{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T19:24:39Z","timestamp":1763753079434,"version":"3.45.0"},"reference-count":25,"publisher":"BMJ","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"published-print":{"date-parts":[[1992,11]]},"abstract":"<jats:p>A dodecapeptide of A-gliadin, which shares amino acid homologies with the E1b protein of adenovirus 12, was used to produce murine monoclonal antibodies. Five monoclonal antibodies were produced and were screened by enzyme linked immunosorbant assay, immunodot assay, and immunoblotting. The antibodies were tested against whole wheat gliadin and its alpha, beta, gamma, and omega subfractions, and the prolamins of rye, barley, oats, maize, millet, rice, and sorghum. Four of the five antibodies cross reacted with one or more of the coeliac non-toxic cereals--maize, millet, sorghum, and rice. The monoclonal antibody that did not cross react with these non-toxic cereals, did not recognize Frazer's fraction III, a peptic-tryptic digest of wheat gluten which is known to be toxic. The results suggest that the A-gliadin dodecapeptide shares a region of homology with cereals that do not exacerbate coeliac disease. This study does not support the hypothesis that prior infection with adenovirus 12 is a precipitating factor in coeliac disease.<\/jats:p>","DOI":"10.1136\/gut.33.11.1504","type":"journal-article","created":{"date-parts":[[2007,9,18]],"date-time":"2007-09-18T17:41:31Z","timestamp":1190137291000},"page":"1504-1507","source":"Crossref","is-referenced-by-count":17,"title":["Coeliac disease: characterisation of monoclonal antibodies raised against a synthetic peptide corresponding to amino acid residues 206-217 of A-gliadin."],"prefix":"10.1136","volume":"33","author":[{"given":"H J","family":"Ellis","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A P","family":"Doyle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R P","family":"Sturgess","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P J","family":"Ciclitira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1992,11,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1111\/j.1651-2227.1953.tb05586.x","article-title":"Coeliac disease IV. An investigation of the injurious constituents of wheat in connection with their action on patients with coeliac disease","volume":"42","author":"JHvd, Kamer; HA, Weijers; WK, Dicke","year":"1953","unstructured":"Kamer JHvd, Weijers HA, Dicke WK. Coeliac disease IV. An investigation of the injurious constituents of wheat in connection with their action on patients with coeliac disease. Acta Paediatr 1953; 42: 223-31.","journal-title":"Acta Paediatr"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1080\/00365521.1984.12005681","article-title":"Are all gliadins toxic in coeliac disease? An in vitro study of a,, B, y and w gliadins","volume":"19","author":"Howdle, P.D.; Ciclitra, P.J.; Simpson, F.G.; Losowsky, M.S.","year":"1984","unstructured":"Howdle PD, Ciclitra PJ, Simpson FG, Losowsky MS. Are all gliadins toxic in coeliac disease? An in vitro study of a,,B, y and w gliadins. ScandJ Gastroenterol 1984; 19: 41-7.","journal-title":"ScandJ Gastroenterol"},{"key":"ref_3","first-page":"4712","article-title":"Nucleic acid (cDNA) and amino acid sequences of a type gliadins from","volume-title":"wheat (Triticum aestivum). Proc Natl Acad Sci","volume":"81","author":"Kasarda, D.D.; Okita, T.W.; Bernadin, J.E.; Backer, P.A.; Nimmo, C.; Lew, E.; etal","year":"1984","unstructured":"Kasarda DD, Okita TW, Bernadin JE, Backer PA, Nimmo C, Lew E, etal. Nucleic acid (cDNA) and amino acid sequences of a type gliadins from wheat (Triticum aestivum). Proc Natl Acad Sci 1984; 81: 4712-6."