{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T03:32:10Z","timestamp":1772854330376,"version":"3.50.1"},"reference-count":28,"publisher":"BMJ","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"published-print":{"date-parts":[[1987,9]]},"abstract":"<jats:p>The uptake of ovalbumin (OA) from egg and beta-lactoglobulin (BLG) from cow's milk into the blood was investigated for seven hours after a test meal in five children with coeliac disease on a gluten free diet and after gluten challenge, and in five children with normal jejunal mucosa. Ovalbumin was detectable by ELISA in three of five coeliac children (maximal concentrations 8-178 ng\/ml serum) and in five of five controls (maximal 4-91 ng\/ml serum). Beta-lactoglobulin was detected in three of five coeliac children (maximal 0.6-6 ng\/ml serum) and in two of five controls (maximal 0.5 and 50 ng\/ml serum). No clear relationship was seen between maximal antigen concentrations and titres of serum IgG or IgA antibodies determined by ELISA, or as percentage antigen binding in a Farr type radioimmunoassay. Ovalbumin and beta-lactoglobulin was seen in serum of all coeliac patients and controls by HPLC fractionation in combination with ELISA, either in high MW fractions, or at the Mr of native OA and BLG, respectively. In one control degradation products (about 17 kD) of BLG were detectable in serum. The serum concentrations of OA and BLG were increased on gluten challenge in four or five coeliac children, indicating increased macromolecular passage through the gut mucosa in untreated coeliac disease.<\/jats:p>","DOI":"10.1136\/gut.28.9.1062","type":"journal-article","created":{"date-parts":[[2007,9,18]],"date-time":"2007-09-18T17:41:31Z","timestamp":1190137291000},"page":"1062-1072","source":"Crossref","is-referenced-by-count":66,"title":["Passage of dietary antigens into the blood of children with coeliac disease. Quantification and size distribution of absorbed antigens."],"prefix":"10.1136","volume":"28","author":[{"given":"S","family":"Husby","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N","family":"Foged","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"H\u00f8st","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S E","family":"Svehag","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1987,9,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1016\/0022-1759(80)90319-1","article-title":"Solid phase radioimmunoassay for detection of circulating food protein antigens in human serum","volume":"37","author":"R, I.Paganelli; RJ, Levinsky","year":"1980","unstructured":"I Paganelli R, Levinsky RJ. Solid phase radioimmunoassay for detection of circulating food protein antigens in human serum. J Immunol Meth 1980; 37: 333-41.","journal-title":"J Immunol Meth"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1159\/000233582","article-title":"The passage of maternal dietary protein into human breast milk","volume":"75","author":"Kilshaw, P.J.; Cant, A.J.","year":"1984","unstructured":"Kilshaw PJ, Cant AJ. The passage of maternal dietary protein into human breast milk. Int Arch Allergy Appl Imunol 1984; 75: 8-15.","journal-title":"Int Arch Allergy Appl Imunol"},{"issue":"22","key":"ref_3","first-page":"83","article-title":"Passage of undegraded dietary antigen into the blood of healthy adults. Quantification, estimation of size distribution and relation of uptake to levels of specific antibodies","volume":"1985","author":"Husby, S.; Jensenius, J.C.; Svehag, S.-E.","unstructured":"Husby S, Jensenius JC, Svehag S-E. Passage of undegraded dietary antigen into the blood of healthy adults. Quantification, estimation of size distribution and relation of uptake to levels of specific antibodies. Scand J Immunol 1985, 22: 83-92.","journal-title":"Scand J Immunol"},{"issue":"51","key":"ref_4","first-page":"662","article-title":"Immune reactions induced in infants by intestinal absorption of incompletely digested cow's milk protein","volume":"1936","author":"Lippard, W.W.; Schloss, O.M.; Johnson, P.A.","unstructured":"Lippard WW, Schloss OM, Johnson PA. Immune reactions induced in infants by intestinal absorption of incompletely digested cow's milk protein. Am J Dis Child 1936; 51: 662-74.","journal-title":"Am J Dis Child"},{"key":"ref_5","first-page":"763","article-title":"The gastrointestinal absorption of unaltered protein in normal infants and in infants recovering from diarrhoea","volume-title":"Pediatrics","volume":"16","author":"Gruskay, F.L.; Cooke, R.E.","year":"1955","unstructured":"Gruskay FL, Cooke RE. The gastrointestinal absorption of unaltered protein in normal infants and in infants recovering from diarrhoea. Pediatrics 1955; 16: 763-9."