{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T09:40:10Z","timestamp":1790502010167,"version":"4.1.0"},"reference-count":31,"publisher":"BMJ","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"published-print":{"date-parts":[[1994,4]]},"abstract":"<jats:p>Immunohistochemistry of the S phase related proliferating cell nuclear antigen (PCNA) was studied as an alternative to ex-vivo bromodeoxyuridine (BrdU) immunohistochemistry for assessment of human colonic cell proliferation. From 16 subjects without colonic disease biopsy specimens were collected from five different sites along the colorectum and processed for BrdU and PCNA immunohistochemistry. The mean proliferation index of PCNA was significantly higher at 133% of the value obtained with BrdU. There was, however, a good correlation between the results from both techniques (r = 0.6275; p &lt; 0.05). Decrease in proliferation index along the colorectum was seen with both staining methods but was clearer with PCNA immunohistochemistry (caecum\/ascending colon v rectum: 12.0 v 7.2; p &lt; 0.004). The total number of crypt cells also decreased from proximal to distal (134 to 128; p &lt; 0.06) but at no site correlated significantly with the proliferation index. It is concluded that in clinical cell kinetic studies staining for PCNA may serve as an attractive alternative to the BrdU incorporation assay.<\/jats:p>","DOI":"10.1136\/gut.35.4.530","type":"journal-article","created":{"date-parts":[[2007,9,18]],"date-time":"2007-09-18T17:41:31Z","timestamp":1190137291000},"page":"530-535","source":"Crossref","is-referenced-by-count":157,"title":["Proliferating cell nuclear antigen (PCNA): a new marker to study human colonic cell proliferation."],"prefix":"10.1136","volume":"35","author":[{"given":"F J","family":"Kubben","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Peeters-Haesevoets","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L G","family":"Engels","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C G","family":"Baeten","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B","family":"Schutte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J W","family":"Arends","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R W","family":"Stockbr\u00fcgger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G H","family":"Blijham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[1994,4,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1136","DOI":"10.1002\/1097-0142(19820915)50:6<1136::AID-CNCR2820500617>3.0.CO;2-A","article-title":"Significance of labelling index and labelling distribution on kinetic parameters in colorectal mucosa of cancer patients and DMH-treated animals","volume":"50","author":"Deschner, E.E.; Moshen, A.P.","year":"1982","unstructured":"Deschner EE, Moshen AP. 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It was found that the crypt height, expressed as the number of cells\/colonic crypt was constant over the colorectum and in individual cases did not correlate with the markers of proliferation. It remains to be investigated if the crypt height is an alternative or additional biomarker for the risk of developing colorectal adenoma or carcinoma. In summary, in the human colorectum the rate and site of cell proliferation determined by immunostaining for PCNA reflects the prolifera-","author":"van Dierendock J-H; R, Keyzer; van de Velde CJH; CJ, Cornelisse","unstructured":"van Dierendock J-H, Keyzer R, van de Velde CJH, Cornelisse CJ. Subdivision of S-phase by analysis of nuclear 5bromodeoxyuridine staining patterns. Cytometry 1989; 10: labelling index or PCNA staining index. It was found that the crypt height, expressed as the number of cells\/colonic crypt was constant over the colorectum and in individual cases did not correlate with the markers of proliferation. 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