{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T17:50:59Z","timestamp":1775065859739,"version":"3.50.1"},"reference-count":33,"publisher":"BMJ","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"published-print":{"date-parts":[[1991,3]]},"abstract":"<jats:p>Blood group antigen expression in the distal human colon is related to the development of the organ and is modified by malignant transformation. To elucidate the biochemical basis for these changes, we have (a) analysed the activity of glycosyltransferases coded for by the H, Se, Le, X, and A genes, in tissue biopsy specimens from normal and malignant proximal and distal human colon; (b) characterised the glycosphingolipids expressed in the various regions of normal and malignant colon by immunostaining of high performance thin layer chromatography plates; and (c) located the antigens on tissue sections from the same subjects by immunohistochemistry. In both secretors and non-secretors we found a significantly higher activity of alpha-2-L-fucosyltransferases in carcinomatous rectal tissue than in tissue from normal subjects, whereas the other transferase activities studied showed no significant differences. The acceptor substrate specificity suggested that both the Se and the H gene dependent alpha-2-L-fucosyltransferases are increased in carcinomas. In non-malignant tissue the only enzyme which showed appreciably higher activity in caecum than in rectum was alpha-2-L-fucosyltransferase. Immunochemistry and immunohistochemistry showed alpha-2-L-fucosylated structures in normal caecum from secretors and in tumour tissue from both secretors and non-secretors. We conclude that the alpha-2-L-fucosyltransferases control the expression of ABH, and Lewis(b) structures in normal and malignant colon.<\/jats:p>","DOI":"10.1136\/gut.32.3.287","type":"journal-article","created":{"date-parts":[[2007,9,18]],"date-time":"2007-09-18T17:41:31Z","timestamp":1190137291000},"page":"287-293","source":"Crossref","is-referenced-by-count":61,"title":["Regulation of the oncodevelopmental expression of type 1 chain ABH and Lewis(b) blood group antigens in human colon by alpha-2-L-fucosylation."],"prefix":"10.1136","volume":"32","author":[{"given":"T F","family":"Orntoft","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P","family":"Greenwell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H","family":"Clausen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W M","family":"Watkins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1991,3,1]]},"reference":[{"key":"ref_1","first-page":"161","article-title":"and related antigens","volume":"51","author":"Oriol, R.; Le Pendu, J.; of ABO, Mollicone R.Genetics; H, Lewis; X.","year":"1986","unstructured":"Oriol R, Le Pendu J, Mollicone R. Genetics of ABO, H, Lewis, X, and related antigens. Vox Sang 1986; 51: 161-71.","journal-title":"Vox Sang"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1111\/1523-1747.ep12258874","article-title":"Pattern of distribution of blood group antigens on human epidermal cells during maturation","volume":"82","author":"Dabelsteen, E.; Buschard, K.; Hakomori, S.; Young, W.W.","year":"1984","unstructured":"Dabelsteen E, Buschard K, Hakomori S, Young WW. Pattern of distribution of blood group antigens on human epidermal cells during maturation.J Invest Dermatol 1984; 82: 13-7.","journal-title":"J Invest Dermatol"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1111\/1523-1747.ep12510411","article-title":"Carbohydrate chains specific for blood group antigens in differentiation of human oral epithelium","volume":"79","author":"Dabelsteen, E.; Vedtofte, P.; Hakomori, S.; Young, W.W.","year":"1982","unstructured":"Dabelsteen E, Vedtofte P, Hakomori S, Young WW. Carbohydrate chains specific for blood group antigens in differentiation of human oral epithelium. J Invest Dermatol 1982; 79: 3-7.","journal-title":"J Invest Dermatol"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1038\/314053a0","article-title":"Demonstration by monoclonal antibodies that