{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T10:17:48Z","timestamp":1770718668910,"version":"3.49.0"},"reference-count":33,"publisher":"BMJ","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"published-print":{"date-parts":[[1990,6]]},"abstract":"<jats:p>The human intestinal adenovirus serotype 12 (Ad12) may be implicated in the pathogenesis of coeliac disease by virtue of immunological cross reactivity between epitopes shared by its early region E1b protein and A-gliadin. In the present study a synthetic dodecapeptide from the corresponding viral epitope (Ad12E1b, residues 384-395) was tested for its effect on peripheral blood mononuclear cells from 22 treated and eight untreated patients with coeliac disease, 22 healthy subjects, 11 patients with ulcerative colitis, and 11 patients with Crohn's disease by an indirect leucocyte migration inhibition assay. In addition, the effect of both the viral and the gliadin synthetic peptides was studied by proliferation and migration assays simultaneously performed in an unselected subgroup of 12 treated coeliac patients and 12 healthy subjects of the study. Coeliac patients with untreated disease showed no response to the viral peptide compared with treated patients (p greater than 0.1). Treated coeliac patients showed a significantly different response from healthy control subjects and control patients with disease (p less than 0.001) which was dependent on the concentration of the viral peptide. In the subgroup of the treated coeliac patients (n = 12) there was a significant correlation between the responses in the migration and the proliferation assay using either the viral (p less than 0.02) or the gliadin (p less than 0.005) peptide at the highest concentration (33.3 micrograms\/ml). Furthermore, the responses obtained using viral peptide correlated significantly with the responses obtained with gliadin peptide in both the migration (p less than 0.001) and the proliferation (p less than 0.001) assays. These results show that in coeliac patients there is pronounced cross reactivity at the level of T cell recognition between synthetic peptides derived from the Ad12 and A-gliadin. This antigenic cross reactivity may be involved in the pathogenesis of coeliac disease.<\/jats:p>","DOI":"10.1136\/gut.31.6.668","type":"journal-article","created":{"date-parts":[[2007,9,18]],"date-time":"2007-09-18T17:41:31Z","timestamp":1190137291000},"page":"668-673","source":"Crossref","is-referenced-by-count":27,"title":["Cellular hypersensitivity to a synthetic dodecapeptide derived from human adenovirus 12 which resembles a sequence of A-gliadin in patients with coeliac disease."],"prefix":"10.1136","volume":"31","author":[{"given":"G J","family":"Mantzaris","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J A","family":"Karagiannis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J D","family":"Priddle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D P","family":"Jewell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1990,6,1]]},"reference":[{"issue":"94","key":"ref_1","first-page":"477","article-title":"Starch gel electrophoresis of wheat gluten proteins with concentrated urea","volume":"1961","author":"Woychik, J.H.; Boundy, J.A.; Dimler, R.J.","unstructured":"Woychik JH, Boundy JA, Dimler RJ. Starch gel electrophoresis of wheat gluten proteins with concentrated urea. Arch Biochem Biophys 1961; 94: 477-82.","journal-title":"Arch Biochem Biophys"},{"key":"ref_2","first-page":"151","article-title":"Structure and properties of a-gliadins","volume":"29","author":"Kasarda, D.D.","year":"1980","unstructured":"Kasarda DD. Structure and properties of a-gliadins. Ann Tech Agnc 1980; 29: 151-3.","journal-title":"Ann Tech Agnc"},{"key":"ref_3","article-title":"Toxic proteins and peptides in celiac disease: relations to cereal genetics","volume-title":"nutrition and evolution","author":"Kasarda, D.D.","year":"1981","unstructured":"Kasarda DD. Toxic proteins and peptides in celiac disease: relations to cereal genetics. In: Walcher D, Kretchmer M, eds. Food, nutrition and evolution. New York: Masson, 1981: 201-16."