{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T13:21:07Z","timestamp":1793798467124,"version":"4.6.1"},"reference-count":30,"publisher":"BMJ","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"published-print":{"date-parts":[[1986,5]]},"abstract":"<jats:p>The relationship between lactose hydrolysis and absorption of released glucose was investigated by determining the kinetics of lactose digestion by jejunal biopsies incubated in vitro. Lactase activity in intact biopsies correlated with conventional assay of tissue homogenates (r = 0.85, p less than 0.001), and glucose uptake from 28 mM lactose was directly proportional to lactase activity (r = 0.95, p less than 0.001) in 21 subjects with normal lactase levels, six with hypolactasia (primary or secondary to coeliac disease) and two with lactose intolerance but normal lactase activity. Kinetic analysis at 0.56-56 mM lactose in five normal subjects showed saturable kinetics for hydrolysis (app Km = 33.9 +\/- 2.2 mM; app Vmax = 26.5 +\/- 1.1 nmol\/min\/mg dry weight) but glucose uptake could be fitted to a model either of saturable uptake (app Kt = 47.2 +\/- 0.3 mM; app Jmax = 14.1 +\/- 0.2 nmol\/min\/mg) or saturable uptake plus a linear component (app Kt = 21.3 +\/- 1.15; app Jmax = 4.59 +\/- 0.12; app Kd = 0.093 +\/- 0.010 nmol\/min\/mg\/mM). The proportion of glucose taken into the tissue did not significantly exceed 50% of the total released at any lactose concentration suggesting the lack of an efficient capture mechanism for the released glucose. The results suggest that lactose hydrolysis is the rate limiting step in the overall absorption of glucose from lactose in vitro, and that the relationship between hydrolysis and absorption is the same in normal subjects and in hypolactasic subjects.<\/jats:p>","DOI":"10.1136\/gut.27.5.521","type":"journal-article","created":{"date-parts":[[2007,9,18]],"date-time":"2007-09-18T17:41:31Z","timestamp":1190137291000},"page":"521-527","source":"Crossref","is-referenced-by-count":7,"title":["Lactose digestion by human jejunal biopsies: the relationship between hydrolysis and absorption."],"prefix":"10.1136","volume":"27","author":[{"given":"D J","family":"Dawson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R W","family":"Lobley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P C","family":"Burrows","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V","family":"Miller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Holmes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[1986,5,1]]},"reference":[{"key":"ref_1","first-page":"489","article-title":"Disaccharide absorption","volume-title":"normal and diseased human intestine. 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Gastroenterology 1966; 50: 340-6.","journal-title":"Gastroenterology"},{"key":"ref_4","first-page":"12","volume-title":"Intestinal lactase deficit in adults. Gastroenterology 1965","volume":"49","author":"Dunphy, J.V.; Littman, A.; Hammond, J.B.; Forstner, G.; Dahlqvist, A.; Crane, R.K.","unstructured":"Dunphy JV, Littman A, Hammond JB, Forstner G, Dahlqvist A, Crane RK. Intestinal lactase deficit in adults. Gastroenterology 1965; 49: 12-21."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1136\/gut.18.1.48","article-title":"Reinvestigation of lactose intolerant children: lack of correlation between continuing lactose intolerance and small intestinal morphology, disaccharidase activity and lactose tolerance tests","volume":"18","author":"Harrison, M.; Walker-Smith, J.A.","year":"1977","unstructured":"Harrison M, Walker-Smith JA. Reinvestigation of lactose intolerant children: lack of correlation between continuing lactose intolerance and small intestinal morphology, disaccharidase activity and lactose tolerance tests. Gut 1977; 18: 48-52.","journal-title":"Gut"},{"key":"ref_6","first-page":"831","article-title":"Editorial: lactose malabsorption and lactose intolerance","volume":"2","year":"1979","unstructured":"Editorial: lactose malabsorption and lactose intolerance. Lancet 1979; 2: 831-2.","journal-title":"Lancet"},{"key":"ref_7","first-page":"51","article-title":"Lactose malabsorption and gastrointestinal function: effects on gastrointestinal transit and the absorption of other nutrients. In: Paige DM, Bayless TM. Lactose digestion: clinical and nutritional implications","author":"Phillips, S.F.","year":"1981","unstructured":"Phillips SF. Lactose malabsorption and gastrointestinal function: effects on gastrointestinal transit and the absorption of other nutrients. In: Paige DM, Bayless TM. Lactose digestion: clinical and nutritional implications. Baltimore: John Hopkins University Press, 1981: 51-7."},{"key":"ref_8","first-page":"101","article-title":"Intestinal absorption by human jejunal biopsies: uptake of glucose","volume":"332","author":"Dawson, D.J.; Holmes, R.; Lobley, R.W.","year":"1982","unstructured":"Dawson DJ, Holmes R, Lobley RW. Intestinal absorption by human jejunal biopsies: uptake of glucose. J Physiol 1982; 332: 101-2P.","journal-title":"J Physiol"},{"key":"ref_9","first-page":"1198","article-title":"DGlucose: determination with hexokinase and glucose-6phosphate dehydrogenase","volume-title":"Methods of enzymatic analysis","author":"Bergmeyer, H.U.; Bernt, E.; Schmidt, F.; Stork, H.","year":"1974","unstructured":"Bergmeyer HU, Bernt E, Schmidt F, Stork H. DGlucose: determination with hexokinase and glucose-6phosphate dehydrogenase. In: Bergmeyer HU, ed. Methods of enzymatic analysis. 