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1111\/j.1365-2222.1979.tb01552.x","article-title":"Intestinal uptake of ovalbumin in malabsorption and food allergy in relation to serum IgG antibody and orally administered sodium chromoglycate","volume":"9","author":"Dannaeus, A.; Inganas, M.; Johansson, S.G.O.; Foucard, T.","year":"1979","unstructured":"Dannaeus A, Inganas M, Johansson SGO, Foucard T. Intestinal uptake of ovalbumin in malabsorption and food allergy in relation to serum IgG antibody and orally administered sodium chromoglycate. Clin Allergy 1979, 9: 263-70.","journal-title":"Clin Allergy"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1136\/adc.58.3.201","article-title":"Differences between normal and milk allergic subjects in their immune response after milk ingestion","volume":"58","author":"Paganelli, R.; Atherton, D.J.; Levinsky, R.J.","year":"1983","unstructured":"Paganelli R, Atherton DJ, Levinsky RJ. Differences between normal and milk allergic subjects in their immune response after milk ingestion. Arch Dis Child 1983; 58: 201-6.","journal-title":"Arch Dis Child"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/0002-9343(84)90771-X","article-title":"Dietary protein antigenemia in humoral immunodeficiency. Correlation with splenomegaly","volume":"76","author":"Cunningham-Rundles, C.; Carr, R.J.; Good, R.A.","year":"1984","unstructured":"Cunningham-Rundles C, Carr RJ, Good RA. Dietary protein antigenemia in humoral immunodeficiency. Correlation with splenomegaly. Am J Med 1984; 76: 181-5.","journal-title":"Am J Med"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1172\/JCI109448","article-title":"Bovine antigens and the formation of circulating immune complexes in selective immunoglobulin A deficiency","volume":"69","author":"Cunningham-Rundles, C.; Brandeis, W.W.; Good, R.A.; Day, N.K.","year":"1979","unstructured":"Cunningham-Rundles C, Brandeis WW, Good RA, Day NK. Bovine antigens and the formation of circulating immune complexes in selective immunoglobulin A deficiency. J Clin Invest 1979; 69: 272-9.","journal-title":"J Clin Invest"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1136\/gut.11.8.635","article-title":"Immunoglobulins and dietary protein antibodies in childhood coeliac disease","volume":"11","author":"Kenrich, K.G.; Walker-Smith, J.A.","year":"1970","unstructured":"Kenrich KG, Walker-Smith JA. Immunoglobulins and dietary protein antibodies in childhood coeliac disease. Gut 1970; 11: 635-40.","journal-title":"Gut"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1136\/bmj.1.5792.75","article-title":"Precipitins to dietary proteins in serum and upper intestinal secretions of coeliac children","volume":"1","author":"Ferguson, A.; Carswell, F.","year":"1972","unstructured":"Ferguson A, Carswell F. Precipitins to dietary proteins in serum and upper intestinal secretions of coeliac children. Br MedJ 1972; 1: 75-7.","journal-title":"Br MedJ"},{"key":"ref_12","first-page":"25","article-title":"Immune response patterns in coeliac disease. Serum antibodies to dietary antigens measured by an enzyme linked immunosorbent assay (ELISA). Clin Exp Immunol","volume":"57","year":"1984","unstructured":"Scott H, Fausa 0, Ek J, Brandtzaeg P. Immune response patterns in coeliac disease. Serum antibodies to dietary antigens measured by an enzyme linked immunosorbent assay (ELISA). Clin Exp Immunol 1984; 57: 25-32."