{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T19:35:49Z","timestamp":1757619349307,"version":"3.44.0"},"reference-count":33,"publisher":"BMJ","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"published-print":{"date-parts":[[1989,3]]},"abstract":"<jats:p>The in vitro secretion of immunoglobulins by small intestinal lymphocytes isolated from 47 patients with normal histology and 23 patients with treated and untreated coeliac disease was examined using an enzyme linked immunosorbent assay. In control patients, duodenal lymphocytes spontaneously secreted higher levels of IgM than jejunal lymphocytes (p less than 0.05). Significantly higher levels of both IgA (p less than 0.05) and IgM (p less than 0.001) were secreted by jejunal lymphocytes of 10 patients with untreated coeliac disease than cells isolated from normal jejunal tissue. IgM and IgA secretion by duodenal lymphocytes isolated from control patients was increased in a dose dependent manner by coculture with autologous peripheral blood T lymphocytes. This effect was not observed with jejunal lymphocytes of control or treated coeliac patients. Peripheral T-cells of untreated coeliac patients, however, showed significant helper effects (p less than 0.05) for IgM and IgA secretion by autologous jejunal lymphocytes. The results suggest that jejunal lymphocytes of patients with untreated coeliac disease show major differences in their capacity to synthesise and secrete immunoglobulins in vitro and the enhanced secretion might result from changes in T-cell immunoregulatory function.<\/jats:p>","DOI":"10.1136\/gut.30.3.347","type":"journal-article","created":{"date-parts":[[2007,9,18]],"date-time":"2007-09-18T17:41:31Z","timestamp":1190137291000},"page":"347-354","source":"Crossref","is-referenced-by-count":18,"title":["Immunoglobulin secretion by isolated intestinal lymphocytes: spontaneous production and T-cell regulation in normal small intestine and in patients with coeliac disease."],"prefix":"10.1136","volume":"30","author":[{"given":"J E","family":"Crabtree","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R V","family":"Heatley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M L","family":"Losowsky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1989,3,1]]},"reference":[{"key":"ref_1","first-page":"75","article-title":"Gluten-sensitive enteropathy, an abnormal immunologic response of the gastrointestinal tract to a dietary protein","volume-title":"Gastrointestinal immunity for the clinician","author":"Strober, W.","year":"1985","unstructured":"Strober W. Gluten-sensitive enteropathy, an abnormal immunologic response of the gastrointestinal tract to a dietary protein. In: Shortner RG. Kirsner JB. eds. Gastrointestinal immunity for the clinician. Orlando: Grove & Stratton Inc, 1985: 75-112."},{"key":"ref_2","first-page":"437","article-title":"The microenvironment of coeliac disease: T cell phenotypes and expression of the T2 'T blast' antigen by small bowel lymphocytes","volume":"60","year":"1985","unstructured":"Malizia G, Trejdosiewicz LK. Wood GM. Howdle PD. Janossy G, Losowsky MS. The microenvironment of coeliac disease: T cell phenotypes and expression of the T2 'T blast' antigen by small bowel lymphocytes. Clin Fxp Immunol 1985; 60: 437-46.","journal-title":"Clin Fxp Immunol"},{"key":"ref_3","first-page":"177","article-title":"Immunoperoxidase demonstration of the cellular composition of the normal and coeliac small bowel. Clin Exp Immunol","volume":"68","author":"Kelly, J.; O'Farrelly, C.; Weir, O.'Mahoney C.; DG, Feighery; C.","year":"1987","unstructured":"Kelly J, O'Farrelly C, O'Mahoney C. Weir DG, Feighery C. Immunoperoxidase demonstration of the cellular composition of the normal and coeliac small bowel. Clin Exp Immunol 1987; 68: 177-88."