},{"key":"ref_4","first-page":"4712","article-title":"Nucleic acid (cDNA) and amino acid sequences of a-type gliadins from wheat","volume-title":"(Triticum aestivum L). Proc Natl Acad Sci","volume":"81","author":"Kasarda, D.D.; Okita, T.W.; Bernardin, J.E.","year":"1984","unstructured":"Kasarda DD, Okita TW, Bernardin JE, et al. Nucleic acid (cDNA) and amino acid sequences of a-type gliadins from wheat (Triticum aestivum L). Proc Natl Acad Sci USA 1984; 81: 4712-6."},{"key":"ref_5","first-page":"162","article-title":"Histocompatibility antigens associated with coeliac disease","volume":"ii:","author":"Stokes, P.L.; Asquith, P.; Holmes, G.K.T.; MacKintosh, P.; Cooke, W.T.","year":"1972","unstructured":"Stokes PL, Asquith P, Holmes GKT, MacKintosh P, Cooke WT. Histocompatibility antigens associated with coeliac disease. Lancet 1972; ii: 162-4.","journal-title":"Lancet"},{"key":"ref_6","first-page":"1785","article-title":"Segregation analysis of HL-A antigens and haplotypes in 50 families of patients with celiac disease","volume":"5","author":"Albert, E.J.; Harms, K.; Wank, R.; Steinbauer-Rosenthal, I.; Schultz, S.","year":"1973","unstructured":"Albert EJ, Harms K, Wank R, Steinbauer-Rosenthal I, Schultz S. Segregation analysis of HL-A antigens and haplotypes in 50 families of patients with celiac disease. TransplantProc 1973; 5:1785-9.","journal-title":"TransplantProc"},{"key":"ref_7","first-page":"532","article-title":"HLA-DR phenotypes in Spanish coeliac children: their contribution to the understanding","volume-title":"of the genetics of the disease. Gut","volume":"24","author":"Mearin, M.L.; Biemond, I.; Pena, A.S.; Polanco, I.; Vasquez, C.","year":"1983","unstructured":"Mearin ML, Biemond I, Pena AS, Polanco I, Vasquez C. HLA-DR phenotypes in Spanish coeliac children: their contribution to the understanding of the genetics of the disease. Gut 1983; 24: 532-7."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1084\/jem.164.1.333","article-title":"An HLA-D region restriction fragment length polymorphism associated with celiac disease","volume":"164","author":"Howell, M.D.; Austin, R.K.; Kelleher, D.; Nepom, G.T.; Kagnoff, M.F.","year":"1986","unstructured":"Howell MD, Austin RK, Kelleher D, Nepom GT, Kagnoff MF. An HLA-D region restriction fragment length polymorphism associated with celiac disease. J Exp Med 1986; 164: 333-8.","journal-title":"J Exp Med"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1016\/S0140-6736(87)92544-X","article-title":"Susceptibility to coeliac disease involves genes in the HLA-DP subregion","volume":"ii:","author":"Niven, M.J.; Gaffrey, C.; Sachs, J.A.","year":"1987","unstructured":"Niven MJ, Gaffrey C, Sachs JA, et al. Susceptibility to coeliac disease involves genes in the HLA-DP subregion. Lancet 1987; ii: 805.","journal-title":"Lancet"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1136\/pgmj.58.686.797","article-title":"Coeliac disease in monozygotic twins","volume":"58","author":"Shale, D.J.; Johnston, D.G.; Hall, R.; Robert, D.F.","year":"1982","unstructured":"Shale DJ, Johnston DG, Hall R, Robert DF. Coeliac disease in monozygotic twins. Postgrad MedJ 1982; 58: 797-8.","journal-title":"Postgrad MedJ"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1007\/978-94-011-8114-3_27","article-title":"Gluten-sensitive enteropathy in Spain: genetic and environmental factors","volume-title":"The genetics ofcoeliac disease","author":"Polanco, I.; Biemond, I.; Van Leeuwen, A.","year":"1981","unstructured":"Polanco I, Biemond I, Van Leeuwen A, et al. Gluten-sensitive enteropathy in Spain: genetic and environmental factors. In: McConnell RB, ed. The genetics ofcoeliac disease. Lancaster: MTP Press, 1981: 211-31."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1016\/0016-5085(87)90017-5","article-title":"Effect of gluten supplementation in healthy siblings of children with celiac disease","volume":"92","author":"Polanco, I.; Mearin, M.L.; Larrauri, J.; Biemond, I.; Wipink-Baker, A.; Pena, A.S.","year":"1987","unstructured":"Polanco I, Mearin ML, Larrauri J, Biemond I, Wipink-Baker A, Pena AS. Effect of gluten supplementation in healthy siblings of children with celiac disease. Gastroenterology 1987; 92:678-81.","journal-title":"Gastroenterology"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1544","DOI":"10.1084\/jem.160.5.1544","article-title":"Possible role for a human adenovirus in the pathogenesis of celiac disease","volume":"160","author":"Kagnoff, M.F.; Austin, R.K.; Hubert, J.J.; Kasarda, D.D.","year":"1984","unstructured":"Kagnoff MF, Austin RK, Hubert JJ, Kasarda DD. 