{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T07:01:01Z","timestamp":1765609261431,"version":"3.48.0"},"reference-count":32,"publisher":"BMJ","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"published-print":{"date-parts":[[1989,7]]},"abstract":"<jats:p>DNA image cytometry was performed on Feulgen stained sections from 91 biopsies obtained during prospective endoscopic surveillance of 55 patients with Barrett's oesophagus. Aneuploid cells were detected in specimens from six of these patients. Four subsequently developed dysplasia and adenocarcinoma but, in the other two, biopsies had been reported as showing specialised epithelium only, with no apparent dysplasia and no evidence of malignancy on clinical follow up to date. In two of the four patients who subsequently developed carcinoma, aneuploid cells were only found in biopsies showing overt dysplasia or carcinoma but in the two other patients aneuploid cells were present in biopsies taken early in the clinical course before any dysplasia had been identified on the original reports. The presence of aneuploid cells on cytometry of these 'benign' biopsies allowed us, on histological review, to identify areas of atypia which were interpreted as mild dysplasia. In this series aneuploidy was always associated with some morphological abnormality varying from mild dysplasia to frank carcinoma. Aneuploid cells were not shown in material from one patient who had an oesophagectomy for dysplasia or in biopsy material from four patients showing 'indefinite dysplasia'. DNA cytometry combines an objective assessment of epithelial atypia with the advantage of detecting rare cellular aneuploidy and the ability to correlate these events with morphology. It should assist in the more accurate diagnosis of dysplasia and prove useful in identifying those patients with Barrett's oesophagus who are at greater risk of subsequently developing malignancy.<\/jats:p>","DOI":"10.1136\/gut.30.7.899","type":"journal-article","created":{"date-parts":[[2007,9,18]],"date-time":"2007-09-18T17:41:31Z","timestamp":1190137291000},"page":"899-905","source":"Crossref","is-referenced-by-count":34,"title":["Value of DNA image cytometry in the prediction of malignant change in Barrett's oesophagus."],"prefix":"10.1136","volume":"30","author":[{"given":"P D","family":"James","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Atkinson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1989,7,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1002\/bjs.18003815005","article-title":"Chronic peptic ulcer of the oesophagus and 'oesophagitis'","volume":"38","author":"Barrett, N.R.","year":"1950","unstructured":"Barrett NR. 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Columnar-lined lower esophagus: An acquired lesion with malignant predisposition. Report on 140 cases of Barrett's esophagus with 12 adenocarcinomas. J Thorac Cardiovasc Surg 1975; 70: 826-34.","journal-title":"J Thorac Cardiovasc Surg"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1002\/bjs.1800750813","article-title":"Value of endoscopic surveillance in the detection of neoplastic change in Barrett's oesophagus","volume":"75","author":"Robertson, C.S.; Mayberry, J.F.; Nicholson, D.A.; James, P.D.; Atkinson, M.","year":"1988","unstructured":"Robertson CS, Mayberry JF, Nicholson DA, James PD, Atkinson M. Value of endoscopic surveillance in the detection of neoplastic change in Barrett's oesophagus. 