{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T04:31:58Z","timestamp":1764995518414,"version":"3.46.0"},"reference-count":7,"publisher":"BMJ","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"published-print":{"date-parts":[[1994,7]]},"abstract":"<jats:p>Barrett's oesophagus is a preneoplastic condition in which the squamous mucosa of the oesophagus is replaced by columnar epithelium. Epithelial cells of Barrett's oesophagus were isolated from resected oesophagus specimens by two methods not previously applied to the culture of Barrett's oesophagus cells. These techniques included trypsinisation of small fragments of mucosa, followed by plating in tissue culture dishes, and a direct tissue explant technique. A modified MCDB-153 growth medium was used. Primary trypsin technique cultures were plated on uncoated plastic, or plastic coated with type I collagen, type IV collagen, or fibronectin. Growth on type IV collagen and fibronectin plates was slower but produced less contamination from fibroblasts. By 20-40 days most cultures formed confluent monolayers made up of cells with epithelial morphology. The cells were cytokeratin positive, vimentin negative, and contained alcian blue positive vacuoles, confirming their epithelial origin and suggesting their derivation from Barrett's oesophagus. Electron microscopy showed tonofilaments, microvilli, and desmosomes. Cells proliferated through up to eight subcultures before growth slowed and cells showed senescent changes. This study shows that epithelial cells from Barrett's oesophagus can be grown by comparatively simple tissue culture techniques. These methods can provide sufficient material for a variety of molecular biology and biochemical studies of epithelial cells from Barrett's oesophagus.<\/jats:p>","DOI":"10.1136\/gut.35.7.879","type":"journal-article","created":{"date-parts":[[2007,9,18]],"date-time":"2007-09-18T17:41:31Z","timestamp":1190137291000},"page":"879-883","source":"Crossref","is-referenced-by-count":8,"title":["Tissue culture of epithelium derived from Barrett's oesophagus."],"prefix":"10.1136","volume":"35","author":[{"given":"K","family":"Washington","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M R","family":"Gottfried","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M J","family":"Telen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1994,7,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/S0046-8177(88)80344-7","article-title":"Observer variation in the diagnosis of dysplasia in Barrett's esophagus","volume":"19","author":"Reid, B.J.; Haggitt, R.C.; Rubin, C.E.; Roth, G.; Surawicz, C.M.; Van Belle, G.","year":"1988","unstructured":"Reid BJ, Haggitt RC, Rubin CE, Roth G, Surawicz CM, Van Belle G, et al. 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Tissue culture of epithelial cells from esophageal specialized columnar epithelium (Barrett's esophagus). Dig Dis Sci 1992; 37: 532-6.","journal-title":"Dig Dis Sci"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/S0074-7696(08)61743-X","article-title":"Origin and ultrastructure of cells in vitro","volume":"48","author":"Franks, L.M.; Wilson, P.D.","year":"1977","unstructured":"Franks LM, Wilson PD. Origin and ultrastructure of cells in vitro. Int Rev Cytol 1977; 48: 55-139.","journal-title":"Int Rev Cytol"},{"key":"ref_6","first-page":"3288","article-title":"Ornithine decarboxylase and polyamine levels in columnar upper gastrointestinal mucosae in patients with Barrett's esophagus","volume":"48","author":"Garewal, H.S.; Gerner, E.W.; Sampliner, R.E.; Roe, D.","year":"1988","unstructured":"Garewal HS, Gerner EW, Sampliner RE, Roe D. 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J Cell Physiol 1982; 110: 219-29.","journal-title":"J Cell Physiol"}],"container-title":["Gut"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/gut.35.7.879","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T03:55:06Z","timestamp":1764993306000},"score":1,"resource":{"primary":{"URL":"https:\/\/gut.bmj.com\/lookup\/doi\/10.1136\/gut.35.7.879"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,7]]},"references-count":7,"journal-issue":{"issue":"7","published-online":{"date-parts":[[1994,7,1]]},"published-print":{"date-parts":[[1994,7]]}},"alternative-id":["10.1136\/gut.35.7.879"],"URL":"https:\/\/doi.org\/10.1136\/gut.35.7.879","relation":{},"ISSN":["0017-5749","1468-3288"],"issn-type":[{"type":"print","value":"0017-5749"},{"type":"electronic","value":"1468-3288"}],"subject":[],"published":{"date-parts":[[1994,7]]}}}