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1136\/gut.15.5.371","article-title":"Jejunal mucosal immunoglobulincontaining cells and jejunal fluid immunoglobulins in adult coeliac disease and dermatitis herpetiformis","volume":"15","author":"ML, Lancaster-Smith M.Kumar P.Marks R.Clark; AM, Dawson","year":"1974","unstructured":"Lancaster-Smith M. Kumar P. Marks R. Clark ML, Dawson AM. Jejunal mucosal immunoglobulincontaining cells and jejunal fluid immunoglobulins in adult coeliac disease and dermatitis herpetiformis. Gut 1974; 15:371-6.","journal-title":"Gut"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1080\/00365521.1977.12031127","article-title":"Fausa 0. Immunoglobulins in jejunal mucosa and serum from patients with adult coeliac disease","volume":"12","author":"Bakelien, K.; Brandtzaeg, P.","year":"1977","unstructured":"Bakelien K, Brandtzaeg P. Fausa 0. Immunoglobulins in jejunal mucosa and serum from patients with adult coeliac disease. Scand J Gastroenterol 1977; 12: 149-59.","journal-title":"Scand J Gastroenterol"},{"key":"ref_6","first-page":"123","article-title":"Jejunal plasma cells and in vitro immunoglobulin production in adult coeliac disease","volume":"69","author":"Wood, G.M.; Howdle, P.D.; Trejdosiewicz, L.K.; Losowsky, M.S.","year":"1987","unstructured":"Wood GM, Howdle PD, Trejdosiewicz LK, Losowsky MS. Jejunal plasma cells and in vitro immunoglobulin production in adult coeliac disease. Clin Exp Immunol 1987; 69: 123-32.","journal-title":"Clin Exp Immunol"},{"key":"ref_7","first-page":"173","article-title":"Morphometric analysis of small intestinal mucosa. 2. Determination of lamina propria volumes; plasma cell and neutrophil populations within control and coeliac disease mucosae","volume":"403","year":"1984","unstructured":"Dhesi 1, Marsh MN, Kelly C, Crowe P. Morphometric analysis of small intestinal mucosa. 2. Determination of lamina propria volumes; plasma cell and neutrophil populations within control and coeliac disease mucosae. Virclhows Arch [Pathol Anatl 1984; 403: 173-80.","journal-title":"Virclhows Arch [Pathol Anatl"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1097\/00005176-198302010-00008","article-title":"Quantification of immunoglobulins after organ culture of human duodenal mucosa","volume":"2","author":"Fluge, G.; Aksnes, L.","year":"1983","unstructured":"Fluge G, Aksnes L. Quantification of immunoglobulins after organ culture of human duodenal mucosa. J Pediatr Gastroenterol Nutr 1983; 2: 62-70.","journal-title":"J Pediatr Gastroenterol Nutr"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.1136\/gut.27.10.1151","article-title":"Immunoglobulin production by coeliac biopsies in organ culture","volume":"27","year":"1986","unstructured":"Wood GM, Shires S, Howdle PD. Losowsky MS. Immunoglobulin production by coeliac biopsies in organ culture. Gut 1986; 27: 1151-60.","journal-title":"Gut"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1002\/path.1711210405","article-title":"Jejunal mucosal immunoglobulins and complement in untreated coeliac disease","volume":"121","year":"1977","unstructured":"Scott BB, Scott DG. Losowsky MS. Jejunal mucosal immunoglobulins and complement in untreated coeliac disease. J Pathol 1977; 121: 219-23.","journal-title":"J Pathol"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"966","DOI":"10.1172\/JCI108719","article-title":"Isolation and functional characterisation of human intestinal mucosal lymphoid cells","volume":"59","author":"MA, Bull D.M.Bookman","year":"1977","unstructured":"Bull DM. Bookman MA. Isolation and functional characterisation of human intestinal mucosal lymphoid cells. J Clin Invest 1977; 59: 966-74.","journal-title":"J Clin Invest"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"898","DOI":"10.1136\/gut.19.10.898","article-title":"Preparation of lymphoid cells from small specimens of human gastrointestinal mucosa","volume":"19","author":"Crofton, R.W.; Cochrane, C.; McClelland, D.B.L.","year":"1978","unstructured":"Crofton RW, Cochrane C, McClelland DBL. Preparation of lymphoid cells from small specimens of human gastrointestinal mucosa. 