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Correlation between mucin histochemistry, flow cytometry, and histologic diagnosis for predicting increased cancer risk","volume":"131","author":"Haggit, R.C.; Reid, B.J.; Rabinovitch, P.S.; Rubin, C.E.","year":"1988","unstructured":"Haggit RC, Reid BJ, Rabinovitch PS, Rubin CE. Barrett's esophagus. Correlation between mucin histochemistry, flow cytometry, and histologic diagnosis for predicting increased cancer risk. Am J Pathol 1988; 131: 53-61.","journal-title":"Am J Pathol"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1097\/00000478-198512000-00001","article-title":"Dysplasia in Barrett's esophagus. A clinicopathologic study of six patients","volume":"9","author":"Lee, R.G.","year":"1985","unstructured":"Lee RG. Dysplasia in Barrett's esophagus. A clinicopathologic study of six patients. Am J Surg Pathol 1985; 9: 845-52.","journal-title":"Am J Surg Pathol"},{"key":"ref_9","first-page":"1","article-title":"Barrett's esophagus. Correlation between flow cytometry and histology in detection of patients at risk for adenocarcinoma","volume":"93","year":"1987","unstructured":"Barrett's esophagus. Correlation between flow cytometry and histology in detection of patients at risk for adenocarcinoma. Gastroenterology 1987; 93: 1-11.","journal-title":"Gastroenterology"},{"key":"ref_10","first-page":"1012","article-title":"DNA content in Barrett's esophagus and esophageal malignancy","volume":"82","author":"McKinley, M.J.; Budman, D.R.; Grueneberg, D.; Bronzo, R.L.; Weissman, G.S.; Kahn, E.","year":"1987","unstructured":"McKinley MJ, Budman DR, Grueneberg D, Bronzo RL, Weissman GS, Kahn E. DNA content in Barrett's esophagus and esophageal malignancy. Am J Gastroenterol 1987; 82: 1012-5.","journal-title":"Am J Gastroenterol"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1056\/NEJM197608262950904","article-title":"The histologic spectrum of Barrett's esophagus","volume":"295","author":"Paull, A.; Trier, J.S.; Dalton, M.D.; Camp, R.C.; Loeb, P.; Goyal, R.K.","year":"1976","unstructured":"Paull A, Trier JS, Dalton MD, Camp RC, Loeb P, Goyal RK. The histologic spectrum of Barrett's esophagus. N Engl J Med 1976; 295: 476-80.","journal-title":"N Engl J Med"},{"key":"ref_12","article-title":"Intestinal metaplasia subtypes and cancer risk","author":"Filipe, M.I.; Jass, JR","year":"1986","unstructured":"Filipe MI, Jass JR. Intestinal metaplasia subtypes and cancer risk. In: Filipe MI, Jass JR, eds. Gastric carcinoma. Edinburgh: Churchill Livingstone, 1986."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1016\/S0046-8177(83)80175-0","article-title":"Dysplasia in inflammatory bowel disease: standardized classification with provisional clinical applications","volume":"14","author":"Riddell, R.H.; Goldman, H.; Ransohoff, D.F.","year":"1983","unstructured":"Riddell RH, Goldman H, Ransohoff DF, et al. Dysplasia in inflammatory bowel disease: standardized classification with provisional clinical applications. Hum Pathol 1983; 14: 931-68.","journal-title":"Hum Pathol"},{"key":"ref_14","first-page":"554","article-title":"Barrett's esophagus. Comparison of benign and malignant cases","author":"Skinner, D.B.; Walther, B.C.; Riddell, R.H.; Schmidt, H.; Iascone, C.; DeMeester, T.R.","year":"1983","unstructured":"Skinner DB, Walther BC, Riddell RH, Schmidt H, Iascone C, DeMeester TR. Barrett's esophagus. Comparison of benign and malignant cases. Ann Surg 1983; 198: 554-65.","journal-title":"Ann Surg"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1093\/ajcp\/87.3.301","article-title":"The relationship between columnar epithelial dysplasia and invasive adenocarcinoma arising in Barrett's esophagus","volume":"87","author":"Hamilton, SR, Smith; R.L.","year":"1987","unstructured":"Hamilton SR, Smith RL. The relationship between columnar epithelial dysplasia and invasive adenocarcinoma arising in Barrett's esophagus. Am J Clin Pathol 1987; 87: 301-12.","journal-title":"Am J Clin Pathol"},{"key":"ref_16","first-page":"307","article-title":"The Feulgen hydrolysis profiles a metastatic lung tumour in lymph node and a primary breast carcinoma","volume":"44","author":"Mays, R.G.W.","year":"1987","unstructured":"Mays RGW. The Feulgen hydrolysis profiles a metastatic