{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T16:41:10Z","timestamp":1763743270187,"version":"3.45.0"},"reference-count":13,"publisher":"BMJ","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"published-print":{"date-parts":[[1982,2]]},"abstract":"<jats:p>Portions of jejunal biopsies from control subjects and from patients with coeliac disease were cultured for 24 hours using an in vitro organ culture technique. Alkaline phosphatase activity was measured in the tissue and medium before and after culture; enzyme activities were expressed per microgram tissue DNA. The increase in enzyme activity during the culture period was taken to represent net enzyme synthesis. Alkaline phosphatase synthesis by mucosa from patients with untreated gluten-sensitive coeliac disease and by mucosa from patients with non-responsive coeliac disease was significantly less than that by normal mucosa. Alkaline phosphatase synthesis by mucosa from patients with treated gluten-sensitive coeliac disease was greater than that by untreated coeliac mucosa but was less than normal mucosa. Sequential studies of alkaline phosphatase synthesis by jejunal mucosa from seven patients with coeliac disease, before and after successful treatment by gluten withdrawal, showed an increase in synthesis in all patients. Study, by analytical subcellular fractionation with sucrose density gradient centrifugation, of the properties of the organelles of cultured control tissue showed good preservation of their integrity. A striking finding, however, was the decrease in malate dehydrogenase with a corresponding marked increase in lactate dehydrogenase. This would be expected to be followed by a shift from aerobic to anaerobic metabolism. Analytical subcellular fractionation of cultured mucosa from patients with coeliac disease gave similar conclusions. There was, however, a marked improvement of the brush border abnormalities, characteristic of coeliac disease, during culture with increased enzyme activities and membrane equilibrium density in the sucrose gradients.<\/jats:p>","DOI":"10.1136\/gut.23.2.108","type":"journal-article","created":{"date-parts":[[2007,9,7]],"date-time":"2007-09-07T21:40:01Z","timestamp":1189201201000},"page":"108-114","source":"Crossref","is-referenced-by-count":6,"title":["Alkaline phosphatase synthesis and properties of subcellular organelles during in vitro culture of jejunal biopsies from control subjects and patients with coeliac disease."],"prefix":"10.1136","volume":"23","author":[{"given":"P E","family":"Jones","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"L'Hirondel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T J","family":"Peters","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1982,2,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1136\/gut.9.1.96","article-title":"Alkaline phosphatase levels in normal and diseased small bowel","volume":"9","author":"Ferguson, A.; Watson, W.C.; Maxwell, J.D.; Fell, G.S.","year":"1968","unstructured":"Ferguson A, Watson WC, Maxwell JD, Fell GS. Alkaline phosphatase levels in normal and diseased small bowel. Gut 1968; 9:96-8.","journal-title":"Gut"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1111\/j.1445-5994.1972.tb03066.x","article-title":"Mucosal enzyme activity as a quantitative index of early functional improvement in the management of coeliac disease and other small intestinal disease","volume":"2","author":"Campbell, C.B.; Cowen, A.E.; McGeary, H.M.; Gaffney, T.J.","year":"1972","unstructured":"Campbell CB, Cowen AE, McGeary HM, Gaffney TJ. Mucosal enzyme activity as a quantitative index of early functional improvement in the management of coeliac disease and other small intestinal disease. Aust NZ J Med 1972; 2:220-27.","journal-title":"Aust NZ J Med"},{"key":"ref_3","first-page":"259","article-title":"Enzyme activities and properties of lysosomes and brush borders in jejunal biopsies from control subjects and patients with coeliac disease","volume":"48","author":"Peters, T.J.; Heath, JR, Wansbrough-Jones; MH, Doe; W.F.","year":"1975","unstructured":"Peters TJ, Heath JR, Wansbrough-Jones MH, Doe WF. Enzyme activities and properties of lysosomes and brush borders in jejunal biopsies from control subjects and patients with coeliac disease. Clin Sci Mol Med 1975; 48:259-67.","journal-title":"Clin Sci Mol Med"},{"key":"ref_4","first-page":"285","article-title":"Analytical subcellular fractionation ofjejunal biopsy specimens; enzyme activities, organelle pathology and response to gluten withdrawal in patients with coeliac disease","volume":"55","author":"Peters, T.J.; Jones, P.E.; Wells, G.","year":"1978","unstructured":"Peters TJ, Jones PE, Wells G. Analytical subcellular fractionation ofjejunal biopsy specimens; enzyme activities, organelle pathology and response to gluten withdrawal in patients with coeliac disease. Clin Sci Mol Med 1978; 55:285-92.","journal-title":"Clin Sci Mol Med"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1172\/JCI107582","article-title":"An in vitro model of gluten sensitive enteropathy. Effect of gliadin on intestinal