{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T04:16:57Z","timestamp":1764994617101,"version":"3.46.0"},"reference-count":24,"publisher":"BMJ","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"published-print":{"date-parts":[[1995,4]]},"abstract":"<jats:p>Impaired secretion of lithostathine, a pancreatic glycoprotein capable of inhibiting the growth of CaCO3 crystals, has been reported in chronic calcifying pancreatitis. Controversial results were obtained, however, using immunoassays with different antibodies. The aim of this study was to purify and to measure juice lithostathine by a non-immunological method. Fast protein liquid chromatography (FPLC) on a cation exchange column eluted by a sodium chloride gradient, was used. The conditions appropriate to separate secretory (S) from hydrolysed (H) isoforms of immunopurified lithostathine were also used for juice analysis. Pancreatic juice was collected by endoscopic cannulation of the major pancreatic duct, after secretin stimulation, from eight patients with chronic pancreatitis (CP) and from eight controls. In all samples, S-isoforms of lithostathine (ranging from 16 to 19 Mr at SDS-PAGE) were the only constituent of two of the 15 peaks in which FPLC resolved the pancreatic proteins. The nature of these two peaks was confirmed by their coelution with immunopurified S-lithostathine and by immunoblot analysis with polyclonal anti-lithostathine antibodies. The ratio between the area of S-lithostathine peaks and the total area of proteic eluates, was always lower in CP patients (5.3 micrograms\/mg of protein, median value; 0.2-15.4, range) than in controls (35.2 micrograms\/mg; 16.6-55.9). It is concluded that lithostathine can be purified and measured in pancreatic juice by FPLC. Our results with a nonimmunological assay confirm a reduced secretion of lithostathine in patients with CP.<\/jats:p>","DOI":"10.1136\/gut.36.4.622","type":"journal-article","created":{"date-parts":[[2007,9,18]],"date-time":"2007-09-18T17:41:31Z","timestamp":1190137291000},"page":"622-629","source":"Crossref","is-referenced-by-count":7,"title":["Purification and assay of secretory lithostathine in human pancreatic juice by fast protein liquid chromatography."],"prefix":"10.1136","volume":"36","author":[{"given":"A","family":"Mariani","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G","family":"Mezzi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Malesci","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1995,4,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1176","DOI":"10.1016\/S0006-291X(79)80031-5","article-title":"Characterization of a protein isolated from pancreatic calculi of men suffering from chronic calcifying pancreatitis","volume":"87","author":"A, De Caro; J, Lohse; H, Sarles","year":"1979","unstructured":"De Caro A, Lohse J, Sarles H. Characterization of a protein isolated from pancreatic calculi of men suffering from chronic calcifying pancreatitis. Biochem Biophys Res Commun 1979; 87: 1176-82.","journal-title":"Biochem Biophys Res Commun"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1277","DOI":"10.1016\/0016-5085(92)91516-7","article-title":"Inhibition of nucleation and crystal growth of calcium carbonate by human lithostathine","volume":"103","author":"Bernard, J.P.; Adrich, Z.; Montalto, G.; De Caro, A.; De Reggi, M.; Sarles, H.","year":"1992","unstructured":"Bernard JP, Adrich Z, Montalto G, De Caro A, De Reggi M, Sarles H, et al. Inhibition of nucleation and crystal growth of calcium carbonate by human lithostathine. Gastroenterology 1992; 103: 1277-84.","journal-title":"Gastroenterology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1042\/bj2380227","article-title":"Partial amino acid sequence of human pancreatic stone protein, a novel pancreatic secretory protein","volume":"238","author":"Montalto, G.; Bonicel, J.; Multigner, L.; Rovery, M.; Sarles, H.; De