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C\/in Exp Immunol","volume":"73","author":"Smart, C.J.; Trejdosiewicz, L.K.; Heatley, Badr-el-Din S.; R.V.","year":"1988","unstructured":"Smart CJ, Trejdosiewicz LK, Badr-el-Din S. Heatley RV. T lymphocytes of the human colonic mucosa: functional and phenotypic analysis. C\/in Exp Immunol 1988; 73: 63-9."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1084\/jem.136.2.207","article-title":"Surface markers on human T and B lymphocytes. 1. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells","volume":"136","author":"Jondall, M.; Holm, G.; Wigzell, H.","year":"1972","unstructured":"Jondall M, Holm G, Wigzell H. Surface markers on human T and B lymphocytes. 1. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells. J Fxp Med 1972; 136: 207-15.","journal-title":"J Fxp Med"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/0022-1759(87)90470-4","article-title":"ELISA for measurement of secretory IgA distinct from monomeric IgA","volume":"97","author":"Wood, G.M.; Trejdosiewicz, L.K.; Losowsky, M.S.","year":"1987","unstructured":"Wood GM, Trejdosiewicz LK, Losowsky MS. ELISA for measurement of secretory IgA distinct from monomeric IgA. J Immunol Methods 1987b; 97: 269-74.","journal-title":"J Immunol Methods"},{"key":"ref_19","first-page":"1","article-title":"Regulation of human B lymphocyte activation, proliferation, and differentiation","volume":"40","author":"Jelinik, D.J.; Lipsky, P.J.","year":"1987","unstructured":"Jelinik DJ, Lipsky PJ. Regulation of human B lymphocyte activation, proliferation, and differentiation. 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Gasitroenterology 1981,; 81: 844-52.","journal-title":"Gasitroenterology"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/0016-5085(86)90572-X","article-title":"Altered patrens of secretion of monomeric IgA and IgA subclass I by intestinal mononuciear cells in inflammatory bowel disease","volume":"91","author":"MacDermott, R.P.; Nash, G.S.; Bertovich, M.J.; etal","year":"1986","unstructured":"MacDermott RP, Nash GS, Bertovich MJ, etal. Altered patrens of secretion of monomeric IgA and IgA subclass I by intestinal mononuciear cells in inflammatory bowel disease. Gastroenterology 1986; 91: 379-85.","journal-title":"Gastroenterology"},{"key":"ref_22","first-page":"7","article-title":"Evidence for regulation of human colonic mucosal immunoglobulin secretion by intestinal lymphoid cells","volume":"22","author":"RV, Danis V.A.Heatley","year":"1987","unstructured":"Danis VA. Heatley RV. Evidence for regulation of human colonic mucosal immunoglobulin secretion by intestinal lymphoid cells. J Clin Lab Iminunol 1987; 22: 7-11.","journal-title":"J Clin Lab Iminunol"},{"key":"ref_23","first-page":"118","article-title":"The B cell system","volume-title":"allergy and intolerance","author":"Brandtzaeg, P.","year":"1987","unstructured":"Brandtzaeg P. The B cell system. In: Brostoff J, Challacombe SJ, eds. Food allergy and intolerance. Sussex: Bailliere Tindall, 1987: 118-55."},{"key":"ref_24","first-page":"217","article-title":"Deficiency of M-globulin in coeliac disease","volume":"i:","author":"CW, Hobbs JR. Hepner","year":"1968","unstructured":"Hobbs JR. Hepner CW. Deficiency of M-globulin in coeliac disease. Lancet 1968; i: 217-20.","journal-title":"Lancet"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1136\/gut.22.3.169","article-title":"Janossy G6 Jewell DP. Immunohistological characterisation of intraepithelial lymphocytes of the human gastrointestinal tract","volume":"22","author":"Selby, W.S.","year":"1981","unstructured":"Selby WS, Janossy G6 Jewell DP. Immunohistological characterisation of intraepithelial lymphocytes of the human gastrointestinal tract. Gut 1981; 22: 169-76.","journal-title":"Gut"},{"key":"ref_26","first-page":"371","article-title":"Coeliac disease","volume-title":"Immunopatlhology of the small intestine","author":"Marsh, M.N.","year":"1987","unstructured":"Marsh MN. Coeliac disease. In: Marsh MN, ed. Immunopatlhology of the small intestine. Chichester: John Wiley. 