{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T07:54:55Z","timestamp":1765007695911,"version":"3.46.0"},"reference-count":22,"publisher":"BMJ","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"published-print":{"date-parts":[[1994,8]]},"abstract":"<jats:p>The effect of depriving the intestine of bile for 48 hours was studied to determine any influence on various parameters of innate immunity in the gastrointestinal tract. Groups of rats were prepared by bile duct cannulation (with or without fluid replacement) or bile duct ligation. Normal and sham operated animals were used for comparing the thickness of the mucus layer and the cells contained therein, enumeration of goblet cells, and measurement of villus size. Histological examination indicated that the intestinal tissues of treated and control rats were similar. Though villus size and numbers of goblet cells were unaffected, a significant reduction occurred in the thickness of the mucus blanket in the duodenal regions of rats deprived of bile, and there were significantly lower numbers of mucus associated enterocytes and lymphocytes, suggesting a lower turnover rate of the epithelium. The balance of the bacterial populations in the caecum and intestine was altered by bile deprivation-increased numbers of coliform organisms were found in both regions. The range of factors, including antibodies and other known constituents, present in bile may contribute to the maintenance of tissue integrity and influence the balance in indigenous bacterial populations in the intestine. Disturbance of the host's biliary system and concomitant effects on the microbial flora may weaken the overall processes of defence in the intestine.<\/jats:p>","DOI":"10.1136\/gut.35.8.1047","type":"journal-article","created":{"date-parts":[[2007,9,18]],"date-time":"2007-09-18T17:41:31Z","timestamp":1190137291000},"page":"1047-1052","source":"Crossref","is-referenced-by-count":11,"title":["Role of bile in non-specific defence mechanisms of the gut."],"prefix":"10.1136","volume":"35","author":[{"given":"T","family":"Kalambaheti","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G N","family":"Cooper","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G D","family":"Jackson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1994,8,1]]},"reference":[{"key":"ref_1","first-page":"315","article-title":"In defence of mucosal surfaces. Development of novel vaccines for IgA responses protective at the portals of entry of microbial pathogens","volume":"4","author":"McGhee, JR, Mestecky; J.","year":"1990","unstructured":"McGhee JR, Mestecky J. In defence of mucosal surfaces. Development of novel vaccines for IgA responses protective at the portals of entry of microbial pathogens. Infect Dis Clin North Am 1990; 4: 315-41.","journal-title":"Infect Dis Clin North Am"},{"key":"ref_2","first-page":"19","article-title":"Immunoglobulin and antibody in gall bladder bile","volume":"54","year":"1970","unstructured":"Keclik M, Wolf RH, Felsenfeld 0, Smetana HF. Immunoglobulin and antibody in gall bladder bile. Am J Gastroenterol 1970; 54: 19-29.","journal-title":"Am J Gastroenterol"},{"key":"ref_3","first-page":"123","article-title":"Rapid disappearance from serum of intravenously injected rat myeloma IgA and its detection in bile","volume":"8","author":"Jackson, G.D.F.; Lemaitre-Coelho, I.; Vaerman, J.P.; Bazin, H.; Beckers, A.","year":"1978","unstructured":"Jackson GDF, Lemaitre-Coelho I, Vaerman JP, Bazin H, Beckers A. Rapid disappearance from serum of intravenously injected rat myeloma IgA and its detection in bile. EurJImmunol 1978; 8: 123-6.","journal-title":"EurJImmunol"},{"key":"ref_4","first-page":"397","article-title":"Further evidence that hepatic sources confer biliary antibody in the rat","volume":"76","author":"Jackson, G.D.F.; Hansen, P.G.C.; Underdown, B.J.","year":"1992","unstructured":"Jackson GDF, Hansen PGC, Underdown BJ. Further evidence that hepatic sources confer biliary antibody in the rat. Immunology 1992; 76: 