{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T06:58:14Z","timestamp":1788245894897,"version":"build-2803163510"},"reference-count":29,"publisher":"BMJ","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"published-print":{"date-parts":[[1994,10]]},"abstract":"<jats:p>Permeability tests are widely used to investigate the pathogenesis of various gastrointestinal diseases including coeliac disease, infectious diarrhoea, and inflammatory bowel disease. In Crohn's disease they are used as activity parameters by some investigators. Lack of standardisation, however, makes it very difficult to compare data reported in different studies. The aim of this study was to gather permeation data in well controlled test conditions to standardise the methods. Nine healthy volunteers each received five consecutive permeability tests by mouth using polyethylene glycol-400 (PEG-400) and 51Cr-EDTA as probe molecules. The probes were dissolved in water, a glucose solution, a starch solution, a hyperosmolar lactulose-mannitol solution, and a liquid meal. A significantly decreased permeation for both probes was found when given with the hyperosmolar solution. The 51Cr-EDTA permeation was also decreased with water. The permeability index, 51Cr-EDTA\/PEG-400, corrected for influencing factors, confirmed that the lactulose-mannitol solution and plain water yield lower values of macro-molecule permeation than starch, glucose or liquid meal. Hyperosmolarity was clearly accompanied by a decrease in permeability probably caused by reversed solvent drag. Interindividual variability of probe permeation and permeability index is very low with a standard liquid meal. It is proposed that for permeability studies a standard liquid meal is always used.<\/jats:p>","DOI":"10.1136\/gut.35.10.1404","type":"journal-article","created":{"date-parts":[[2007,9,18]],"date-time":"2007-09-18T17:41:31Z","timestamp":1190137291000},"page":"1404-1408","source":"Crossref","is-referenced-by-count":35,"title":["Test conditions greatly influence permeation of water soluble molecules through the intestinal mucosa: need for standardisation."],"prefix":"10.1136","volume":"35","author":[{"given":"M","family":"Peeters","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Hiele","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y","family":"Ghoos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V","family":"Huysmans","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Geboes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G","family":"Vantrappen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"Rutgeerts","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[1994,10,1]]},"reference":[{"key":"ref_1","first-page":"241","article-title":"Measurements of intestinal permeability using low molecular weight polyethylene glycols (PEG 400). I. Chemical analysis and biological properties","volume-title":"of PEG 400. Gastroenterology","volume":"73","author":"Chadwick, V.S.; Phillips, S.F.; Hoffman, A.F.","year":"1977","unstructured":"Chadwick VS, Phillips SF, Hoffman AF. Measurements of intestinal permeability using low molecular weight polyethylene glycols (PEG 400). I. Chemical analysis and biological properties of PEG 400. Gastroenterology 1977; 73: 241-6."},{"key":"ref_2","first-page":"499","article-title":"Small intestinal permeability in clinical practice","volume-title":"I Clin Gastroenterol","volume":"6","author":"Cooper, B.T.","year":"1984","unstructured":"Cooper BT, Small intestinal permeability in clinical practice. I Clin Gastroenterol 1984; 6: 499-501."