1987: 371-99."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1136\/gut.25.1.32","article-title":"Intestinal lymphocyte subpopulations in inflammatory bowel disease: an analysis by immunohistological and cell isolation techniques","volume":"25","author":"Selby, W.S.; Jewell, Janossy G.Bofill M.; D.P.","year":"1984","unstructured":"Selby WS, Janossy G. Bofill M. Jewell DP. Intestinal lymphocyte subpopulations in inflammatory bowel disease: an analysis by immunohistological and cell isolation techniques. Gut 1984; 25: 32-40.","journal-title":"Gut"},{"issue":"1","key":"ref_28","first-page":"190","article-title":"Human antigenspecific IgA responses in blood and secondary lymphoid tissue: an analysis of help and suppression","volume":"132","author":"McCaughan, G.W.; Adams, E.; Basten, A.","year":"1984","unstructured":"McCaughan GW, Adams E, Basten A. Human antigenspecific IgA responses in blood and secondary lymphoid tissue: an analysis of help and suppression. J Immuniol 1984; 132: 1 190-6.","journal-title":"J Immuniol"},{"key":"ref_29","first-page":"1533","article-title":"Contrasuppression: a novel immunoregulatory activity","volume":"153","author":"Gershon, R.K.; Eardley, D.D.; Durum, S.","year":"1981","unstructured":"Gershon RK, Eardley DD, Durum S. et al. Contrasuppression: a novel immunoregulatory activity. J E:rp Med 1981; 153: 1533-46.","journal-title":"J E:rp Med"},{"key":"ref_30","first-page":"713","article-title":"Separation and characterization of a subset of human T8+ cells which function as antigen-presenting and contrasuppressor cells","volume":"54","author":"Lehner, T.; Avery, J.; Jones, T.","year":"1985","unstructured":"Lehner T, Avery J, Jones T. Separation and characterization of a subset of human T8+ cells which function as antigen-presenting and contrasuppressor cells. Immunology 1985; 54: 713-22.","journal-title":"Immunology"},{"key":"ref_31","first-page":"219","article-title":"Lymphocyte subpopulations in the human small intestine. The findings in normal mucosa and in the mucosa of patients with adult coeliac disease. Clin Exp Immunol 1983; 52","author":"Selby, W.S.; Bofill, Janossy G.; M, Jewell; D.P.","unstructured":"Selby WS, Janossy G. Bofill M, Jewell DP. Lymphocyte subpopulations in the human small intestine. The findings in normal mucosa and in the mucosa of patients with adult coeliac disease. Clin Exp Immunol 1983; 52: 219-28."},{"key":"ref_32","first-page":"113","article-title":"Relationship between human T-cell functional heterogenicity and human T-cell surface molecules. Immulnol Rei'","volume":"74","author":"L, Thomas Y.Rogoziuski; L, Chess","year":"1983","unstructured":"Thomas Y. Rogoziuski L, Chess L. Relationship between human T-cell functional heterogenicity and human T-cell surface molecules. Immulnol Rei' 1983; 74: 113-28."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1073\/pnas.79.3.889","article-title":"Microenvironmental immunoregulation: possible role of contrasuppressor cells in maintaining immune response in gut-associated lymphoid tissues","volume":"79","author":"Green, D.R.; Gold, J.; Gershon, St Martin S.; R, Gershon; R.K.","year":"1982","unstructured":"Green DR, Gold J, St Martin S. Gershon R, Gershon RK. Microenvironmental immunoregulation: possible role of contrasuppressor cells in maintaining immune response in gut-associated lymphoid tissues. 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