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1544","DOI":"10.1084\/jem.160.5.1544","article-title":"Possible role ofa human adenovirus in the pathogenesis of coeliac disease","volume":"160","author":"Kagnoff, M.R.; Raleigh, K.A.; Hubert, J.J.; Bernadin, J.F.; Kasarda, D.D.","year":"1984","unstructured":"Kagnoff MR, Raleigh KA, Hubert JJ, Bernadin JF, Kasarda DD. Possible role ofa human adenovirus in the pathogenesis of coeliac disease. J Exp Med 1984; 160: 1544-7.","journal-title":"J Exp Med"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1136\/gut.28.8.995","article-title":"Evidence for the role of a human intestinal adenovirus in the pathogenesis of coeliac disease","volume":"28","author":"Kagnoff, M.F.; Paterson, K.J.; Kumar, P.J.; Kasarda, D.D.; Carbone, F.R.; Unsworth, D.J.","year":"1987","unstructured":"Kagnoff MF, Paterson KJ, Kumar PJ, Kasarda DD, Carbone FR, Unsworth DJ, et al. Evidence for the role of a human intestinal adenovirus in the pathogenesis of coeliac disease. Gut 1987; 28: 995-1001.","journal-title":"Gut"},{"key":"ref_6","first-page":"282","article-title":"Lack of a serologic response to an E1B protein of adenovirus 12 in coeliac disease. Scand J3 Gastroenterol","volume":"24","author":"Howdle, P.D.; Blair Zadel, M.E.; Smart, C.J.; Trejdosiewicz, L.K.; Blair, G.E.; Losowsky, M.S.","year":"1989","unstructured":"Howdle PD, Blair Zadel ME, Smart CJ, Trejdosiewicz LK, Blair GE, Losowsky MS. Lack of a serologic response to an E1B protein of adenovirus 12 in coeliac disease. Scand J3 Gastroenterol 1989; 24: 282-6."},{"key":"ref_7","first-page":"255","article-title":"No humoral response to the E1B-54kD protein of adenovirus 12 in patients with coeliac disease","volume":"3","year":"1991","unstructured":"Dieleman LA, Pena AS, van Doornick JH, Mearin ML, van Duiin W,. Lamers CBHW. No humoral response to the E1B-54kD protein of adenovirus 12 in patients with coeliac disease. EurJ Gastroenterol Hepatol 1991; 3: 255-7.","journal-title":"EurJ Gastroenterol Hepatol"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1563","DOI":"10.1136\/gut.30.11.1563","article-title":"Is a persistant adenovirus infection involved in coeliac disease","volume":"30","author":"Carter, M.J.; Willcocks, M.M.; Mitchison, H.C.; Record, C.O.; Madeley, C.R.","year":"1989","unstructured":"Carter MJ, Willcocks MM, Mitchison HC, Record CO, Madeley CR. Is a persistant adenovirus infection involved in coeliac disease. Gut 1989; 30: 1563-7.","journal-title":"Gut"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1114","DOI":"10.1136\/gut.32.10.1114","article-title":"Is persistant adenovirus 12 infection involved in coeliac disease? A search for viral DNA using the polymerase chain reaction","volume":"32","author":"Mahon, J.; Blair, G.E.; Wood, G.M.; Scott, B.B.; Losowsky, M.S.; Howdle, P.D.","year":"1991","unstructured":"Mahon J, Blair GE, Wood GM, Scott BB, Losowsky MS, Howdle PD. Is persistant adenovirus 12 infection involved in coeliac disease? A search for viral DNA using the polymerase chain reaction. Gut 1991; 32: 1114-6.","journal-title":"Gut"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"884","DOI":"10.1016\/S0140-6736(87)92859-5","article-title":"Cell-mediated immunity to a synthetic gliadin peptide resembling a sequence from adenovirus 12","volume":"i:","author":"Karagiannis, J.A.; Priddle, J.D.; Jewell, D.P.","year":"1987","unstructured":"Karagiannis JA, Priddle JD, Jewell DP. Cell-mediated immunity to a synthetic gliadin peptide resembling a sequence from adenovirus 12. Lancet 1987; i: 884-6.","journal-title":"Lancet"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1229","DOI":"10.1002\/jsfa.2740241011","article-title":"Large scale preparation of gliadin