lung tumour in lymph node and a primary breast carcinoma. Med Lab Sci 1987; 44: 307-11.","journal-title":"Med Lab Sci"},{"key":"ref_17","first-page":"1","article-title":"Algorithm for a DNAcytophotometric diagnosis and grading of malignancy","volume":"6","author":"Bocking, A.; Adler, C.P.; Common, H.H.; Hilgarth, M.; Granzen, B.; Auffermann, W.","year":"1984","unstructured":"Bocking A, Adler CP, Common HH, Hilgarth M, Granzen B, Auffermann W. Algorithm for a DNAcytophotometric diagnosis and grading of malignancy. Anal Quant Cytol 1984; 6: 1-8.","journal-title":"Anal Quant Cytol"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1016\/S0022-5223(19)40115-3","article-title":"Columnar cell-lined oesophagus: assessment of etiology and treatment. A 22 year experience","volume":"71","author":"Borrie, J.; Goldwater, L.","year":"1976","unstructured":"Borrie J, Goldwater L. Columnar cell-lined oesophagus: assessment of etiology and treatment. A 22 year experience. J Thorac Cardiovasc Surg 1976; 71: 825-34.","journal-title":"J Thorac Cardiovasc Surg"},{"key":"ref_19","first-page":"97","article-title":"Barrett's oesophagus: a clinical study of 52 patients","volume":"62","author":"Cooper, B.T.; Barbezat, G.O.","year":"1987","unstructured":"Cooper BT, Barbezat GO. Barrett's oesophagus: a clinical study of 52 patients. Q J Med 1987; 62: 97-108.","journal-title":"Q J Med"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1056\/NEJM198510033131404","article-title":"The incidence of adenocarcinoma in columnar-lined (Barrett's) esophagus","volume":"313","author":"Cameron, A.J.; Ott, B.J.; Payne, W.S.","year":"1985","unstructured":"Cameron AJ, Ott BJ, Payne WS. The incidence of adenocarcinoma in columnar-lined (Barrett's) esophagus. N Engl J Med 1985; 313: 857-9.","journal-title":"N Engl J Med"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1016\/0016-5085(84)90090-8","article-title":"Adenocarcinoma and Barrett's esophagus. An overrated risk?","volume":"87","author":"Spechler, S.J.; Robbins, A.H.; Rubins, H.B.","year":"1984","unstructured":"Spechler SJ, Robbins AH, Rubins HB, et al. Adenocarcinoma and Barrett's esophagus. An overrated risk? Gastroenterology 1984; 87: 927-33.","journal-title":"Gastroenterology"},{"key":"ref_22","first-page":"291","article-title":"The cost of suveillance for adenocarcinoma complicating Barrett's esophagus","volume":"83","author":"Achkar, E.; Carey, W.","year":"1988","unstructured":"Achkar E, Carey W. The cost of suveillance for adenocarcinoma complicating Barrett's esophagus. Am J Gastroenterol 1988; 83: 291-4.","journal-title":"Am J Gastroenterol"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"866","DOI":"10.1136\/jcp.34.8.866","article-title":"Mucin histochemistry of the columnar epithelium of the oesophagus: a retrospective study","volume":"34","author":"Jass, JR","year":"1981","unstructured":"Jass JR. Mucin histochemistry of the columnar epithelium of the oesophagus: a retrospective study. J Clin Pathol 1981; 34:866-70.","journal-title":"J Clin Pathol"},{"key":"ref_24","first-page":"1282","article-title":"Sulfated mucosubstances in Barrett's (columnar cell) esophageal mucosa","volume":"80","author":"Sheahan, D.G.; West, A.B.","year":"1981","unstructured":"Sheahan DG, West AB. Sulfated mucosubstances in Barrett's (columnar cell) esophageal mucosa. Gastroenterology 1981; 80: 1282.","journal-title":"Gastroenterology"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1136\/jcp.37.6.607","article-title":"Mucin histochemistry of the columnar epithelium of the oesophagus (Barrett's oesophagus): a prospective biopsy study","volume":"37","author":"Peuchmaur, M.; Potet, F.; Goldfain, D.","year":"1984","unstructured":"Peuchmaur M, Potet F, Goldfain D. Mucin histochemistry of the columnar epithelium of the oesophagus (Barrett's oesophagus): a prospective biopsy study. J