carbohydrate structures ofglycoproteins and glycolipids are oncodevelopmental antigens","volume":"314","author":"Feizi, T.","year":"1985","unstructured":"Feizi T. Demonstration by monoclonal antibodies that carbohydrate structures ofglycoproteins and glycolipids are oncodevelopmental antigens. Nature 1985; 314: 53-7.","journal-title":"Nature"},{"key":"ref_5","first-page":"11331","article-title":"Oncofetal expression of Le, carbohydrate antigens in human colonic adenocarcinomas","volume":"262","author":"Holmes, E.H.; Ostrander, G.K.; Clausen, H.; Graem, N.","year":"1987","unstructured":"Holmes EH, Ostrander GK, Clausen H, Graem N. Oncofetal expression of Le, carbohydrate antigens in human colonic adenocarcinomas..BiolChem 1987; 262: 11331-8.","journal-title":"BiolChem"},{"key":"ref_6","first-page":"4499","article-title":"Distribution of blood group antigens","volume":"45","author":"Yuan, M.; Itzkowitz, S.H.; Palekar, A.","year":"1985","unstructured":"Yuan M, Itzkowitz SH, Palekar A, et al. Distribution of blood group antigens A, B, H, Lewis,, and Lewisb in human normal, fetal, and malignant colonic tissue. CancerRes 1985; 45: 4499-511.","journal-title":"CancerRes; Lewisb in human normal, fetal, and malignant colonic tissue"},{"key":"ref_7","first-page":"2627","article-title":"Lewisland sialylated Lewis'-related antigen expression in human malignant and non-malignant colonic tissues","volume":"46","author":"Itzkowitz, S.H.; Yuan, M.; Fukushi, Y.","year":"1986","unstructured":"Itzkowitz SH, Yuan M, Fukushi Y, et al. Lewisland sialylated Lewis'-related antigen expression in human malignant and non-malignant colonic tissues. Cancer Res 1986; 46:2627-32.","journal-title":"Cancer Res"},{"key":"ref_8","first-page":"5985","article-title":"Expression of Ley and extended Ley blood group-related antigens in human malignant, premalignant, and non-malignant colonic tissues","volume":"46","author":"Kim, Y.S.; Yuan, M.; Itzkowitz, S.H.","year":"1986","unstructured":"Kim YS, Yuan M, Itzkowitz SH, et al. Expression of Ley and extended Ley blood group-related antigens in human malignant, premalignant, and non-malignant colonic tissues. CancerRes 1986; 46: 5985-92.","journal-title":"CancerRes"},{"key":"ref_9","first-page":"503","article-title":"The histological distribution of the blood group substances in man as disclosed by immunofluorescence III. The","volume":"119","author":"Szulman, A.E.","year":"1984","unstructured":"Szulman AE. The histological distribution of the blood group substances in man as disclosed by immunofluorescence III. The A, B, and H antigens in embryos and fetuses from 18 mm length. J Exp Med 1984; 119: 503-15.","journal-title":"J Exp Med; antigens in embryos and fetuses from 18 mm length"},{"key":"ref_10","first-page":"565","article-title":"The histological distribution of the blood group substances in man as disclosed by immunofluorescence VI. The Le, and Leb antigens during fetal development","volume":"5","author":"Szulman, A.E.; Marcus, D.M.","year":"1973","unstructured":"Szulman AE, Marcus DM. The histological distribution of the blood group substances in man as disclosed by immunofluorescence VI. The Le, and Leb antigens during fetal development. Lab Invest 1973; 5: 565-73.","journal-title":"Lab Invest"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"11254","DOI":"10.1016\/S0021-9258(18)67376-4","article-title":"Novel fucolipids of human adenocarcinoma VI. Monoclonal antibody specific for trifucosyl Ley (III3Fuc V'Fuc VI'Fucn LC6), which does not crossreact with Ley antigen, and a possible conformational epitope structure","volume":"261","author":"Kaizu, T.; Levery, S.B.; Nudelman, E.; Kamp, R.E.S.; Hakomori, S.","year":"1986","unstructured":"Kaizu T, Levery SB, Nudelman E, Kamp RES, Hakomori S. Novel fucolipids of human adenocarcinoma VI. Monoclonal antibody specific for trifucosyl Ley (III3Fuc V'Fuc VI'Fucn LC6), which does not crossreact with Ley antigen, and a possible conformational epitope structure. J Biol Chem 1986;261: 11254-8.","journal-title":"J