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1136\/gut.33.3.320","article-title":"Intestinal permeability in patients with Crohn's disease and their first degree relatives","volume":"33","author":"Teahon, K.; Smethurst, P.; Levi, A.J.; Menzies, I.S.; Bjarnason, I.","year":"1992","unstructured":"Teahon K, Smethurst P, Levi AJ, Menzies IS, Bjarnason I. Intestinal permeability in patients with Crohn's disease and their first degree relatives. Gut 1992; 33: 320-3.","journal-title":"Gut"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1016\/S0009-9120(86)80045-5","article-title":"Studies of intestinal permeability in inflammatory diseases using polyethylene glycol 400","volume":"19","author":"Jenkins, R.T.; Goodacre, R.L.; Rooney, P.J.; Bienenstock, J.; Sivakumaran, T.; Walker, W.H.C.","year":"1986","unstructured":"Jenkins RT, Goodacre RL, Rooney PJ, Bienenstock J, Sivakumaran T, Walker WHC. Studies of intestinal permeability in inflammatory diseases using polyethylene glycol 400. Clin Biochem 1986; 19: 298-302.","journal-title":"Clin Biochem"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/0021-9673(93)80193-C","article-title":"Analysis of multiple sugar probes in urine and plasma by high-performance anionexchange chromatography with pulsed electrochemical detection. Application in the assessment of intestinal permeability in human immunodeficiency virus infection","volume":"640","author":"Fleming, S.C.; Kynaston, J.A.; Laker, M.F.; Pearson, A.D.; Kapembwa, M.S.; Griffin, G.E.","year":"1993","unstructured":"Fleming SC, Kynaston JA, Laker MF, Pearson AD, Kapembwa MS, Griffin GE. Analysis of multiple sugar probes in urine and plasma by high-performance anionexchange chromatography with pulsed electrochemical detection. Application in the assessment of intestinal permeability in human immunodeficiency virus infection. J Chromatogr 1993; 640: 293-7.","journal-title":"J Chromatogr"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1136\/gut.31.4.417","article-title":"Regulation of polyethylene glycol 400 intestinal permeability by endogenous and exogenous prostanoids. Influence of non-steroidal anti-inflammatory drugs","volume":"31","author":"Krugliak, P.; Hollander, D.; Le, K.; Ma, T.; Dadfalza, V.D.; Katz, K.D.","year":"1990","unstructured":"Krugliak P, Hollander D, Le K, Ma T, Dadfalza VD, Katz KD. Regulation of polyethylene glycol 400 intestinal permeability by endogenous and exogenous prostanoids. Influence of non-steroidal anti-inflammatory drugs. Gut 1990; 31: 417-21.","journal-title":"Gut"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1136\/gut.32.1.66","article-title":"Do non-steroidal anti-inflammatory drugs increase colonic permeability?","volume":"32","author":"Jenkins, A.P.; Trew, D.R.; Crump, B.J.; Nukajam, W.S.; Foley, J.A.; Menzies, I.S.","year":"1991","unstructured":"Jenkins AP, Trew DR, Crump BJ, Nukajam WS, Foley JA, Menzies IS, et al. Do non-steroidal anti-inflammatory drugs increase colonic permeability? Gut 1991; 32: 66-9.","journal-title":"Gut"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1097\/00005176-198910000-00007","article-title":"Low-dose methotrexate induced changes in intestinal permeability determined by polyethylene glycol polymers","volume":"9","author":"Lifschitz, C.H.; Mahoney, D.H.","year":"1989","unstructured":"Lifschitz CH, Mahoney DH. Low-dose methotrexate induced changes in intestinal permeability determined by polyethylene glycol polymers. J Pediatr Gastroenterol Nutr 1989; 9: 301-6.","journal-title":"J Pediatr Gastroenterol Nutr"},{"key":"ref_9","first-page":"163","article-title":"Smoking and intestinal absorption of oral polyethylene glycols in Crohn's disease","volume":"28","author":"Soderholm, J.; Olaison, G.; Sjodahl, R.; Tagesson, C.","year":"1993","unstructured":"Soderholm J, Olaison G, Sjodahl R, Tagesson C. Smoking and intestinal absorption of oral polyethylene glycols in Crohn's disease. Scand J7 Gastroenterol 1993; 28: 163-7."