{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T13:55:36Z","timestamp":1775656536426,"version":"3.50.1"},"reference-count":29,"publisher":"BMJ","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"published-print":{"date-parts":[[1988,4]]},"abstract":"<jats:p>Seventy seven sera with antimitochondrial antibody exhibiting the M2-M4 pattern in immunofluorescence (56 from primary biliary cirrhosis (PBC), 21 from non-primary biliary cirrhosis patients) were studied by the combined use of Western immunoblotting with beef heart mitochondria and an enzyme linked immunosorbent assay (ELISA) with beef heart submitochondrial particles. Forty seven sera (10 without autoantibodies and 37 with different auto-antibodies) were included as controls. By immunoblotting, seven mitochondrial peptides reacting with antimitochondrial antibody positive sera were detected. These were of molecular weight 74 kD, 58 kD, 55 kD, 52 kD, 51 kD, 46 kD, and 43 kD. All primary biliary cirrhosis sera and 71% of antimitochondrial antibody-positive non-primary biliary cirrhosis sera reacted with one or more of these peptides, while none of the 47 antimitochondrial antibody negative sera reacted in immunoblotting. The 74 kD band was the most frequently detected (84% of primary biliary cirrhosis and 57% of non-primary biliary cirrhosis cases). All the primary biliary cirrhosis sera which failed to react with this peptide, showed a positive reaction with that of molecular weight 52 kD. 67\/77 (87%) immunofluorescence antimitochondrial antibody positive sera reacted in the ELISA test (93% of primary biliary cirrhosis and 71% of non-primary biliary cirrhosis cases). All the 47 immunofluorescence antimitochondrial antibody negative sera were confirmed negative by ELISA. The ELISA values correlated with the immunofluorescence titres (p less than 0.05). By comparison of the results obtained by these two techniques, it emerged that the ELISA test (using our preparation of submitochondrial particles) was not able to detect the antibody directed against the mitochondrial peptide of 52 kD, which thus seems to be different from the other specificities.<\/jats:p>","DOI":"10.1136\/gut.29.4.440","type":"journal-article","created":{"date-parts":[[2007,9,18]],"date-time":"2007-09-18T17:41:31Z","timestamp":1190137291000},"page":"440-447","source":"Crossref","is-referenced-by-count":20,"title":["Heterogeneity of antimitochondrial antibodies with the M2-M4 pattern by immunofluorescence as assessed by Western immunoblotting and enzyme linked immunosorbent assay."],"prefix":"10.1136","volume":"29","author":[{"given":"M","family":"Fusconi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I","family":"Ghadiminejad","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F B","family":"Bianchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H","family":"Baum","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G F","family":"Bottazzo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"Pisi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1988,4,1]]},"reference":[{"key":"ref_1","article-title":"Considering the anti-mitochondrial antibodies from primary biliary cirrhosis cases, 84% recognised the 74 kD protein (often in association with the 52 kD band), while the remaining 16% were negative for the 74 kD band but invariably reacted with the 52 kD one. In the ELISA test the intensity of the reactions correlated with all of the bands detected by immunoblotting except for that of 52 kD. Moreover, the correlation between the ELISA results and the immunofluorescence titres significantly increases (from p<O05 to p<O-OO1) when sera reacting with this latter protein only were excluded","unstructured":"Considering the anti-mitochondrial antibodies from primary biliary cirrhosis cases, 84% recognised the 74 kD protein (often in association with the 52 kD band), while the remaining 16% were negative for the 74 kD band but invariably reacted with the 52 kD one. In the ELISA test the intensity of the reactions correlated with all of the bands detected by immunoblotting except for that of 52 kD. Moreover, the correlation between the ELISA results and the immunofluorescence titres significantly increases (from p<O05 to p<O-OO1) when sera reacting with this latter protein only were excluded. Thus, it is tempting to conclude that the 52 kD peptide is the target of a mitochondrial specificity different from that at 74 kD MW."