Biol Chem"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"4681","DOI":"10.1016\/S0021-9258(17)43100-0","article-title":"Novel fucolipids accumulating in human adenocarcinoma: II. Selective isolation ofhybridoma antibodies that differentially recognise mono-, di-, and trifucosylated type 2 chain","volume":"259","author":"Fukushi, Y.; Hakomori, S.; Nudelman, E.; Cochran, N.","year":"1984","unstructured":"Fukushi Y, Hakomori S, Nudelman E, Cochran N. Novel fucolipids accumulating in human adenocarcinoma: II. Selective isolation ofhybridoma antibodies that differentially recognise mono-, di-, and trifucosylated type 2 chain. J Biol Chem 1984; 259: 4681-5.","journal-title":"J Biol Chem"},{"key":"ref_13","first-page":"14365","article-title":"A monoclonal antibody-defined antigen associated with gastro-intestinal cancer is a ganglioside containing sialylated lacto-Nfucopentaose","volume":"257","author":"Magnani, J.L.; Nilsson, B.; Brockhaus, M.","year":"1982","unstructured":"Magnani JL, Nilsson B, Brockhaus M, et al. A monoclonal antibody-defined antigen associated with gastro-intestinal cancer is a ganglioside containing sialylated lacto-Nfucopentaose II.JrBiol Chem 1982; 257: 14365-9.","journal-title":"II. JrBiol Chem"},{"key":"ref_14","first-page":"2619","article-title":"Quantitative and qualitative characterization of human cancer-associated serum glycoprotein antigens expressing fucosyl or sialylfucosyl type 2 chain polylactosamine","volume":"46","author":"Kannagi, R.; Fukushi, Y.; Tachikwa, T.","year":"1986","unstructured":"Kannagi R, Fukushi Y, Tachikwa T, et al. Quantitative and qualitative characterization of human cancer-associated serum glycoprotein antigens expressing fucosyl or sialylfucosyl type 2 chain polylactosamine. Cancer Res 1986; 46: 2619-26.","journal-title":"Cancer Res"},{"key":"ref_15","first-page":"5279","article-title":"Characterisation of sialosylated Lewis' as a new tumour-associated antigen","volume":"44","author":"Fukushima, K.; Hirota, M.; Terasaki, P.I.","year":"1984","unstructured":"Fukushima K, Hirota M, Terasaki PI, et al. Characterisation of sialosylated Lewis' as a new tumour-associated antigen. Cancer Res 1984; 44: 5279-85.","journal-title":"Cancer Res"},{"key":"ref_16","first-page":"141","article-title":"Monoclonal antibody detection of a circulating tumorassociated antigen II. A longitudinal evaluation of patients with colorectal cancer","volume":"2","author":"Sears, H.F.; Herlyn, M.; DelVillano, B.; Steplewski, Z.; Koprowski, H.","year":"1984","unstructured":"Sears HF, Herlyn M, DelVillano B, Steplewski Z, Koprowski H. Monoclonal antibody detection of a circulating tumorassociated antigen II. A longitudinal evaluation of patients with colorectal cancer. J Clin Immunol 1984; 2: 141-9.","journal-title":"J Clin Immunol"},{"key":"ref_17","first-page":"11793","article-title":"The monoclonal antibody directed to difucosylated type 2","volume":"258","author":"Abe, K.; McKibbin, J.M.; Hakomori, S.","year":"1983","unstructured":"Abe K, McKibbin JM, Hakomori S. The monoclonal antibody directed to difucosylated type 2 chain (Fucal-2Galfll-4 [Fucal-3]GlcNAc; Y determinant). J Biol Chem 1983; 258: 11793-7.","journal-title":"J Biol Chem"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1951","DOI":"10.4049\/jimmunol.132.4.1951","article-title":"The antibody specific to type 1 chain blood group A determinant","volume":"132","author":"Abe, K.; Levery, S.B.; Hakomori, S.","year":"1984","unstructured":"Abe K, Levery SB, Hakomori S. The antibody specific to type 1 chain blood group A determinant. J Immunol 1984; 132: 1951-4.","journal-title":"J Immunol"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"3578","DOI":"10.1021\/bi00335a028","article-title":"Blood group A determinants with monoand difucosyl type 1 chain in human erythrocyte membranes","volume":"24","author":"Clausen, J.; Levery, S.B.; McKibbin, J.M.; Hakomori, S.","year":"1985","unstructured":"Clausen J, Levery SB, McKibbin JM, Hakomori S. Blood group A determinants with monoand difucosyl