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Anal Biochem 1968; 22: 99-107.","journal-title":"Anal Biochem"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/S0003-9861(73)80023-2","article-title":"Fluorometric assay of proteins in the nanogram range","volume":"155","author":"Bohlen, P.; Stein, S.; Dairman, W.; Udenfriend, S.","year":"1973","unstructured":"Bohlen P, Stein S, Dairman W, Udenfriend S. Fluorometric assay of proteins in the nanogram range. Ann Biochem Biophys 1973; 155: 213-20.","journal-title":"Ann Biochem Biophys"},{"key":"ref_13","article-title":"Programs D506 and D516. CERN Computer 7600","author":"James, F.; Roos, M.","year":"1971","unstructured":"James F, Roos M. Programs D506 and D516. CERN Computer 7600. Geneva: Program Library, 1971."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1172\/JCI106268","article-title":"Renal and intestinal hexose transport in familial glucose-galactose malabsorption","volume":"49","author":"Elsas, L.J.; Hillman, R.E.; Patterson, J.H.; Rosenberg, L.E.","year":"1970","unstructured":"Elsas LJ, Hillman RE, Patterson JH, Rosenberg LE. Renal and intestinal hexose transport in familial glucose-galactose malabsorption. J Clin Invest 1970; 49: 576-85.","journal-title":"J Clin Invest"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1007\/BF01071905","article-title":"Sugar absorption by small bowel biopsy samples from patients with primary lactase deficiency and with adult coeliac disease","volume":"21","author":"Beck, I.T.; Dinda, P.K.; Da Costa, L.R.; Beck, M.","year":"1976","unstructured":"Beck IT, Dinda PK, Da Costa LR, Beck M. 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New York: Raven Press, 1981, 925-63."},{"key":"ref_21","first-page":"438","article-title":"Quantitative radioautography of sugar transport in intestinal biopsies from normal humans and a patient with glucose-galactose malabsorption","volume":"52","author":"Stirling, C.E.; Schneider, A.J.; Wong, M.-D.; Kinter, W.B.","year":"1972","unstructured":"Stirling CE, Schneider AJ, Wong M-D, Kinter WB. Quantitative radioautography of sugar transport in intestinal biopsies from normal humans and a patient with glucose-galactose malabsorption. J Clin Invest 1972; 52: 438-51.","journal-title":"J Clin Invest"},{"issue":"114","key":"ref_22","first-page":"349","article-title":"Human small intestinal P-galactosidases. Separation and characterisation of one lactase and one hetero-1-galactosidase","volume":"1969","unstructured":"Asp NG, Dahlqvist A, Koldovsky 0. Human small intestinal P-galactosidases. Separation and characterisation of one lactase and one hetero-1-galactosidase. 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Maltose hydrolysis and absorption in the human jejunum. Digestion 1982; 24: 137-45.","journal-title":"Digestion"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1007\/BF00582107","article-title":"Virtual elimination of the interference of unstirred water layers on intestinal sugar transport kinetics by use of the tissue accumulation method at appropriate shaking rates","volume":"389","author":"L'Herminier, M.; Alvarado, F.","year":"1981","unstructured":"L'Herminier M, Alvarado F. Virtual elimination of the interference of unstirred water layers on intestinal sugar transport kinetics by use of the tissue accumulation method at appropriate shaking rates. Pflugers Arch 1981 389: 155-8.","journal-title":"Pflugers Arch"},{"key":"ref_28","first-page":"71","article-title":"Monosaccharide absorption by human jejunal biopsies: anomalous behaviour of galactose","author":"Dawson, D.J.; Holmes, R.; Lobley, R.W.","year":"1983","unstructured":"Dawson DJ, Holmes R, Lobley RW. Monosaccharide absorption by human jejunal biopsies: anomalous behaviour of galactose. J Physiol 1983; 339: 54P. 29 Crane RK. Structural and functional organisation of an epithelial cell brush border. In: Warren KB, ed. Symposia of the International Society for Cell Biology. New York: Academic Press, 1966: 71-102.","journal-title":"J Physiol; Symposia of the International Society for Cell Biology; 339: 54P. 29 Crane RK. Structural and functional organisation of an epithelial cell brush border"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1113\/jphysiol.1971.sp009326","article-title":"Relationships between disaccharide hydrolysis and sugar transport in amphibian small intestine","volume":"212","author":"Parsons, D.S.; Prichard, J.S.","year":"1971","unstructured":"Parsons DS, Prichard JS. Relationships between disaccharide hydrolysis and sugar transport in amphibian small intestine. J Physiol 1971; 212: 299-319.","journal-title":"J Physiol"},{"issue":"5","key":"ref_30","first-page":"1","article-title":"Intestinal transfer mechanisms, measurements and analogies","volume":"24","author":"Smyth, D.H.","year":"1977","unstructured":"Smyth DH. Intestinal transfer mechanisms, measurements and analogies. J Clin Pathol 1977; 24 suppl 5: 1-9.","journal-title":"J Clin Pathol"}],"container-title":["Gut"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/gut.27.5.521","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T09:37:39Z","timestamp":1765618659000},"score":1,"resource":{"primary":{"URL":"https:\/\/gut.bmj.com\/lookup\/doi\/10.1136\/gut.27.5.521"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1986,5]]},"references-count":30,"journal-issue":{"issue":"5","published-online":{"date-parts":[[1986,5,1]]},"published-print":{"date-parts":[[1986,5]]}},"alternative-id":["10.1136\/gut.27.5.521"],"URL":"https:\/\/doi.org\/10.1136\/gut.27.5.521","relation":{},"ISSN":["0017-5749","1468-3288"],"issn-type":[{"value":"0017-5749","type":"print"},{"value":"1468-3288","type":"electronic"}],"subject":[],"published":{"date-parts":[[1986,5]]}}}