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Celiac sprue: correlation with murine T-cell responses to wheat gliadin components. J Immunol 1982; 129: 2693-7.","journal-title":"J Immunol"},{"key":"ref_16","first-page":"41","article-title":"In vitro (organ culture) studies of the toxicity of specific A-gliadin peptides","volume-title":"celiac disease. Gastroenterology 1988; 94","author":"G, De Ritis; S, Auricchio; HW, Jones; EJ-L, Lew; JE, Bernardin; DD, Kasarda","unstructured":"De Ritis G, Auricchio S, Jones HW, Lew EJ-L, Bernardin JE, Kasarda DD. In vitro (organ culture) studies of the toxicity of specific A-gliadin peptides in celiac disease. Gastroenterology 1988; 94: 41-9."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1136\/gut.28.8.995","article-title":"Evidence for the role of a human intestinal adenovirus in the pathogenesis of coeliac disease","volume":"28","author":"Kagnoff, M.F.; Paterson, Y.J.; Kumar, P.J.","year":"1987","unstructured":"Kagnoff MF, Paterson YJ, Kumar PJ, et al. Evidence for the role of a human intestinal adenovirus in the pathogenesis of coeliac disease. Gut 1987; 28: 995-1001.","journal-title":"Gut"},{"key":"ref_18","series-title":"Gut","first-page":"1469","article-title":"Evidence against the involvement of adenovirus 12 in the pathogenesis of coeliac disease","volume":"29","author":"Howdle, P.D.; Blair Zaidel, M.E.; Smart, C.J.; Treidosiewicz, L.K.; Blair, G.E.; Losowsky, M.S.","year":"1988","unstructured":"Howdle PD, Blair Zaidel ME, Smart CJ, Treidosiewicz LK, Blair GE, Losowsky MS. Evidence against the involvement of adenovirus 12 in the pathogenesis of coeliac disease [Abstract]. Gut 1988; 29: A1469."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"884","DOI":"10.1016\/S0140-6736(87)92859-5","article-title":"Cell-mediated immunity to a synthetic gliadin peptide resembling a sequence from adenovirus 12","volume":"i:","author":"Karagiannis, J.A.; Priddle, J.D.; Jewell, D.P.","year":"1987","unstructured":"Karagiannis JA, Priddle JD, Jewell DP. Cell-mediated immunity to a synthetic gliadin peptide resembling a sequence from adenovirus 12. Lancet 1987; i: 884-6.","journal-title":"Lancet"},{"key":"ref_20","first-page":"427","article-title":"Cellular hypersensitivity in celiac disease. Immune responses of sensitized lymphocytes to a synthetic dodecapeptide sequence from A-gliadin","volume":"1","author":"Karagiannis, J.A.; Jewell, D.P.","year":"1988","unstructured":"Karagiannis JA, Jewell DP. Cellular hypersensitivity in celiac disease. Immune responses of sensitized lymphocytes to a synthetic dodecapeptide sequence from A-gliadin [Abstract]. Gastroenterol Int 1988; suppl 1: 427."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1093\/ajcp\/69.2.103","volume":"69","author":"Terasaki, P.I.; Bernoco, D.; Parks, M.S.","year":"1978","unstructured":"Terasaki PI, Bernoco D, Parks MS, et al. Microdroplet testing for HLA-A,B,C and DR antigens. Am J Pathol 1978; 69: 103-20.","journal-title":"Am J Pathol"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"546","DOI":"10.4049\/jimmunol.110.2.546","article-title":"Migration inhibitory effect of cell-free supematants from tuberculin-stimulated cultures of human mononuclear leucocytes demonstrated by two-step MIF agarose assay","volume":"110","author":"Clausen, J.E.","year":"1973","unstructured":"Clausen JE. Migration inhibitory effect of cell-free supematants from tuberculin-stimulated cultures of human mononuclear leucocytes demonstrated by two-step MIF agarose assay. J Immunol 1973; 110: 546-5 1.","journal-title":"J Immunol"},{"key":"ref_23","first-page":"295","article-title":"In: Practical non-parametric statistics","author":"Conover, W.J.","year":"1980","unstructured":"Conover WJ. In: Practical non-parametric statistics. 