{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T17:17:29Z","timestamp":1763745449629,"version":"3.45.0"},"reference-count":29,"publisher":"BMJ","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"published-print":{"date-parts":[[1983,4]]},"abstract":"<jats:p>Results of the direct leucocyte migration inhibition (LMI) test using gluten fraction III as antigen were unaffected by incorporation of puromycin into the culture medium at concentrations shown to prevent lymphokine mediated inhibition. Results of the LMI test performed with purified polymorphs were similar to and correlated with results of the standard LMI test using mixed leucocytes in both coeliacs and controls. The addition of purified T lymphocytes did not increase migration inhibition. Normal leucocytes incubated with serum from coeliac patients and washed showed marked migration inhibition when incubated with gluten fraction III. This sensitisation of normal leucocytes was prevented by preincubation with aggregated human IgG. These results suggest that leucocyte migration inhibition by gluten in coeliac disease is not due to lymphokine production by sensitised lymphocytes but is caused by cytophilic antibody.<\/jats:p>","DOI":"10.1136\/gut.24.4.311","type":"journal-article","created":{"date-parts":[[2007,9,18]],"date-time":"2007-09-18T17:41:31Z","timestamp":1190137291000},"page":"311-317","source":"Crossref","is-referenced-by-count":7,"title":["Leucocyte migration inhibition test in coeliac disease - a reappraisal."],"prefix":"10.1136","volume":"24","author":[{"given":"F G","family":"Simpson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H P","family":"Field","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P D","family":"Howdle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D A","family":"Robertson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M S","family":"Losowsky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1983,4,1]]},"reference":[{"key":"ref_1","first-page":"66","article-title":"Coeliac disease","volume-title":"Immunology of the gastrointestinal tract","author":"Asquith, P.; Haeney, M.R.","year":"1979","unstructured":"Asquith P, Haeney MR. Coeliac disease. In: Asquith P, ed. Immunology of the gastrointestinal tract. Edinburgh, London, and New York: Churchill Livingstone, 1979: 66-94."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1353","DOI":"10.1016\/S0140-6736(76)91996-6","article-title":"Gluten and lymphocyte sensitisation in coeliac disease","volume":"2","author":"Douwes, FR","year":"1976","unstructured":"Douwes FR. Gluten and lymphocyte sensitisation in coeliac disease. Lancet 1976; 2: 1353.","journal-title":"Lancet"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1136\/gut.19.2.126","article-title":"Cell mediated immunity to gluten fraction III in adult coeliac disease","volume":"19","author":"Bullen, A.W.; Losowsky, M.S.","year":"1978","unstructured":"Bullen AW, Losowsky MS. Cell mediated immunity to gluten fraction III in adult coeliac disease. 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