{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T16:56:11Z","timestamp":1765040171766,"version":"3.44.0"},"reference-count":28,"publisher":"BMJ","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"published-print":{"date-parts":[[1993,7]]},"abstract":"<jats:p>Injury of the oesophageal mucosa can result from exposure to refluxed gastric acid and pepsin. Competence of the lower oesophageal sphincter and peristaltic activity serve to reduce contract time between luminal acid and oesophageal mucosa, but intraluminal neutralisation of residual acid by bicarbonate may also be important in preventing oesophageal mucosal injury. Whereas swallowed saliva contains bicarbonate, recent experiments have also demonstrated alkali secretion from the mammalian oesophagus. Bicarbonate secretion from the human oesophagus was therefore examined with an intubation technique and perfusion of the oesophagus with a non-absorbable marker. Saliva, gastric, and oesophageal aspirates were collected and bicarbonate concentrations determined by measurements of pH and pCO2 or by back titration. In 32 normal subjects (17 women, 15 men) median basal oesophageal bicarbonate secretion determined by a pH\/pCO2 method was 416 (range 139-1050) mumol\/hour\/10 cm. In a subgroup of 15 experiments median oesophageal bicarbonate output was 489 (range 157-1033) mumol\/hour\/10 cm (pH\/pCO2 method) compared with a median alkali output of 563 (range 135-799) mumol\/hour\/10 cm as determined by back titration. The difference was not significant. Salivary contamination of the oesophagus accounted for 25% of all bicarbonate measured within the oesophagus and refluxed gastric bicarbonate accounted for 2.5%. Bicarbonate secretion from the normal human oesophagus may, in combination with swallowed salivary bicarbonate, play a part in preventing oesophageal mucosal damage due to refluxed gastric acid and pepsin.<\/jats:p>","DOI":"10.1136\/gut.34.7.872","type":"journal-article","created":{"date-parts":[[2007,9,18]],"date-time":"2007-09-18T17:41:31Z","timestamp":1190137291000},"page":"872-880","source":"Crossref","is-referenced-by-count":27,"title":["Measurement of bicarbonate output from the intact human oesophagus."],"prefix":"10.1136","volume":"34","author":[{"given":"C M","family":"Brown","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C F","family":"Snowdon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B","family":"Slee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L N","family":"Sandle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W D","family":"Rees","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1993,7,1]]},"reference":[{"key":"ref_1","first-page":"727","article-title":"Recent views ofthe pathogenesis ofgastro-oesophageal reflux disease","volume":"1","author":"Dent, J.","year":"1987","unstructured":"Dent J. Recent views ofthe pathogenesis ofgastro-oesophageal reflux disease. Clinical Gastroenterology 1987; 1: 727-45.","journal-title":"Clinical Gastroenterology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1020","DOI":"10.1136\/gut.29.8.1020","article-title":"Mechanisms of lower oesophageal sphincter incompetance in patients with symptomatic gastro-oesophageal reflux","volume":"29","author":"Dent, J.; Holloway, R.H.; Toouli, J.; Dodds, W.J.","year":"1988","unstructured":"Dent J, Holloway RH, Toouli J, Dodds WJ. Mechanisms of lower oesophageal sphincter incompetance in patients with symptomatic gastro-oesophageal reflux. 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Gastroenterology 1987; 92: 1393-7.","journal-title":"Gastroenterology"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1016\/0016-5085(89)90635-5","article-title":"In vivo alkaline secretion by mammalian esophagus","volume":"97","author":"Hamilton, B.H.; Orlando, R.C.","year":"1989","unstructured":"Hamilton BH, Orlando RC. In vivo alkaline secretion by mammalian esophagus. Gastroenterology 1989; 97: 640-8.","journal-title":"Gastroenterology"},{"key":"ref_6","first-page":"703","article-title":"Fine structure of squamous epithelium and submucosal glands of human oesophagus. J7 Anat","volume":"123","author":"TM, Al Yassin; PG, Toner","year":"1977","unstructured":"Al Yassin TM, Toner PG. Fine structure of squamous epithelium and submucosal glands of human oesophagus. J7 Anat 1977; 123: 703-21."