2nd ed. New York: John Wiley, 1980: 295-9.","edition":"2nd ed"},{"key":"ref_24","first-page":"383","article-title":"Structure and genomic organization of adenoviruses","volume-title":"tumor viruses","author":"Flint, S.J.","year":"1980","unstructured":"Flint SJ. Structure and genomic organization of adenoviruses. In: Tooze J, ed. DNA tumor viruses. New York: Cold Spring Harbor Laboratories, 1980: 383-441."},{"key":"ref_25","first-page":"211","article-title":"Role of viruses in pediatric gastrointestinal disease and epidemiologic factors","volume-title":"infections ofthe gastrointestinal tract","author":"Middleton, P.J.","year":"1982","unstructured":"Middleton PJ. Role of viruses in pediatric gastrointestinal disease and epidemiologic factors. In: Tyrrell DAJ, Kapikian AZ, eds. Virus infections ofthe gastrointestinal tract. New York: Dekker, 1982: 211-25."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1136\/gut.19.2.126","article-title":"Cell-mediated immunity to gluten fraction III in adult coeliac disease","volume":"19","author":"Bullen, A.W.; Losowsky, M.S.","year":"1978","unstructured":"Bullen AW, Losowsky MS. Cell-mediated immunity to gluten fraction III in adult coeliac disease. Gut 1978; 19: 126-31.","journal-title":"Gut"},{"key":"ref_27","first-page":"83","article-title":"Cellular response to alpha-gliadin in untreated coeliac disease","volume":"23","author":"O'Farrelly, C.; Feighery, C.F.; Greally, J.F.; Weir, D.G.","year":"1984","unstructured":"O'Farrelly C, Feighery CF, Greally JF, Weir DG. Cellular response to alpha-gliadin in untreated coeliac disease. Gut 1984; 23: 83-7.","journal-title":"Gut"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1042\/cs072089Pa","article-title":"Preliminary characterization of leucocyte migration inhibition factor (LIF) produced by lymphocytes from coeliac patients when stimulated with gluten peptides","volume":"72","author":"Lydford-Davis, H.; Karagiannis, J.A.; Priddle, J.D.; Jewell, D.P.","year":"1987","unstructured":"Lydford-Davis H, Karagiannis JA, Priddle JD, Jewell DP. Preliminary characterization of leucocyte migration inhibition factor (LIF) produced by lymphocytes from coeliac patients when stimulated with gluten peptides. Clin Sci 1987; 72: 89P.","journal-title":"Clin Sci"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1136\/gut.22.8.633","article-title":"HLA-B8 and cell-mediated immunity to gluten","volume":"22","author":"Simpson, F.G.; Bullen, A.W.; Robertson, D.A.F.; Losowsky, M.S.","year":"1981","unstructured":"Simpson FG, Bullen AW, Robertson DAF, Losowsky MS. HLA-B8 and cell-mediated immunity to gluten. Gut 1981; 22: 633-6.","journal-title":"Gut"},{"key":"ref_30","first-page":"259","article-title":"Cell-mediated immunity to gluten fraction III in adult coeliac disease","volume":"24","author":"Holmes, G.K.T.; Asquith, P.; Cooke, W.T.","year":"1976","unstructured":"Holmes GKT, Asquith P, Cooke WT. Cell-mediated immunity to gluten fraction III in adult coeliac disease. Clin Exp Immunol 1976; 24: 259-65.","journal-title":"Clin Exp Immunol"},{"key":"ref_31","first-page":"389","article-title":"Stimulation of lymphocytes from patients with coeliac disease by a subfraction of gluten","volume":"ii:","author":"Sikora, K.; Anand, B.S.; Truelove, S.C.; Ciclitira, P.J.; Offord, R.E.","year":"1976","unstructured":"Sikora K, Anand BS, Truelove SC, Ciclitira PJ, Offord RE. Stimulation of lymphocytes from patients with coeliac disease by a subfraction of gluten. Lancet 1976; ii: 389-91.","journal-title":"Lancet"},{"key":"ref_32","first-page":"1305","article-title":"Suppressor-cell activity in coeliac disease induced by alphagliadin, a dietary antigen","volume":"ii:","author":"O'Farrelly, C.; Whelan, C.A.; Feighery, C.F.; Weir, D.G.","year":"1984","unstructured":"O'Farrelly C, Whelan CA, Feighery CF, Weir DG. Suppressor-cell activity in coeliac disease induced by alphagliadin, a dietary antigen. Lancet 1984; ii: 1305-7.","journal-title":"Lancet"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1136\/gut.28.4.426","article-title":"Cellular hypersensitivity to gluten derived peptides in coeliac disease","volume":"28","author":"Guan, R.; Rawcliffe, P.M.; Priddle, J.D.; Jewell, D.P.","year":"1987","unstructured":"Guan R, Rawcliffe PM, Priddle JD, Jewell DP. Cellular hypersensitivity to gluten derived peptides in coeliac disease. 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