proteins","volume":"24","author":"Patey, A.L.; Evans, D.J.","year":"1973","unstructured":"Patey AL, Evans DJ. Large scale preparation of gliadin proteins. _J Sci Food Agric 1973; 24: 1229-33.","journal-title":"_J Sci Food Agric"},{"key":"ref_12","first-page":"252","article-title":"Gluten induced enteropathy: the effect of partially digested gluten","volume":"ii:","author":"Frazer, A.C.; Fletcher, R.F.; Ross, C.A.C.; Shaw, B.; Sammons, H.G.; Scheider, R.","year":"1959","unstructured":"Frazer AC, Fletcher RF, Ross CAC, Shaw B, Sammons HG, Scheider R. Gluten induced enteropathy: the effect of partially digested gluten. Lancet 1959; ii: 252-5.","journal-title":"Lancet"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the folin phenol reagent","volume":"193","author":"Lowry, O.H.; Rosebrough, N.J.; Farr, A.L.; Randall, R.J.","year":"1951","unstructured":"Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J Biol Chem 1951; 193: 265-75.","journal-title":"J Biol Chem"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/0022-1759(89)90283-4","article-title":"The production and characterisation of monoclonal antibodies to wheat gliadin peptides","volume":"120","author":"Ellis, H.J.; Freedman, A.R.; Ciclitira, P.J.","year":"1989","unstructured":"Ellis HJ, Freedman AR, Ciclitira PJ. The production and characterisation of monoclonal antibodies to wheat gliadin peptides.J Immunol Methods 1989; 120: 17-22.","journal-title":"J Immunol Methods"},{"key":"ref_15","first-page":"1548","article-title":"A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody secreting hybrid cell lines","volume-title":"I Immunol","volume":"123","author":"Kearney, J.F.; Radbruch, A.; Liesegang, B.; Rajewsky, K.","year":"1979","unstructured":"Kearney JF, Radbruch A, Liesegang B, Rajewsky K. A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody secreting hybrid cell lines. I Immunol 1979; 123: 1548-50."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/0076-6879(81)73054-4","article-title":"Preparation of monoclonal antibodies. Strategies and procedures","volume":"73","author":"Galfre, G.; Milstein, C.","year":"1981","unstructured":"Galfre G, Milstein C. Preparation of monoclonal antibodies. Strategies and procedures. Methods Enzymol 1981; 73: 3-46.","journal-title":"Methods Enzymol"},{"issue":"17","key":"ref_17","first-page":"15","article-title":"Two monoclonal antibodies to human small intestinal brush border membrane components","volume":"73","author":"Ellis, H.J.; Gal, E.; Nelufer, J.M.; Freedman, A.R.; Ciclitira, P.J.","year":"1987","unstructured":"Ellis HJ, Gal E, Nelufer JM, Freedman AR, Ciclitira PJ. Two monoclonal antibodies to human small intestinal brush border membrane components. Clin Sci 1987; 73 (suppl 17): 15.","journal-title":"Clin Sci"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/0009-8981(87)90436-0","article-title":"Detection of wheat gliadin contamination of gluten-free foods by monoclonal antibody dot immunobinding assay","volume":"166","author":"Freedman, A.R.; Galfre, G.; Gal, E.; Ellis, H.J.; Ciclitira, P.J.","year":"1987","unstructured":"Freedman AR, Galfre G, Gal E, Ellis HJ, Ciclitira PJ. Detection of wheat gliadin contamination of gluten-free foods by monoclonal antibody dot immunobinding assay. Clin Chim Acta 1987; 166: 323-8.","journal-title":"Clin Chim Acta"},{"key":"ref_19","first-page":"346","article-title":"Western immunoblotting of cereal proteins","volume":"85","author":"Freedman, A.R.; Galfre, G.; Gal, E.; Ellis, H.J.; Cicitira, P.J.","year":"1988","unstructured":"Freedman AR, Galfre G, Gal E, Ellis HJ, Cicitira PJ. Western immunoblotting of cereal proteins. Int Arch Allergy Appl Immunol 1988; 85: 346-50.","journal-title":"Int Arch Allergy Appl