Clin Pathol 1984; 37: 607-10.","journal-title":"J Clin Pathol"},{"key":"ref_26","first-page":"1552","article-title":"Criteria for dysplasia in Barrett's esophagus: a cooperative consensus study","volume":"88","author":"Reid, B.J.; Haggitt, R.C.; Rubin, C.E.","year":"1985","unstructured":"Reid BJ, Haggitt RC, Rubin CE, et al. Criteria for dysplasia in Barrett's esophagus: a cooperative consensus study. Gastroenterology 1985; 88: 1552.","journal-title":"Gastroenterology"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1038\/bjc.1987.152","article-title":"Flow cytometric analysis of the DNA content of gastric cancer","volume":"56","author":"Ballantyne, K.C.; James, P.D.; Robins, R.A.; Baldwin, R.W.; Hardcastle, J.D.","year":"1987","unstructured":"Ballantyne KC, James PD, Robins RA, Baldwin RW, Hardcastle JD. Flow cytometric analysis of the DNA content of gastric cancer. Br J Cancer 1987; 56: 52-4.","journal-title":"Br J Cancer"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/0016-5085(87)90334-9","article-title":"Flow cytometric analysis of deoxyribonucleic acid content in the gastrointestinal tract. Is aneuploidy more than a new play?","volume":"93","author":"Ahnen, D.J.","year":"1987","unstructured":"Ahnen DJ. Flow cytometric analysis of deoxyribonucleic acid content in the gastrointestinal tract. Is aneuploidy more than a new play? Gastroenterology 1987; 93: 197-203.","journal-title":"Gastroenterology"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1002\/cyto.990060512","article-title":"DNA image cytometry on machine-selected breast cancer cells and a comparison between flow cytometry and scanning cytophotometry","volume":"6","author":"Cornelisse, C.J.; Van Driel-Kulker, A.M.J.","year":"1985","unstructured":"Cornelisse CJ, Van Driel-Kulker AMJ. DNA image cytometry on machine-selected breast cancer cells and a comparison between flow cytometry and scanning cytophotometry. Cytometry 1985; 6: 471-7.","journal-title":"Cytometry"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1111\/j.1365-2559.1987.tb01899.x","article-title":"The DNA content in cancer and dysplasia in chronic ulcerative colitis","volume":"11","author":"Cuvelier, C.A.; Morson, B.C.; Roels, H.J.","year":"1987","unstructured":"Cuvelier CA, Morson BC, Roels HJ. The DNA content in cancer and dysplasia in chronic ulcerative colitis. Histopathology 1987; 11: 927-39.","journal-title":"Histopathology"},{"key":"ref_31","first-page":"485","article-title":"DNA profiles in mitotic cells from gastric adenomas","volume":"130","author":"Rubio, C.A.; Kato, Y.","year":"1988","unstructured":"Rubio CA, Kato Y. DNA profiles in mitotic cells from gastric adenomas. Am J Pathol 1988; 130: 485-8.","journal-title":"Am J Pathol"},{"key":"ref_32","first-page":"303","article-title":"Aneuploidy as a marker of minimal residual disease in leukaemia","volume":"8","author":"Walle, A.J.; Niedermayer, W.","year":"1985","unstructured":"Walle AJ, Niedermayer W. Aneuploidy as a marker of minimal residual disease in leukaemia. Cancer Detect Prev 1985; 8: 303-15.","journal-title":"Cancer Detect Prev"}],"container-title":["Gut"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/gut.30.7.899","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T06:51:51Z","timestamp":1765608711000},"score":1,"resource":{"primary":{"URL":"https:\/\/gut.bmj.com\/lookup\/doi\/10.1136\/gut.30.7.899"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,7]]},"references-count":32,"journal-issue":{"issue":"7","published-online":{"date-parts":[[1989,7,1]]},"published-print":{"date-parts":[[1989,7]]}},"alternative-id":["10.1136\/gut.30.7.899"],"URL":"https:\/\/doi.org\/10.1136\/gut.30.7.899","relation":{},"ISSN":["0017-5749","1468-3288"],"issn-type":[{"type":"print","value":"0017-5749"},{"type":"electronic","value":"1468-3288"}],"subject":[],"published":{"date-parts":[[1989,7]]}}}