397-401.","journal-title":"Immunology"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1022","DOI":"10.1016\/S0016-5085(84)80060-8","article-title":"Occurrence of specific secretory immunoglobulin A in bile after inoculation of Giardia lamblia trophozoites into rat duodenum","volume":"87","author":"Loftness, T.J.; Erlandsen, S.L.; Wilson, I.D.; Meyer, E.A.","year":"1984","unstructured":"Loftness TJ, Erlandsen SL, Wilson ID, Meyer EA. Occurrence of specific secretory immunoglobulin A in bile after inoculation of Giardia lamblia trophozoites into rat duodenum. Gastroenterology 1984; 87: 1022-9.","journal-title":"Gastroenterology"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/0165-2478(88)90069-7","article-title":"Cholera toxin neutralization: a comparison of purified IgG and biliary secretory IgA antibodies","volume":"18","author":"Pierre, P.; Langendries, A.; Vaerman, J.P.","year":"1988","unstructured":"Pierre P, Langendries A, Vaerman JP. Cholera toxin neutralization: a comparison of purified IgG and biliary secretory IgA antibodies. Immunol Lett 1988; 18: 51-6.","journal-title":"Immunol Lett"},{"key":"ref_7","first-page":"101","article-title":"Le Quesne LP. Gallstone disease presenting as septicaemic shock","volume-title":"BrJ7Surg","volume":"65","author":"Faber, R.G.; Ibrahim, S.Z.; Thomas, D.M.; Beyon, G.P.J.","year":"1978","unstructured":"Faber RG, Ibrahim SZ, Thomas DM, Beyon GPJ, Le Quesne LP. Gallstone disease presenting as septicaemic shock. BrJ7Surg 1978; 65: 101-5."},{"issue":"98","key":"ref_8","first-page":"629","article-title":"The significance of biliary pressure in cholangitis","volume":"1969","author":"Huang, T.; Bass, J.A.; William, R.D.","unstructured":"Huang T, Bass JA, William RD. The significance of biliary pressure in cholangitis. Arch Surg 1969; 98: 629-32.","journal-title":"Arch Surg"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1042\/cs0630187","article-title":"A simple method for measuring thickness of the mucus gel layer adherent to rat, frog and human gastric mucosa: influence of feeding, prostaglandin, N-acetylcysteine and other agents","volume":"63","author":"Keress, S.; Allen, A.; Garner, A.","year":"1982","unstructured":"Keress S, Allen A, Garner A. A simple method for measuring thickness of the mucus gel layer adherent to rat, frog and human gastric mucosa: influence of feeding, prostaglandin, N-acetylcysteine and other agents. Clin Sci 1982; 63: 187-95.","journal-title":"Clin Sci"},{"key":"ref_10","first-page":"15","article-title":"Factors influencing villus size in the small intestine of adult rats as revealed by transposition of intestinal segments","volume":"127","author":"Altmann, G.G.; Leblond, C.P.","year":"1970","unstructured":"Altmann GG, Leblond CP. Factors influencing villus size in the small intestine of adult rats as revealed by transposition of intestinal segments. AmJAnat 1970; 127: 15-36.","journal-title":"AmJAnat"},{"key":"ref_11","first-page":"59","article-title":"The development of the bacterial flora in the gastrointestinal tract of mice","volume":"122","author":"Schaedler, R.W.; Dubos, R.; Costello, R.","year":"1965","unstructured":"Schaedler RW, Dubos R, Costello R. The development of the bacterial flora in the gastrointestinal tract of mice. JfExp Med 1965; 122: 59-66.","journal-title":"JfExp Med"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1038\/icb.1978.73","article-title":"The mucosa-associated microflora of the rat intestine: a study of normal distribution and magnesium sulphate induced diarrhoea","volume":"56","author":"Phillips, M.; Lee, A.; Leach, W.D.","year":"1978","unstructured":"Phillips M, Lee A, Leach WD. The mucosa-associated microflora of the rat intestine: a study of normal distribution and magnesium sulphate induced diarrhoea. Aust JfExp BiolMed Sci 1978; 56: 649-62.","journal-title":"Aust JfExp BiolMed Sci"},{"key":"ref_13","first-page":"133","article-title":"Antibacterial immunity to Vibrio cholerae in rats","volume":"22","author":"Cooper, G.N.; Narendranathan, R.","year":"1986","unstructured":"Cooper GN, Narendranathan