{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T07:55:12Z","timestamp":1765007712722,"version":"3.46.0"},"reference-count":29,"publisher":"BMJ","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"published-print":{"date-parts":[[1987,9]]},"abstract":"<jats:p>Liver membrane vesicles were prepared from operative liver biopsies from six patient volunteers undergoing abdominal surgery for non-hepatic disease. Neutrophils were extracted from their blood. The liver membrane vesicles were exposed to 1 mmol\/l acetaldehyde with or without reduction of the resultant adducts formed. The production of superoxide anion by the neutrophils upon exposure to the liver membrane vesicles prepared from the same patient was assessed by measuring the rate of cytochrome c reduction before and after the addition of superoxide dismutase. Preincubation with acetaldehyde significantly increased superoxide production in response to both the reduced (from 35.5 +\/- 7.1 nmol O2-\/10(8) cells\/min to 128 +\/- 25, mean +\/- SEM, p less than 0.01) and the non-reduced liver cell membranes (from 17.2 +\/- 4.3 to 81 +\/- 17, p less than 0.01); 1 mmol\/l acetaldehyde alone caused no superoxide production. Neutrophil free radical production in response to acetaldehyde altered hepatocyte membranes could be an important mechanism of cellular injury in acute alcoholic hepatitis.<\/jats:p>","DOI":"10.1136\/gut.28.9.1157","type":"journal-article","created":{"date-parts":[[2007,9,18]],"date-time":"2007-09-18T17:41:31Z","timestamp":1190137291000},"page":"1157-1161","source":"Crossref","is-referenced-by-count":28,"title":["Free radical generation by neutrophils: a potential mechanism of cellular injury in acute alcoholic hepatitis."],"prefix":"10.1136","volume":"28","author":[{"given":"A J","family":"Williams","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R E","family":"Barry","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1987,9,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1136\/gut.25.4.412","article-title":"Acetaldehyde binds to liver cell membranes without affecting membrane function","volume":"25","author":"Barry, R.E.; McGivan, J.D.; Hayes, M.","year":"1984","unstructured":"Barry RE, McGivan JD, Hayes M. 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Br Med Bull 1982; 38: 63-5.","journal-title":"Br Med Bull"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1172\/JCI108635","article-title":"Evidence that the superoxide generating system of human leukocytes is associated with the cell surface","volume":"59","author":"Goldstein, I.M.; Cerquerira, M.; Lind, S.; Kaplan, H.B.","year":"1977","unstructured":"Goldstein IM, Cerquerira M, Lind S, Kaplan HB. Evidence that the superoxide generating system of human leukocytes is associated with the cell surface. J Clin Invest 1977; 59: 249-54.","journal-title":"J Clin Invest"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/S0140-6736(82)91032-7","article-title":"Could superoxide cause cirrhosis?","volume":"ii:","author":"Lewis, K.O.; Paton, A.","year":"1982","unstructured":"Lewis KO, Paton A. Could superoxide cause cirrhosis? 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Gut 1986; 27: A615."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1042\/cs0710313","article-title":"Superoxide production and degranulation of rat neutrophils in response to acetaldehyde altered liver cell membranes","volume":"71","author":"Williams, A.J.K.; Barry, R.E.","year":"1986","unstructured":"Williams AJK, Barry RE. Superoxide production and degranulation of rat neutrophils in response to acetaldehyde altered liver cell membranes. Clin Sci 1986; 71: 313-8.","journal-title":"Clin Sci"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1042\/bj2230733","article-title":"A procedure for the rapid isolation from rat liver of plasma membrane vesicles exhibiting Cant-transport and Ca>-ATPase activities","volume":"223","author":"Epping, R.J.; Bygrave, F.L.","year":"1984","unstructured":"Epping RJ, Bygrave FL. 