},{"key":"ref_2","first-page":"740","article-title":"immunofluorescence antimitochondrial antibody Lancet","volume":"i:","year":"1970","unstructured":"immunofluorescence antimitochondrial antibody Lancet 1970; i: 740-4."},{"key":"ref_3","first-page":"827","article-title":"positive sera are different from","volume":"i:","author":"Walker, J.G.; Doniach, D.; Roitt, I.M.; Sherlock, S.","year":"1965","unstructured":"positive sera are different from all of the others, 2 Walker JG, Doniach D, Roitt IM, Sherlock S. Serowhich are immunoblotting reactive. The negativity of this test could be caused by a denaturing effect of the immunoblotting procedure on the antigen(s). The clinical significance of antimitochondrial antibody positivity in patients without evidence of a liver disorder'3 '4 is, at present, controversial. In this context, it seems relevant that six of our 21 antilogical tests in diagnosis of primary biliary cirrhosis. Lancet 1965; i: 827-31.","journal-title":"Lancet; Serowhich are immunoblotting reactive. The negativity of this test could be caused by a denaturing effect of the immunoblotting procedure on the antigen(s). The clinical significance of antimitochondrial antibody positivity in patients without evidence of a liver disorder'3 '4"},{"key":"ref_4","first-page":"68","article-title":"Heterogeneity of antimitochondrial antibodies: characterization and separation of the antigen associated with the pseudolupus erythematosus syndrome. Clin Exp Immunol","volume":"37","author":"Sayers, T.Y.; Binder, T.; Berg, P.A.","year":"1979","unstructured":"Sayers TY, Binder T, Berg PA. Heterogeneity of antimitochondrial antibodies: characterization and separation of the antigen associated with the pseudolupus erythematosus syndrome. Clin Exp Immunol 1979; 37: 68-75."},{"key":"ref_5","first-page":"1329","volume":"ii:","author":"antibodies","year":"1980","unstructured":"antibodies. Lancet 1980; ii: 1329-32.","journal-title":"Lancet"},{"key":"ref_6","first-page":"357","article-title":"A new pattern of non-organ and non-species-specific antiorganelle antibody detected by immunofluorescence: the mitochondrial antibody number 5. Clin Exp Immunol","volume":"31","author":"Labro, M.T.; Andrieu, M.C.; Weber, M.; Homberg, J.C.","year":"1978","unstructured":"Labro MT, Andrieu MC, Weber M, Homberg JC. A new pattern of non-organ and non-species-specific antiorganelle antibody detected by immunofluorescence: the mitochondrial antibody number 5. Clin Exp Immunol 1978; 31: 357-66."},{"key":"ref_7","first-page":"93","article-title":"A new antimitochondrial antibody (antiM6) in iproniazid-induced hepatitis. Clin Exp Immunol From the present data it","volume":"47","author":"Homberg, J.C.; Stelly, N.; Adreis, I.; Abuaf, N.; Saadoun, F.; Andre, J.F.","year":"1982","unstructured":"Homberg JC, Stelly N, Adreis I, Abuaf N, Saadoun F, Andre JF. A new antimitochondrial antibody (antiM6) in iproniazid-induced hepatitis. Clin Exp Immunol From the present data it can be concluded that 1982; 47: 93-102."},{"key":"ref_8","first-page":"283","article-title":"The lower sensitivity is in part due to the fact that submitochondrial particles seem to be poor in the antigens cross-reacting with the 52 kD peptide. On the other hand the immunoblotting test increases the nosological specificity of the sensitive immunotion of organ specific antibodies against heart mitochondria (anti M7) in sera from patients with some forms of heart disease. Clin Exp Immunol 1985; 58","unstructured":"The lower sensitivity is in part due to the fact that submitochondrial particles seem to be poor in the antigens cross-reacting with the 52 kD peptide. On the other hand the immunoblotting test increases the nosological specificity of the sensitive immunotion of organ specific antibodies against heart mitochondria (anti M7) in sera from patients with some forms of heart disease. Clin Exp Immunol 1985; 58: 283-92."