R. Antibacterial immunity to Vibrio cholerae in rats. JfMedMicrobiol 1986; 22: 133-41.","journal-title":"JfMedMicrobiol"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1007\/978-1-4615-9412-3_3","article-title":"Neglected niches: The microbial ecology of the gastrointestinal tract","volume":"8","author":"Lee, A.","year":"1985","unstructured":"Lee A. Neglected niches: The microbial ecology of the gastrointestinal tract. Advances in Microbial Ecology 1985; 8: 115-62.","journal-title":"Advances in Microbial Ecology"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1842","DOI":"10.1016\/0016-5085(92)91443-8","article-title":"Regulation of canine gastric mucin synthesis and phospholipid secretion by acid secretagogues","volume":"103","author":"Scheiman, J.M.; Kraus, E.R.; Boland, C.R.","year":"1992","unstructured":"Scheiman JM, Kraus ER, Boland CR. Regulation of canine gastric mucin synthesis and phospholipid secretion by acid secretagogues. Gastroenterology 1992; 103: 1842-50.","journal-title":"Gastroenterology"},{"key":"ref_16","article-title":"Influence ofbile on parameters ofinnate immunity in the rat gastrointestinal tract","author":"Kalambaheti, R.","year":"1991","unstructured":"Kalambaheti R. Influence ofbile on parameters ofinnate immunity in the rat gastrointestinal tract. University of New South Wales, Sydney, 1991. (MSc dissertation.)"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1093\/clinids\/9.1.28","article-title":"Traditional enteropathogenic Escherichia coli of infantile diarrhea","volume":"9","author":"Robins-Browne, R.M.","year":"1987","unstructured":"Robins-Browne RM. Traditional enteropathogenic Escherichia coli of infantile diarrhea. Rev Infect Dis 1987; 9: 28-53.","journal-title":"Rev Infect Dis"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1159\/000181104","article-title":"Effects of estradiol and progesterone on epidermal growth factor concentration in plasma, bile, urine, submandibular gland and kidney of the mouse","volume":"31","author":"Tuomela, T.; Vunikka, L.; Perheentupa, J.","year":"1989","unstructured":"Tuomela T, Vunikka L, Perheentupa J. Effects of estradiol and progesterone on epidermal growth factor concentration in plasma, bile, urine, submandibular gland and kidney of the mouse. Horm Res 1989; 31: 143-7.","journal-title":"Horm Res"},{"key":"ref_19","first-page":"1300","article-title":"Role of epidermal growth factor in healing of chronic gastroduodenal","volume-title":"ulcers in rats. Gastroenterology 1988; 94","author":"Konturek, S.J.; Dembinski, A.; Warzencha, Z.; Brozozowski, T.; Gregory, H.","unstructured":"Konturek SJ, Dembinski A, Warzencha Z, Brozozowski T, Gregory H. Role of epidermal growth factor in healing of chronic gastroduodenal ulcers in rats. Gastroenterology 1988; 94: 1300-7."},{"key":"ref_20","first-page":"215","article-title":"The nature and sources of natural biliary immunoglobulins. Protides of the biologicalfluids","volume":"36","author":"Jackson, G.D.F.; Hansen, P.G.C.; Manning, R.J.","year":"1989","unstructured":"Jackson GDF, Hansen PGC, Manning RJ. The nature and sources of natural biliary immunoglobulins. Protides of the biologicalfluids 1989; 36: 215-22."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/0016-5085(84)90141-0","article-title":"Studies on the origin of biliary immunoglobulins in rats","volume":"87","author":"Manning, R.J.; Walker, P.G.; Carter, L.; Barrington, P.J.; Jackson, G.D.F.","year":"1984","unstructured":"Manning RJ, Walker PG, Carter L, Barrington PJ, Jackson GDF. Studies on the origin of biliary immunoglobulins in rats. Gastroenterology 1984; 87: 173-9.","journal-title":"Gastroenterology"},{"key":"ref_22","first-page":"305","article-title":"Absence of intestinal bile promotes bacterial translocation","volume":"58","author":"Slocum, M.M.; Sitting, K.M.; Specian, R.D.; Deitch, E.A.","year":"1992","unstructured":"Slocum MM, Sitting KM, Specian RD, Deitch EA. Absence of intestinal bile promotes bacterial translocation. 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