{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T21:34:33Z","timestamp":1765661673510,"version":"3.48.0"},"reference-count":8,"publisher":"BMJ","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"published-print":{"date-parts":[[1981,8]]},"abstract":"<jats:p>Jejunal biopsies from patients with coeliac disease and from controls were cultured in vitro for 24 hours with 14C-labelled leucine. The net rate of protein synthesis was found to be linear over 24 hours for mucosa from control subjects and patients with coeliac disease. Protein synthesis by mucosa from untreated coeliac patients was significantly greater than by control mucosa. Protein synthesis by treated gluten-sensitive coeliac mucosa was significantly less than that by untreated coeliac mucosa and did not differ from control mucosa. Protein synthesis by treated non-responsive coeliac mucosa was significantly less than untreated coeliac mucosa but greater than control mucosa. The differences in protein synthesis could not be accounted for by differences in the size of the enterocyte leucine pool. Analytical subcellular fractionation of cultured jejunal mucosa showed that most of the protein synthesised in vitro was found in the cytosol and endoplasmic reticulum-brush border fractions of the enterocyte.<\/jats:p>","DOI":"10.1136\/gut.22.8.623","type":"journal-article","created":{"date-parts":[[2007,9,18]],"date-time":"2007-09-18T17:41:31Z","timestamp":1190137291000},"page":"623-627","source":"Crossref","is-referenced-by-count":6,"title":["Protein synthesis by cultured jejunal mucosa from control subjects and patients with coeliac disease."],"prefix":"10.1136","volume":"22","author":[{"given":"P E","family":"Jones","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C L","family":"L'Hirondel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T J","family":"Peters","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1981,8,1]]},"reference":[{"issue":"725-31","key":"ref_1","first-page":"\"2L'Hirondel\" # \"425","article-title":"The enterocyte in coeliac disease","volume":"2","author":"Booth, C.C.","year":"1970","unstructured":"Booth CC. The enterocyte in coeliac disease. Br Med J 1970; 2:725-31. 2L'Hirondel C, Doe WF, Peters TJ. Biochemical and morphological studies on human jejunal mucosa maintained in culture. Clin Sci Mol Med 1976; 50: 425-8.","journal-title":"Br Med J; Biochemical and morphological studies on human jejunal mucosa maintained in culture"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1130","DOI":"10.1136\/bmj.1.6069.1130","article-title":"DNA synthesis by jejunal mucosa in responsive and non-responsive coeliac disease","volume":"1","author":"Jones, P.E.; Peters, T.J.","year":"1977","unstructured":"Jones PE, Peters TJ. DNA synthesis by jejunal mucosa in responsive and non-responsive coeliac disease. Br Med J 1977; 1:1130-1.","journal-title":"Br Med J"},{"key":"ref_3","first-page":"293","article-title":"Analytical subcellular fractionation of jejunal biopsy specimens: enzyme activities, organelle pathology and response to corticosteroids in patients with nonresponsive coeliac disease","volume":"55","author":"Peters, T.J.; Jones, P.E.; Jenkins, W.J.; Wells, G.","year":"1978","unstructured":"Peters TJ, Jones PE, Jenkins WJ, Wells G. Analytical subcellular fractionation of jejunal biopsy specimens: enzyme activities, organelle pathology and response to corticosteroids in patients with nonresponsive coeliac disease. Clin Sci Mol Med 1978; 55:293-300.","journal-title":"Clin Sci Mol Med"},{"key":"ref_4","first-page":"100","article-title":"Oral zinc supplements in nonresponsive coeliac disease. Effect on jejunal morphology, enterocyte production and brush border disaccharidase activities. Gut 1981; 22: 194-8. '! Le Pecq JB, Paoletti C. A new fluorimetric method for RNA and DNA determination","volume":"17","author":"Jones, P.E.; Peters, T.J.","year":"1966","unstructured":"Jones PE, Peters TJ. Oral zinc supplements in nonresponsive coeliac disease. Effect on jejunal morphology, enterocyte production and brush border disaccharidase activities. Gut 1981; 22:194-8. '!Le Pecq JB, Paoletti C. A new fluorimetric method for RNA and DNA determination. Anal Biochem 1966; 17:100-5.","journal-title":"Anal Biochem"},{"key":"ref_5","first-page":"557","article-title":"Analytical subcellular fractionation of jejunal biopsy specimens: methodology and characterisation of organelles in normal tissue","volume":"51","author":"Peters, T.J.","year":"1976","unstructured":"Peters TJ. Analytical subcellular fractionation of jejunal biopsy specimens: methodology and characterisation of organelles in normal tissue. Clin Sci Mol Med 1976; 51:557-74.","journal-title":"Clin Sci Mol Med"},{"key":"ref_6","first-page":"307","article-title":"Organ culture of human duodenum and jejunum","volume":"8","author":"xHauri HP; M, Kedinger; K, Haffen; JF, Grenier; B, Hadorn","year":"1975","unstructured":"xHauri HP, Kedinger M, Haffen K, Grenier JF, Hadorn B. Organ culture of human duodenum and jejunum. Biol Gastroenterol (Paris) 1975; 8:307-19.","journal-title":"Biol Gastroenterol (Paris)"},{"article-title":"Alkaline phosphatase synthesis and properties of subcellular organelles during in vitro culture of jejunal biopsies from control subjeots and patients with coeliac disease. Gut 1981; in press","author":"Jones, P.E.; Peters, L.'Hirondel C.; T.J.","key":"ref_7","unstructured":"Jones PE, L'Hirondel C. Peters TJ. Alkaline phosphatase synthesis and properties of subcellular organelles during in vitro culture of jejunal biopsies from control subjeots and patients with coeliac disease. Gut 1981; in press."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1172\/JCI106525","article-title":"Laster L Incorporation of L leucine-1'C into immunoglobulins by jejunal biopsies of patients with coeliac sprue and other gastrointestinal diseases","volume":"50","author":"Loeb, P.M.; Strober, W.; Falchuk, Z.M.","year":"1971","unstructured":"Loeb PM, Strober W, Falchuk ZM, Laster L Incorporation of L leucine-1'C into immunoglobulins by jejunal biopsies of patients with coeliac sprue and other gastrointestinal diseases. J Clin Invest 1971; 50:559-69.","journal-title":"J Clin Invest"}],"container-title":["Gut"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/gut.22.8.623","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T21:30:24Z","timestamp":1765661424000},"score":1,"resource":{"primary":{"URL":"https:\/\/gut.bmj.com\/lookup\/doi\/10.1136\/gut.22.8.623"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1981,8]]},"references-count":8,"journal-issue":{"issue":"8","published-online":{"date-parts":[[1981,8,1]]},"published-print":{"date-parts":[[1981,8]]}},"alternative-id":["10.1136\/gut.22.8.623"],"URL":"https:\/\/doi.org\/10.1136\/gut.22.8.623","relation":{},"ISSN":["0017-5749","1468-3288"],"issn-type":[{"type":"print","value":"0017-5749"},{"type":"electronic","value":"1468-3288"}],"subject":[],"published":{"date-parts":[[1981,8]]}}}