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"351","DOI":"10.3109\/00365528909093059","article-title":"5ICr-Ethylenediaminetetraacetic acid absorption test methodologic aspects","volume":"24","author":"Aabakken, L.","year":"1989","unstructured":"Aabakken L. 5ICr-Ethylenediaminetetraacetic acid absorption test methodologic aspects. Scand J Gastroenterol 1989; 24: 351-8.","journal-title":"Scand J Gastroenterol"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1042\/cs0750375","article-title":"Comparison between the cellobiose\/mannitol and 51Cr-EDTA absorption tests in the detection of coeliac disease","volume":"75","author":"Martines, D.; Morris, A.I.; Gilmore, I.T.; Williams, A.; Stockdale, H.; Critchley, M.","year":"1988","unstructured":"Martines D, Morris AI, Gilmore IT, Williams A, Stockdale H, Critchley M, et al. Comparison between the cellobiose\/mannitol and 51Cr-EDTA absorption tests in the detection of coeliac disease. Clin Science 1988; 75: 375-8.","journal-title":"Clin Science"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/0016-5085(89)90767-1","article-title":"Lactulose\/mannitol: an ideal screen for celiac disease","volume":"96","author":"Juby, L.D.; Rothwell, J.; Axon, A.T.R.","year":"1989","unstructured":"Juby LD, Rothwell J, Axon ATR. 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A hypothesis as to its regulation and involvement in Crohn's disease (review)","volume":"27","author":"Hollander, D.","year":"1992","unstructured":"Hollander D. The intestinal permeability barrier. A hypothesis as to its regulation and involvement in Crohn's disease (review). ScandJ Gastroenterol 1992; 27: 721-6.","journal-title":"ScandJ Gastroenterol"},{"key":"ref_20","first-page":"1445","article-title":"Comparison of different measurements of intestinal permeability in inflammatory bowel","volume":"86","author":"Howden, G.W.; Robertson, C.; Duncan, A.; Movis, A.J.; Russell, R.I.","year":"1991","unstructured":"Howden GW, Robertson C, Duncan A, Movis AJ, Russell RI. Comparison of different measurements of intestinal permeability in inflammatory bowel. Am J Gastroenterol 1991; 86: 1445-9.","journal-title":"Am J Gastroenterol"},{"key":"ref_21","first-page":"993","article-title":"Intestinal permeability of 5ICr-EDTA in patients with Crohn's disease and their healthy relatives","volume":"24","author":"Ainsworth, M.; Eriksen, J.; Rasmussen, J.W.; Schaffalitzky de Muckadell, O.B.","year":"1989","unstructured":"Ainsworth M, Eriksen J, Rasmussen JW, Schaffalitzky de Muckadell OB. Intestinal permeability of 5ICr-EDTA in patients with Crohn's disease and their healthy relatives. ScandJ7 Gastroenterol 1989; 24: 993-8."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1111\/j.1365-2362.1988.tb02404.x","article-title":"Gastrointestinal permeability to polyethylene glycol: an evaluation of urinary recovery of an oral load of polyethylene glycol as a parameter of intestinal permeability in man","volume":"18","author":"Philipsen, E.K.; Batsberg, W.; Christensen, A.B.","year":"1988","unstructured":"Philipsen EK, Batsberg W, Christensen AB. Gastrointestinal permeability to polyethylene glycol: an evaluation of urinary recovery of an oral load of polyethylene glycol as a parameter of intestinal permeability in man. EurJ Clin Invest 1988; 18: 139-45.","journal-title":"EurJ Clin Invest"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1136\/gut.26.6.579","article-title":"Intestinal permeability to 5ICr-EDTA in rats with experimentally induced enteropathy","volume":"26","author":"Bjarnason, I.; Smethurst, P.; Levi, A.J.; Peters, T.J.","year":"1985","unstructured":"Bjarnason I, Smethurst P, Levi AJ, Peters TJ. Intestinal permeability to 5ICr-EDTA in rats with experimentally induced enteropathy. Gut 1985; 26: 