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1016\/S0140-6736(79)92507-8","article-title":"Abnormal intestinal permeability to sugars in villous atrophy","volume":"ii:","author":"Menzies, J.S.; Pounder, R.; Heyer, S.","year":"1979","unstructured":"Menzies JS, Pounder R, Heyer S, et al. Abnormal intestinal permeability to sugars in villous atrophy. Lancet 1979; ii: 1107-9.","journal-title":"Lancet"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/S0140-6736(83)91628-8","article-title":"A persistent defect in intestinal permeability in coeliac disease demonstrated by a 5'Cr-labelled EDTA absorption test","volume":"i:","author":"Bjarnason, J.; Peters, T.J.; Veall, N.","year":"1983","unstructured":"Bjarnason J, Peters TJ, Veall N. A persistent defect in intestinal permeability in coeliac disease demonstrated by a 5'Cr-labelled EDTA absorption test. Lancet 1983; i: 323-5.","journal-title":"Lancet"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/S0016-5085(19)32197-3","article-title":"Measurements of intestinal permeability using low molecular weight polyethylene glycols (PEG 400) II Application to normal and abnormal permeability states in man and animals","volume":"73","author":"Chadwick, V.S.; Phillips, S.F.; Hoffman, A.F.","year":"1977","unstructured":"Chadwick VS, Phillips SF, Hoffman AF. Measurements of intestinal permeability using low molecular weight polyethylene glycols (PEG 400) II Application to normal and abnormal permeability states in man and animals. Gastroenterology 1977; 73: 247-51.","journal-title":"Gastroenterology"},{"key":"ref_16","first-page":"801","article-title":"Small intestinal permeability to mannitol, lactulose and polyethylene glycol 400 in celiac disease","volume":"29","author":"Ukabam, S.O.; Cooper, B.T.","year":"1984","unstructured":"Ukabam SO, Cooper BT. Small intestinal permeability to mannitol, lactulose and polyethylene glycol 400 in celiac disease. Dig Dis Sci 1984; 29: 801-16.","journal-title":"Dig Dis Sci"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1136\/adc.57.6.462","article-title":"Intestinal absorption of food antigens in coeliac disease","volume":"57","author":"Pitcher-Wilmott, R.W.; Booth, I.; Harries, J.; Levinsky, R.J.","year":"1982","unstructured":"Pitcher-Wilmott RW, Booth I, Harries J, Levinsky RJ. Intestinal absorption of food antigens in coeliac disease. Arch Dis Child 1982; 57: 462-6.","journal-title":"Arch Dis Child"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1136\/adc.54.10.783","article-title":"The diagnosis of coeliac disease. A commentary on the current practices of members of the European Society for Paediatric Gastroenterology","volume":"54","author":"McNeish, A.S.; Harms, H.K.; Rey, J.","year":"1979","unstructured":"McNeish AS, Harms HK, Rey J, et al. The diagnosis of coeliac disease. A commentary on the current practices of members of the European Society for Paediatric Gastroenterology and Nutrition (ESPGAN) Arch Dis Child 1979; 54: 783-6.","journal-title":"and Nutrition (ESPGAN) Arch Dis Child"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/S0021-9673(01)89044-0","article-title":"Semiautomatic analysis of proteins and protein complexes by automated enzyme immunoassay after separation by high performance gel permeation chromatography","volume":"297","author":"Holmskov-Nielsen, U.; Erb, K.; Jensenius, J.C.","year":"1984","unstructured":"Holmskov-Nielsen U, Erb K, Jensenius JC. Semiautomatic analysis of proteins and protein complexes by automated enzyme immunoassay after separation by high performance gel permeation chromatography. J Chromatogr 1984; 297: 225-34.","journal-title":"J Chromatogr"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/0022-1759(83)90045-5","article-title":"Quantitation of human immunoglobulin by semiautomatic polyethylene glycol precipitation radioimmunoassays. Estimation of circulating immune complexes and immunoglobulin synthesized in vitro","volume":"56","author":"Jensenius, J.C.; Siersted, H.C.; Johnstone, A.P.","year":"1983","unstructured":"Jensenius JC, Siersted HC, Johnstone AP. Quantitation of human immunoglobulin by semiautomatic polyethylene glycol precipitation radioimmunoassays. Estimation of circulating immune complexes and immunoglobulin synthesized in vitro. J Immunol Methods 1983; 56: 19-32.","journal-title":"J Immunol Methods"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1159\/000233819","article-title":"Humoral immunity to dietary antigens in healthy adults. Occurrence, isotype and IgG subclass distribution of serum antibodies to protein antigens","volume":"77","author":"Husby, S.; Oxelius, V.-A.; Teisner, B.; Jensenius, J.C.; Svehag, S.-E.","year":"1985","unstructured":"Husby S, Oxelius V-A, Teisner B, Jensenius JC, Svehag S-E. Humoral immunity to dietary antigens in healthy adults. Occurrence, isotype and IgG subclass distribution of serum antibodies to protein antigens. Int Arch Allergy Appl Immunol 1985; 77: 416-22.","journal-title":"Int Arch Allergy Appl Immunol"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1111\/j.1398-9995.1986.tb00316.x","article-title":"Humoral immunity to dietary antigens in atopic dermatitis I Biotin-avidin amplified ELISA-analysis for serum IgG antibodies","volume":"41","author":"Husby, S.; Schultz Larsen, F.; Petersen, P.H.; Svehag, S.