{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T14:46:04Z","timestamp":1765637164999,"version":"3.48.0"},"reference-count":15,"publisher":"BMJ","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"published-print":{"date-parts":[[1982,1]]},"abstract":"<jats:p>An improved technique for the detection of alpha-gliadin sensitised mononuclear cells in the peripheral blood of untreated coeliac patients is described. This method is a modification of the direct LMIF assay, and involves exposure of lymphocytes to alpha-gliadin and the assay of the resultant lymphokine produced using normal leucocytes as indicator cells. All untreated coeliac patients, 14 of 15 treated patients, and two of 28 controls responded to alpha-gliadin. The direct LMIF assay in comparison is less sensitive, and detected sensitivity to alpha-gliadin in only four out of eight patients with untreated coeliac disease. Use of the indirect LMIF technique demonstrates that in untreated as well as treated coeliac patients there are cells sensitised to alpha-gliadin circulating in the peripheral blood. These findings may have pathogenic and diagnostic significance.<\/jats:p>","DOI":"10.1136\/gut.23.1.83","type":"journal-article","created":{"date-parts":[[2007,9,18]],"date-time":"2007-09-18T17:41:31Z","timestamp":1190137291000},"page":"83-87","source":"Crossref","is-referenced-by-count":16,"title":["Cellular response to alpha-gliadin in untreated coeliac disease."],"prefix":"10.1136","volume":"23","author":[{"given":"C","family":"O'Farrelly","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Feighery","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J F","family":"Greally","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D G","family":"Weir","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1982,1,1]]},"reference":[{"issue":"2","key":"ref_1","first-page":"\"159","article-title":"Immune response to gluten in adult coeliac disease","volume":"1969","author":"Housley, J.; Asquith, P.; Cook, W.I.","year":"1966","unstructured":"Housley J, Asquith P, Cook WI. Immune response to gluten in adult coeliac disease. Br Med J 1969; 2:159-61. 2Hourihane DO'B. The pathology of malabsorption states. In: Harrison CV, ed. Recent advances in pathology London: Churchill, 1966: 320-47.","journal-title":"Br Med J; Recent advances in pathology; 2Hourihane DO'B. The pathology of malabsorption states"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1202","DOI":"10.1016\/S0140-6736(72)90924-5","article-title":"Antigen-antibody reactions in jejunal mucosa in childhood coeliac disease after gluten challenge","volume":"1","author":"Shiner, M.; Ballard, J.","year":"1972","unstructured":"Shiner M, Ballard J. Antigen-antibody reactions in jejunal mucosa in childhood coeliac disease after gluten challenge. 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Acta Med Scand 1967; 181:247-56.","journal-title":"Acta Med Scand"},{"issue":"224","key":"ref_7","first-page":"38","article-title":"Non antibody mediators of cellular immunity generated by lymphocyte activation","volume":"1969","author":"Dumonde, D.C.; Wolstencroft, R.A.; Panayi, G.S.; Matthew, M.; Morley, J.; Howson, W.I.","unstructured":"Dumonde DC, Wolstencroft RA, Panayi GS, Matthew M, Morley J, Howson WI. Non antibody mediators of cellular immunity generated by lymphocyte activation. Nature (Lond) 1969; 224:38-42.","journal-title":"Nature (Lond)"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1111\/j.1365-2222.1971.tb02453.x","article-title":"The clinical significance of mediators of cellular immunity","volume":"1","author":"Dumonde, D.C.; Maini, R.N.","year":"1971","unstructured":"Dumonde DC, Maini RN. The clinical significance of mediators of cellular immunity. 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Lancet 1978; 1:876.","journal-title":"Lancet"},{"key":"ref_11","first-page":"546","article-title":"Migration inhibitory effect of cell-free supernatants from tuberculin stimulated cultures of human mononuclear leukocytes demonstrated by two-step MIF agarose assay","volume":"110","author":"Clausen, J.E.","year":"1973","unstructured":"Clausen JE. Migration inhibitory effect of cell-free supernatants from tuberculin stimulated cultures of human mononuclear leukocytes demonstrated by two-step MIF agarose assay. JImmunol 1973; 110:546-51.","journal-title":"JImmunol"},{"key":"ref_12","first-page":"7","article-title":"Hekkens W Th JM, Haex AJ, Willihagen RGJ. Some aspects of gliadin fractionation and testing by a histochemical method","author":"Booth, C.C.","year":"1970","unstructured":"Hekkens W Th JM, Haex AJ, Willihagen RGJ. Some aspects of gliadin fractionation and testing by a histochemical method. In: Booth CC, Dowling RH, eds. 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