579-85.","journal-title":"Gut"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1164","DOI":"10.1016\/0016-5085(89)91686-7","article-title":"Mechanisms of polyethylene glycol 400 permeability of perfused rat intestine","volume":"97","author":"Krugliak, P.; Hollander, D.; Ma, T.Y.; Tran, D.; Dadufalza, V.D.; Katz, K.D.","year":"1989","unstructured":"Krugliak P, Hollander D, Ma TY, Tran D, Dadufalza VD, Katz KD, et al. Mechanisms of polyethylene glycol 400 permeability of perfused rat intestine. Gastroenterology 1989;97: 1164-70.","journal-title":"Gastroenterology"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1007\/BF02209147","article-title":"Structural basis for physiological regulation of paracellular pathways in intestinal epithelia","volume":"100","author":"Madara, J.L.; Pappenheimer, JR","year":"1987","unstructured":"Madara JL, Pappenheimer JR. Structural basis for physiological regulation of paracellular pathways in intestinal epithelia. Jf Membr Biol 1987; 100: 149-64.","journal-title":"Jf Membr Biol"},{"key":"ref_26","first-page":"290","article-title":"Paracellular intestinal absorption of glucose, creatinine, and mannitol in normal animals: relation to body size","volume":"259","author":"Pappenheimer, JR","year":"1990","unstructured":"Pappenheimer JR. Paracellular intestinal absorption of glucose, creatinine, and mannitol in normal animals: relation to body size. Am J7 Physiol 1990; 259: G290-9.","journal-title":"Am J7 Physiol"},{"key":"ref_27","first-page":"1117","article-title":"Effect of D-glucose on intestinal permeability and its passive absorption","volume-title":"human small intestine in vivo. Gastroenterology 1993; 105","author":"Fine, K.D.; Santa Ana, C.A.; Porter, J.L.; Fordtran, J.S.","unstructured":"Fine KD, Santa Ana CA, Porter JL, Fordtran JS. Effect of D-glucose on intestinal permeability and its passive absorption in human small intestine in vivo. Gastroenterology 1993; 105: 1117-25."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1042\/cs0730197","article-title":"Evaluation of mannitol, lactulose and 5ICr-labelled ethylenediaminetetra-acetate as markers of intestinal permeability in man","volume":"73","author":"Elia, M.; Behrens, R.; Northrop, C.; Wraight, P.; Neale, G.","year":"1987","unstructured":"Elia M, Behrens R, Northrop C, Wraight P, Neale G. Evaluation of mannitol, lactulose and 5ICr-labelled ethylenediaminetetra-acetate as markers of intestinal permeability in man. Clin Science 1987; 73: 197-204.","journal-title":"Clin Science"},{"key":"ref_29","first-page":"1159","article-title":"Reversibility of increased intestinal permeability to 51Cr-EDTA in patients with gastrointestinal inflammatory diseases","volume":"82","author":"Jenkins, R.T.; Jones, D.B.; Goodacre, R.L.; Collins, S.M.; Coates, G.; Hunt, R.H.","year":"1987","unstructured":"Jenkins RT, Jones DB, Goodacre RL, Collins SM, Coates G, Hunt RH, et al. Reversibility of increased intestinal permeability to 51Cr-EDTA in patients with gastrointestinal inflammatory diseases. Am J Gastroenterol 1987; 82: 1159-64.","journal-title":"Am J Gastroenterol"}],"container-title":["Gut"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/gut.35.10.1404","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T09:53:01Z","timestamp":1765619581000},"score":1,"resource":{"primary":{"URL":"https:\/\/gut.bmj.com\/lookup\/doi\/10.1136\/gut.35.10.1404"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,10]]},"references-count":29,"journal-issue":{"issue":"10","published-online":{"date-parts":[[1994,10,1]]},"published-print":{"date-parts":[[1994,10]]}},"alternative-id":["10.1136\/gut.35.10.1404"],"URL":"https:\/\/doi.org\/10.1136\/gut.35.10.1404","relation":{},"ISSN":["0017-5749","1468-3288"],"issn-type":[{"value":"0017-5749","type":"print"},{"value":"1468-3288","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,10]]}}}