Caro, A.","year":"1986","unstructured":"Montalto G, Bonicel J, Multigner L, Rovery M, Sarles H, De Caro A. Partial amino acid sequence of human pancreatic stone protein, a novel pancreatic secretory protein. BiochemJt 1986; 238: 227-32.","journal-title":"BiochemJt"},{"key":"ref_4","first-page":"201","article-title":"Complete amino acid sequence of an immunoreactive form of human pancreatic stone protein, a novel pancreatic secretory protein","volume":"168","author":"A, De Caro; J, Bonicel; P, Rouimi; J, De Caro; H, Sarles; M, Rovery","year":"1986","unstructured":"De Caro A, Bonicel J, Rouimi P, De Caro J, Sarles H, Rovery M. Complete amino acid sequence of an immunoreactive form of human pancreatic stone protein, a novel pancreatic secretory protein. EurJ Biochem 1986; 168: 201-7.","journal-title":"EurJ Biochem"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/0014-5793(87)80688-9","article-title":"Cleavage of the Arg-Ile bond in the native polypeptide chain of human pancreatic stone protein","volume":"216","author":"Rouimi, P.; Bonicel, J.; Rovery, M.; De Caro, A.","year":"1987","unstructured":"Rouimi P, Bonicel J, Rovery M, De Caro A. Cleavage of the Arg-Ile bond in the native polypeptide chain of human pancreatic stone protein. FEBS Lett 1987; 216: 195-9.","journal-title":"FEBS Lett"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/0167-4838(89)90305-1","article-title":"N-terminal sequence extension in the glycosylated forms of human pancreatic stone protein. The 5 oxoproline N-terminal chain is 0 glycosylated on the 5th amino acid residue","volume":"994","author":"A, De Caro; Z, Adrich; B, Fournet; C, Capon; J, Bonicel; J, Ce Caro","year":"1989","unstructured":"De Caro A, Adrich Z, Fournet B, Capon C, Bonicel J, Ce Caro J, et al. N-terminal sequence extension in the glycosylated forms of human pancreatic stone protein. The 5 oxoproline N-terminal chain is 0 glycosylated on the 5th amino acid residue. Biochim Biophys Acta 1989; 994: 281-4.","journal-title":"Biochim Biophys Acta"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2115","DOI":"10.1172\/JCI112216","article-title":"Isolation, characterization and distribution of an unusual pancreatic human secretory protein","volume":"76","author":"Gross, J.; Carlson, R.I.; Brauer, A.W.; Margolies, M.N.; Warshaw, A.L.; Wands, JR","year":"1985","unstructured":"Gross J, Carlson RI, Brauer AW, Margolies MN, Warshaw AL, Wands JR. Isolation, characterization and distribution of an unusual pancreatic human secretory protein. JClin Invest 1985; 76: 2115-26.","journal-title":"JClin Invest"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1016\/0016-5085(85)90341-5","article-title":"Pancreatic stone protein II. Implication in stone formation during the course of chronic calcifying pancreatitis","volume":"89","author":"Multigner, L.; Sarles, H.; Lombardo, D.; DeCaro, A.","year":"1985","unstructured":"Multigner L, Sarles H, Lombardo D, DeCaro A. Pancreatic stone protein II. Implication in stone formation during the course of chronic calcifying pancreatitis. Gastroenterology 1985; 89: 387-91.","journal-title":"Gastroenterology"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1172\/JCI114128","article-title":"Secretory pancreatic stone protein messenger RNA. Nucleotide sequence and expression in chronic calcifying pancreatitis","volume":"84","author":"Giorgi, D.; Bernard, J.P.; Rouquier, S.; Iovana, J.; Sarles, H.; Dagorn, J.C.","year":"1989","unstructured":"Giorgi D, Bernard JP, Rouquier S, Iovana J, Sarles H, Dagorn JC. Secretory pancreatic stone protein messenger RNA. Nucleotide sequence and expression in chronic calcifying pancreatitis. Jf Clin Invest 1989; 84: 100-6.","journal-title":"Jf Clin Invest"},{"key":"ref_10","first-page":"686","article-title":"Pancreatic stone protein in serum of patients with