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1007\/BF01391739","article-title":"A demonstration of bicarbonate production by the normal human stomach","volume":"27","author":"Rees, W.D.W.; Botham, D.; Turnberg, L.A.","year":"1982","unstructured":"Rees WDW, Botham D, Turnberg LA. A demonstration of bicarbonate production by the normal human stomach. Dig Dis Sci 1982; 27: 961-6.","journal-title":"Dig Dis Sci"},{"key":"ref_8","first-page":"370","article-title":"Human duodenal mucosal bicarbonate secretion. Evidence for basal secretion and stimulation by hydrochloric acid and a synthetic","volume-title":"prostaglandin E1 analogue. Gastroenterology 1986; 91","author":"Isenberg, J.I.; Hogan, D.L.; Koss, M.A.; Selling, J.A.","unstructured":"Isenberg JI, Hogan DL, Koss MA, Selling JA. Human duodenal mucosal bicarbonate secretion. Evidence for basal secretion and stimulation by hydrochloric acid and a synthetic prostaglandin E1 analogue. Gastroenterology 1986; 91: 370-8."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1136\/gut.20.11.963","article-title":"Simultaneous measurement of antroduodenal motility, gastric emptying and duodenogastric reflux in man","volume":"20","author":"Rees WDW, Go VLW, Malagelada JR","year":"1979","unstructured":"Rees WDW, Go VLW, Malagelada JR. Simultaneous measurement of antroduodenal motility, gastric emptying and duodenogastric reflux in man. Gut 1979; 20: 963-70.","journal-title":"Gut"},{"key":"ref_10","first-page":"677","article-title":"Evaluation of a colorimetric test for the determination ofa-amylase with p-nitrophenylheptaoside as substrate","volume":"22","author":"Scholer, A.","year":"1984","unstructured":"Scholer A. Evaluation of a colorimetric test for the determination ofa-amylase with p-nitrophenylheptaoside as substrate. J Clin Chem Clin Biochem 1984; 22: 677-84.","journal-title":"J Clin Chem Clin Biochem"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"624","DOI":"10.1177\/000456328602300602","article-title":"Measurement of plasma amylase activity","volume":"23","author":"A, Ying Foo; SB, Rosalki","year":"1986","unstructured":"Ying Foo A, Rosalki SB. Measurement of plasma amylase activity. Ann Clin Biochemn 1986; 23: 624-37.","journal-title":"Ann Clin Biochemn"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1136\/gut.32.10.1093","article-title":"Composition of gastro-oesophageal refluxate","volume":"32","author":"Gotley, D.C.; Morgan, A.P.; Ball, D.; Owen, R.W.; Cooper, M.J.","year":"1991","unstructured":"Gotley DC, Morgan AP, Ball D, Owen RW, Cooper MJ. Composition of gastro-oesophageal refluxate. 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AmJ3 Physiol 1981; 241: G275-88."},{"key":"ref_15","first-page":"718","article-title":"Mechanisms of H+ injury in rabbit esophageal epithelium","volume":"246","author":"Orlando, R.C.; Bryson, J.C.; Powell, D.W.","year":"1984","unstructured":"Orlando RC, Bryson JC, Powell DW. Mechanisms of H+ injury in rabbit esophageal epithelium. AmJ7 Physiol 1984; 246: G718-24.","journal-title":"AmJ7 Physiol"},{"key":"ref_16","article-title":"Regulation of gastrointestinal growth","volume-title":"ofthe gastrointestinal tract","author":"Johnson, L.E.","year":"1987","unstructured":"Johnson LE. Regulation of gastrointestinal growth. In: Johnson LR, ed. Physiology ofthe gastrointestinal tract. New York: Raven Press, 1987, 301."