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1002\/hep.1840060402","article-title":"Characterization and clinical relevance of a new complementfixing antibody - anti-M8 - in patients with primary biliary cirrhosis","volume":"6","author":"Weber, P.; Brenner, J.; Stechemesser, E.","year":"1986","unstructured":"Weber P, Brenner J, Stechemesser E, et al. Characterization and clinical relevance of a new complementfixing antibody - anti-M8 - in patients with primary biliary cirrhosis. Hepatology 1986; 6: 553-9.","journal-title":"Hepatology"},{"key":"ref_10","first-page":"9","article-title":"fluorescence test for the diagnosis of primary biliary","volume-title":"Anticirrhosis because it is able to exclude immunomitochondrial antibodies in primary biliary cirrhosis. J fluorescence positive sera without immunoblotting reactivities, and to discriminate, among immunofluorescence positive sera, according to different immunoblotting patterns. It is well known that there is a partial clinical, and sometimes even histological, overlap between primary biliary cirrhosis and cases of chronic liver Hepatol 1986; 2","author":"Berg, P.A.; Klein, R.; Linderborn-Fotinos, J.","unstructured":"fluorescence test for the diagnosis of primary biliary 9 Berg PA, Klein R, Linderborn-Fotinos J. Anticirrhosis because it is able to exclude immunomitochondrial antibodies in primary biliary cirrhosis. 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Relationship to immunofluorescent and precipitating antibodies. Clin Exp Immunol 1985; 59","unstructured":"chondrial protein antigens assessed by enzyme linked immunosorbent assay for antimitochondrial antibodies. Relationship to immunofluorescent and precipitating antibodies. Clin Exp Immunol 1985; 59: 604-12."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1136\/gut.22.2.136","article-title":"Is mitochondrial antibody diagnostic of primary biliary cirrhosis?","volume":"22","author":"Munoz, L.E.; Thomas, H.C.; Scheuer, P.J.; Doniach, D.; Sherlock, S.","year":"1981","unstructured":"Munoz LE, Thomas HC, Scheuer PJ, Doniach D, Sherlock S. Is mitochondrial antibody diagnostic of primary biliary cirrhosis? Gut 1981; 22: 136-40.","journal-title":"Gut"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"814","DOI":"10.1136\/gut.23.10.814","article-title":"What does Marco Fusconi was recipient of a grant from Terme di antimitochondrial antibody mean?","volume":"23","author":"Triger, D.R.; Charlton, C.A.C.; Ward, A.M.","year":"1982","unstructured":"Triger DR, Charlton CAC, Ward AM. What does Marco Fusconi was recipient of a grant from Terme di antimitochondrial antibody mean? Gut 1982; 23: 814-8.","journal-title":"Gut"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/0098-2997(85)90007-X","article-title":"The PBC-specific antigen","volume":"8","author":"Baum, H.; Palmer, C.","year":"1985","unstructured":"Baum H, Palmer C. The PBC-specific antigen. 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Frequency and significance of antimitochondrial antibodies in severe chronic active hepatitis. Dig Dis Sci 1986; 31: 705-11.","journal-title":"Dig Dis Sci"}],"container-title":["Gut"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/gut.29.4.440","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T06:14:31Z","timestamp":1765606471000},"score":1,"resource":{"primary":{"URL":"https:\/\/gut.bmj.com\/lookup\/doi\/10.1136\/gut.29.4.440"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,4]]},"references-count":29,"journal-issue":{"issue":"4","published-online":{"date-parts":[[1988,4,1]]},"published-print":{"date-parts":[[1988,4]]}},"alternative-id":["10.1136\/gut.29.4.440"],"URL":"https:\/\/doi.org\/10.1136\/gut.29.4.440","relation":{},"ISSN":["0017-5749","1468-3288"],"issn-type":[{"value":"0017-5749","type":"print"},{"value":"1468-3288","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,4]]}}}