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Analytical subcellular fractionation of human granulocytes with special reference to the localization of enzymes involved in microbicidal mechanisms. Clin Sci Mol Med 1977; 52: 429-42.","journal-title":"Clin Sci Mol Med"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"4359","DOI":"10.1016\/S0021-9258(17)30016-9","article-title":"Labelling of proteins by reductive methylation using sodium cyanborohydride","volume":"254","author":"Jentoft, N.; Dearborn, D.G.","year":"1979","unstructured":"Jentoft N, Dearborn DG. Labelling of proteins by reductive methylation using sodium cyanborohydride. J Biol Chem 1979; 254: 4359-65.","journal-title":"J Biol Chem"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1042\/bj2040479","article-title":"The enzymatic reduction and kinetics of oxidation of cytochrome b-245 of neutrophils","volume":"204","author":"Cross, A.R.; Higson, F.K.; Jones, O.T.G.","year":"1982","unstructured":"Cross AR, Higson FK, Jones OTG. The enzymatic reduction and kinetics of oxidation of cytochrome b-245 of neutrophils. Biochem J 1982; 204: 479-85.","journal-title":"Biochem J"},{"key":"ref_16","first-page":"447","article-title":"Response of superoxide anion production by guinea pig eosinophils to Free radical generation by neutrophils: a potential mechanism ofcellular injury in acute alcoholic hepatitis 1161 various soluble stimuli: comparison to neutrophils","volume":"241","author":"Yamashita, T.; Soneya, A.; Hara, E.","year":"1985","unstructured":"Yamashita T, Soneya A, Hara E. Response of superoxide anion production by guinea pig eosinophils to Free radical generation by neutrophils: a potential mechanism ofcellular injury in acute alcoholic hepatitis 1161 various soluble stimuli: comparison to neutrophils. Arch Biochem Biophys 1985; 241: 447-52.","journal-title":"Arch Biochem Biophys"},{"article-title":"Free radicals in biology and medicine","author":"Halliwell, B.; Gutteridge, J.M.C.","key":"ref_17","unstructured":"Halliwell B, Gutteridge JMC. Free radicals in biology and medicine. Clarendon Press: Oxford, 1985: 118."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/0098-2997(85)90001-9","article-title":"The importance of free radicals and catalytic metal ions in human disease","volume":"8","author":"Halliwell, B.; Gutteridge, J.M.C.","year":"1985","unstructured":"Halliwell B, Gutteridge JMC. The importance of free radicals and catalytic metal ions in human disease. Mol Aspects Med 1985; 8: 89-193.","journal-title":"Mol Aspects Med"},{"key":"ref_19","first-page":"1114","article-title":"Oxygen derived free radicals promote hepatic injury","volume-title":"the rat. 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Depressed hepatic glutathione and increased diene conjugates in alcoholic liver disease. Dig Dis Sci 1983; 28: 585-89.","journal-title":"Dig Dis Sci"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1111\/j.1530-0277.1983.tb05432.x","article-title":"Determinants of blood acetaldehyde level during ethanol oxidation in chronic alcoholics","volume":"7","author":"Nuutinen, H.; Lindros, K.O.; Salaspuro, M.","year":"1983","unstructured":"Nuutinen H, Lindros KO, Salaspuro M. Determinants of blood acetaldehyde level during ethanol oxidation in chronic alcoholics. Alcohol Clin Exp Res 1983; 7: 163-7.","journal-title":"Alcohol Clin Exp Res"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/0006-2952(77)90310-0","article-title":"The distribution and metabolism of acetaldehyde in rats during ethanol oxidation","volume":"26","author":"Erikson, C.J.P.; Sippel, H.W.","year":"1977","unstructured":"Erikson CJP, Sippel HW. The distribution and metabolism of acetaldehyde in rats during ethanol oxidation. Biochem Pharmacol 1977; 26: 241-7.","journal-title":"Biochem Pharmacol"},{"key":"ref_24","first-page":"3","article-title":"Alcoholic liver disease: pathobiology, epidemiology and clinical aspects","year":"1985","unstructured":"Alcoholic liver disease: pathobiology, epidemiology and clinical aspects. London: Edward Arnold, 1985; 3-40."},{"key":"ref_25","first-page":"71","article-title":"Membrane flow and interconversions among endomembranes","volume":"559","author":"Morre, D.J.; Kartenbech, J.; Franke, W.W.","year":"1979","unstructured":"Morre DJ, Kartenbech J, Franke WW. Membrane flow and interconversions among endomembranes. 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J Lab Clin Med 1985; 105: 5-10.","journal-title":"J Lab Clin Med"},{"key":"ref_28","article-title":"Essential immunology","author":"Roitt, I.","unstructured":"Roitt I. Essential immunology. 5th ed. Oxford: Blackwell Scientific Publications, 1984: 168.","edition":"5th ed"},{"key":"ref_29","first-page":"725","article-title":"Oxygen radical production by horse and pig neutrophils induced by a range of crystals","volume":"11","author":"Higson, F.K.; Jones, O.T.G.","year":"1984","unstructured":"Higson FK, Jones OTG. Oxygen radical production by horse and pig neutrophils induced by a range of crystals. 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