type 1 chain in human erythrocyte membranes. Biochemistry 1985; 24: 3578-86.","journal-title":"Biochemistry"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"6190","DOI":"10.1021\/bi00343a024","article-title":"Monoclonal antibodies defining blood group A variants with difucosyl type 1 chain (ALeb) and difucosyl type 2 chain (ALey)","volume":"24","author":"Clausen, H.; McKibbin, J.M.; Hakomori, S.","year":"1985","unstructured":"Clausen H, McKibbin JM, Hakomori S. Monoclonal antibodies defining blood group A variants with difucosyl type 1 chain (ALeb) and difucosyl type 2 chain (ALey). Biochemistry 1985; 24: 6190-4.","journal-title":"Biochemistry"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"7075","DOI":"10.1021\/bi00370a048","article-title":"Further characterization of type 2 and type 3 chain blood group A glycosphingolipids from human erythrocyte membranes","volume":"25","author":"Clausen, H.; Levery, S.B.; Nudelman, E.; Baldwin, M.; Hakomori, S.","year":"1986","unstructured":"Clausen H, Levery SB, Nudelman E, Baldwin M, Hakomori S. Further characterization of type 2 and type 3 chain blood group A glycosphingolipids from human erythrocyte membranes. Biochemistry 1986; 25: 7075-85.","journal-title":"Biochemistry"},{"issue":"0","key":"ref_22","first-page":"4427","article-title":"Variant forms of a-2-L-fucosyltransferase in human submaxillary glands from blood group ABH \"secretor\" and \"non-secretor\" individuals. Glycoconjugatej 1985; 2","volume":"23","author":"Betteridge, A.; Watkins, W.M.","year":"1988","unstructured":"Betteridge A, Watkins WM. Variant forms of a-2-L-fucosyltransferase in human submaxillary glands from blood group ABH \"secretor\" and \"non-secretor\" individuals. Glycoconjugatej 1985; 2: 61-78. 23 0rntoft TF, Wolf H, Watkins WM. Activity of the human blood group ABO, Se, H, Le, and X gene-encoded glycosyltransferases in normal and malignant bladder urothelium. CancerRes 1988; 48: 4427-33.","journal-title":"CancerRes; Activity of the human blood group ABO"},{"key":"ref_23","first-page":"1","article-title":"Carbohydrate antigens in higher animals","volume-title":"Handbook of experimental immunology. Vol 4","author":"Hakomori, S.; Kannagi, R.","year":"1986","unstructured":"Hakomori S, Kannagi R. Carbohydrate antigens in higher animals. In: Weir DM, Herzenberg LA, Blackwell CC, eds. Handbook of experimental immunology. Vol 4. Oxford: Blackwell Scientific Publications 1986: 1-39."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/S0022-2275(20)39537-7","article-title":"Quantitative isolation of total glycosphingolipids from animal cells","volume":"12","author":"Saito, T.; S, Hakomori","year":"1971","unstructured":"Saito, T, Hakomori S. Quantitative isolation of total glycosphingolipids from animal cells. I Lipid Res 1971; 12: 257-62.","journal-title":"I Lipid Res"},{"key":"ref_25","first-page":"680","article-title":"Gangliosides of human, bovine, and rabbit plasma","volume":"13","author":"Yu, R.K.; Ledeen, R.W.","year":"1972","unstructured":"Yu RK, Ledeen RW. Gangliosides of human, bovine, and rabbit plasma.JLipidRes 1972; 13: 680-6.","journal-title":"JLipidRes"},{"key":"ref_26","first-page":"399402","article-title":"Detection ofgangliosides that bind cholera toxin: direct binding of 'II-labelled toxin to thin-layer chromatograms","volume":"109","author":"Magnani, J.L.; Smith, D.F.; Ginsburg, V.","year":"1980","unstructured":"Magnani JL, Smith DF, Ginsburg V. Detection ofgangliosides that bind cholera toxin: direct binding of 'II-labelled toxin to thin-layer chromatograms. Anal Biochem 1980; 109: 399402.","journal-title":"Anal Biochem"},{"key":"ref_27","first-page":"220","article-title":"Qualitative difference in the N-acetyl-Dgalactosaminyltransferases produced by human A'","volume-title":"and A' genes. Proc Natl Acad Sci","volume":"70","author":"Schachter, H.; Michaels, M.A.; Tilley, C.A.; Crookston, M.C.; Crookston, J.","year":"1973","unstructured":"Schachter H, Michaels MA, Tilley CA, Crookston MC, Crookston J. Qualitative difference in the N-acetyl-Dgalactosaminyltransferases produced by human A' and A' genes. Proc Natl Acad Sci USA 1973; 70: 220-4."