","year":"1967","unstructured":"Morris MD, Fisher DA. Trypsinogen deficiency disease. Am J Dis Child 1967; 114: 203-8.","journal-title":"Am J Dis Child"}],"container-title":["Gut"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/gut.21.5.402","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T17:11:52Z","timestamp":1765645912000},"score":1,"resource":{"primary":{"URL":"https:\/\/gut.bmj.com\/lookup\/doi\/10.1136\/gut.21.5.402"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1980,5]]},"references-count":17,"journal-issue":{"issue":"5","published-online":{"date-parts":[[1980,5,1]]},"published-print":{"date-parts":[[1980,5]]}},"alternative-id":["10.1136\/gut.21.5.402"],"URL":"https:\/\/doi.org\/10.1136\/gut.21.5.402","relation":{},"ISSN":["0017-5749","1468-3288"],"issn-type":[{"type":"print","value":"0017-5749"},{"type":"electronic","value":"1468-3288"}],"subject":[],"published":{"date-parts":[[1980,5]]}}}