{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T08:08:33Z","timestamp":1765008513265,"version":"3.46.0"},"reference-count":24,"publisher":"BMJ","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"published-print":{"date-parts":[[1982,3]]},"abstract":"<jats:p>Using an indirect immunofluorescence technique liver membrane antibodies of IgG and IgA class have been demonstrated in a statistically significant proportion of sera from patients with alcoholic hepatitis and alcoholic cirrhosis. IgG and IgA class antibodies were found respectively in 23 and 25% of 48 patients with alcoholic hepatitis, in 27 and 33% of 84 with active cirrhosis, and 67 and 58% of 12 with inactive cirrhosis. These results provide evidence of a humoral immune response in alcoholic liver disease which is directed against, as yet undefined, liver-cell membrane antigens.<\/jats:p>","DOI":"10.1136\/gut.23.3.221","type":"journal-article","created":{"date-parts":[[2007,9,18]],"date-time":"2007-09-18T17:41:31Z","timestamp":1190137291000},"page":"221-225","source":"Crossref","is-referenced-by-count":41,"title":["Liver membrane antibodies in alcoholic liver disease: 1. prevalence and immunoglobulin class."],"prefix":"10.1136","volume":"23","author":[{"given":"A D","family":"Burt","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R S","family":"Anthony","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W S","family":"Hislop","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I A","family":"Bouchier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R N","family":"MacSween","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1982,3,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1016\/0016-5085(80)90157-2","article-title":"Alcohol, protein metabolism, and liver injury","volume":"79","author":"Lieber, C.S.","year":"1980","unstructured":"Lieber CS. 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Progress in liver diseases, vol. 5. New York: Grune and Stratton, 1976: 516-30."},{"key":"ref_4","first-page":"309","article-title":"Immune mechanisms in alcoholic liver disease","volume-title":"and the liver","author":"Fox, R.A.","year":"1977","unstructured":"Fox RA. Immune mechanisms in alcoholic liver disease. In: Fisher MM, Rankin JG, eds. Alcohol and the liver. New York: Plenum Press, 1977: 309-20."},{"key":"ref_5","first-page":"193","article-title":"Alcoholic liver disease","volume-title":"advances in histopathology","author":"MacSween, R.N.M.","year":"1978","unstructured":"MacSween RNM. Alcoholic liver disease. In: Anthony PP, Woolf N, eds. Recent advances in histopathology. Edinburgh: Churchill Livingstone, 1978: 193-212."},{"key":"ref_6","first-page":"\"578","article-title":"Detection of a liver-membrane autoantibody in HBs Ag-negative chronic active hepatitiis","volume":"294","author":"Hopf, U.; Meyer zum Buschenfelde, K.H.; Arnold, W.","year":"1976","unstructured":"Hopf U, Meyer zum Buschenfelde KH, Arnold W. Detection of a liver-membrane autoantibody in HBs Ag-negative chronic active hepatitiis. N Engi J Med 1976; 294:578-82. 7 Jeejeebhoy KN, Ho J, Greenberg GR, Phillips MJ, Bruce-Robertson A, Sodtke U. Albumin, fibrinogen and transferrin synthesis in isolated rat hepatocyte suspensions. A model for the study of plasma protein synthesis. Biochem J 1975; 146:141-55.","journal-title":"N Engi J Med; Albumin, fibrinogen and transferrin synthesis in isolated rat hepatocyte suspensions. A model for the study of plasma protein synthesis"},{"key":"ref_7","first-page":"7","article-title":"Ion exchange chromatography and gel filtration","volume-title":"Handbook ofexperimental immunology","volume":"1","author":"Fahay, J.L.; Terry, E.W.","year":"1973","unstructured":"Fahay JL, Terry EW. Ion exchange chromatography and gel filtration. In: Weir DM, ed. Handbook ofexperimental immunology, volume 1. Immunochemistry. Oxford: Blackwell, 1973: 7-1-7.16."},{"issue":"89","key":"ref_8","first-page":"230","article-title":"Separation of univalent fragments from the bivalent rabbit antibody molecule by reduction of disulphide bonds","volume":"1960","author":"Nisonoff, A.; Wissler, F.C.; Lipman, L.N.; Woernley, D.L.","unstructured":"Nisonoff A, Wissler FC, Lipman LN, Woernley DL. Separation of univalent fragments from the bivalent rabbit antibody molecule by reduction of disulphide bonds. Arch Biochem Biophys 1960; 89:230-44.","journal-title":"Arch Biochem Biophys"},{"key":"ref_9","first-page":"378","article-title":"Naturally occurring human antibodies to the F(ab')2 portion of IgG. Clin Exp Immunol","volume":"42","author":"Nasu, H.; Chia, D.S.; Knutson, D.W.; Barnett, E.V.","year":"1980","unstructured":"Nasu H, Chia DS, Knutson DW, Barnett EV. Naturally occurring human antibodies to the F(ab')2 portion of IgG. Clin Exp Immunol 1980; 42:378-86."},{"issue":"319-24","key":"ref_10","first-page":"12","article-title":"The mechanism of complement fixation by rabbit F(ab') 2: properties of the bactericidal reaction","volume":"14","author":"Steele, E.J.; Rowley, D.","year":"1977","unstructured":"Steele EJ, Rowley D. The mechanism of complement fixation by rabbit F(ab')2: properties of the bactericidal reaction. Immunochemistry 1977; 14:319-24. 12 McFarlane IG, Wojcicka BM, Zucker GM, Eddleston ALWF, Williams R. Purification and characterization of human liver-specific membrane lipoprotein (LSP). Clin Exp Immunol 1977; 27:381-90.","journal-title":"Immunochemistry; Purification and characterization of human liver-specific membrane lipoprotein (LSP). Clin Exp Immunol"},{"key":"ref_11","first-page":"1045","article-title":"Studies on 'liver specific' antigens. 1. Evaluation of the liver specificity of 'LSP'","volume-title":"and 'LP-2'. Gastroenterology 1979","volume":"77","author":"Behrens, U.J.; Paronetto, F.","unstructured":"Behrens UJ, Paronetto F. Studies on 'liver specific' antigens. 1. Evaluation of the liver specificity of 'LSP' and 'LP-2'. Gastroenterology 1979; 77:1045-52."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/0005-2736(73)90264-2","article-title":"A rapid method for the isolation of rat liver plasma membranes using an aqueous two-phase polymer system","volume":"311","author":"Lesko, L.; Donlon, M.; Marinetti, G.V.; Hare, J.D.","year":"1973","unstructured":"Lesko L, Donlon M, Marinetti GV, Hare JD. A rapid method for the isolation of rat liver plasma membranes using an aqueous two-phase polymer system. Biochim Biophys Acta 1973; 311:173-9.","journal-title":"Biochim Biophys Acta"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with Folin phenol reagent","volume":"193","author":"Lowry, O.H.; Rosebrough, M.J.; Farr, A.L.; Randall, R.I.","year":"1951","unstructured":"Lowry OH, Rosebrough MJ, Farr AL, Randall RI. Protein measurement with Folin phenol reagent. 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