pancreatitis","volume":"ii:","author":"Schmiegel, W.H.; Burchert, M.; Kalthoff, H.; Thiele, H.G.; Butzow, G.H.; Klose, G.","year":"1986","unstructured":"Schmiegel WH, Burchert M, Kalthoff H, Thiele HG, Butzow GH, Klose G, et al. Pancreatic stone protein in serum of patients with pancreatitis. Lancet 1986; ii: 686.","journal-title":"Lancet"},{"key":"ref_11","first-page":"1421","article-title":"Immunochemical characterization and quantitative distribution of pancreatic stone protein in sera and pancreatic secretions in pancreatic disorders","volume":"99","author":"Schmiegel, W.; Burchert, M.; Kalthoff, H.; Roeder, C.; Butzow, G.; Grimm, H.","year":"1990","unstructured":"Schmiegel W, Burchert M, Kalthoff H, Roeder C, Butzow G, Grimm H, et al. Immunochemical characterization and quantitative distribution of pancreatic stone protein in sera and pancreatic secretions in pancreatic disorders. Gascroencerologry 1990; 99: 1421-30.","journal-title":"Gascroencerologry"},{"key":"ref_12","first-page":"680","article-title":"Pancreatic stone protein: quantification in pancreatic juice by enzyme-linked immunosorbent assay and comparison with other methods. Pancreas","volume":"4","author":"Provansal-Cheylan, M.; Mariani, A.; Bernard, J.P.; Sarles, H.; Dupuy, P.","year":"1989","unstructured":"Provansal-Cheylan M, Mariani A, Bernard JP, Sarles H, Dupuy P. Pancreatic stone protein: quantification in pancreatic juice by enzyme-linked immunosorbent assay and comparison with other methods. Pancreas 1989; 4: 680-9."},{"key":"ref_13","first-page":"253","article-title":"The pancreas, biology, patholobiology and disease","author":"In, Dagorn J.C.Lithostathine","year":"1993","unstructured":"Dagorn JC. Lithostathine. In: Go VLW, et al, eds. The pancreas, biology, patholobiology and disease. New York: Raven Press, 1993: 253-63."},{"key":"ref_14","first-page":"75","article-title":"Protein measurement with the Folin phenol reagent","volume":"192","author":"Lowry, O.H.; Rosebrough, N.J.; Farr, A.L.; Randall, R.J.","year":"1951","unstructured":"Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951; 192:75.","journal-title":"J Biol Chem"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the lead of bacteriophage T4","volume":"227","author":"Laemmli, U.K.","year":"1970","unstructured":"Laemmli UK. Cleavage of structural proteins during the assembly of the lead of bacteriophage T4. Nature 1970; 227: 680-5.","journal-title":"Nature"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/0016-5085(81)90007-X","article-title":"Characterization of human exocrine pancreatic proteins by two-dimensional isoelectric focusing\/sodium dodecyl sulphate gel electrophoresis","volume":"80","author":"Scheele, G.; Bartelt, D.; Bieger, W.","year":"1981","unstructured":"Scheele G, Bartelt D, Bieger W. Characterization of human exocrine pancreatic proteins by two-dimensional isoelectric focusing\/sodium dodecyl sulphate gel electrophoresis. Gastroenterology 1981; 80: 461-73.","journal-title":"Gastroenterology"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/0003-2697(81)90281-5","article-title":"'Westem blotting'. Electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to unmodified nitrocellulose and radiolographic detection with antibody and radioiodinated protein A","volume":"112","author":"Bumette, W.N.","year":"1981","unstructured":"Bumette WN. 'Westem blotting'. Electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to unmodified nitrocellulose and radiolographic detection with antibody and radioiodinated protein A. Anal Biochem 1981; 112: 195.","journal-title":"Anal Biochem"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1136\/gut.25.10.1107","article-title":"Pancreatography in chronic pancreatitis: international definitions","volume":"25","author":"Axon, A.T.R.; Classen, M.; Cotton, P.B.; Cremer, M.; Frenny, P.C.; Lee, W.R.","year":"1984","unstructured":"Axon ATR, Classen M, Cotton PB, Cremer M, Frenny PC, Lee WR. Pancreatography