},{"key":"ref_28","series-title":"Proceedings of the Fifth International Symposium Kiel. [Abstract]","first-page":"639","article-title":"Altered biosynthesis of blood group related antigens in neoplastic colon mucosa","author":"Piller, F.; Schenkel-Brunner, H.; Tuppy, H.","year":"1979","unstructured":"Piller F, Schenkel-Brunner H, Tuppy H. Altered biosynthesis of blood group related antigens in neoplastic colon mucosa. In: Schauer R, Kramer MF, Vliegenhart JFG, Wiegandt H, eds. Glycoconjugates. Proceedings of the Fifth International Symposium Kiel. [Abstract] Stuttgart. Georg Thieme, 1979: 639-40."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"4193","DOI":"10.1073\/pnas.81.13.4193","article-title":"Biochemical evidence that secretor gene, Se, is a structural gene encoding a specific fucosyltransferase","volume":"81","author":"Kumazaki, T.; Yoshida, A.","year":"1984","unstructured":"Kumazaki T, Yoshida A. Biochemical evidence that secretor gene, Se, is a structural gene encoding a specific fucosyltransferase. Proc Natl Acad Sci USA 1984; 81: 4193-7.","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref_30","first-page":"4415","article-title":"Biosynthesis of blood group ABH antigens: genetic regulation and tissue distribution","volume-title":"of glycosyltransferases. Transplant Proc","volume":"19","author":"Watkins, W.M.; Greenwell, P.","year":"1987","unstructured":"Watkins WM, Greenwell P. Biosynthesis of blood group ABH antigens: genetic regulation and tissue distribution of glycosyltransferases. Transplant Proc 1987; 19: 4415-6."},{"key":"ref_31","first-page":"3605","article-title":"a-2-L-Fucosyltransferase activity in sera ofindividuals with H-deficient red cells and normal H antigen in secretions","volume":"44","author":"J, Le Pendu; R, Oriol; G, Juszczak","year":"1983","unstructured":"Le Pendu J, Oriol R, Juszczak G, et al. a-2-L-Fucosyltransferase activity in sera ofindividuals with H-deficient red cells and normal H antigen in secretions. Vox Sang 1983; 44: 3605.","journal-title":"Vox Sang"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1111\/j.1432-1033.1976.tb10944.x","article-title":"Phenyl P-D-galactopyranoside as an acceptor substrate for the blood-group H gene-associated guanosine diphosphate L-fucose: [3-Dgalactosyl a-2-L-fucosyltransferase","volume":"69","author":"Chester, M.A.; Yates, A.D.; Watkins, W.M.","year":"1976","unstructured":"Chester MA, Yates AD, Watkins WM. Phenyl P-D-galactopyranoside as an acceptor substrate for the blood-group H gene-associated guanosine diphosphate L-fucose: [3-Dgalactosyl a-2-L-fucosyltransferase. Eur J Biochem 1976; 69: 583-92.","journal-title":"Eur J Biochem"},{"key":"ref_33","first-page":"43945","article-title":"Enzymatic conversion of H, glycolipid and deficiency of these enzyme activities in adenocarcinoma","volume":"55","author":"Stellner, K.; Hakomori, S.; Wagner, G.A.","year":"1973","unstructured":"Stellner K, Hakomori S, Wagner GA. Enzymatic conversion of H, glycolipid and deficiency of these enzyme activities in adenocarcinoma. Biochem Biophys Res Commun 1973; 55: 43945.","journal-title":"Biochem Biophys Res Commun"}],"container-title":["Gut"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/gut.32.3.287","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,7]],"date-time":"2025-09-07T18:46:13Z","timestamp":1757270773000},"score":1,"resource":{"primary":{"URL":"https:\/\/gut.bmj.com\/lookup\/doi\/10.1136\/gut.32.3.287"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,3]]},"references-count":33,"journal-issue":{"issue":"3","published-online":{"date-parts":[[1991,3,1]]},"published-print":{"date-parts":[[1991,3]]}},"alternative-id":["10.1136\/gut.32.3.287"],"URL":"https:\/\/doi.org\/10.1136\/gut.32.3.287","relation":{},"ISSN":["0017-5749","1468-3288"],"issn-type":[{"value":"0017-5749","type":"print"},{"value":"1468-3288","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,3]]}}}