-E.","year":"1986","unstructured":"Husby S, Schultz Larsen F, Petersen PH, Svehag S-E. Humoral immunity to dietary antigens in atopic dermatitis I Biotin-avidin amplified ELISA-analysis for serum IgG antibodies. Allergy 1986; 41: 379-85.","journal-title":"Allergy"},{"key":"ref_23","first-page":"531","article-title":"Uptake and transport of macromolecules by the intestine. Possible role","volume-title":"clinical disorders. Gastroenterology","volume":"67","author":"Walker, W.A.; Isselbacher, K.J.","year":"1974","unstructured":"Walker WA, Isselbacher KJ. Uptake and transport of macromolecules by the intestine. Possible role in clinical disorders. Gastroenterology 1974; 67: 531-50."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1111\/j.1365-3083.1986.tb02133.x","article-title":"Passage of undegraded dietary antigen into the blood of healthy adults. Further characterization of the kinetics of uptake and the size distribution of the antigen","volume":"24","author":"Husby, S.; Jensenius, J.C.; Svehag, S.-E.","year":"1986","unstructured":"Husby S, Jensenius JC, Svehag S-E. Passage of undegraded dietary antigen into the blood of healthy adults. Further characterization of the kinetics of uptake and the size distribution of the antigen. Scand J Immunol 1986; 24: 447-55.","journal-title":"Scand J Immunol"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1111\/j.1365-2222.1977.tb01489.x","article-title":"A study of serum antibodies to isolated milk proteins and ovalbumin in infants and children","volume":"7","author":"May, C.D.; Remigio, L.; Feldman, J.; Bock, S.A.; Carr, R.J.","year":"1977","unstructured":"May CD, Remigio L, Feldman J, Bock SA, Carr RJ. A study of serum antibodies to isolated milk proteins and ovalbumin in infants and children. Clin Allergy 1977; 7: 583-95.","journal-title":"Clin Allergy"},{"key":"ref_26","first-page":"402","article-title":"Evidence for complement-binding immune complexes in adult coeliac disease, Crohn's disease and ulcerative colitis","volume":"ii:","author":"Doe WF, Booth CC, Brown DL","year":"1973","unstructured":"Doe WF, Booth CC, Brown DL. Evidence for complement-binding immune complexes in adult coeliac disease, Crohn's disease and ulcerative colitis. Lancet 1973; ii: 402.","journal-title":"Lancet"},{"key":"ref_27","first-page":"487","article-title":"Multiple immune complexes and hypocomplementemia in dermatitis herpetiformis and coeliac disease","volume":"ii:","author":"Mohammed, J.; Holborow, E.J.; Fry, L.; Thompson, B.R.; Hoffbrand, A.V.; Stewart, J.S.","year":"1976","unstructured":"Mohammed J, Holborow EJ, Fry L, Thompson BR, Hoffbrand AV, Stewart JS. Multiple immune complexes and hypocomplementemia in dermatitis herpetiformis and coeliac disease. Lancet 1976; ii: 487-90.","journal-title":"Lancet"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"892","DOI":"10.1016\/S0140-6736(84)90654-8","article-title":"Demonstration of tissue 90 kD glycoprotein as antigen in circulating IgG immune complexes in dermatitis herpetiformis and coeliac disease","volume":"ii:","author":"Maury, C.P.J.; Teppo, A.-M.","year":"1984","unstructured":"Maury CPJ, Teppo A-M. Demonstration of tissue 90 kD glycoprotein as antigen in circulating IgG immune complexes in dermatitis herpetiformis and coeliac disease. Lancet 1984; ii: 892-4.","journal-title":"Lancet"}],"container-title":["Gut"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/gut.28.9.1062","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T05:25:09Z","timestamp":1764998709000},"score":1,"resource":{"primary":{"URL":"https:\/\/gut.bmj.com\/lookup\/doi\/10.1136\/gut.28.9.1062"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,9]]},"references-count":28,"journal-issue":{"issue":"9","published-online":{"date-parts":[[1987,9,1]]},"published-print":{"date-parts":[[1987,9]]}},"alternative-id":["10.1136\/gut.28.9.1062"],"URL":"https:\/\/doi.org\/10.1136\/gut.28.9.1062","relation":{},"ISSN":["0017-5749","1468-3288"],"issn-type":[{"value":"0017-5749","type":"print"},{"value":"1468-3288","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,9]]}}}