in chronic pancreatitis: international definitions. Gut 1984; 25: 1107-12.","journal-title":"Gut"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1097\/00006676-199107000-00015","article-title":"Definition and classification of chronic pancreatitis","volume":"6","author":"Sarles, H.","year":"1991","unstructured":"Sarles H. Definition and classification of chronic pancreatitis. Pancreas 1991; 6: 470-4.","journal-title":"Pancreas"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/0378-4347(92)80202-2","article-title":"Separation of rat pancreatic secretory proteins by cation-exchange fast protein liquid chromatography","volume":"576","author":"Fuchs, M.J.; Keim, V.","year":"1992","unstructured":"Fuchs MJ, Keim V. Separation of rat pancreatic secretory proteins by cation-exchange fast protein liquid chromatography. Jf Chromatogr 1992; 576: 287-95.","journal-title":"Jf Chromatogr"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1097\/00006676-199005000-00004","article-title":"Resolution of human exocrine pancreatic juice proteins by reversed-phase high performance liquid chromatography (HPLC)","volume":"5","author":"Goke, B.; Keim, V.; Dagorn, J.C.; Arnold, R.; Adler, G.","year":"1990","unstructured":"Goke B, Keim V, Dagorn JC, Arnold R, Adler G. Resolution of human exocrine pancreatic juice proteins by reversed-phase high performance liquid chromatography (HPLC). Pancreas 1990; 5: 261-6.","journal-title":"Pancreas"},{"key":"ref_22","first-page":"265","article-title":"The use of two-dimensional gel electrophoresis and high-performance liquid chromatography for the analysis of pancreatic juice","volume-title":"The pancreas, biology, pathobiology and disease","author":"Padfield, P.J.; Scheele, G.A.","year":"1993","unstructured":"Padfield PJ, Scheele GA. The use of two-dimensional gel electrophoresis and high-performance liquid chromatography for the analysis of pancreatic juice. In: Go VLW, et al, eds. The pancreas, biology, pathobiology and disease. New York: Raven Press, 1993: 265-73."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1509","DOI":"10.1007\/BF01296390","article-title":"Differences of pancreatic stone morphology and content in patients with pancreatic lithiasis","volume":"36","author":"Mariani, A.; Bernard, M.; Provansal-Cheylan, M.; Nitsche, S.; Sarles, H.","year":"1991","unstructured":"Mariani A, Bernard M, Provansal-Cheylan M, Nitsche S, Sarles H. Differences of pancreatic stone morphology and content in patients with pancreatic lithiasis. Dig Dis Sci 1991; 36: 1509-16.","journal-title":"Dig Dis Sci"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1042\/bj2220669","article-title":"The molecular characteristics of a human pancreatic acidic phosphoprotein that inhibits calcium carbonate crystal growth","volume":"222","author":"A, De Caro; L, Multigner; H, Lafont; D, Lombardo; H, Sarles","year":"1984","unstructured":"De Caro A, Multigner L, Lafont H, Lombardo D, Sarles H. The molecular characteristics of a human pancreatic acidic phosphoprotein that inhibits calcium carbonate crystal growth. BiochemJ 1984; 222: 669-77.","journal-title":"BiochemJ"}],"container-title":["Gut"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/gut.36.4.622","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T03:03:32Z","timestamp":1764990212000},"score":1,"resource":{"primary":{"URL":"https:\/\/gut.bmj.com\/lookup\/doi\/10.1136\/gut.36.4.622"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,4]]},"references-count":24,"journal-issue":{"issue":"4","published-online":{"date-parts":[[1995,4,1]]},"published-print":{"date-parts":[[1995,4]]}},"alternative-id":["10.1136\/gut.36.4.622"],"URL":"https:\/\/doi.org\/10.1136\/gut.36.4.622","relation":{},"ISSN":["0017-5749","1468-3288"],"issn-type":[{"type":"print","value":"0017-5749"},{"type":"electronic","value":"1468-3288"}],"subject":[],"published":{"date-parts":[[1995,4]]}}}