epithelial cells of patients with glutensensitive enteropathy in organ culture","volume":"53","author":"Falchuk, Z.M.; Gebhard, R.L.; Sessoms, C.; Strober, W.","year":"1974","unstructured":"Falchuk ZM, Gebhard RL, Sessoms C, Strober W. An in vitro model of gluten sensitive enteropathy. Effect of gliadin on intestinal epithelial cells of patients with glutensensitive enteropathy in organ culture. J Clin Invest 1974; 53:487-500.","journal-title":"J Clin Invest"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1080\/00365521.1975.12096935","article-title":"In vitro pathogenetic studies of coeliac disease","volume":"10","author":"Jos, J.; Lenoir, G.; De Ritis, G.; Rey, J.","year":"1975","unstructured":"Jos J, Lenoir G, De Ritis G, Rey J. In vitro pathogenetic studies of coeliac disease. Scand J Gastroenterol 1975; 10:121-8.","journal-title":"Scand J Gastroenterol"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1090","DOI":"10.1136\/gut.19.12.1090","article-title":"Re-evaluation of the technique of organ culture for studying gluten toxicity in coeliac disease","volume":"19","author":"Hauri, H.P.; Kedinger, M.; Haffen, K.; Gaze, H.; Hadorn, B.; Hekkens, W.","year":"1978","unstructured":"Hauri HP, Kedinger M, Haffen K, Gaze H, Hadorn B, Hekkens W. Re-evaluation of the technique of organ culture for studying gluten toxicity in coeliac disease. Gut 1978; 19:1090-8.","journal-title":"Gut"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1136\/gut.15.10.805","article-title":"Enzyme changes in human small bowel mucosa during culture in vitro","volume":"15","author":"Mitchell, J.D.; Mitchell, J.; Peters, T.J.","year":"1974","unstructured":"Mitchell JD, Mitchell J, Peters TJ. Enzyme changes in human small bowel mucosa during culture in vitro. Gut 1974; 15:805-11.","journal-title":"Gut"},{"key":"ref_9","first-page":"111","article-title":"Sequential biochemical studies in coeliac disease using jejunal organ culture techniques. In: Perspectives in Coeliac Disease. McNicholl BM","author":"Jones, P.E.; L'Hirondel, C.; Peters, T.J.","year":"1978","unstructured":"Jones PE, L'Hirondel C, Peters TJ. Sequential biochemical studies in coeliac disease using jejunal organ culture techniques. In: Perspectives in Coeliac Disease. McNicholl BM, McCarthy CF, Fottrell PF, eds. Lancaster: MTP Press. 1978: 111-8."},{"key":"ref_10","first-page":"\"425","article-title":"Biochemical and morphological studies on human jejunal mucosa maintained in culture","volume":"50","author":"L'Hirondel, C.; Doe, W.F.; Peters, T.J.","year":"1976","unstructured":"L'Hirondel C, Doe WF, Peters TJ. Biochemical and morphological studies on human jejunal mucosa maintained in culture. Clin Sci Mol Med 1976; 50:425-9. \"Le Pecq JB, Paoletti C. A new fluorimetric method for RNA and DNA determination. Analyt Biochem 1966; 17:100-7.","journal-title":"Clin Sci Mol Med; Le Pecq JB, Paoletti C. A new fluorimetric method for RNA and DNA determination"},{"key":"ref_11","first-page":"557","article-title":"The analytical subcellular fractionation of jejunal biopsy specimens. Methodology and characterisation of the organelles in normal tissue","volume":"51","author":"Peters, T.J.","year":"1976","unstructured":"Peters TJ. The analytical subcellular fractionation of jejunal biopsy specimens. Methodology and characterisation of the organelles in normal tissue. Clin Sci Mol Med 1976; 51:557-574.","journal-title":"Clin Sci Mol Med"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1007\/BF02939361","article-title":"Organ culture of coeliac and non-coeliac small intestinal mucosa","volume":"147","author":"Stevens, F.M.; Keane, R.; Fottrell, P.F.; McNicholls, B.; McCarthy, C.F.","year":"1978","unstructured":"Stevens FM, Keane R, Fottrell PF, McNicholls B, McCarthy CF. Organ culture of coeliac and non-coeliac small intestinal mucosa. Irish JMed Sci 1978; 147:11-4.","journal-title":"Irish JMed Sci"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1245","DOI":"10.1056\/NEJM197012032832302","article-title":"Epithelial cell renewal in cultured duodenal biopsies in coeliac sprue","volume":"283","author":"I5Trier, J.S.; Browning, T.H.","year":"1970","unstructured":"; 1:1130-1. I5Trier JS, Browning TH. Epithelial cell renewal in cultured duodenal biopsies in coeliac sprue. N Engl J Med 1970;283:1245-50.","journal-title":"N Engl J Med"}],"container-title":["Gut"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/gut.23.2.108","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T16:37:23Z","timestamp":1763743043000},"score":1,"resource":{"primary":{"URL":"https:\/\/gut.bmj.com\/lookup\/doi\/10.1136\/gut.23.2.108"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1982,2]]},"references-count":13,"journal-issue":{"issue":"2","published-online":{"date-parts":[[1982,2,1]]},"published-print":{"date-parts":[[1982,2]]}},"alternative-id":["10.1136\/gut.23.2.108"],"URL":"https:\/\/doi.org\/10.1136\/gut.23.2.108","relation":{},"ISSN":["0017-5749","1468-3288"],"issn-type":[{"type":"print","value":"0017-5749"},{"type":"electronic","value":"1468-3288"}],"subject":[],"published":{"date-parts":[[1982,2]]}}}