Immunol"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"987","DOI":"10.1002\/jsfa.2740361014","article-title":"A sensitive monoclonal-antibody-based test for gluten detection: quantitative immunoassay","volume":"36","author":"Skerritt, J.H.","year":"1985","unstructured":"Skerritt JH. A sensitive monoclonal-antibody-based test for gluten detection: quantitative immunoassay. J Sci Food Agric 1985; 36: 987-94.","journal-title":"J Sci Food Agric"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1042\/bj2700313","article-title":"Conformational studies of peptides corresponding to the coeliacactivating regions of wheat a-gliadin","volume":"270","year":"1990","unstructured":"Tatham AS, Marsh MN, Wieser H, Shewry PR. Conformational studies of peptides corresponding to the coeliacactivating regions of wheat a-gliadin. Biochem J 1990; 270: 313-8.","journal-title":"Biochem J"},{"key":"ref_22","first-page":"41","article-title":"In vitro (organ culture) studies of the toxicity of specific A-gliadin peptides","volume-title":"celiac disease. Gastroenterology 1988; 94","author":"de Ritis G; S, Auricchio; HW, Jones; EJ-L, Lew; JE, Bernardin; DD, Kasada","unstructured":"de Ritis G, Auricchio S, Jones HW, Lew EJ-L, Bernardin JE, Kasada DD. In vitro (organ culture) studies of the toxicity of specific A-gliadin peptides in celiac disease. Gastroenterology 1988; 94:41-9."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1136\/gut.31.6.668","article-title":"Cellular hypersensitivity to a synthetic dodecapeptide derived from human adenovirus 12 which resembles a sequence of A-gliadin in patients with coeliac disease","volume":"31","author":"Mantzaris, G.J.; Karagiannis, J.A.; Priddle, J.D.; Jewell, D.P.","year":"1990","unstructured":"Mantzaris GJ, Karagiannis JA, Priddle JD, Jewell DP. Cellular hypersensitivity to a synthetic dodecapeptide derived from human adenovirus 12 which resembles a sequence of A-gliadin in patients with coeliac disease. Gut 1990; 31:668-73.","journal-title":"Gut"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1159\/000235473","article-title":"Identification of reactive synthetic gliadin peptides specific for coeliac disease","volume":"95","author":"Devery, J.M.; Bender, V.; Penttila, I.; Skerritt, J.H.","year":"1991","unstructured":"Devery JM, Bender V, Penttila I, Skerritt JH. Identification of reactive synthetic gliadin peptides specific for coeliac disease. IntArch AllergyAppl Immunol 1991; 95: 356-62.","journal-title":"IntArch AllergyAppl Immunol"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"392","DOI":"10.3109\/00365529108996500","article-title":"In vivo toxicity of a synthetic dodecajpeptide from A-gliadin in patients with coeliac disease","volume":"26","author":"Mantzaris, G.; Jewell, D.P.","year":"1991","unstructured":"Mantzaris G, Jewell DP. In vivo toxicity of a synthetic dodecajpeptide from A-gliadin in patients with coeliac disease. ScandJ Gastroenterol 1991; 26: 392-8.","journal-title":"ScandJ Gastroenterol"}],"container-title":["Gut"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/gut.33.11.1504","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T19:19:46Z","timestamp":1763752786000},"score":1,"resource":{"primary":{"URL":"https:\/\/gut.bmj.com\/lookup\/doi\/10.1136\/gut.33.11.1504"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,11]]},"references-count":25,"journal-issue":{"issue":"11","published-online":{"date-parts":[[1992,11,1]]},"published-print":{"date-parts":[[1992,11]]}},"alternative-id":["10.1136\/gut.33.11.1504"],"URL":"https:\/\/doi.org\/10.1136\/gut.33.11.1504","relation":{},"ISSN":["0017-5749","1468-3288"],"issn-type":[{"type":"print","value":"0017-5749"},{"type":"electronic","value":"1468-3288"}],"subject":[],"published":{"date-parts":[[1992,11]]}}}