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1016\/0022-4804(88)90179-5","article-title":"Effects of prostaglandins on esophageal blood flow","volume":"44","author":"Duda, G.; Huesken, J.E.; Harmon, J.W.","year":"1988","unstructured":"Duda G, Huesken JE, Harmon JW. Effects of prostaglandins on esophageal blood flow. J Surg Res 1988; 44: 371-81.","journal-title":"J Surg Res"},{"key":"ref_18","first-page":"897","article-title":"Esophageal peristaltic dysfunction","volume-title":"peptic esophagitis. Gastroenterology 1986; 91","author":"Kahrilas, P.J.; Dodds, W.J.; Kern, H.M.; Arndorfer, R.C.; Reece, A.","unstructured":"Kahrilas PJ, Dodds WJ, Kern HM, Arndorfer RC, Reece A. Esophageal peristaltic dysfunction in peptic esophagitis. Gastroenterology 1986; 91: 897-904."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1056\/NEJM198402023100503","article-title":"Effect of esophageal emptying and saliva on clearance of acid from the esophagus","volume":"310","author":"Helm, J.F.; Dodds, W.J.; Pelc, L.R.; Palmer, D.W.; Hogan, W.J.; Teeter, B.C.","year":"1984","unstructured":"Helm JF, Dodds WJ, Pelc LR, Palmer DW, Hogan WJ, Teeter BC. Effect of esophageal emptying and saliva on clearance of acid from the esophagus. N EnglJ3 Med 1984; 310: 284-8.","journal-title":"N EnglJ3 Med"},{"key":"ref_20","first-page":"607","article-title":"Determinants of esophageal acid clearance","volume-title":"normal subjects. Gastroenterology 1983; 85","author":"Helm, J.F.; Dodds, W.J.; Riedel, D.R.; Teeter, B.C.; Hogan, W.J.; Arndorfer, R.C.","unstructured":"Helm JF, Dodds WJ, Riedel DR, Teeter BC, Hogan WJ, Arndorfer RC. Determinants of esophageal acid clearance in normal subjects. 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Their detection mucin, enzyme and secretory protein content","volume":"86","author":"Hopwood, D.; Coghill, G.; Sanders, D.S.A.","year":"1986","unstructured":"Hopwood D, Coghill G, Sanders DSA. Human oesophageal submucosal glands. Their detection mucin, enzyme and secretory protein content. Histochemistry 1986; 86: 107-12.","journal-title":"Histochemistry"},{"key":"ref_24","first-page":"380","article-title":"Site and mechanism of pain perception with oesophageal balloon distension and intravenous edrophonium in patients with oesophageal chest pain","volume":"33","author":"JS, De Caestecker; A, Pryde; RC, Heading","year":"1992","unstructured":"De Caestecker JS, Pryde A, Heading RC. Site and mechanism of pain perception with oesophageal balloon distension and intravenous edrophonium in patients with oesophageal chest pain. Gut 1992; 33: 380-6.","journal-title":"Gut"},{"article-title":"Human oesophageal bicarbonate secretion - a factor in oesophageal acid clearance?","author":"Brown, C.M.; Botham, D.; Rees, W.D.W.","key":"ref_25","unstructured":"Brown CM, Botham D, Rees WDW. Human oesophageal bicarbonate secretion - a factor in oesophageal acid clearance? Gut 1990; 31: Al 192."},{"key":"ref_26","first-page":"126","article-title":"Bicarbonate (HCO3) secretion by human oesophagus","volume":"102","author":"Meyers, R.L.; Orlando, R.C.","year":"1992","unstructured":"Meyers RL, Orlando RC. Bicarbonate (HCO3) secretion by human oesophagus. Gastroenterolog 1992; 102: A126.","journal-title":"Gastroenterolog"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1016\/S0016-5085(82)80021-8","article-title":"Salivary secretion in reflux esophagitis","volume":"83","author":"Sonnenberg, A.; Steinkamp, U.; Weise, A.; Berges, W.; Weinbeck, M.; Rohner, H.G.; Peter, P.","year":"1982","unstructured":"Sonnenberg A, Steinkamp U, Weise A, Berges W, Weinbeck M, Rohner HG, Peter P. Salivary secretion in reflux esophagitis. Gastroenterology 1982; 83: 889-95.","journal-title":"Gastroenterology"},{"key":"ref_28","first-page":"1876","article-title":"pH of the microclimate lining human gastric and duodenal","volume-title":"mucosa in vivo. Gastroenterology 1987; 92","author":"Quigley, E.M.M.; Turnberg, L.A.","unstructured":"Quigley EMM, Turnberg LA. pH of the microclimate lining human gastric and duodenal mucosa in vivo. 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